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本文介绍选择测定复杂有机混合物中各含氧化合物的氧-火焰离子化(O-FID)检测器,该仪器是专门对氧响应的O-FID检测器,还报道了在O-FID检测器上对含氧化合物的定量校正工作。 相似文献
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反相高效液相色谱法同时测定动物血浆中泰乐松注射液的多种组分 总被引:3,自引:0,他引:3
研究了用反相高效液相色谱法分析动物血中泰乐松注射液中三种组分氯霉素、醋酸氢化泼尼松和酒石酸泰乐菌素浓度的方法,,血药由甲醇-氯仿混合液提取,采用ODS柱,以甲醇-腈-醋酸钠溶液为流动相,可变波长检测。 相似文献
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高效液相色谱法测定荧光增白剂OB 总被引:1,自引:0,他引:1
本文建立了高效液相色谱测定荧光增白剂OB,即2.5-双(5-叔丁基-2-苯并恶唑基)噻吩的方法。采用YWG C18色谱柱,四氢呋喃-水(67:33V/V)为流动相,流速1.0mL/min,紫外检测波长360nm,柱温40℃,分离时间少于11min。应用于工业产品的测定,结果满意。 相似文献
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本文研究了新水溶性5,10,15,20-四(4-甲氧基-3-磺酸苯基)卟啉[T(4-MOP)PS4]的反相高效液相色谱(HPLC)分离条件。采用ShimpackPREP-ODS半制备色谱柱,用含有10mmol/L四乙基碘化铵的乙腈-水(体积比25:75)为流动相,流速18mL/min,于418nm波长下检测。[T(4-MOP)PS4]与合成中生成的杂质组分完全分离。经此制备的卟啉纯度高,已成功地应用于自来水样中微量钴、锌、铜离子的HPLC测定中。 相似文献
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反相色谱法分离和间接光度法测定无机阴离子 总被引:1,自引:0,他引:1
在化学键合固定相ODS柱上,用Fe(Phen)_3~(2+)作流动相添加剂分离并用间接光度法检测无机阴离子。探讨了主要因素(如流动相中Fe(Phen)_3~(2+)浓度、有机溶剂浓度、pH值和离子强度等)对阴离子保留和检测灵敏度的影响。Cl~-,Br~-,NO_3~-和I~-具有良好的分离度;间接光度检测波长为Fe(Phen)_3~(2+)的最大吸收波长510nm。测定这4个离子的校正曲线的线性范围为1~160mg/L(Cl~-,Br~-,NO_3~-)和2~320mg/L(I~-);相关系数均达0.996。 相似文献
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采用粉末微电极技术改善电流型酶电极的输出性能 总被引:1,自引:0,他引:1
由于酶反应的特异性,酶电极在检测生物底物的工作中得到了较广泛的应用,然后电流型酶电极有以下的缺点,包括响应电流低,活性组份流失电极使用寿命下降以及响应电流的非线性等。本文根据粉末酶电极模型进行了动力学分析,得出在两极极端情况下粉末酶电极的电流响应动力学公式,以及由响应电流估算表观米氏常数的方法。采用了两种粉末酶电极(C-PU-GOD和C-AQ-DBF-GOD)对理论分析进行了验证。实验结果与理论推 相似文献
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5,10,15,20—四(3—溴—4—磺酸苯基)卟啉高效液相色谱法测定河水中微量… 总被引:4,自引:1,他引:4
研究了新合成的显色剂5,10,15,20-四(3-溴-4-磺酸苯基)卟啉与Co(Ⅱ)、Zn(Ⅱ)、Cu(Ⅱ)形成配合物离子的反应,在ZORBAX ODS柱上,用含20mmol/L乙酸-乙酸钠(pH6.0)和10mmol/L四乙基碘化铵的乙腈-水(27:73,V/V)流动相洗脱,在420nm波长下检测。Co(Ⅱ)、Zn(Ⅱ)、Cu(Ⅱ)螯合物在9min内获得完全分离,检测限分别为0.2ng,0.05 相似文献
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二溴羟基苯基荧光酮—乳化剂OP荧光熄灭法测定微量铬(Ⅵ) 总被引:11,自引:3,他引:11
本文基于Cr(Ⅵ)-二溴羟基苯基荧光酮(DBH-PF)-OP体系的荧光熄灭效应,提出一种测定痕量铬(Ⅵ)的新荧光方法。在pH2.4-4.1的HCl-NaAc缓冲介质和OP存在下,Cr(Ⅵ)与DBH-PF形成1:2的橙红色络合物,络合物的最大激发发工和发射波长分别是365mm和528nm。铬(Ⅵ)量在0.05-1.5μg/25ml范围内与△F呈线性关系,检测限是2.0ng/ml。方法用于电镀废液,废 相似文献
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《Spectrochimica Acta Part B: Atomic Spectroscopy》2006,61(1):88-95
A field-deployable laser-induced breakdown spectrometer for measurements in the hundreds of meters range has been presented. The system is capable of elemental analysis with no previous preparation and in near real time, with the only requirement of a free line-of-sight between the instrument and the sample. Main factors influencing LIBS performance at stand-off distances are outlined. LIBS signal is shown to depend on range of analysis, peak power, beam quality, laser wavelength and optics dimensions. A careful control of focusing conditions has been shown to be of importance to avoid interferences from air breakdown by the stand-off focused beam. 相似文献
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《液相色谱法及相关技术杂志》2012,35(13):2709-2729
Abstract The effects of the wavelength of the laser beam on the response of an evaporative light scattering detector (ELSD) are discussed. Data characterizing the response of the detector and its dependence on the sample size have been collected for six solutes, using a pulsed dye laser as light source. The experimental results suggest that there is little influence of the wavelength on the intensity of the scattered light. On the other hand, the noise decreases in proportion to the wavelength of the incident light beam. Thus, the detection limit (at constant value of the signal to noise ratio) decreases with decreasing wavelengths. The performance of the ELSD improves when a short wavelength is used. 相似文献
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In this paper, we first introduced the basic principle of fluorescence cross-correlation spectroscopy (FCCS) and then established
an FCCS setup using a single wavelength laser. We systematically optimized the setup, and the detection volume reached about
0.7 fL. The homebuilt setup was successfully applied for the study of the binding reaction of human immunoglobulin G with
goat antihuman immunoglobulin G. Using quantum dots (745 nm emission wavelength) and Rhodamine B (580 nm emission wavelength)
as labeling probes and 532 nm laser beam as an excitation source, the cross-talk effect was almost completely suppressed.
The molecule numbers in a highly focused volume, the concentration, and the diffusion time and hydrodynamic radii of the reaction
products can be determined by FCCS system.
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Translated from Chemical Journal of Chinese Universities, 2008, 29(5) (in Chinese) 相似文献
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Sydney Leach 《Journal of Chemical Sciences》1991,103(3):225-236
Energy ranges and time scales for excitation and relaxation of intramolecular processes are discussed and compared with the
operational characteristics of laser and synchrotron radiation sources. The basic physics of synchrotron radiation and undulator
emission is presented. It is shown how undulators can be used to generate short wavelength harmonics. Free electron lasers
of the Compton and Raman scattering types and the associated electron beam sources are described. The properties and applications
of free electron lasers are reviewed. 相似文献
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《Spectrochimica Acta Part B: Atomic Spectroscopy》1995,50(8):823-835
Repetitive wavelength scanning can be used as a means of detecting and correcting for unwanted background in atomic emission spectrometry (AES). This paper deals with the theory underlying such harmonic wavelength scanning in time and stresses the relative importance of the relevant parameters. A set of integrated Turbo Pascal programs for an IBM PC was developed to provide a numerical simulation for the deconvolution of a detected spectral profile during repetitive harmonic wavelength scanning. By applying this numerical analysis the theory for separating the net spectral emission signal and the background radiation by filtering specific frequency components is investigated. It is concluded that, barring the dc component, repetitive scanning over a symmetric spectral profile leads to the generation of even-numbered Fourier harmonics at the modulation frequency, while an asymmetric spectral profile generates odd-numbered Fourier harmonics. 相似文献
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The photodissociation dynamics of N2O in the wavelength region of 203-205 nm was studied by velocity map ion imaging. A speed resolution of 0.8% was obtained using standard projection imaging and subpixel centroiding calculations. To investigate N2O dissociation starting from the excited bending levels in the ground electronic state, a supersonic molecular beam and an effusive beam were used. The photoabsorption transition probability from the first excited bending level in the wavelength region of 203-205 nm was estimated to be seven times greater than that from the ground vibrational level. 相似文献
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A high-performance liquid chromatographic assay has been developed for the determination of a number of non-steroidal anti-inflammatory drugs in plasma. The samples were prepared by adding acetonitrile and perchloric acid to 200 microliter of plasma. Diclofenac, fenoprofen, ketoprofen, naproxen, phenylbutazone, piroxicam and sulindac were quantified in the supernatant produced using a mobile phase of phosphoric acid 0.03% (pH 2.5)-acetonitrile and a detecting wavelength of 254 nm. The reproducibility, linearity, precision and specificity of the assay were determined and found to be satisfactory. Alteration of the detection wavelength to 229 nm also permitted accurate determination of ibuprofen concentration in plasma. While reduction of the organic solvent content of the mobile phase and alteration of wavelength to 313 nm produced a system capable of quantifying salicylate and its metabolites in plasma and by further reducing the detecting wavelength to 237 nm, aspirin also was quantifiable. These methods have been applied in a cross-sectional study of medication compliance among rheumatoid arthritis patients treated with non-steroidal anti-inflammatory drugs. 相似文献
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《Spectrochimica Acta Part B: Atomic Spectroscopy》1998,53(5):731-739
Analytical performance of laser ablation inductively coupled plasma-atomic emission spectrometry (ICP-AES) depends critically on the interaction between the laser light and the sample. The analyte emission line intensity in ICP-AES depends on the quantity of mass ablated. The effect of laser parameters (wavelength, pulse duration, and power density) was investigated for increasing the quantity of ablated mass. For fixed laser beam energy, the ablated mass can change 2 to 3 orders of magnitude by changing the laser beam spot size on the sample. The ablated mass quantity also depends on laser pulse duration and wavelength; and on ambient gas in the sample chamber. The shorter the pulse duration and wavelength, the higher the quantity of ablated mass. By using He in the chamber, the amount of mass increases by a factor of 2 for 30 ns excimer laser ablation and by an order of magnitude for ps-laser ablation. 相似文献
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Time-integrated spatially-resolved Laser Induced Breakdown Spectroscopy (LIBS) has been used to investigate spectral emissions from laser-induced plasmas generated on steel targets. Instead of detecting spectral lines in the visible/near ultraviolet (UV), as investigated in conventional LIBS, this work explored the use of spectral lines emitted by ions in the shorter wavelength vacuum ultraviolet (VUV) spectral region. Single-pulse (SP) and dual-pulse LIBS (DP-LIBS) experiments were performed on standardized steel samples. In the case of the double-pulse scheme, two synchronized lasers were used, an ablation laser (200 mJ/15 ns), and a reheating laser (665 mJ/6 ns) in a collinear beam geometry. Spatially resolved and temporally integrated laser induced plasma VUV emission in the DP scheme and its dependence on inter-pulse delay time were studied. The VUV spectral line intensities were found to be enhanced in the DP mode and were significantly affected by the inter-pulse delay time. Additionally, the influence of ambient conditions was investigated by employing low pressure nitrogen, argon or helium as buffer gases in the ablation chamber. The results clearly demonstrate the existence of a sharp ubiquitous emission intensity peak at 100 ns and a wider peak, in the multi-microsecond range of inter-pulse time delay, dependent on the ambient gas conditions. 相似文献
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Dr. Shigang Wan Hongqi Zhou Jinxiong Lin Prof. Dr. Wei Lu 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(22):8494-8498
A proof-of-principle prototype of a volumetric 3D-displaying system is demonstrated by utilizing the photo-activated phosphorescence of two long-lived phosphorescent metal-porphyrins in dimethyl sulfoxide (DMSO), a photochemically deoxygenating solvent. The first phosphorescent sensitizer, Pt(TPBP), absorbs a light beam with a wavelength of 635 nm, and the sensitized singlet oxygen is scavenged by DMSO. The second phosphorescent emitter, Pt(OEP), absorbs a light beam with a wavelength of 532 nm and visibly phosphoresces only in the deoxygenated zone generated by the first sensitizer. The phosphorescent voxels, 3D images, and animations are well-defined by the intersections of the 635-nm and 532-nm light beams that are programmable by tuning of the excitation-power densities, the beam shapes, and the kinetics. As a pivotal selection rule for the phosphorescent molecular couple used in this 3D-displaying system, their absorptions and emissions must be orthogonal to each other, so that they can be excited and addressed independently. 相似文献