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1.
Effect of the luminol signal enhancer selection on the curve parameters of an immunoassay and the chemiluminescence intensity and kinetics 总被引:1,自引:0,他引:1
Yannis Dotsikas 《Talanta》2007,71(2):906-910
In the present study, three luminol signal enhancers {4-methoxyphenol, 4-hydroxybiphenyl and 4-(1H-pyrrol-1-yl)phenol} were utilized in the chemiluminescence (CL) substrate solution of horseradish peroxidase (HRP). The latter was applied in a heterogenous enzyme immunoassay that has been previously described. The employment of these molecules greatly affected important assay parameters, such as detection limit and the range of the calibration curve and the results were compared with those obtained from other two similar enhancers that have been described from our group. Practically, the use of a novel enhancer, even if this is a slightly changed 4-substituted phenol derivative, can affect assay properties so dramatically, one can assume that another substrate/enzyme system was applied. Furthermore, the use of different luminol signal enhancers in the luminol/HRP/H2O2 system affected not only the intensity of the obtained signal, which is well known, but also its kinetics. It was monitored that the stronger intensity was combined with a more rapid decrease of the CL signal. 相似文献
2.
ZHUANG Hui-Sheng WANG Qiong-E ZHANG FanDepartment of Chemistry Fuzhou University Fuzhou Fujian China.HUANG Qiao-Jia TANG Yu-ChaiFuzhou General Hospital Nanjing Military Region Fuzhou Pujian China 《中国化学》1997,15(2):123-129
A new chemilurninescence label N-(β-carboxypropionyl)luminol (CPL) was used to label sheep anti-human IgG (SaHIgG).The labeled antibody was stable and could be detected at least down to 10-17~10-16 mol.The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG.There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling. 相似文献
3.
将邻羟苯基引入1,2,3-三唑结构中, 设计合成了10个1-(4-取代苯基)-4-苯基-5-取代-1,2,3-三唑类衍生物. 首先, 以对位取代的芳胺为原料, 经重氮化、叠氮化、闭环和缩合反应制得1-(4-取代苯基)-4-苯基-5-水杨醛亚胺-1,2,3-三唑类衍生物(3a~3e), 再用硼氢化钠还原制得1-(4-取代苯基)-4-苯基-5-(2-羟基苄基)氨基-1,2,3-三唑类衍生物(4a~4e). 目标化合物的结构经核磁、IR及元素分析确认. 抑菌活性测试表明, 当质量浓度为0.1 mg/L时, 除化合物3e和4e外, 所有化合物对白色念球菌的抑菌率均达95%以上, 对大肠杆菌的抑菌率达85%以上, 具有强抑菌活性, 表明该类化合物在抗菌药物开发方面有重要应用价值. 相似文献
4.
1-(4-硝基苯基)-3-(2-吡嗪)-三氮烯的合成及与镉(Ⅱ)的显色反应 总被引:1,自引:0,他引:1
报道了显色剂1-(4-硝基苯基)-3-(2-吡嗪)-三氮烯化合物的合成及其与镉(Ⅱ)的显色反应。在非离子表面活性剂Triton X-100存在下,pH 10.5的Na2B4O7-NaOH的缓冲介质中,该试剂能与镉(Ⅱ)与发生显色反应,形成摩尔比为4∶1的黄棕色配合物,配合物在波长453 nm处有最大吸收峰,表观摩尔吸光系数ε为6.90×104L.mol-1.cm-1,镉(Ⅱ)质量浓度在0~0.56μg/mL范围内遵守比尔定律。用拟定方法测不同废水中的镉(Ⅱ)。 相似文献
5.
新显色剂1-(4-硝基苯基)-3-(5-氯吡啶)三氮烯的合成及其与镉的显色反应 总被引:4,自引:0,他引:4
报道了1-(4-硝基苯基)-3-(5-氯吡啶)三氮烯(NPCPDT)的合成及其与镉的显色反应研究。在Triton X-100的存在下,pH 11.5的Na2B4O7-NaOH缓冲溶液中,该试剂能与镉发生显色反应,镉与NPCPDT形成摩尔比为1∶3的黄色配合物,在440 nm处有一最大吸收,在535 nm处有一最大负吸收。以440 nm为参比波长,535 nm为测量波长进行双波长测定,表观摩尔吸光系数为2.68×105L.mol-1.cm-1,镉的质量浓度在0~0.48μg/mL范围内符合比尔定律。用拟定方法测定废水和环境水样中的微量镉。 相似文献
6.
Thetfiazenereagentswereusedforthedeterminationofcadmium,mercuryandzinc.Wehavesynthesizedthetriazenereagentscontainingheterocyclicring'-'.Recently,annewreagentEtOBTNPTwassynthesized.Becausethereagentcontainsanelectronpropellinggroup(-OCH,CH,)inoneendandanelectronwithdrawinggroup(-NO,)intheotherend,sotheconjugatesystemisenlargedinthemolecularstructure,thisresultinginadvantageofmigrationofelectroniccloud.ThesensitivityofthecolourreactionofEtOBTNPTwithmercuryissuperiortoothertfiazenereage… 相似文献
7.
Yang Y Yu C Zhou M Pang N Li N Nie H Liao J Bai Y Liu H 《Journal of chromatography. A》2011,1218(37):6505-6510
4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) with one chiral center at the carbinol is a major metabolite of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). As tobacco specific N-nitrosamines (TSNAs), NNK and NNAL are the most pulmonary carcinogens in tobacco products and smoke. In this paper, a chiral CE method modified with highly sulfated β-cyclodextrin (S-β-CD) was developed to investigate the stereoselective formation of NNAL from NNK in vitro in normal human bronchial epithelial (NHBE) cells. Combined with solid phase extraction (SPE) of the cell samples, NNK and NNAL enantiomers were baseline separated under the proposed CE conditions, with satisfactory recoveries (72.5-113% for NNK and (±)-NNAL) and low limits of detection (LOD, 2.5-3 μg/mL for NNK and (±)-NNAL). The cytotoxicity of NNK in NHBE cells was investigated through the cell counting kit (CCK) assay and proved to be highly dependent on the NNK's concentration. The metabolic results obtained from CE analysis demonstrated that NNK was preferentially metabolized to (+)-NNAL through carbonyl reduction. Meanwhile, the ratio of [(+)-NNAL]/[(-)-NNAL] was independent of NHBE cells' incubation time with NNK, but could be changed according to the original incubation concentration of NNK. This chiral CE method could be useful for the study on toxicology and metabolic transformations of related TSNAs. 相似文献
8.
The title compound(E)-2-(4-tert-butyl-5-(4-methoxybenzyl)thiazol-2-ylimino)me-thylphenol was synthesized by the reaction of 5-(4-methoxybenzyl)-4-tert-butylthiazol-2-amine with salicylaldehyde=and its crystal structure was determined by single-crystal X-ray diffraction.The crystal belongs to the monoclinic system=space group P21/c with a=5.9362(8)=b=11.5070(15)=c=29.460(4)=β=97.326(3)°=V=1995.9(5)3=Z=4=F(000)=808=C22H24N2O2S=Mr=380.49=Dc=1.266 g/cm3=S=1.031=μ=0.181 mm--1=the final R=0.0474 and wR=0.1441 for 4327 observed reflections(I > 2σ(I)).Intramolecular O-H…N hydrogen bond is observed in the crystal.The preliminary bioassay showed that the title compound exhibits 95% inhibition rate against Rhizoctonia solani at the test concentration of 500 mg/L. 相似文献
9.
目的:合成新型化合物d-4-萜品醇丁酸脂(d-4-T-C4),探究其对氟比洛芬(SR-FP)的促透作用并研究其促透机制。方法:以d-4-萜品醇(d-4-TER)、丁酸为原料,采用酰氯酯化法合成d-4-T-C4,并通过MS和1H-NMR进行结构表征。同时进行体外渗透实验,考察d-4-T-C4对SR-FP的体外促透活性,并利用红外光谱法和分子对接技术研究其促透机制。结果:加入d-4-T-C4时SR-FP的24 h累计透过量(Q24h)为383.5±48.75 ?g·cm-2,分别为对照组和d-4-TER组的3.12、1.34倍,均有显著差异(P<0.05)。同时通过对药物进行手性拆分可以发现d-4-T-C4对S-FP和R-FP的透过没有显著性差异(P>0.05)。通过探究促透机制发现d-4-T-C4插入角质层脂质结构域,破坏药物与神经酰胺之间的氢键相互作用,增加脂质迁移率和药物的自由能,从而促进药物的渗透。结论:d-4-T-C4对SR-FP具有显著的促透效果,有望作为新型促透剂在经皮给药系统中广泛应用。 相似文献
10.
新合成了吡啶类三氮烯显色剂1-(4-硝基苯基)-3-(3,5-二溴吡啶)三氮烯(NPDBPDT),并研究了NPDBPDT与锌的显色反应。在Na2B4O7-NaOH缓冲溶液(pH 10.0)介质中,于表面活性剂Triton X-100存在下,锌与试剂生成1∶2络合物。该络合物在450 nm处有最大正吸收峰,以540 nm为参比波长,450 nm为测量波长进行双峰双波长测定,其表观摩尔吸光系数为2.17×105L.mol-1.cm-1,锌含量在0~12μg/25mL范围内符合比耳定律。 相似文献
11.
Ling Tian TANG Yi WANG Xin Qi LIU Shao Wen HU Tai Wei CHU Xiang Yun WANG* College of Chemistry Molecular Engineering Peking University Beijing 《中国化学快报》2005,16(3):345-348
Benzimidazole compounds have attracted a renewed interest recently owing to theirpotential applications in high-performance composite materials, electronic chemicals,photosensitive materials, and their special potentials in biological and/or medicinalapplication1,2. Typically, aromatic compounds with near planar structures and contain-ing hydrogen-donor groups or groups, which are capable of being protonated, havespecial interactions with DNA via intercalation, hydrogen-bonding, and so on3.Me… 相似文献
12.
合成了1-(4-硝基苯基)-3-(3,5-二溴吡啶)三氮烯(NPDBPDT),并研究了NPDBPDT与汞的显色反应.在Na2B4O7-NaOH缓冲溶液(pH11.0)介质中,在Triton X-100存在下Hg(Ⅱ)与试剂生成络合物(1∶2).络合物在450 nm处有最大正吸收峰,表观摩尔吸光系数为1.36×105 L·mol-1·cm-1,在535 nm处有最大负吸收峰,表观摩尔吸光系数为1.57×105 L·mol-1·cm-1,以535 nm为参比波长,以450 nm为测量波长进行双波长测定,表观摩尔吸光系数为2.93×105 L·mol-1·cm-1.汞量在0~0.48 μg/mL范围内符合比耳定律. 相似文献
13.
14.
含丁三醇端基富电子醚链与阳离子环蕃超分子准轮烷的表征和性能研究 总被引:1,自引:0,他引:1
以4-(2-(4-(苄氧基)苯氧基)乙氧基)-1,2,3-丁三醇(C)为富电子供体的醚链,与缺电子联吡啶大环化合物环双(百草枯-亚苯基)四阳离子环蕃(CPQT)和四氟取代环蕃(4FCPQT)自组装形成超分子准轮烷C(CPQT)和C(4FCPQT),并利用1HNMR的化学位移变化来研究两种不同准轮烷在温度变化时它们的相互作用。实验结果表明,由于富电子供体的一端含有3个羟基,易和缺电子联吡啶大环形成氢键,因此醚链的丁三醇端不能进入大环;缺电子联吡啶大环的一个苯环上的氢被氟取代后,由于电场力的作用,使富电子供体进入大环的概率相对降低,并使富电子醚链供体穿入大环的位置发生"偏心"作用。 相似文献
15.
The crystal structure of four 1-oxo-4-(substituent)-1-phospha-2,6,7-trioxacyclo(2,2,2) octane have been determinded by dimention single-crystal X-ray analysis. All intensity data were collected with an Enraf-Nonius CAD4 diffractometer, using graphite monochromated Cu-K_α radiation and ω/2θ scan, and corrected for LP and emperical absorption factors. All crystal structures were solved by MULTAN and refined by full matrix least-squares with lsotropic thermal vibration factors for H and anisotropic for the others.Crystal data, (I) X=NH, R=H, ... 相似文献
16.
用DCNPNPT测定江水中季铵盐型阳离子表面活性剂 总被引:4,自引:0,他引:4
利用三氮烯类试剂与季铵盐型阳离子表面活性剂形成离子缔合物可用于季铵盐型阳离子表面活性剂的测定。如用HDNPAPT[1]、HDAA[2]测定CTMAB、CPB,但其灵敏度有待提高。本文研究了显色剂1 (2,6 二氯 4 硝基苯) 3 (4 硝基苯) 三氮烯(DCNPNPT)[3]与阳离子表面活性剂的显色反应,结果表明,DCNPNPT与CTMAB、CPB反应的表观摩尔吸光系数分别为3 93×104L·mol 1·cm 1,和4 16×104L·mol 1·cm 1,是目前报道的用光度法测定季铵盐型阳离子表面活性剂较灵敏的体系之一。用此法测定了瓯江水中微量阳离子表面活性剂CTMAB、CPB的… 相似文献
17.
1-(4-硝基苯)-3-(3-甲基吡啶)-三氮烯合成及其与铜的显色反应 总被引:1,自引:0,他引:1
报道了新显色剂 1-(4-硝基苯)-3-(3-甲基吡啶)-三氮烯(NPMPDT)的合成及其与铜的显色反应.在OP的存在下,pH 10.5的Na2B4O7-NaOH缓冲溶液中,该试剂能与铜发生显色反应,铜与NPMPDT形成摩尔比为2∶1型的黄色配合物,在460 nm处有一最大正吸收,在535 nm 处有一最大负吸收.以460 nm为参比波长,535 nm为测量波长进行双波长测定,其表观摩尔吸光系数ε=2.18×105 L·mol-1·cm-1,铜的质量浓度在0~0.48 μg/mL 范围内符合比尔定律,用本法测定大米和面粉中微量的铜,结果满意. 相似文献
18.
SPAPT-CTMAB-AS显色反应的研究及应用 总被引:2,自引:0,他引:2
合成的新试剂1-(4-磺酸基苯基)-3-[4-(苯基偶氮)苯基]-三氮烯(SPAPT)与阳离子表面活性剂溴化十六烷基三甲铵(CTMAB)和阴离子表面活性剂(AS)在三乙醇胺调节的碱性(pH
11.00~11.20)介质中形成三元显色配合物。配合物最大吸收λ位于590 nm,测定阴离子表面活性剂十二烷基苯磺酸钠(DBOSO 相似文献
19.
M. A. Sibiryakova G. F. Muzychenko T. P. Kosulina K. S. Pushkareva 《Chemistry of Heterocyclic Compounds》2006,42(7):875-881
The interaction has been investigated of 1-(4-nitrophenyl)pyrrolin-2-one with aromatic aldehydes in acetic anhydride in the
presence of catalytic amounts of pyridine. 1-(4-Nitrophenyl)pyrrolinium perchlorate has been synthesized and was put into
a condensation reaction with aromatic aldehydes. As a result 5-arylidene-1-(4-nitrophenyl)pyrrolin-2-onium perchlorates were
obtained.
__________
Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 7, pp. 1015–1021, July, 2006. 相似文献
20.
1-(3-硝基苯基)-3-[4-(苯基偶氮)苯基]-三氮烯与阳离子表面活性剂显色反应的研究及应用 总被引:4,自引:0,他引:4
在NaOH碱性介质中,1 (3 硝基苯基) 3 [4 (苯基偶氮)苯基] 三氮烯(m NPPAPT)分别与阳离子表面活性剂(CS)溴化十六烷基三甲铵(CTMAB)、溴化十二烷基二甲基苄铵(DDMBAB)、溴化十六烷基吡啶(CPB)和溴化十四烷基吡啶(TPB)反应显色形成离子配合物,它们与m NPPAPT形成离子配合物比均为2∶1。CTMAB、CPB、TPB、DDMBAB与m NPPAPT形成配合物最大吸收波长分别位于538、538、625、637nm,表观摩尔吸光系数达(103~104)L·mol-1·cm-1。研究了测定微量阳离子表面活性剂的条件,建立了新的分析方法,并应用于实样分析。 相似文献