The Neutral Part of the Bark of Pinus Luchuensis Mayer

The neutral part of the acetone extract from the bark of Pinus luchuensis Mayer has been investigated and found to consist of alkanes (C₂₂–C₃₄) and triterpenes of serratene type. The triterpenes are 3β–methoxyserrat–14–en–21–one, serrat–14–en–3, 21–dione, 3β–hydroxyserrat–14–en–21–one, 3β–21α–dimethoxyserrat–l4–ene and 3β–methoxyserrat–14–en–21α–ol.     

Alkaloids of Senecio Taiwanensis Hayata and Senecio Morrisonensis Hayata

Two alkaloids, rosmarinine (I) from Senecio taiwanensis Hayata and integerrimine(II) from Senecio morrisonensis Hayata, were isolated and identified respectively.     

微流控芯片快速鉴定黄山松DNA多态性片段

黄山松是中国山区特有的优质针叶松,有关其遗传图谱的建立一直受到人们的关注,然而迄今为止,运用分子标记法来建立其遗传图谱尚未见报道．随机引物扩增DNA多态性标记(简称RAPD)可快速提供联锁信息,尤其适用于建立针叶树类的基因图谱,通常采用平板凝胶电泳对多态性的     

New Triterpenes from the Bark of Western White Pine (Pinus monticola DOUGL.)

Eleven new triterpenes with the lanostene-type skeleton were isolated from the benzene extract of western white pine bark. Their structures were determined mainly on the basis of physical and spectral data.     

Cytotoxicity Effect of Constituents of Pinus taiwanensis Hayata Twigs on B16-F10 Melanoma Cells

Pinus taiwanensis Hayata (Pinaceae) is an endemic plant in Taiwan. According to the Chinese Materia Medica Grand Dictionary, the Pinus species is mainly used to relieve pain, and eliminate pus and toxicity. In this study, nineteen compounds were isolated from the ethyl acetate layer of the ethanolic extract of P. taiwanensis Hayata twigs using bioassay-guided fractionation, and their anti-melanoma effects were investigated through a B16-F10 mouse melanoma cell model. The structures of the purified compounds were identified by 2D-NMR, MS, and IR, including 1 triterpenoid, 9 diterpenoids, 2 lignans, 4 phenolics, 1 phenylpropanoid, 1 flavonoid, and 1 steroid. Among them, compound 3 was found to be a new diterpene. Some of the compounds (2, 5, 6, 17, 18) showed moderate cytotoxicity effects. On the other hand, the anti-melanoma effect was no better than that from the original ethyl acetate layer. We presumed it resulted from the synergistic effect, although further experimentation needs to be performed.     

Hydroxypropylation of Polyphenol-Rich Alkaline Extracts from Pinus radiata Bark and Their Physicochemical Properties

Pinus radiata bark is a rich source of polyphenols, which are mainly composed of proanthocyanidins. This study aimed to utilize P. radiata bark as a polyol source for bio-foam production in the future. Polyphenol-rich alkaline extracts (AEs) from P. radiata bark were prepared by mild alkaline treatment and then derivatized with propylene oxide (PO). Hydroxypropylated alkaline extracts (HAEs) with varying molar substitutions (MS 0.4–8.0) were characterized by FT-IR, NMR, GPC, TGA, and DSC. The hydroxyl value and solubility in commercial polyols were also determined. The molecular weights of the acetylated HAEs (Ac-HAEs) were found to be 4000 to 4900 Da. Analyses of FT-IR of HAEs and ¹H NMR of Ac-HAEs indicated that the aromatic hydroxyl groups were hydroxypropylated and showed an increase in aliphatic hydroxyl group content. The glass transition temperature (T_g) of AE and HAEs were 58 to 60 °C, showing little difference. The hydroxyl value increased as the hydroxypropylation proceeded. Although salts were produced upon neutralization after hydroxypropylation, HAEs still showed suitable solubility in polyether and polyester polyols; HAEs dissolved well in polyether polyol, PEG#400, and solubility reached about 50% (w/w). This indicated that neutralized HAEs could be directly applied to bio-foam production even without removing salts.     

The Chemical Constituents of the Peel of Citrus Tankan Hayata

Seven kinds of flavones, β-sitosterol, β-sitosterol-D-glucoside, hesperidin, limonin, ferulic acid and succinic acid were isolated from the fruit peel of Citrus tankan Hayata.     

The Structure of Suginal from the Wood of Juniperus Formosana Hayata

The detailed investigation of the acetone extract from the wood of Juniperus formosana Hayata gave a new diterpene aldehyde which was elucidate as suginal on the basis of chemical and spectroscopic evidence.     

The Constituents of the Bark of Armand Pine

Twenty three compounds were isolated from the bark of Pinus armandii. These compounds include nine serratenes (1-9), a norserratenone 10, eight phenols 11-18, two flavonoids 19-20, one lignan 21, a diterpene 22 and a novel triterpendiol acetonide 23. Their structures were determined from their physical and spectral properties.     

The Structure of Taiwanin F from Taiwania Cryptomerioides Hayata

Reinvestigation of the nonvolatile fraction of the acetone extractive from the heartwood of Taiwania cryptomerioides Hayata afforded taiwanin F which was established as 11-hydroxy-7-oxohinokiol on the basis of spectral data and its synthesis from hinokiol. In addition, six known compounds, taiwanin A, savinin, taiwanin C, hinokiol, taiwanin E and diphyllin were also isolated from the same fraction.     

Neuroprotective and Anti-Inflammatory Effects of Pinus densiflora Bark Extract in Gerbil Hippocampus Following Transient Forebrain Ischemia

Korean red pine (Pinus densiflora) belongs to the Genus Pinus, and its bark contains a great amount of naturally occurring phenolic compounds. Until now, few studies have been conducted to assess the neuroprotective effects of Pinus densiflora bark extract against brain ischemic injury. The aim of this study was to investigate the neuroprotective effects of pre-treatment with the extract in the hippocampus following 5-min transient forebrain ischemia in gerbils. Furthermore, this study examined the anti-inflammatory effect as a neuroprotective mechanism of the extract. Pinus densiflora bark was extracted by pure water (100 °C), and this extract was quantitatively analyzed and contained abundant polyphenols, flavonoids, and proanthocyanidins. The extract (25, 50, and 100 mg/kg) was orally administered once a day for seven days before the ischemia. In the gerbil hippocampus, death of the pyramidal neurons was found in the subfield cornu ammonis 1 (CA1) five days after the ischemia. This death was significantly attenuated by pre-treatment with 100 mg/kg, not 25 or 50 mg/kg, of the extract. The treatment with 100 mg/kg of the extract markedly inhibited the activation of microglia (microgliosis) and significantly decreased the expression of pro-inflammatory cytokines (interleukin 1β and tumor necrosis factor α). In addition, the treatment significantly increased anti-inflammatory cytokines (interleukin 4 and interleukin 13). Taken together, this study clearly indicates that pre-treatment with 100 mg/kg of Pinus densiflora bark extract in gerbils can exert neuroprotection against brain ischemic injury by the attenuation of neuroinflammatory responses.     

Constituents of the Peel of Citrus Depressa Hayata

Eleven known compounds; methyl-3,4-dihydroxycinnamate, pyrocatechuic acid, isoimperatorin, naringenin, umbelliferone, pyrocatechol, meranzin hydrate, neohesperidin, narirutin, friedelin, β-sistosterol were isolated and characterized from the peel of Citrus depressa Hayata.     

Studies on the Constituents of Paris Formosana Hayata Part II

Methyl palmitate (I), methyl stearate (II), stigmasterol (III), β-sitosterol (IV), (O -acyl)-β-D -glucopyranosyl-(1→3)-stigmasterol (V), (O -acyl)-β-D -glucopyranosyl-(1→3)-β-sitosterol (VI), β-D -glucopyranosyl-(1→3)-stigmasterol (VII), β-D -glucopyranosyl-(1→3)-β-sitosterol (VIII), β-D -ecdysone (IX), diosgenin-3-α-L -rhamopyranosyl-(1→2)-[α-L -arabinofuranosyl-(1→4)]-β-D -glucopyranoside (X), diosgenin-3-O -β-chacotrioside (dioscin) (XI), and diosgenin-3-O -α-L -rhamnopyranosyl-(1→4)-α-L -rhamnopyranosyl-(1→4)-[α-L -rhamnopyranosyl-(1→2)]-β-D -glucopyranoside (XII) were isolated and characterized from the stems of Paris formosana Hayata (Liliaceae).     

Studies on the Components of Formosan Cirsium Species.: Part III Flavonoids of Cirsium Kawakamii Hayata and Cirsium Wallichii DC

A flavone glycoside, luteolin-7-glucoside (II) was isolated from the aerial portion of Cirsium kawakamii Hayata, and a dibasic acid, fumaric acid (III) was isolated from the aerial portion of C. wallichii DC.     

Cadinane-type sesquiterpenes from the roots of Taiwania cryptomerioides Hayata

Five new cadinane-type sesquiterpenes, 15-acetoxy-T-muurolol (1), isokhusinodiol (2), cadin-10(14)-ene-4beta,5alpha-diol (3), cadinane-4beta,5alpha,10beta-triol (4), and muurolane-4beta,5beta,10beta-triol (5), together with five known compounds, T-cadinol (6), T-muurolol (7), alpha-cadinol (8), delta-cadinol (9), and khusinodiol (10), were isolated from the roots of Taiwania cryptomerioides. The structure of the new constituents were elucidated through chemical and spectral studies.     

New Lignans from the Roots of Taiwania cryptomerioides Hayata

The five new lignans designated 3′,4′‐de‐O‐methylenehinokinin ( 1 ), taiwaninolide ( 2 ), 8′‐hydroxysavinin ( 3 ), isoguamarol ( 4 ), and 4′‐O‐methylsalicifolin ( 5 ), as well as the new 4‐(3,4‐dimethoxybenzyl)dihydro‐3‐(4‐hydroxybenzyl)furan‐2(3H)‐one ( 6 ) were isolated from the roots of Taiwania cryptomerioides, besides the three known compounds hinokinin ( 8 ), savinin ( 9 ), and 3,4‐de‐O‐methylenehinokinin ( 7 ). The structures of the new constituents were elucidated through chemical and spectral studies. A compound previously isolated from the heartwood of Chamaecyparis obtusa var. formosana was assigned structure 1 ; however, this structure has now been revised to be 3,4‐de‐O‐methylenehinokinin ( 7 ).     

Constituents of Roots of Rubia Lanceolata Hayata

A naphthohydroquinone and eight anthraquinones were isolated from the roots of Rubia lanceolata Hayata. Their structures were assigned as mollugin ( 1 ), l-hydroxy-2-methyl-9,10-anthraquinone ( 2 ), 2-methylquinizarin ( 3 ), 3-carbomethoxy-l-hydroxy-9,10-anthraquinone ( 4 ), lucidin ethyl ether ( 5 ), rubiadin ( 6 ), alizarin ( 7 ), ω-hydroxypachybasin ( 8 ) and digiferruginol ( 9 ) on the basis of spectral evidence. Among them, compounds 8 and 9 were isolated for the first time from Rubia species.     

The constituents of the root and stem of Aristolochia cucurbitifolia Hayata and their biological activity

Four new compounds, three phenanthrene derivatives, aristolochic acid-III methyl ester (1), cepharanone C (2), and sodium 7-hydroxyl-8-methoxyaristolate (3), and the benzoate derivative, sodium 3,4-dimethoxybenzoate (4), together with 53 known compounds were isolated and characterized from the fresh root and stem of Aristolochia cucurbitifolia. Their structures were elucidated by spectral analyses and chemical transformations. The cytotoxicity and antiplatelet activity of the isolated compounds are also discussed.