共查询到19条相似文献,搜索用时 171 毫秒
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高效毛细管电泳安培检测的进展 总被引:4,自引:4,他引:4
本文对高效毛细管电泳电化学检测方法中的安培检测进行了评述,安培检测具有灵敏度高,选择的特点,安培检测根据毛细管内径的大小有离柱安培检测和柱端安培检测,近年来脉冲安培分析法、化学修饰电极也已被引入毛细管电泳电化学检测。 相似文献
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毛细管电泳的微机化安培法检测 总被引:5,自引:1,他引:5
毛细管电泳的微机化安培法检测胡深,李培标,程介克(武汉大学化学系,武汉,430072)关键词毛细管电泳;微电极;安培法检测;微型计算机安培法电化学检测器用于毛细管电泳有独特的优点,如线性范围宽,选择性好,可以进行生物微环境电活性物质的分离检测[1,2... 相似文献
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本文建立了一种简单快速的毛细管电泳安培检测法分离检测黄连中的黄连碱、盐酸小檗碱、巴马汀、和药根碱.以150 μm的铂电极为工作电极,考察并优化了影响分离和检测的条件.在80 mmol/L磷酸盐缓冲液中添加50%甲醇(pH 6.0),分离电压15 kV,检测电位1.2 V (vs.Ag/AgCl)的条件下,黄连碱、盐酸小檗碱、巴马汀和药根碱在8min内获得良好分离.黄连碱、盐酸小檗碱、巴马汀和药根碱的峰电流面积和浓度分别在1.0×10-5~2.0×10-7,1.0×10-5 ~8.0×10-8 mol/L,1.0×10-5~1.0×10-7 mol/L和1.0×10-5~2.0×10-7mol/L范围内呈良好的线性关系.检出限(S/N=3)低达10-8mmol/L.方法应用于微波辅助溶剂提取黄连中生物碱的测定,回收率在97.0%~104%,RSDs≤3.8%,结果满意. 相似文献
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Summary A method based on a linear model enabling the efficient determination of the ionization constants (K
a) of saccharides by capillary zone electrophoresis with amperometric detection has been demonstrated. TheK
a values obtained from the plots of the reciprocal effective mobility against the inverse concentration of sodium hydroxide
were in agreement with literature values. 相似文献
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Enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) were determined by capillary zone electrophoresis (CZE) with end-column amperometric detection. The effect of several factors, such as pH and concentration of running buffer solution, separation voltage, injection time, and working potential, on CZE were investigated to establish the optimal conditions of separation and detection. Under a given set of conditions (pH 8.00 phosphate buffer solution (20 mmol/L); +0.95 V for the working potential; 18 kV for the separation voltage; sample injection at 18 kV for 10 s), the compounds investigated can be well separated and detected within 8 min. Excellent linearity was observed between peak currents and concentration of analytes in the range from 0.034 to 70.0 mg/kg for these two compounds. The detection limits (S/N= 3) for enrofloxacin and ciprofloxacin were 13.68 mg/kg and 14.35 mg/kg, respectively, which were about 7-fold lower than the maximum residue limits (MRLs) established by the European Union. A simple sample pretreatment method was developed and proved to be effective in obtaining good recoveries and short analysis time. The developed CE-AD method was simpler, faster, and less cost intensive than other reported methods, and allows the determination of ENR and its metabolite CIP in contaminated eel liver samples and other animal tissue samples at the required maximum residue limits. 相似文献
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Microchip capillary electrophoresis (μCE) with amperometric detection at Cu electrode benefited fast separation and direct detection of carbohydrates. The working electrode of 50-μm Cu wire attached nearly against the channel outlet—4 μm, where it benefited collecting detection current and suppressing overwhelming noise. The use of alkaline medium was essential to separating and detecting carbohydrates, which dissociated into the sensitive alcolate anions. The 10-cm serpentine chip, though lengthening the migration time, it provided better efficiency. Sucrose, cellobiose, glucose, and fructose migrated from the outlet in 400 s +2000 V. The linear calibration plots ranging from 10 to 1000 μM with regression coefficients better than 0.996 were obtained. The injection-to-injection reproducibility of 1.24% (n=7) for glucose in peak current and 0.6% for migration times were excellent. The detection limit was low, down to 2.3 μM for glucose (S/N=3) or 27.6 attomole in mass detection. 相似文献
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毛细管电泳柱端安培检测装置的研制 总被引:2,自引:1,他引:2
研制了一种新型的毛细管电泳柱端型安培检测装置。以直径为6μm的碳纤维微电极为工作电极,在自组装的ACS-2000毛细管电泳仪上,考察了用不同内径毛细管分离时分离电压对背景噪声的影响。利用该装置同时测定了3种苯二酚的异构体。 相似文献
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The fast separation capability of a novel miniaturized capillary electrophoresis with amperometric detection (CE-AD) system was demonstrated by determining sugar contents in Coke and diet Coke with an estimated separation efficiency of 60,000 TP/m. Factors influencing the separation and detection processes were examined and optimized. The end-capillary 300 microm Cu wire amperometric detector offers favorable signal-to-noise characteristics at a relatively low potential (+0.50 V vs. Ag/AgCl) for detecting sugars. Three sugars (sucrose, glucose, and fructose) have been separated within 330 s in a 8.5 cm length capillary at a separation voltage of 1000 V using a 50 mM NaOH running buffer (pH 12.7). Highly linear response is obtained for the above compounds over the range of 5.0 to 2.0 x 10(2) microg/mL with low detection limit, down to 0.8 microg/mL for glucose (S/N = 3). The injection-to-injection repeatability for analytes in peak current (RSD < 3.6%) and for migration times (RSD < 1.4%) was excellent. The new miniaturized CE-AD system should find a wide range of analytical applications involving assays of carbohydrates as an alternative to conventional CE and micro-CE. 相似文献
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A novel capillary electrophoresis and amperometric detection method was achieved by adding an electroactive additive (3,4-dihydroxybenzylamine, 3,4-DHBA) to the running buffer, so that both electroactive and non-electroactive food preservatives were simultaneously determined. Under the selected optimum conditions, four electroactive preservatives (methylparaben, ethylparaben, propylparaben and butylparaben) and two non-electroactive preservatives (potassium sorbate and sodium lactate) were well separated and sensitively detected with detection limits (S/N = 3) ranging from 1.06 × 10−8 to 2.73 × 10−6 g mL−1. This method has been successfully employed for the determination of both electroactive and non-electroactive preservatives in several food commodities. 相似文献
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Separation and determination of flavonoids in three traditional chinese medicines by capillary electrophoresis with amperometric detection 下载免费PDF全文
Wei Wang Ping Lin Lihong Ma Kaixuan Xu Xiuli Lin 《Journal of separation science》2016,39(7):1357-1362
Flavonoids are important active ingredients in many traditional Chinese medicines. In this paper, capillary electrophoresis with amperometric detection was employed to separate and detect eight flavonoids, rutin, quercetrin, quercetin, kaempferol, kaempferide, catechin, apigenin, and luteolin, in a home‐made capillary electrophoresis device. Under the separation voltage of 2000 V, the eight flavonoids could be completely separated within 33 min in 18 mM borax running buffer at pH 10.2. Good linear relationships were obtained for all analytes and the detection limits for flavonoids ranged from 0.46 to 0.85 μM. Then, the method was applied to separate and determine the flavonoids in three traditional Chinese medicines, hippophae rhamnoides, hypericum perforatum, and cacumen platycladi. Finally, rutin, kaempferol, quercetin, and quercetrin were discovered in these medicines and the concentrations ranged from 0.28 to 9.94 mg/g. The recoveries of flavonoids ranged from 84.7 to 113%, which showed the high reliability of this method. 相似文献
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<正>A high-performance capillary electrophoresis with amperometric detection(CE-AD) method has been developed for the analysis of seven bioactive ingredients,namely ferulic acid(FA),vanillin,vanillic acid,p-hydroxybenzoic acid,caffeic acid,gallic acid and protocatechuic acid,in Rhizoma Chuanxiong.The effects of several factors such as the acidity and concentration of running buffer,the separation voltage,the applied potential to working electrode and the injection time were investigated.Under the optimum conditions,the seven analytes could be well separated within 21 min at the separation voltage of 16 kV in a 60 mmol/L borax running buffer(pH 8.7).A 300μm diameter carbon disk electrode has a good response at potential of +950 mV(vs.SCE) for all analytes.Good linear relationship was established over three orders of magnitude with detection limits(S/N = 3) ranged from 3.3×10~(-7) to 6.7×10~(-9)g/mL.This proposed method has been successfully applied for the determination and comparison of different batches of Rhizoma Chuanxiong samples based on their characteristic electrochemical profiles. 相似文献