共查询到20条相似文献,搜索用时 15 毫秒
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X.B. Xu Joseph P. Nachtman Z.L. Jin E.T. Wei Stephen M. Rappaport A.L. Burlingame 《Analytica chimica acta》1982
More than 50 nitro-PAH have been tentatively identified in an extract of diesel-exhaust particulates. Identifications are based on high-resolution mass spectrometry of directly mutagenic fractions derived from sequential fractionation of the extract by both low- and high-resolution liquid chromatography. The diversity of nitro-PAH tentatively identified suggests that large numbers of such compounds are formed during or following the combustion process. 相似文献
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After deproteination of samples with trichloroacetic acid, creatine is determined by reaction with 1-naphthol and biacetyl, based on a stopped-flow method. The calibration graph is linear over the range 0–250 mg l?1, and recoveries from muscle samples are quantitative. 相似文献
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Akiyo KameyamaYusuke Shibuya Hiroshi KusuokuYoshinori Nishizawa Satoshi NakanoKuniaki Tatsuta 《Tetrahedron letters》2003,44(13):2737-2739
As the first natural product incorporating 1,3-dioxolan-4-one in spiro structure, spilacleosides A and B were isolated from Ruscus aculeatus, and the absolute structures determined. 相似文献
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A novel compound of a rare class of secondary metabolites, 3-geranyl-4-hydroxybenzoic acid derivative, methyl xerophytolate A (2), was isolated from the crude extract of Xerophyta plicata (Velloziaceae) collected in Rio de Janeiro, Brazil. The structure of 2 was elucidated using spectroscopic methods. 相似文献
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Y. Uchio 《Tetrahedron》1978,34(19):2893-2899
Two new sesquiterpene ketones, vulgarone A and B, were isolated from the essential oil of Chrysanthemum vulgare. The structures were determined based on comparison of the spectral properties with those of chrysanthenone and verbenone, and on the chemical interconversion. 相似文献
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Ramanavicius A 《Analytical and bioanalytical chemistry》2007,387(5):1899-1906
An amperometric biosensor for the determination of creatine was developed. The carbon rod electrode surface was coated with
sarcosine oxidase (SOX) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. The SOX from Arthrobacter sp. 1–1 N was purified and previously used for creation of a creatine biosensor. The natural SOX electron acceptor, oxygen,
was replaced by an redox mediating system, which allowed amperometric detection of an analytical signal at +400-mV potential. The response time
of the biosensor was less than 1 min. The biosensor showed a linear dependence of the signal vs. creatine concentration at
physiological creatine concentration levels. The optimal pH in 0.1 M tris(hydroxymethyl)aminomethane (Tris)–HCl buffer was
found to be at pH 8.0. The half-life of the biosensor was 8 days in 0.1 M Tris–HCl buffer (pH 8.0) at 20 °C.
Principal scheme of consecutively followed catalytic reactions used to design a biosensor for the determination of creatine 相似文献
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With a purpose to achieve an individual components of the pectins there was purified and separated the acidic components of the apple pectin and its chemical structure was studied using (13)C-NMR-Spectroscopy. The apple pectin was consecutively subjected to processing with hydrochloric acid and ethanol mixture, ultracentrifuging at 7000 and 40 000 rpm, ultrafiltration, fractionation on cellulose (DEAE-52) ion exchanger, eluting by the phosphate buffer. The contents of the acidic and neutral polysaccharides in an initial pectin and in the selected fractions controlled by narrow-band filter - spectroscopically in relation to E(q)=E(298)/E(315). In the (13)C-NMR-spectrums of the pectins before purifications on the ion exchanges besides the signals characteristic for homogalacturon, resonances are observed at 105.8, 79.01, 76.3, 73.9, 70.0 and 18.32 m.d., appropriate to the ramnogalacturonan residues. Resonance lines of acidic fraction in the field of 69.8, 70.6, 72.3, 80.6, 100.6 and 176.6 m.d. are referred to homogalacturonan residues and signals at 55.9, 73.3, 80.8, 101.6 and 172.6 m.d. to their methoxyl group. Minor resonance at 18.32 originated from C-6 rhamnose residues, substituted or unsubsituted with galactosyl-chains. Hence it has been shown that the apple pectin consist mainly of linear homogalacturonan and branched ramnogalacturonan macromolecules. 相似文献
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In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (CI) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on CI, SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l−1 to nmol l−1 magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation. 相似文献
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Jenn-Yuan Sheu Po-Hua Chen Wei-Chang Tseng Chang-Yu Chen Li-Yu Tsai Yeou-Lih Huang 《Analytical sciences》2003,19(6):957-960
A simple and sensitive spectrophotometric method for the determination of a thiobarbituric acid-reactive substance (TBARS) in human hair has been developed. The proposed method is based on the formation of a red-colored product by the reaction of products of lipid peroxidation with thiobarbituric acid in an acidic medium. The absorbance of the resulting red product was measured at 534 nm. The linear dynamic range was between 1.0 and 20 micromol/L. The recoveries were 98.3-105.0%, and the relative standard deviations (RSD) were 0.32-1.24, respectively. TBARS in digested hair sample was stable for 3 days at room temperature. It was found that, using this method, the hair TBARS concentration in smokers (0.116 +/- 0.030 micromol/g, n = 30) was significantly higher than that in non-smokers (0.096 +/- 0.015 micromol/g, n = 30) (p < 0.05). 相似文献
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The constitution of Chlamydocin – a metabolite of Diheterospora chlamydosporia with pronounced cytostatic activity – is proposed on the basis of chemical reactions and physical data. The synthesis of a derivative of a new amino acid is described. The biological activities of this compound and of derivatives of Chlamydocin are discussed. 相似文献
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The structure and absolute configuration of Cryptosporin ( 11 ), a metabolite of the fungus Cryptosporium pinicola LINDER has been elucidated. 相似文献
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SUN DongDong DONG WeiWei LI Xiang & ZHANG HanQing Pharmaceutical College Nanjing University of Chinese Medicine Nanjing China College of Chemistry Chemical Engineering Nanjing University of Technology Nanjing 《中国科学B辑(英文版)》2009,(5)
Two new ceramides were isolated from the 95% EtOH extract of traditional Chinese medicinal plant Isatis indigotica. Their structures were elucidated as 1-O-β-D-glucopyranosyl-(2S, 3R)-N-(2'-hydroxypentacosanoyl)-octadeca-11E-sphingenine (1) and 1-O-β-D-glucopyranosyl-(2S,3R)-N-(2'-hydroxyhe xacosanoyl)-octadeca-11E-sphingenine (2) on the basis of spectroscopic data. Their cytotoxic effects were evaluated by using MTT method. 相似文献
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Okara is a byproduct generated during tofu or soymilk production processes. Crude polysaccharide (yield 56.8%) was isolated by removing fat, protein and low molecular weight carbohydrates from initial okara. Crude okara polysaccharide was further divided into four soluble fractions and an insoluble residue fraction by extracting with 0.05 M EDTA + NH(4) oxalate, 0.05 M NaOH, 1 M NaOH and 4 M NaOH, with yields of 7.7%, 3.6%, 20.7%, 16.0% and 27.9%, respectively. Arabinose, galactose, galacturonic acid, xylose and glucose (only for the insoluble fraction) were the major constituent sugars. The primary sugar residues of okara polysaccharides were 1,4-linked β-galactopyranose, 1,5- and 1,3-linked α-arabinofuranose, 1,5-linked α-xylofuranose, 1,2-linked, 1,2,4-linked and terminal α-rhamnopyranose (or fucopyranose), and 1,4-linked β-glucopyranose (only for the insoluble fraction), indicating okara polysaccharides might contain galactan, arabinan, arabinogalactan, xylogalacturonan, rhamnogalacturonan, xylan, xyloglucan and cellulose. 相似文献
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Lin Li Min-Yong Low Xiaowei Ge Bosco C. Bloodworth Hwee-Ling Koh 《Analytical and bioanalytical chemistry》2013,405(13):4443-4450
A sildenafil analogue was detected in a functional coffee sample labelled to have male sexual performance enhancement effects. This analogue was isolated and purified by flash chromatography and preparative high-performance liquid chromatography. Its structure was elucidated using high-resolution mass spectrometry; electrospray ionization-tandem mass spectrometry; and nuclear magnetic resonance spectroscopy, ultraviolet spectroscopy, and infrared spectroscopy. Compared with sildenafil, instead of an N-methylpiperazinyl moiety, ring opening of the piperazine ring with the loss of a carbon atom resulted in a substituted benzenesulfonamide. The chemical name of this analogue is N-[2-(dimethylamino)ethyl]-4-ethoxy-3-(1-methyl-7-oxo-3-propyl-6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-5-yl)benzenesulfonamide. It is named descarbonsildenafil because it has one less carbon atom when compared with sildenafil. 相似文献
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S. Girotti M.L. Cascione S. Ghini G. Carrea R. Bovara A. Roda R. Motta R. Petilino 《Analytica chimica acta》1989
A bioluminescent flow sensor was developed for the assay of creatine kinase (CK) using firefly luciferase immobilized on a nylon coil. The CK-catalysed reaction of creatine phosphate with ADP took place in a cuvette before the injection into the bioluminescent detector coil. The response was linear from 0.1 to 100 U l? at 25°C. An advantage of the flow sensor is a detection limit of less than 0.1 U l?1, which, together with a high precision, allows determination of the CK activity in blood sera in about 5 min. The intra- and inter-assay reproducibilities (RSD) were less than 10% and the recovery range was 86–110%. The results agreed well with those obtained with a spectrophotometric method and with the normal reference values. 相似文献