共查询到20条相似文献,搜索用时 15 毫秒
1.
Kalnina I Zvagule T Gabruseva N Kirilova J Kurjane N Bruvere R Kesters A Kizane G Kirilovs G Meirovics I 《Journal of fluorescence》2007,17(6):633-638
ABM (3-aminobenzanthrrone derivative) developed at the Riga Technical University, Riga, Latvia) has been previously shown
as a potential probe for determination of the immune state of patients with different pathologies .The fist study (using probe
ABM) of peripheral blood mononuclear cells (PBMC) membranes of 97 Chernobyl clean-up workers from Latvia was conducted in
1997. Now we repeatedly examine the same (n = 54) individuals in dynamics. ABM spectral parameters in PBMC suspension, fluorescence
anisotropy and blood plasma albumin characteristics were recorded. In 1997 screening showed 5 different patterns of fluorescence
spectra, from which in 2007 we obtained only two. These patterns of spectra had never been previously seen in healthy individuals
or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc., examined by us. Patterns of ABM fluorescence
spectra are associated with membrane anisotropy and conformational changes of blood plasma albumin. We observed that in dynamics
1997–2007 the lipid compartment of the membrane became more fluid while the lipid-protein interface became more rigid. The
use of probe ANS and albumin auto-fluorescence allowed show conformational alterations in Chernobyl clean-up workers blood
plasma. It is necessary to note that all investigated parameters significantly differ in observed groups of patients. These
findings reinforce our understanding that that the cell membrane is a significant biological target of radiation. The role
of the membrane in the expression and course of cell damage after radiation exposure must be considered. So ten years dynamic
of PBMC membrane characteristics by ABM (spectral shift and anisotropy indexes) in Chernobyl clean-up workers reveal progressive
trend toward certain resemblance with those of chronic B-cell lymphoid leukemia. 相似文献
2.
A fluorescent probe, ABM, aminoderivative of benzanthrone, synthesized in the Department of Organic Chemistry of the Riga Technical University (Latvia), has been successfully used to characterize changes in the structural and functional properties of cell membranes during different pathologies. In the present study the physicochemical properties and the functional activity of the peripheral blood mononuclear cells (lymphocytes—Ly) in patients with rheumatoid arthritis (RA) were studied using the ABM probe. Intensity of the ABM fluorescence in the celi suspension, functional activity of the ly anisotropy of the membranes differ patients with different titres of rheumatoid factor in blood. Patients with seropositive RA had decreased proliferative activity and lower number of iy in blood plasma indicating greater alterations of the immunoregulating processes in these patients as compared to patients with seronegative RA. In the latter the Ly deficiency is compensated to some extent by increased proliferation activity of these cells. The ABM fluorescence intensity correlated not only with membrane anisotropy (r = 0.97, but also with the proliferation activity of the Ly (r = 0.98). The above parameters correlated with the clinical manifestations of the disease. The results indicate that the fluorescent probe ABM is useful for screening the physicochemical status of Ly membranes and the proliferation activity of these cells in RA patients. 相似文献
3.
Alexey O. Levchenko Nikolay N. Ustinovskii Vladimir D. Zvorykin 《Journal of Russian Laser Research》2010,31(5):475-480
An original absorption probe technique using a laser plume as a probe source of UV–visible continuum radiation is proposed
and tested. To our knowledge, this is the first time that laser plume emission is used as a UV–VIS (190–510 nm) probe beam
in transient absorption spectroscopy. The technique allows virtually single-shot recording of continuous fluorescence and
absorption spectra and proved its applicability even when used at a high-voltage high-current e-beam gun facility with a high
level of electromagnetic interference. The developed technique is applied to record the transient absorption spectra of e-beam-excited
Ne, Ar, and Kr gases. 相似文献
4.
We have studied the fluorescence spectra of blood plasma from patients with acute coronary syndrome, and also the effect of
therapeutic doses of in vivo ultraviolet blood irradiation (UBI) on the spectra. We have established that the maxima in the
fluorescence spectra of the original plasma samples, obtained from unirradiated blood, are located in the wavelength interval
330–340 nm, characteristic for the fluorescence of tryptophan residues. In extracorporeal UBI (λ = 254 nm), we observed changes in the shape and also both a blue and a red shift in the maxima of the fluorescence spectra,
differing in magnitude for blood plasma samples from different patients in the test group. We show that UBI-initiated changes
in the fluorescence spectra of the plasma depend on the original pathological disturbances of metabolite levels, and also
on the change in the oxygen-transport function of the blood and the acid–base balance, affecting the oxidative stability of
the plasma. We have concluded that UV irradiation, activating buffer systems in the blood, has an effect on the universal
and specific interactions of the tryptophan residue with the amino acid residues and water surrounding it. 相似文献
5.
N. Nizomov Z. F. Ismailov É. N. Kurtaliev Sh. N. Nizamov G. Khodzhaev L. D. Patsenker 《Journal of Applied Spectroscopy》2006,73(3):432-436
We have studied the effect of blood serum albumin on the absorption and fluorescence spectra of rhodamine C (RC), rhodamine
6G (R6G), and rhodamine 3B (R3B). Interaction of the dye with protein is assessed using the binding parameters: binding constants
and concentrations of binding sites. We have studied the effect of temperature on the binding parameters. We have observed
that heating a mixture of the dye solution with protein for 30 min leads to an increase in the binding constant for rhodamine
3B with protein by a factor of 2, while the concentration of binding sites increases by a factor of 2.3. This is explained
by features of the globular protein structure and a change in its conformation when heated. We have shown that rhodamine 3B
at a concentration of 10−5 M is the most effective among the studied rhodamine dyes for application as a fluorescent probe when studying conformational
changes in blood serum protein.
Report given at the Third International Conference on Liquid State Physics: Current Problems, May 27–31, 2005, Kiev, Ukraine.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 3, pp. 380–384, May–June, 2006. 相似文献
6.
We present the results of a spectral study of the effect of low-intensity laser radiation on the molecular structure of blood
and blood components. Analysis of the Fourier transform IR absorption spectra of blood confirmed the changes we observed previously
in the oxygen transport characteristics of blood with intravenous exposure to the emission from a He-Ne laser. We show that
structural and conformational changes in the hemoglobin tetramer, initiated by laser-induced photoreactions between Hb and
oxygen, lead to characteristic changes in the shape and intensity of the IR bands for NH stretching vibrations, and also the
amide I and amide II absorption bands. In the IR spectra of irradiated blood samples, we note increased absorption in the
bands for stretching vibrations of the phosphate groups (945–1280 cm−1), which is evidence for an increase in the nucleic acid content (DNA, RNA). In the spectra of plasma and erythrocytes prepared
from irradiated blood, there are no changes in this region of the IR spectrum. At the same time, in the IR spectra of samples
of irradiated plasma, the intensity of the bands for stretching vibrations of the CH2 groups increases substantially.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 1, pp. 106–112, January–February, 2006. 相似文献
7.
E. A. Korolenko E. V. Korolik A. K. Korolik V. V. Kirkovskii 《Journal of Applied Spectroscopy》2007,74(4):561-566
We present results from an investigation of the binding ability of the main transport proteins (albumin, lipoproteins, and
α-1-acid glycoprotein) of blood plasma from patients at different stages of liver cirrhosis by the fluorescent probe method.
We used the hydrophobic fluorescent probes anionic 8-anilinonaphthalene-1-sulfonate, which interacts in blood plasma mainly
with albumin; cationic Quinaldine red, which interacts with α-1-acid glycoprotein; and neutral Nile red, which redistributes
between lipoproteins and albumin in whole blood plasma. We show that the binding ability of albumin and α-1-acid glycoprotein
to negatively charged and positively charged hydrophobic metabolites, respectively, increases in the compensation stage of
liver cirrhosis. As the pathology process deepens and transitions into the decompensation stage, the transport abilities of
albumin and α-1-acid glycoprotein decrease whereas the binding ability of lipoproteins remains high.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 74, No. 4, pp. 507–511, July–August, 2007. 相似文献
8.
G. A. Zalesskaya V. S. Ulashchik N. P. Mit’kovskaya A. V. Kuchinskii O. V. Laskina 《Journal of Applied Spectroscopy》2008,75(3):426-432
We have used the absorption spectra of whole blood, erythrocytes, and plasma to study photochemical reactions initiated by
exposure of blood in vivo to UV radiation (UV irradiation) in the UV-visible and IR regions of the spectrum. We have established
that when blood is exposed to therapeutic doses of UV radiation (0.5 J/cm2), the absorption of blood proteins decreases as monitored using the UV absorption and luminescence bands of the proteins;
photochemical reactions are initiated in the protein and heme components of the hemoglobin. For the studied doses, the reversible
reaction of photodissociation of hemoglobin complexes with oxygen is one of the most likely primary reactions initiated by
UV irradiation of blood. We conclude that changes in the position and relative intensities of the IR absorption bands of the
peptide groups (stretching and bending vibrations of NH, CN, and CO bonds) may be due to conformational transitions in the
blood protein macromolecules, induced with a change in the intermolecular hydrogen bonds on absorption of the UV radiation
by the blood. The changes in the absorption spectra of blood initiated by UV irradiation are compared with the results of
laboratory blood analyses.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 75, No. 3, pp. 400–405, May–June, 2008. 相似文献
9.
I. M. Vlasova A. Yu. Zemlyanskii A. M. Saletskii 《Journal of Applied Spectroscopy》2006,73(5):743-747
From analysis of the fluorescence spectra of eosin molecules in a solution with human serum albumin (HSA), we have obtained
information about the dynamics of protein conformational rearrangements during denaturing of the protein when treated with
sodium dodecyl sulfate (SDS) for different pH values of the solution. We hypothesize that HSA denaturing in the presence of
SDS occurs in two stages: the first stage is loosening of the protein globules, and the second stage is complete unfolding
of the protein molecules. We have shown that denaturating of the protein in the presence of SDS passes through both stages
for a solution pH below the isoelectric point of the albumin, while the denaturing stops in the first stage for a solution
pH above the isoelectric point of the albumin.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 5, pp. 661–665, September–October, 2006. 相似文献
10.
Properties of benzanthrone aminoderivative ABM (conditional name) as a potential fluorescent probe were investigated. Spectral characteristics of the compound in different solvents as well as their binding to model lipid membranes (liposomes) and human peripheral blood mononuclear cells (lymphocytes; ly) were determined. The fluorescence was found to be sensitive polarity changes to the environment. Distinctions were observed in the spectral characteristics of the investigated compound when bounded to liposomes. It was established that spectral characteristics of ABM in cell suspension qualitatively characterize the structural and functional alterations of ly during pathological phenomena and correlate directly with the clinical view of disease. The ABM is shown to be a perspective in the screening for various pathologies. 相似文献
11.
V. Yu. Plavskii V. A. Mostovnikov G. R. Mostovnikova A. I. Tret’yakova 《Journal of Applied Spectroscopy》2007,74(1):120-132
We have studied the spectral fluorescence and polarization characteristics of Z,Z-bilirubin IXα, at room temperature in chloroform
and in aqueous buffer medium, within an equilibrium complex with human serum albumin (HSA), and also under low temperature
conditions (T = −100°C) in isobutyl alcohol. We have observed a bathochromic shift of the fluorescence spectra, which is most
pronounced for the bilirubin-albumin complex. The following are considered as possible reasons for the observed dependence
of the position of the fluorescence (fluorescence excitation) spectra on the excitation (detection) wavelength: structural
and spectral differences between the chromophores making up the bilirubin molecule; conformational heterogeneity of the pigment
in solution; a contribution to the fluorescence from molecules which have not completed the vibrational relaxation process;
inhomogeneous orientational broadening of the levels; heterogeneity of the microenvironment of the chromophores in the protein
matrix. We show that polarized fluorescence of bilirubin occurs at room temperature, due to the anomalously short fluorescence
lifetime τ (picosecond or subpicosecond ranges). Despite such a short τ, the absorption and emission polarization spectra
suggest the presence of intramolecular nonradiative singlet-singlet energy transfer when bilirubin is excited to high vibrational
sublevels of the S1 state (degree of polarization p = 0.11–0.12). When fluorescence is excited on the long-wavelength slope of the absorption
band, no transfer occurs: the degree of polarization (p = 0.46−0.47) is close to the limiting value (p = 0.50). We discuss
the question of the role played by exciton interactions between chromophores in the bilirubin molecule when it is excited.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 74, No. 1, pp. 108–119, January–February, 2007. 相似文献
12.
The non-catalytic COOH-terminal module formed after proteolytic cleavage of full-length mammalian tyrosyl-tRNA synthetase displays dual function: tRNA binding ability and cytokine activity. With the aim to explore the intramolecular dynamics of C-module in solution we used fluorescence spectroscopy to study conformational changes of isolated protein. We used information from fluorescence spectra and computational model for characterization of a microenvironment of a single tryptophan residue (Trp144). Its fluorescence parameters and protection from quenching by Cs+ ions indicate the internal localization—buried into protein globule. The fluorescence quenching of Trp144 by acrylamide suggests rapid conformation dynamics of the C-module in nanosecond time scale. The temperature-induced conformational changes in the C-module were monitored by the fluorescence measurements of Trp144 emission and by red-edge excitation shift. An emission maximum shift up to ∼349 nm and significant decrease of the red-edge shift effect at 37–52 °C indicated a major conformational transition of Trp144 from buried native state into highly relaxing polar solvent environment. 相似文献
13.
14.
A. M. Saletskii A. G. Mel’nikov A. B. Pravdin V. I. Kochubei G. V. Meln’ikov 《Journal of Applied Spectroscopy》2008,75(3):402-406
We have studied the interaction between polycyclic aromatic hydrocarbons (pyrene and anthracene) with human serum albumin
(HSA) and human blood plasma. We have shown that the increase in the fluorescence intensity and the decrease in the polarity
index of pyrene on going from an aqueous solution to a pH 7.4 buffer solution of HSA suggests that polycyclic aromatic hydrocarbons
are localized in the hydrophobic microphase of the proteins. The increase in the fluorescence intensity for anthracene and
pyrene, and also the decrease in the polarity index of pyrene on going from HSA to blood plasma is connected with the fact
that polycyclic aromatic hydrocarbons can bind both to plasma proteins and to plasma lipids. When sodium dodecyl sulfate (SDS)
is added to the blood plasma in a concentration greater than the critical micelle concentration, we observe an increase in
the fluorescence intensity and the polarity index of pyrene. We hypothesize that this is connected with localization of pyrene
near the interface between the hydrophobic and hydrophilic phases of the protein-SDS system. We have established that SDS
leads to a change in the structure of blood plasma proteins and promotes escape of polycyclic aromatic hydrocarbons from the
protein globules.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 75, No. 3, pp. 379–382, May–June, 2008. 相似文献
15.
T. F. Raichenok 《Journal of Applied Spectroscopy》1999,66(3):470-472
An investigation is made of the absorption, fluorescence, and fluorescence excitation spectra of human blood serum whole and
diluted with a sodium-phosphate buffer solution. Time characteristics of the longwave serum luminescence are obtained. The
quantum yield of background fluorescence upon excitation by radiation with λexc=500 is found to be 0.5%.
Institute of Molecular and Atomic Physics of the National Academy of Sciences of Belarus, 70, F. Skorina Ave., Minsk, 220072,
Belarus. Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 66, No. 3, pp. 433–436, May–June, 1999. 相似文献
16.
T. I. Syrejshchikova Yu. A. Gryzunov N. V. Smolina A. A. Komar M. G. Uzbekov E. J. Misionzhnik N. M. Maksimova 《Laser Physics》2010,20(5):1074-1078
The efficiency of the therapy of psychiatric diseases is estimated using the fluorescence measurements of the conformational
changes of human serum albumin in the course of medical treatment. The fluorescence decay curves of the CAPIDAN probe (N-carboxyphenylimide
of the dimethylaminonaphthalic acid) in the blood serum are measured. The probe is specifically bound to the albumin drug
binding sites and exhibits fluorescence as a reporter ligand. A variation in the conformation of the albumin molecule substantially
affects the CAPIDAN fluorescence decay curve on the subnanosecond time scale. A subnanosecond pulsed laser or a Pico-Quant
LED excitation source and a fast photon detector with a time resolution of about 50 ps are used for the kinetic measurements.
The blood sera of ten patients suffering from depression and treated at the Institute of Psychiatry were preliminary clinically
tested. Blood for analysis was taken from each patient prior to the treatment and on the third week of treatment. For ten
patients, the analysis of the fluorescence decay curves of the probe in the blood serum using the three-exponential fitting
shows that the difference between the amplitudes of the decay function corresponding to the long-lived (9 ns) fluorescence
of the probe prior to and after the therapeutic procedure reliably differs from zero at a significance level of 1% (p < 0.01). 相似文献
17.
The dependence of the luminescence of the new anionic dye Pyron Red (PR) on the polarity of the medium is investigated. Upon
passage from an aqueous phase to a nonpolar phase, PR shows a shortwave shift of the fluorescence emission maximum from 675
to 650 nm and an increase in the fluorescence quantum yield from 0.03 to 0.54–0.70. When complexed with human serum albumin,
PR shows fluorescence excitation and emission maxima at 525 and 625 nm and a fluorescence quantum yield of 0.8. In a comparison
of the luminescence properties of PR with those of the well-known probes ANS and K35 in water and a complex with albumin,
PR is shown to have the maximum absolute sensitivity but a lower fluorescence enhancement upon binding with a protein compared
to ANS. A convenient criterion of the probe sensitivity toward binding with a protein that is defined as the ratio of the
fluorescence intensities of the protein-bound and the free probe AF=Fb/Ff is proposed. The value of AF(35) for the PR probe ranks between those for the K35 probe with a low AF(18) and ANS with a high AF(105).
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 66, No. 3, pp. 369–374, May–June, 1999. 相似文献
18.
N. L. Vekshin 《Journal of Applied Spectroscopy》1998,65(2):304-307
Considering the quenching of tryptophan fluorescence of bovine serum albumin by various dyes as an example, it is shown that
overlap of radiation and absorption spectra does not necessarily lead to energy transfer by resonance. No correlation is revealed
between the limiting quenching and the Forster overlap integral. Quenching can occur even in the absence of overlap. The magnitude
of energy transfer is markedly lower than that of quenching owing to competing processes, namely, excitation deactivation
by the dye and, probably, by the protein itself which undergoes conformation upon sorption of the dye. Negatively charged
and neutral dyes posses, on the average, a higher quenching activity relative to albumin than do positively charged dyes.
Institute of the Biophysics of Cells of the Russian Academy of Sciences, Pushchino, 142292, Moscow Region, Russia. Translated
from Zhurnal Prikladnoi Spektroskopii, Vol. 65, No. 2, pp. 290–293, March–April, 1998. 相似文献
19.
We have obtained and analyzed the absorption, fluorescence, and fluorescence excitation spectra of indole vapor, N-acetyl-L-tryptophan
vapor, and 3-indole aldehyde vapor. From analysis of the dependence of the fluorescence spectrum of the free indole molecules
on the wavelength of the exciting radiation λex, it follows that emission of fluorescence occurs when the molecules undergo a transition from the one electronically excited
state 1Lb. The fluorescence spectra of the studied compounds are insignificantly different, suggesting a major role for the indole
chromophore in formation of the compounds. The absorption spectrum of N-acetyl-L-tryptophan, in which the group of atoms is
added to the indole ring through a-C-C bond, is similar to the spectrum of indole, while the spectrum of 3-indole aldehyde
is significantly different from the indole spectrum due to the effect of the C=O group conjugated with the indole ring. The
fluorescence excitation spectra are considerably different from the absorption spectra. This is associated with the strong
dependence of the quantum yield for the free molecules on λex. Qualitatively, they are mirror-symmetric to the fluorescence spectra of the stodied compounds. Analysis of the data obtained
provides a basis for assuming that in the case of free molecules of indole and its derivatives, the 1La absorption in the extreme long-wavelength region of the spectrum does not overlap 1Lb absorption.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 74, No. 2, pp. 218–222, March–April, 2007. 相似文献
20.
N.?A.?Nemkovich Yu.?V.?Kruchenok A.?N.?Sobchuk H.?Detert N.?Wrobel E.?A.?Chernyavski? 《Optics and Spectroscopy》2009,107(2):275-281
The spectral and temporal characteristics of new 6,12-dimethoxyindolo[3,2-b]carbazole, 5,11-dimethyl-6,12-dimethoxyindolo[3,2-b]carbazole, and 5,11-dihexyl-6,12-di(hexyloxy)indolo[3,2-b]carbazole fluorescence probes in organic solvents and protein complexes are studied. The dipole moments of indolocarbazoles
in 1,4-dioxane were measured by electrooptical absorption method. The measured dipole moments have values within the range
of (3.1–3.6) × 10−30 C m in the equilibrium ground state and increase to (4.8–5.6) × 10−30 C m after excitation. The excited state lifetime of indolocarbazole derivatives increases with increasing polarity and viscosity
of the environment. The binding of indolocarbazoles with trypsinogen and human serum albumin increases the fluorescence intensity,
changes the intensity ratio of fluorescence bands, and increases the average excited state lifetime of indolocarbazoles. The
analysis of the instantaneous fluorescence spectra and fluorescence decay parameters at different wavelengths revealed the
existence of several types of probe binding sites in proteins. It is found that the fluorescence characteristics of indolocarbazole
derivatives depend on the conformation rearrangements of trypsinogen due to its thermal denaturation. 相似文献