Investigation of the protein component of the lipopolysaccharide-protein complex ofYersinia pseudotuberculosis

It has been established by electrophoresis in polyacrylamide gel that the protein component of the lipopolysaccharide-protein complex ofYersinia pseudotuberculosis consists of two poly-peptides with molecular weights of 42,000–45,000 and 18,000–20,000. These polypeptides have been detected in the cell wall ofY. pseudotuberculosis as the main components. Protein fractions B-2 and B-3, differing with respect to solubility in phenol, were isolated from the LPPC ofY. pseudotuberculosis by extraction with hot 45% phenol. These protein fractions had the same polypeptide composition, which is characteristic for membrane proteins. B-3 included the LPS component and B-2 contained lipid A or fragments of it as impurity. Fractions B-3 and B-2 precipitated with antiserum to the LPPC.Pacific Ocean Institute of Bioorganic Chemistry of the Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 92–97, January–February, 1980.     

Characteristics of the molecular-mass parameters and viscosities of solutions of the lipopolysaccharide-protein complex fromYersinia pseudotuberculosis

The lipopolysaccharide-protein complex (LPPC) fromY. pseudotuberculosis isolated by extraction with trichloroacetic acid has been investigated by the methods of gel permeation chromatography, light scattering, and viscometry. The molecular-mass distributions of aggregates of the LPPC in water, 0.03 M Tris-HCl buffer (pH 8) and 0.1 M sodium chloride have been determined. A dependence of the polydispersity and dimensions of the aggregates on the concentration of the polymer and on the ionic strength of the solvent has been shown. It has been established that in water the LPPC has a high characteristic viscosity which falls with an increase in the ionic strength of the solution.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostock. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 384–388, May–June, 1982.     

Isolation,purification, and some physicochemical properties of glucose isomerase from Streptomyces atratus

A highly purified glucose isomerase with a specific activity 23.5 times greater than that of a homogenate of the mycelium has been obtained fromStreptomyces atratus by methods of homogenization from the freeze-dried biomass, heat treatment (70°C, 10 min), ion-exchange chromatography, and gel filtration. The yield of enzyme on the initial biomass was 51.8%. The molecular mass of the enzyme has been determined by various methods as 160 kDa but in the presence of sodium dodecyl sulfate in thin-layer PAG it amounts to 40 kDa.Institute of Microbiology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 524–528, July–August, 1990.     

Histones of the cotton plant. Molecular characteristics of histone H1

A characterization of the histones of two varieties of the cotton plant,Gossypium hirsutum andG. barbadense, has been given with the aid of various electrophoretic systems. Their molecular weights have been determined. An analysis has been made of the cleavage of histone H1 at tyrosine residues. The positive charge and the molecular length of histone H1 have been determined by the method of incomplete succinylation.V. I. Nikitin Institute of Chemistry, Academy of Sciences of the Tadzhik SSR, Dushanbe. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 491–497, July–August, 1986.     

Phospholipase A2 from the venom of the spider Eresus niger

A phospholipase A₂ with a molecular mass of 12–14 kDa has been isolated from the venom of the spiderEresus niger by three-stage column chromatography. Asparagine has been identified as the N-terminal amino acid.Institute of Physiology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 557–560, July–August, 1991.     

Analysis of the nerve growth activities of protein fractions from the venoms of Agkistrodon halys halys and Echis multisquamatus

The possibility has been shown of obtaining NGFs in the process of isolating coagulant proteinases and other biologically active substances from the venoms of theAgkistrodon halys halys and theEchis multisquamata. Results characterizing the NGFs of both species as basic (weakly basic) proteins with molecular masses of 30.0–40.0 kDa are presented.Institute of Biochemistry, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 266–270, March-April, 1992.     

Phospholipids of the seeds of two species of Erysimum

Summary The phospholipid complexes of the seeds ofErysimum diffusum andE. sylvestris have been investigated. It has been found that the amount of phospholipids in the seeds ofE. diffusum is lower than in those ofE. sylvestris, but their qualitative compositions are similar. The fatty-acid compositions of the triglycerides and of the total phospholipids of the two plants investigated have been studied. It has been established that the total phospholipids of the species ofErysimum under consideration and individual fractions of them differ by the degree of saturation and the qualitative and quantitative composition of the acids that they contain and, consequently, the phospholipids present in greatest amount differ in their molecular compositions.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 623–627, September–October, 1977.     

Glutamate receptor blockers from venoms of the spiders Argiope lobata and Araneus tartaricus

The presence of glutamate receptor blockers with molecular masses below 1000 Da and pI9 has been detected in the venoms of the spidersArgiope lobata andAraneus tartaricus.Institute of Physiology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 532–537, July–August, 1990.     

Isolation of an intracellular lipase from the heat-tolerant fungus Rhizopus microsporus UzLT-1 and its properties

Summary 1. A method of isolating and purifying an intracellular lipase of the fungusRhizopus microsporus, UzLT-1, has been described.2. Some properties of the purified enzyme have been studied and it has been shown that the intracellular lipase differs from the extracellular lipase in its pH optimum and electrophoretic mobility.3. The presence of sodium chloride (0.06 M) in the reaction mixture raises the heat stability of the purified lipase.Division of Microbiology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 566–570, July–August, 1977.     

Competitive bindings of histones with DNA in formation of plant nucleohistones

An acid form of ricin — ricin T — with a molecular weight of 58 kDa, an isoelectric point, pI, of 7.0–7.1, and a sedimentation coefficient of 4.60 S has been isolated from the seeds of the Central Asian castor-oil plantRicinus communis by gel filtration on Sephadex G-75 followed by ion exchange chromatography on DEAE- and CM-celluloses. The amino acid composition and the N-terminal amino acid residues of ricin T have been determined.Institute of the Chemistry of Plant Substances, Uzbek Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 883–887, November–December, 1987.     

Structure of isobadkhysin. The stereochemistry of badkhysin and isobadkhysin

Conclusions 1. By chromatography of the total lactones obtained from the sodium carbonate treatment of the resin of the roots ofFerula oopoda (Boiss. et Buhse) Boiss. we isolated a previously unstudied sesquiterpene lactone C₂₀H₂₄O₅, mp 189–190° C, which we called isobadkhysin.2. It has been shown that isobadkhysin is a stereoisomer of badkhysin and is formed during the treatment of the resin with sodium carbonate.3. The stereochemical characteristics of badkhysin and isobadkhysin have been established on the basis of their NMR spectra.Khimiya Prirodnykh Soedinenii, Vol. 6, No. 4, pp. 425–428, 1970     

Crystal and molecular structures of 1,8-diacetyl-3-methyl-DL-allantoin

DL-Allantoin (I) has been isolated from an alcoholic extract of a marine spongeAxinellidae gen. sp., and derivatives of it have been obtained: 1, 8-diacetyl-DL-allantoin (II) and 1,8-diacetyl-3-methyl-DL-allantoin (III). The crystal and molecular structures of (III) have been established by X-ray structural analysis.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, USSR Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 422–426, May–June, 1988.     

Molecular composition of the N-acylphosphatidylethanolamine of the cotton plant of variety S-6029

An N-arylphosphatidylethanolamine has been isolated from the total phospholipids of the cotton plant of variety S-6029. The composition and the position distribution of the fatty-acid radicals has been studied with the aid of enzymatic hydrolysis with phospholipase A₁ (from the fungusRhisophus microsporus UzLT-1) and by alkaline and acid hydrolyses. From the results obtained, the possible molecular composition of the N-acylphosphatidylethanolamine has been calculated by Coleman's method. The total number of all the molecular species amounted to 720.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 780–783, November–December, 1979.     

Characteristics of the glucose isomerase from Streptomyces atratus

The molecular mass (Mr) of highly purified glucose isomerase fromStreptomyces atratus has been determined. The native glucose isomerase, which has affinity for xylose, consists of an oligomeric protein containing 1552 amino acid residues and formed of four subunits. The enzyme is stable in the range of temperatures from 40 to 80°C and at pH values of from 6.0 to 11.0.Institute of Microbiology, Uzbek Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 833–837, November–December, 1991.     

Spatial structure of the dimeric sesquiterpene germacrane lactone mikagoyanolide

The dimeric sesquiterpene lactone mikagoyanolide (I) has been isolated from the plantTanacetopsis mucronata. The spatial structure of (I) has been determined by the x-ray structural method (R=0.086, 1161 reflections). The dimeric lactone is formed from cyclodecadienes of the germacrolide and melampolide types having chair-chair and boat-chair conformations. The conformational state of the free molecule of (I) has been calculated by the method of molecular mechanics.Institute of Chemistry of Plant Substances, Uzbekistan Republic of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 213–217, March–April, 1993.     

A spectroscopic investigation of the phenolcarboxylic acids ofCynara scolymus

Summary Spectroscopic investigations in the UV region of the phenolcarboxylic acids that we have isolated from the leaves ofCynara scolymus have been carried out. It has been found that:1. Free caffeic acid can be distinguished from its esters by the changes in the UV spectra under the influence of sodium ethoxide and sodium acetate.2. An o-dihydroxy grouping in esters can be detected from the bathochromic shift observed with boric acid in the presence of sodium acetate.3. An o-dihydroxy grouping can be detected both in esters and in the free acid under the influence of aluminum chloride.Khimiya Prirodynkh Soedinenii, Vol. 2, No. 5, pp. 303–306, 1966     

Esters of Ferula karakalensis. Structure and stereochemistry of karaferin and karaferinin

Two new guaiane esters — karaferin and karaferinin — have been isolated fromFerula karakalensis, and their structures and stereochemistry have been established. It has been shown that theFerula sesquiterpene alcohols angrendiol, ugamdiol, and karaferol are biogenetically interconnected and are products of a single chain of the biogenesis of terpenoids in plants.Institute of Chemistry of Plant Substances, Uzbekistan Republic Academy of Sciences, Tashkent; Botanical Garden of Moscow State University. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 227–231, March–April, 1993.     

Steroid compounds from ophiuroids. I. New steroid sulfate from Ophiura sarsi

The sulfate of a steroid polyol has been isolated from the Far Eastern ophiuroidOphiura sarsi, and its structure has been established by chemical and spectral methods as cholest-5-ene-3,4,21-diol 3-(sodium sulfate). No steroid compounds of such structure from ophiuroids have been described previously.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, USSR Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 375–379, May–June, 1988.