UPLC－ESI－Q－TOF MS结合裂解规律分析姜科植物中二苯基庚烷类成分

根据标准品的质谱数据,探讨二苯基庚烷类成分的裂解规律,并利用裂解规律分析姜科植物中的该类成分。采用针泵注射进样,利用高分辨率和高准确性的电喷雾－四极杆－飞行时间质谱（ESI－Q－TOF MS）得到正离子模式下（ESI+）11个标准品的质谱数据。在此基础上,分析标准品的裂解行为,归纳4类二苯基庚烷类化合物的裂解途径,总结出二苯基庚烷类化合物的裂解规律。据此建立快速识别二苯基庚烷类成分的方法,结合超高效液相色谱－电喷雾－四极杆－飞行时间串联质谱（UPLC－ESI－Q－TOF MS）,对姜科植物来源中药姜皮、草果、益智仁中的二苯基庚烷类成分进行识别,从中分别鉴定出14、11、10个此类成分。结果表明,该方法准确、可靠、高效,可快速鉴定和分析姜科植物中的二苯基庚烷类化合物。     

烷基苯磺酸盐的电喷雾离子化质谱和碰撞活化解离质谱－质谱分析

用正,负电喷雾电离（ESI）并结合碰撞活化解离（CAD）质谱法对烷基苯磺酸盐（ABS）进行鉴定,无论正,负离子化过程中均不出现快原子轰击质谱常见的碎片峰,由于没有复杂碎片峰的干扰,ESI－MS对分析ABS试样大为有利,正离子ESI－MS对支化ABS鉴定的灵敏度远低于负离子ESI－MS,用CAD－MS对ESI－ME谱各主要峰进行了归属,线型与支化ABS相对含量可以用负离子ESI－MS求出,负离子化ESI－MS是快速,有效和可靠的鉴定,ABS的方法。     

固相微萃取气质联用技术对卷烟烟丝和卷烟烟气总粒相物中挥发性和半挥发性成分的研究

采用固相微萃取－气相色谱－质谱（SPME－GC－MS）法对成品卷烟烟丝和卷烟烟气总粒相物中挥发性和半挥发性成分进行了分析鉴定，并对鉴定出的70种成分和96种成分进行了比较。     

气相色谱-质谱联用分析白兰叶油成分

采用气相色谱－质谱联用技术对苏州产白兰叶油的化学成分进行了分析,分离出３３个峰。鉴定了其中２７种成分,占总峰面积的９７％,并对油中的主要化学成分芳樟醇用气相色谱－傅立叶红外光谱法进行了验证。     

聚芴类发光材料合成中间体的质谱分析

利用电喷雾电离（ESI）质谱－质谱（MS－MS）技术，分析了新化合物2，7－双（4，4，5，5－四甲基－1，3，2－二氧杂硼烷－2－基）－9，9－二辛基芴的EI和ESI的质谱，确认了该谱图中母离子和子离子的关系，讨论了其碎裂途径，为其结构鉴定提供了依据。     

基于UFLC－IT－TOF MS分析技术的妇科再造丸化学成分研究

大品种妇科再造丸由42味中药组成,对多种妇科疾病具有显著疗效。但其组方复杂,化学成分尚未被充分阐明,质量控制水平较低。该研究建立了一种采用超快速液相色谱－离子阱飞行时间质谱联用技术（UFLC－IT－TOF MS）快速鉴定妇科再造丸化学成分的方法,系统研究了其化学物质基础。采用COSMOSIL C18色谱柱（3.0 mm × 150 mm,2.6 μm）进行分离,以0.4%乙酸和乙腈为流动相梯度洗脱;采用电喷雾离子源（ESI）在正负离子模式下采集妇科再造丸MS1、MS2和MS3的碎片信息。通过推导质谱裂解规律,与对照品、文献数据和单味药材图谱比对等方式,共鉴定出155种化学成分,包括萜类成分47个,酚及酚酸类成分43个,黄酮类成分39个,生物碱类成分14个,苯酞类成分8个,其他类成分4个,其中133个成分首次在妇科再造丸中发现。该研究进一步阐明了妇科再造丸的化学成分组成,为其质量标准的提高及药效物质的阐明提供了丰富信息。     

气相色谱-质谱法测定姜黄挥发油化学成分

采用水蒸气蒸馏法提取姜黄挥发油,用气相色谱－质谱联用技术分离和鉴定姜黄挥发油的化学成分。经计算机ＮＢＳ谱库检索,发现姜黄挥发油中至少有１５个峰,鉴定出α－姜黄烯、α－姜烯、桉叶油素和球姜酮等１５种组分;另外还有１－（３－环戊基醛）－２,４－二甲基苯、β－倍半水芹烯、大根香叶酮、大根香叶烷、顺双环［３,３,１］酮－２－烯－９－醇等成分。姜黄挥发油中主要成分为α－姜黄烯。     

基于UHPLC－Q－Exactive－MS技术的短序蒲桃叶岩白菜素新衍生物的快速发现

基于课题组前期对短序蒲桃叶的研究,该文建立了快速鉴定短序蒲桃叶中未知岩白菜素类化学成分的超高效液相色谱－串联四极杆轨道阱质谱（UHPLC－Q－Exactive－MS）分析方法。采用Waters ACQUITY UPLC HSS T3色谱柱（1.8 μm,2.1 mm × 100 mm）,乙腈－0.1%甲酸水为流动相梯度洗脱,Orbitrap MS负离子模式下检测。总结了模式成分的质谱裂解规律和特征碎片离子,并采集高分辨质谱数据对短序蒲桃叶中的岩白菜素衍生物等酚酸类成分进行鉴定。从短序蒲桃叶中鉴定出54种酚酸类成分,包含34种岩白菜素衍生物,均为在蒲桃属植物中首次报道,其中发现潜在的新岩白菜素类化合物10个。所得结果为进一步研究和利用民族中草药短序蒲桃提供了参考。     

新型抗炎镇痛剂SFZ-47及其代谢物的电喷雾离子阱质谱研究

用电喷雾离子阱质谱对警犬尿样中SFZ－47[3H-1,2-二氢－2－（4－甲基苯胺基）甲基－1－吡咯里嗪酮）及其4种代谢物进行了结构鉴定，利用质谱解析软件分析其裂解方式发现，它们在（＋）ESI－MS^2或（ )ESI－MS^3质谱中分别生成m/z122和脱吡咯里嗪酮母核的碎片，并发现葡萄苷酸型代谢物易于生成脱水（18u)和脱葡萄醛酸（176u)的碎片离子，这些特征可用于SFZ－47及结构类似物的体内生物转化研究。     

香椿子油成分的 GC-MS分析

用正己烷抽提得香棒子,然后将样品进行皂化、甲酯化,以毛细管色谱－质谱联用法（GC－MS）测定了香棒子油的组成,共鉴定出39种成分,其中亚油酸相对含量为83．45％。     

Furyl- and thienyl-silatranes and germatranes

We review our research on the synthesis and study of the physical and biological properties of furyl- and thienylgermatranes and -silatranes.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 6, pp. 725–732, June, 1992.     

Review and patents and literature

The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed of genetic engineering, (3) ability to accommodate large DNA inserts, (4) protein processing similar to higher eukaryotic cells (e.g., mammalian cells), and (5) ease of insect cell growth (e.g., suspension growth). The following review of the literature discusses two engineering aspects of recombinant protein synthesis by insect cell cultures: bioreactor scale-up and insect cell line selection. Following this review patent abstracts and additional literature pertaining to expression of recombinant proteins in insect cell culture are listed.     

多环芳二酐型聚酯亚胺膜的透气性能

多环芳二酐型聚酯亚胺膜的透气性能李悦生，丁孟贤，徐纪平（浙江大学高分子科学与工程研究所，杭州，３１００２７）（中国科学院长春应用化学研究所）关键词聚醚酰亚胺，聚酯酰亚胺，膜，透气性通常的聚酰亚胺加工性能较差，在芳环二酐的苯环间引入醚键等柔性基团后，其...     

微量元素与疾病诊断治疗的研究现状及展望

对国内外微量元素与疾病的关系及其诊断治疗的研究现状及研究动向进行了综述，包括：微量元素与地方病、癌肿、心血管病、糖尿病、眼病、神经系统病等。参考文献72篇。     

Preparation and photolysis of poly-p-ethylacrylophenone and poly-p-chloroacrylophenone and their copolymers with styrene and methyl methacrylate

Poly-p-ethylacrylophenone (PPEtAP) and poly-p-chloroacrylophenone (PPClAP) and their copolymers with styrene (PEtAP/S) and (PClAP/S) and with methyl methacrylate (PEtAP/MMA) and (PClAP/MMA) were prepared. Quantum yields of main-chain scissions at 366 nm at room temperature in benzene solution are of the same order as those of the unsubstituted polyacrylophenone and its copolymers. Substitution with chlorine and ethyl group in the para position compared to the unsubstituted polyacrylophenone and its copolymers leads to an increase of the lifetime for the n–π^* excited triplet state, as is evident from the measured quenching constants of photolysis. The low-temperature emission spectra of the copolymers are similar to the spectra of the model compounds in a polymer film. For the homopolymer, however, the character of the emission spectrum changes considerably.     

Concentrations and distributions of trace and minor elements in Chinese and Canadian coals and ashes

A total of 35 trace and minor elements including some of environmental significance were determined in each of a selection of 15 Chinese and 6 Canadian thermal coals and their ashes by using the SLOWPOKE-2 nuclear reactor facility of the University of Toronto. The concentrations and distributions of these constituents among the coals and their combustion products (viz. ash and volatile matter) are presented together with an interpretation of their significance in relation to the large scale combustion of these coals as thermal fuels in industrialized countries such as China and Canada. The detailed results showed wide variations in trace impurity concentrations (up to a factor of 100 and more) among the coals studied with few large differences between those of Chinese and Canadian origin except that the rare earths, Sc, Th, U, I, and Se were much higher in the former, other halogens, As and Na were lower. Values for elemental enrichment factors (EF) relative to normal crustal abundances indicated that only As(EF=13), Br(5.7), I(16), S(230), Sb(11) and Se(320) were appreciably enriched in coal. During static ashing at 750°C most of the halogens, S and Se were volatilized whereas most other inorganic constituents were highly retained and concentrated in the ash by factors of 6 to 11.     

Ground- and excited-state aromaticity and antiaromaticity in benzene and cyclobutadiene

The aromaticity and antiaromaticity of the ground state (S 0), lowest triplet state (T 1), and first singlet excited state (S 1) of benzene, and the ground states (S 0), lowest triplet states (T 1), and the first and second singlet excited states (S 1 and S 2) of square and rectangular cyclobutadiene are assessed using various magnetic criteria including nucleus-independent chemical shifts (NICS), proton shieldings, and magnetic susceptibilities calculated using complete-active-space self-consistent field (CASSCF) wave functions constructed from gauge-including atomic orbitals (GIAOs). These magnetic criteria strongly suggest that, in contrast to the well-known aromaticity of the S 0 state of benzene, the T 1 and S 1 states of this molecule are antiaromatic. In square cyclobutadiene, which is shown to be considerably more antiaromatic than rectangular cyclobutadiene, the magnetic properties of the T 1 and S 1 states allow these to be classified as aromatic. According to the computed magnetic criteria, the T 1 state of rectangular cyclobutadiene is still aromatic, but the S 1 state is antiaromatic, just as the S 2 state of square cyclobutadiene; the S 2 state of rectangular cyclobutadiene is nonaromatic. The results demonstrate that the well-known "triplet aromaticity" of cyclic conjugated hydrocarbons represents a particular case of a broader concept of excited-state aromaticity and antiaromaticity. It is shown that while electronic excitation may lead to increased nuclear shieldings in certain low-lying electronic states, in general its main effect can be expected to be nuclear deshielding, which can be substantial for heavier nuclei.