共查询到18条相似文献,搜索用时 125 毫秒
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简单介绍了毛细管电泳在医药领域中的应用,包括药物分析、中药成分分析、手性对映体分离分析和临床化学、法医及单细胞分析等。药物分析中包括主药成分分析、相关杂质检测、药物计量离子配比测定和定量分析等。中药成分分析中包括各类药效成分、中药材中主要成分及中药复方制剂成分分析。手性对映体分离中包括机理研究、新型手性选择剂。临床化学中包括临床疾病诊断、临床蛋白分析、临床药物监测、药物代谢研究和分子生物学测定。法庭科学中包括毒物分析、枪击残余物分析、炸药分析、笔迹墨水分析。单细胞分析中包括神经细胞分析、红细胞分析和胚胎细胞分析。 相似文献
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毛细管电泳的原理及应用(第四讲):毛细管电泳在氨基酸、肽及蛋白分析中的应用 总被引:5,自引:0,他引:5
毛细管电泳的原理及应用(第四讲)毛细管电泳在氨基酸、肽及蛋白分析中的应用罗国安王义明周国华(清华大学化学系北京100084)1概述毛细管电泳(CE)的应用包括很多内容。被分析的物质有离子、小分子、生物大分子乃至高分子和粒子的分析;分析对象从无机物、有机物、生物体系乃至活体单个细胞的分析;含量测定范围可从常量到微量乃至几个分子的测定。本文只介绍应用最多的几方面内容,其中首推多肽和蛋白的分析,可以说它是CE最初的应用之一。 相似文献
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毛细管电泳的原理及应用(第二讲)毛细管电泳的原理及应用罗国安,王义明(清华大学生命科学与工程研究院,清华大学化学系北京100084)1概述毛细管电泳(CE)除了比其它色谱分离分析方法具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点外,... 相似文献
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毛细管电泳的原理及应用(第二讲)毛细管电泳的原理及应用 总被引:17,自引:0,他引:17
毛细管电泳的原理及应用(第二讲)毛细管电泳的原理及应用罗国安,王义明(清华大学生命科学与工程研究院,清华大学化学系北京100084)1概述毛细管电泳(CE)除了比其它色谱分离分析方法具有效率更高、速度更快、样品和试剂耗量更少、应用面同样广泛等优点外,其仪器结构也比高效液相色谱(HPLC)简单。CE只需高压直流电源、进样装置、毛细管和检测器。前三个部件均易实现,困难之处在于检测器。 相似文献
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毛细管电泳的原理及应用(第三讲)毛细管电泳的柱技术 总被引:1,自引:0,他引:1
毛细管电泳的原理及应用(第三讲)毛细管电泳的柱技术王义明,罗国安(清华大学化学系,清华大学生命科学与工程研究院北京100084)关键词毛细管电泳,柱技术1概述毛细管电泳(CE)的分离和检测过程均在毛细管内完成,所以说毛细管是CE的核心部件之一。早期对毛细管的研究集中在毛细管直径、长度、形状和材料方面。采用细柱可减少电流及自热,而且能加快散热,以保持高效分离;但会造成进样、检测及清洗上的困难,也不利于对吸附的抑制,故现在一般采用25~100μm内径的毛细管。 相似文献
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高效毛细管电泳在药物分析中的应用 总被引:10,自引:1,他引:10
对高效毛细管电泳(HPCE)在药物制剂及原料药分析中的应用(测定药物中主成分及杂质含量;手性药物拆分;微量制备及成分鉴别等)作一综述,并用表1,2列出了部分应用实例。 相似文献
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Mengyao Wang Qian Gong Wenfang Liu Songwen Tan Jian Xiao Chuanpin Chen 《Journal of separation science》2022,45(11):1918-1941
So far, the potential of capillary electrophoresis in the application fields has been increasingly excavated due to the advantages of simple operation, short analysis time, high-resolution, less sample consumption, and low cost. This review examines the implementations and advancements of capillary electrophoresis in different application fields (environmental, pharmaceutical, clinical, and food analysis) covering the literature from 2019 to 2021. In addition, ultrasmall sample injection volume (nanoliter range) and short optical path lead to relatively low concentration sensitivity of the most frequently used ultraviolet-absorption spectrophotometric detection, so the pretreatment technology being developed has been gradually utilized to overcome this problem. Despite the review being focused on the development of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis, the new sample pretreatment techniques of microextraction and enrichment fit excellently to capillary electrophoresis in recent three years are also described briefly. 相似文献
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This review presents different solvents and electrolytes commonly used as BGEs in NACE for the analysis of pharmaceutical compounds. Most NACE applications carried out since 1998 for the analysis of compounds of pharmaceutical interest are presented in four tables: (i) analysis of drugs and related substances, (ii) analysis of chiral substances, (iii) analysis of phytochemical extracts and (iv) analysis of drugs in biological fluids. These selected examples are used to illustrate the interest in NACE versus conventional aqueous CE. 相似文献
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Leena Suntornsuk 《Analytical and bioanalytical chemistry》2010,398(1):29-52
This review covers recent advances of capillary electrophoresis (CE) in pharmaceutical analysis. The principle, instrumentation,
and conventional modes of CE are briefly discussed. Advances in the different CE techniques (non-aqueous CE, microemulsion
electrokinetic chromatography, capillary isotachophoresis, capillary electrochromatography, and immunoaffinity CE), detection
techniques (mass spectrometry, light-emitting diode, fluorescence, chemiluminescence, and contactless conductivity), on-line
sample pretreatment (flow injection) and chiral separation are described. Applications of CE to assay of active pharmaceutical
ingredients (APIs), drug impurity testing, chiral drug separation, and determination of APIs in biological fluids published
from 2008 to 2009 are tabulated. 相似文献
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毛细管区带电泳法快速测定复方布洛芬片的有效成分 总被引:3,自引:0,他引:3
研究了用毛细管区带电泳法快速测定复方布洛芬片中布洛芬和伪麻黄碱含量的方法。在0.025mol/L的磷酸盐缓冲液(pH8.1)中,上述两组分可在3min内得以完全分离,用紫外检测器在210nm处检测,并以外标法定量。11次测定含有9.5mg/L盐酸伪麻黄碱和66.7mg/L布洛芬的试样溶液,相对标准偏差为2.9%(伪麻黄碱)和1.9%(布洛芬),回收率为103.1%(伪麻黄碱)和97.6%(布洛芬)。应用毛细管区带电泳法测定复方布洛芬片剂的含量,所得结果与HPLC法一致。 相似文献
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Recent developments in chiral capillary electrophoresis and applications of this technique to pharmaceutical and biomedical analysis 总被引:2,自引:0,他引:2
Amini A 《Electrophoresis》2001,22(15):3107-3130
This paper provides an overview of the current status of chiral capillary electrophoresis (CE). The emphasis is placed on the application of CE in chiral separation of various racemic compounds. During the last two years about 280 papers, several review articles, and two entire issues, edited by S. Fanali (Electrophoresis 1999, 20, 2577-2798, and H. Nishi and S. Terabe (J. Chromatogr. A 2000, 879, 1-471.) have been devoted to chiral CE. Enantiomeric separations of various compounds, e.g., pharmaceuticals, drug candidates, drugs and related metabolites in biological fluids, amino acids, di- and tri peptides, pesticides and fungicides, have been performed using different chiral selectors. Native and derivatized cyclodextrins continue to be the most widely used chiral selectors. Other chiral selectors such as natural and synthetic chiral micelles, crown ethers, chiral ligands, proteins, oligo- and polysaccharides, and macrocyclic antibiotics have also been applied to chiral CE separations. 相似文献