共查询到20条相似文献,搜索用时 109 毫秒
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从80年代初交联毛细管柱和大内径厚液膜毛细管柱的问世,显示了毛细管色谱法的突出优点,从而以毛细管柱逐步取代填充柱成为必然趋势。气相色谱法从1959年开始用于火炸药分析,从80年代初起把毛细管气相色谱用于火炸药污染物的分析以来,气相色谱法已成为火炸药分析十分有用的工具。根据我们多年的研究认为毛细管气相色谱法在很多情况下优于填充柱气相色谱法。 相似文献
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《液相色谱法及相关技术杂志》2012,35(9):1705-1716
Abstract No single isocratic chromatographic technique allows the complete separation of common organic aliphatic and alicyclic acids of plants. In order to obtain a better isocratic separation with a single HPLC method we combined in one chromatography assay the Ion Exchange and Reverse Phase technics in building a twin phase column. The first attempts are promising. This double chromatography based on the polarity of molecule (Reverse Phase) and on its acidic characteristics (Ion Exchange) has the advantages of both methods and allows good separations of the acids. 相似文献
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HAN Jing WU Xi CAI Wensheng SHAO Xueguang 《高等学校化学研究》2014,30(4):578-581
Ginseng is one of the most important traditional Chinese medicines and functional foods.A method for the fast determination of amino acids in ginseng samples using high performance liquid chromatography(HPLC) was developed,in which strong isocratic elution was employed for simplifying the separation and speeding up the analysis.All amino acids were eluted within 3 min with the chromatogram composed of overlapped peaks from the interferences.Then,non-negative immune algorithm(NNIA) was adopted to resolve the chromatographic signals of the components from the chromatogram measured.The results show that the signals of the amino acids can be correctly extracted by NNIA and the signal extracted can be used for the quantitative analysis.The method was validated via determining six amino acids of four different samples of ginseng.The recoveries of the spiked samples are in a range of 96.6%-106.3%. 相似文献
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Stella S. Brudin Robert A. Shellie Paul R. Haddad Peter J. Schoenmakers 《Journal of chromatography. A》2010,1217(43):6742-6746
In the work presented here a novel approach to comprehensive two-dimensional liquid chromatography is evaluated. Ion chromatography is chosen for the first-dimension separation and reversed-phase liquid chromatography is chosen for the second-dimension separation mode. The coupling of these modes is made possible by neutralising the first-dimension effluent, containing KOH, prior to transfer to the second-dimension reversed-phase column. A test mixture of 24 low-molar-mass organic acids is used for optimisation of the system. Three food and beverage samples were analysed in order to evaluate the developed methodology, the resulting two-dimensional separation is near-orthogonal, the set-up is simple and all instrumental components are available commercially. The method proved to be robust and suitable for the analysis of wine, orange juice and yogurt. 相似文献
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Summary This report describes a modified method for the separation and analysis of polyunsaturated fatty acids such as 20:4, 20:5,
and 22:6, using HPLC. The results show that these fatty acids are well separated from the saturated acids. Since the unsaturated
fatty acids elute earlier than saturated acids, and this method does not require the fractionation of free fatty acids using
thin layer chromatography, a necessary step for the gas chromatographic analysis, the recoveries of polyunsaturated fatty
acids were significantly higher as compared to those from gas chromatography. Furthermore, HPLC and gas chromatographic methods
gave identical results for the acyl chain composition of phosphatidylserine. The advantages of using HPLC over gas chromatography
in determining the acyl chain composition of free fatty acids and phospholipids are also discussed. 相似文献
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氨基酸的分析方法及其应用进展 总被引:35,自引:0,他引:35
从衍生试剂角度,介绍了不同衍生化氨基酸的分析方法,包括离子交换色谱法、高效液相色谱法、气相谱法和毛细管电泳法,以及无需衍生化的直接分析法高效阴离子交换色谱-积分脉冲安培法,并总结了蛋白质、食品和生理体液样品中的氨基酸分析方法。 相似文献
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Amino acids in foods exist in a free form or bound in peptides, proteins, or nonpeptide bonded polymers. Naturally occurring L-amino acids are required for protein synthesis and are precursors for essential molecules, such as co-enzymes and nucleic acids. Nonprotein amino acids may also occur in animal tissues as metabolic intermediates or have other important functions. The development of bacterially derived food proteins, genetically modified foods, and new methods of food processing; the production of amino acids for food fortification; and the introduction of new plant food sources have meant that protein amino acids and amino acid enantiomers in foods can have both nutritional and safety implications for humans. There is, therefore, a need for the rapid and accurate determination of amino acids in foods. Determination of the total amino acid content of foods requires protein hydrolysis by various means that must take into account variations in stability of individual amino acids and resistance of different peptide bonds to the hydrolysis procedures. Modern methods for separation and quantitation of free amino acids either before or after protein hydrolysis include ion exchange chromatography, high performance liquid chromatography (LC), gas chromatography, and capillary electrophoresis. Chemical derivatization of amino acids may be required to change them into forms amenable to separation by the various chromatographic methods or to create derivatives with properties, such as fluorescence, that improve their detection. Official methods for hydrolysis and analysis of amino acids in foods for nutritional purposes have been established. LC is currently the most widely used analytical technique, although there is a need for collaborative testing of methods available. Newer developments in chromatographic methodology and detector technology have reduced sample and reagent requirements and improved identification, resolution, and sensitivity of amino acid analyses of food samples. 相似文献
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金属络合物高效液相色谱—光度分析进展 总被引:2,自引:0,他引:2
本文评述了金属络合物高效液相色谱分析近期进展,包括了基本方法,色谱及光度检测条件。在有机相、水相和胶束相中形成的络合物可直接注入到色谱系统,或是将金属离子溶液注入到含有络合剂的流动相中。HPLC已成为痕量金属元素分析的极好方法。 相似文献
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离子色谱是分离分析阳离子型化合物的重要手段之一。高效阳离子交换固定相的制备研究对离子色谱技术的发展具有重要的意义。该文以丙烯酸和顺丁烯二酸酐为单体,2-巯基乙基磺酸钠为巯基改性剂,提出了聚合物基质微球巯基改性自由基聚合修饰方法,用以制备新型双功能的阳离子交换固定相。该固定相以羧基和磺酸基为功能基,仅用简单的强酸淋洗液便可以实现常规阳离子的基线分离。利用色谱学模型,对金属离子和有机胺的保留行为进行了研究。采用梯度淋洗模式,可在24 min内实现10种阳离子的分离,表明固定相具有优异的色谱性能。“巯基-烯”修饰方法简单、高效。此外,通过调节巯基改性剂的比例能够实现对固定相交换能力的调控。 相似文献