Dechlorane Plus and related compounds in aquatic and terrestrial biota: a review

Dechlorane Plus, dechlorane 602, dechlorane 603 and dechlorane 604 are flame retardants that have been used for a long time as a substitute for mirex, but they have not been noticed as environmental contaminants until recently (2006). Regardless of their large molecular size and very high lipophilicity (log K _OW?>?9), Dechlorane Plus and related compounds have been detected in different aquatic and terrestrial species, supporting their bioaccumulation and biomagnification. Moreover, some studies showed different behaviour of the syn-Dechlorane Plus and anti-Dechlorane Plus isomers in the environment and different biomagnification factors in biota. This review describes the different analytical approaches applied to the determination of Dechlorane Plus and related compounds. Moreover, a summary of their levels in aquatic and terrestrial biota, as well as in humans, is presented, showing also current research results on their bioaccumulation and biomagnification potential. Finally, isomer-specific bioaccumulation of Dechlorane Plus is also discussed.     

Organic UV filters in marine environments: An update of analytical methodologies,occurrence and distribution

Ultraviolet filters (UV Filters) are compounds that are widely employed in personal care products such as sunscreens to protect the skin from sun damage, but they are also added to other products, such as food packaging, plastics, paints, textiles, detergents, etc. The continuous use of these products causes the release of a substantial amount of these products into the marine environment through direct input or wastewater discharge, and thus they are becoming an important class of contaminants of emerging concern. A correlation between their occurrence and different negative effects on marine biota has been reported.Taking into account all the possible impacts on the environment, knowledge of their presence and distribution in the different compartments of the ecosystems, ranging from waters and sediments to aquatic organisms, which potentially suffer from bioaccumulation and biomagnification processes, is essential. High concentrations of ultraviolet filters have been found in samples collected from across the entire planet, even in polar regions, revealing their global distribution.Therefore, interest in the sensitive determination of ultraviolet filters in several marine matrices has increased. In this article, an overall review of the more recently reported analytical chemistry methods for identifying and quantifying these compounds in marine environmental samples is presented. We compare and discuss the potential advantages and disadvantages of every step involved in the analytical procedure, including the pre-treatment, treatment and extraction processes that are required to avoid matrix effects. Moreover, we describe the worldwide occurrence and distribution of those most important UV filters.     

Liquid chromatography methodologies for the determination of steroid hormones in aquatic environmental systems

Steroid hormones are a diverse group of natural and synthetic compounds. Their wide use in human and veterinary medicine results in their continual introduction into the environment. In recent years, environmental concern over steroids that act as endocrine disruptors has increased because of their adverse effects on organisms or their progeny. Moreover, as these compounds are not totally removed from sewage in wastewater treatment plants, they can reach the aquatic environment and persist due to their physicochemical characteristics.For this reason, a major trend in analytical chemistry is the development of rapid and efficient procedures for the extraction, determination and quantification of steroid hormones in environmental samples. Over the past few decades, the significant expansion of liquid chromatography technology utilizing mass spectrometry detection has led to applications with increased selectivity and sensitivity. Optimized extraction and microextraction techniques combined to these liquid chromatography techniques have lowered detection and quantification limits to the ng L⁻¹–μg L⁻¹ range, which is the concentration of steroid hormones in liquid, solid and biota samples.In this paper, the state-of-the-art techniques for the analysis of steroid hormones focused mainly in based liquid chromatography methods in liquid and aquatic solid and biota samples are reviewed. Handling, storage, extraction and detection methodologies are reviewed and compared for all families of steroid hormones.     

Pharmaceutical residues in environmental waters and wastewater: current state of knowledge and future research

Pollution from pharmaceuticals in the aquatic environment is now recognized as an environmental concern in many countries. This has led to the creation of an extensive area of research, including among others: their chemical identification and quantification; elucidation of transformation pathways when present in wastewater-treatment plants or in environmental matrices; assessment of their potential biological effects; and development and application of advanced treatment processes for their removal and/or mineralization. Pharmaceuticals are a unique category of pollutants, because of their special characteristics, and their behavior and fate cannot be simulated with other chemical organic contaminants. Over the last decade the scientific community has embraced research in this specific field and the outcome has been immense. This was facilitated by advances in chromatographic techniques and relevant biological assays. Despite this, a number of unanswered questions exist and still there is much room for development and work towards a more solid understanding of the actual consequences of the release of pharmaceuticals in the environment. This review tries to present part of the knowledge that is currently available with regard to the occurrence of pharmaceutical residues in aquatic matrices, the progress made during the last several years on identification of such compounds down to trace levels, and of new, previously unidentified, pharmaceuticals such as illicit drugs, metabolites, and photo-products. It also tries to discuss the main recent findings in respect of the capacity of various treatment technologies to remove these contaminants and to highlight some of the adverse effects that may be related to their ubiquitous existence. Finally, socioeconomic measures that may be able to hinder the introduction of such compounds into the environment are briefly discussed.     

Analysis of emerging and related pollutants in aquatic biota

Water bodies cover approximately 70 % of the earth´s surface, making them ecosystems with a high environmental value and the habitat for numerous species of flora and fauna. Emerging pollutants (EPs) are ubiquitous anthropogenic compounds of environmental concern that can be found at different concentration levels in matrices such as sediment, water and aquatic biota. In addition, EPs can be bioaccumulated and biomagnified, inducing adverse effects on biota, and posing a risk to humans when contaminated biota is consumed. Unlike abiotic matrices, the occurrence of EPs in aquatic biota has not been widely studied. This is probably because their complexity, due to the presence of lipids, proteins and other organic compounds, makes the extraction and analysis of EPs difficult. This review gathers the most relevant analytical methods published between 2014 and 2019, comparing them and evaluating their strengths and weaknesses. It is intended to provide a better understanding of the development of new and improved methods, and to be a reference for researchers who are looking for the best methodology for their studies.     

Occurrence patterns of pharmaceuticals in water and wastewater environments

The occurrence of pharmaceuticals and their metabolites and transformation products in the environment is becoming a matter of concern, because these compounds, which may have adverse effects on living organisms, are extensively and increasingly used in human and veterinary medicine and are released continuously into the environment. A variety of pharmaceuticals have been detected in many environmental samples worldwide. Their occurrence has been reported in sewage-treatment-plant effluents, surface water, seawater, groundwater, soil, sediment and fish. This paper provides an overview of recent scientific research on the sources, occurrence, and fate of pharmaceuticals in water and wastewater.     

Pharmaceuticals and their metabolites in the marine environment: Sources,analytical methods and occurrence

The occurrence of pharmaceuticals in the environmental waters is a global concern. There is little research conducted on the monitoring of pharmaceuticals in the marine environment. In this article, the occurrence of pharmaceuticals and their metabolites in the coastal waters as well as associated risks related to their uptake by marine organisms are critically reviewed. The literature showed antibiotics as the most plentiful pharmaceuticals in the marine environment. Other therapeutic classes of pharmaceuticals appeared prominently in the marine environment are non-steroidal anti-inflammatory drugs and β-blockers, while gemfibrozil and carbamazepine were singled-out as the most studied lipid regulator and antiepileptic, respectively. Some pharmaceuticals have been found present in the marine organisms that are regarded as important food sources for humans. We reviewed the negative effects associated with the presence of pharmaceuticals in the marine environment. This article is concluded by deliberating on the possible future studies in this research niche area.     

Rapid quantification of pharmaceuticals and pesticides in passive samplers using ultra high performance liquid chromatography coupled to high resolution mass spectrometry

The presence of both pharmaceuticals and pesticides in the aquatic environment has become a well-known environmental issue during the last decade. An increasing demand however still exists for sensitive and reliable monitoring tools for these rather polar contaminants in the marine environment. In recent years, the great potential of passive samplers or equilibrium based sampling techniques for evaluation of the fate of these contaminants has been shown in literature. Therefore, we developed a new analytical method for the quantification of a high number of pharmaceuticals and pesticides in passive sampling devices. The analytical procedure consisted of extraction using 1:1 methanol/acetonitrile followed by detection with ultra-high performance liquid chromatography coupled to high resolution and high mass accuracy Orbitrap mass spectrometry. Validation of the analytical method resulted in limits of quantification and recoveries ranging between 0.2 and 20 ng per sampler sheet and between 87.9 and 105.2%, respectively. Determination of the sampler-water partition coefficients of all compounds demonstrated that several pharmaceuticals and most pesticides exert a high affinity for the polydimethylsiloxane passive samplers. Finally, the developed analytical methods were used to measure the time-weighted average (TWA) concentrations of the targeted pollutants in passive samplers, deployed at eight stations in the Belgian coastal zone. Propranolol, carbamazepine and seven pesticides were found to be very abundant in the passive samplers. These obtained long-term and large-scale TWA concentrations will contribute in assessing the environmental and human health risk of these emerging pollutants.     

Analysis of polychlorinated n-alkanes in environmental samples

Polychlorinated n-alkanes (PCAs), also known as chlorinated paraffins (CPs), are highly complex technical mixtures that contain a huge number of structural isomers, theoretically more than 10,000 diastereomers and enantiomers. As a consequence of their persistence, tendency to bioaccumulation, and widespread and unrestricted use, PCAs have been found in aquatic and terrestrial food webs, even in rural and remote areas. Recently, these compounds have been included in regulatory programs of several international organizations, including the US Environmental Protection Agency and the European Union. Consequently, there is a growing demand for reliable methods with which to analyze PCAs in environmental samples. Here, we review current trends and recent developments in the analysis of PCAs in environmental samples such as air, water, sediment, and biota. Practical aspects of sample preparation, chromatographic separation, and detection are covered, with special emphasis placed on analysis of PCAs using gas chromatography–mass spectrometry. The advantages and limitations of these techniques as well as recent improvements in quantification procedures are discussed.     

Validation and Evaluation of Selected Organic Pollutants in Shrimp and Seawater Samples from the NW Portuguese Coast

The development of coastal regions has contributed to the intensification of environmental contamination, which can accumulate in aquatic biota, such as shrimps. These crustaceans, besides being delicious and being a good source of nutrients, can also accumulate environmental pollutants. Amongst others, these include organochlorine pesticides (OCPs), organophosphorus pesticides (OPPs), brominated flame retardants (BFRs), polychlorinated biphenyls (PCBs) and synthetic musks (SMs). These pollutants, classified as endocrine disruptors, are related to adverse effects in humans and since one of the major routes of exposition is ingestion, this is a cause for concern regarding their presence in food. The aim of the present study was to quantify the presence of environmental pollutants in shrimp samples and in the water from their habitat along the northwest Portuguese coast. In seawater samples, only two OCPs (lindane and DDD) and one BFR (BTBPE) were detected, and in shrimp samples, one OCP (DDD) and three SMs (HHCB, AHTN and ketone) were found. Bioaccumulation and the risk assessment of dietary exposure of SMs in shrimp samples were investigated. It was observed that all shrimp samples analyzed significantly presented bioaccumulation of the three SMs found. Concentrations of SMs detected in shrimp samples do not present a health risk for the adult Portuguese population.     

Determination of perfluorinated compounds in aquatic organisms: a review

Bioaccumulation of PFAS in aquatic organisms is an environmental problem of growing concern around the world. This problem has been tackled by regulatory bodies by proposing EQS for biota in EU water bodies and tolerable daily intake for food. The introduction of regulatory limits requires the availability of harmonised and validated analytical methods of sufficient sensitivity. This paper reviews recent advances in analytical methods for analysis of PFAS in aquatic organisms. The methods available for biota analysis are mostly based on three extraction procedures: ion-pair extraction, solvent liquid extraction, and alkaline digestion. The resulting extracts are then subjected to different clean-up or enrichment steps on solid adsorbents, for example graphitized carbon black, C₁₈, and WAX phases. All methods reviewed in this work give reliable results but are partially validated only, because of the lack of certified reference materials and regular interlaboratory exercises. The few interlaboratory exercises performed on real unspiked samples did not afford satisfactory results for PFAS other than PFOS, especially for matrices with high lipid content, for example mussels. The reasons for those partially negative results have been identified, and can mainly be attributed to calibration procedures and availability and purity of standards. The urgent need for certified reference materials for this type of analysis is emphasized.     

Determination of Pharmaceutical Drug Residues on Suspended Particulate Material in Surface Water

Recent studies have demonstrated the presence of traces of various pharmaceutical drugs in the aquatic environment. Comprehensive data about the distribution of such compounds between the aqueous phase and suspended particulate material are still missing. In the present article a gas chromatographic method with mass spectrometric detection is presented which allows the determination of the particle-bound fraction of some pharmaceuticals commonly found in surface water. Determination limits are between 2 and 12 ng/g particles. Results from surface water samples indicate the possibility that less hydrophilic pharmaceuticals like mefenamic acid are present as suspended particulate material, although the amounts are small in comparison with the concentrations found in aqueous phase. Additional work will be necessary to evaluate the full importance of particle-bound pharmaceuticals with respect to transportation in the environment.     

Microplastics and associated emerging contaminants in the environment: Analysis,sorption mechanisms and effects of co-exposure

Microplastics (MPs) and other emerging pollutants exist together in the environment and their co-exposure represents a source of increasing concern as MPs have been reported to act as carriers of pollutants due to their high sorption capacity. The ingestion of contaminated MPs by organisms can enhance the desorption of pollutants, increasing their bioavailability and toxicity. This review examines the role of MPs as vectors of environmental emerging contaminants. First, the main tools used to identify and characterise MPs and the analytical methods used for the determination of associated emerging contaminants are discussed. Insightful explanations of the sorption interaction between several groups of emerging pollutants and MPs are provided. MP type (polarity, crystallinity, size) and aging process together with the environmental conditions and pollutant properties (hydrophobicity and dissociated forms) are key factors influencing the sorption process. The literature review showed that polyethylene and polystyrene were the most commonly studied polymers. Antibiotics, perfluoroalkyl compounds and triclosan showed high sorption capacities onto MPs. Finally, the effect of co-exposure to MPs-emerging pollutants and bioaccumulation in aquatic and terrestrial organisms is discussed. The combined exposure may impact the toxic effects in different ways, through synergistic or antagonic interactions. Examples of different scenarios are provided, but in general the research conducted on terrestrial systems is scarce. The results revealed a lack of standardization in laboratory studies and in the testing conditions that reflect actual environmental exposure.     

Insight into the Sorption of 5-Fluorouracil and Methotrexate onto Soil–pH,Ionic Strength,and Co-Contaminant Influence

Nowadays anticancer drugs (ADs), like other pharmaceuticals, are recognized as new emerging pollutants, meaning that they are not commonly monitored in the environment; however, they have great potential to enter the environment and cause adverse effects there. The current scientific literature highlights the problem of their presence in the aquatic environment by publishing more and more results on their analytics and ecotoxicological evaluation. In order to properly assess the risk associated with the presence of ADs in the environment, it is also necessary to investigate the processes that are important in understanding the environmental fate of these compounds. However, the state of knowledge on mobility of ADs in the environment is still very limited. Therefore, the main aim of our study was to investigate the sorption potential of two anticancer drugs, 5-fluorouracil (5-FU) and methotrexate (MTX), onto different soils. Special attention was paid to the determination of the influence of pH and ionic strength as well as presence of co-contaminants (cadmium (Cd²⁺) and another pharmaceutical—metoprolol (MET)) on the sorption of 5-FU and MTX onto soil. The obtained distribution coefficient values (K_d) ranged from 2.52 to 6.36 L·kg⁻¹ and from 6.79 to 12.94 L·kg⁻¹ for 5-FU and MTX, respectively. Investigated compounds may be classified as slightly or low mobile in the soil matrix (depending on soil). 5-FU may be recognized as more mobile in comparison to MET. It was proved that presence of other soil contaminants may strongly influence their mobility in soil structures. The investigated co-contaminant (MET) caused around 25-fold increased sorption of 5-FU, whereas diminished sorption of MTX. Moreover, the influence of environmental conditions such as pH and ionic strength on their sorption has been clearly demonstrated.     

Investigation of capillary electrophoresis-laser induced fluorescence as a tool in the characterization of sewage effluent for fluorescent acids: determination of salicylic acid

The investigation of emerging contaminant issues is a proactive effort in environmental analysis. As a part of this effort, sewage effluent is of current analytical interest because of the presence of pharmaceuticals and their metabolites and personal care products. The environmental impact of these components is still under investigation but their constant perfusion into receiving waters and their potential effect on biota is of concern. This paper examines a tool for the characterization of sewage effluent using capillary electrophoresis-laser induced fluorescence (CE-LIF) with a frequency-doubled laser operated in the ultraviolet (UV). Fluorescent acidic analytes are targeted because they present special problems for techniques such as gas chromatography-mass spectrometry (GC-MS) but are readily accessible to CE-LIF. As an example of the application of this tool, salicylic acid is determined near the 100 ng/L (7 x 10(-10) M) level in sewage effluent. Salicylic acid is a metabolite of various analgesics. Relatively stable in the environment, it is a common contaminant of municipal sewage systems. Salicylic acid was recovered from freshly collected samples of the effluent by liquid-liquid extraction. Confirmation of identity was by electron ionization GC-MS after conversion of the salicylic acid to the methyl ester by means of trimethylsilyldiazomethane. CE-LIF in the UV has revealed more than 50 individual peaks in the extract and a background response that suggests a large and indeterminate number of additional compounds are present. These data together with complementary techniques provide information on the complexity and components in these effluent streams.     

Mycosporine-like Amino Acids in Freshwater Copepods: Potential Sources and Some Factors That Affect Their Bioaccumulation

Mycosporine-like amino acids (MAAs) are ubiquitous photoprotective compounds in aquatic environments. MAAs are synthesized by a wide variety of organisms (i.e. bacteria, fungi and algae) and their production is photoinducible by ultraviolet radiation (UVR) (280–400 nm) and/or photosynthetically active radiation (400–750 nm). Most animals however, are unable to synthesize MAAs and must acquire these compounds through their diet or from symbiotic organisms. In this paper, we investigate the possible sources of MAAs and factors (temperature and initial MAA concentration) that may affect their bioaccumulation in freshwater copepods. We found that MAA accumulation may occur even if the copepods are cultured on a MAA-free diet. In addition, we found that the bacteriostatic antibiotic, chloramphenicol, inhibits the bioaccumulation of MAAs. These two pieces of evidence suggest that the source of MAAs in these copepods may be prokaryotic organisms in close association with the animals. The two factors investigated in this study, temperature and initial MAA concentrations, were found to affect the rates at which MAAs are accumulated. Temperature had positive effects on both uptake and elimination rates. On the other hand, the rate of uptake decreased at the highest assayed initial MAA concentration, probably because the concentration of MAAs was already close to saturation.     

The role of solar UV radiation in the ecology of alpine lakes.

Solar ultraviolet radiation (UVR, 290-400 nm) is a crucial environmental factor in alpine lakes because of the natural increase of the UVR flux with elevation and the high water transparency of these ecosystems. The ecological importance of UVR, however, has only recently been recognized. This review, examines the general features of alpine lakes regarding UVR, summarizes what is known about the role of solar UVR in the ecology of alpine lakes, and identifies future research directions. Unlike the pattern observed in most lowland lakes, variability of UV attenuation in alpine lakes is poorly explained by differences in dissolved organic carbon (DOC) concentrations, and depends mainly on optical characteristics (absorption) of the chromophoric dissolved organic matter (CDOM). Within the water column of lakes with low DOC concentrations (0.2-0.4 mg l(-1)), UV attenuation is influenced by phytoplankton whose development at depth (i.e. the deep chlorophyll maximum) causes important changes in UV attenuation. Alpine aquatic organisms have developed a number of strategies to minimize UV damage. The widespread synthesis or bioaccumulation of different compounds that directly or indirectly absorb UV energy is one such strategy. Although most benthic and planktonic primary producers and crustacean zooplankton are well adapted to high intensities of solar radiation, heterotrophic protists, bacteria, and viruses seem to be particularly sensitive to UVR. Understanding the overall impact of UVR on alpine lakes would need to consider synergistic and antagonistic processes resulting from the pronounced climatic warming, which have the potential to modify the UV underwater climate and consequently the stress on aquatic organisms.     

Bioactive Metabolites from Marine Algae as Potent Pharmacophores against Oxidative Stress-Associated Human Diseases: A Comprehensive Review

In addition to cancer and diabetes, inflammatory and ROS-related diseases represent one of the major health problems worldwide. Currently, several synthetic drugs are used to reduce oxidative stress; nevertheless, these approaches often have side effects. Therefore, to overcome these issues, the search for alternative therapies has gained importance in recent times. Natural bioactive compounds have represented, and they still do, an important source of drugs with high therapeutic efficacy. In the “synthetic” era, terrestrial and aquatic photosynthetic organisms have been shown to be an essential source of natural compounds, some of which might play a leading role in pharmaceutical drug development. Marine organisms constitute nearly half of the worldwide biodiversity. In the marine environment, algae, seaweeds, and seagrasses are the first reported sources of marine natural products for discovering novel pharmacophores. The algal bioactive compounds are a potential source of novel antioxidant and anticancer (through modulation of the cell cycle, metastasis, and apoptosis) compounds. Secondary metabolites in marine Algae, such as phenolic acids, flavonoids, and tannins, could have great therapeutic implications against several diseases. In this context, this review focuses on the diversity of functional compounds extracted from algae and their potential beneficial effects in fighting cancer, diabetes, and inflammatory diseases.     

An overview of UV-absorbing compounds (organic UV filters) in aquatic biota

The purpose of this article is to summarize biological monitoring information on UV-absorbing compounds, commonly referred as organic UV filters or sunscreen agents, in aquatic ecosystems. To date a limited range of species (macroinvertebrates, fish, and birds), habitats (lakes, rivers, and sea), and compounds (benzophenones and camphors) have been investigated. As a consequence there is not enough data enabling reliable understanding of the global distribution and effect of UV filters on ecosystems. Both liquid chromatography and gas chromatography coupled with mass spectrometry-based methods have been developed and applied to the trace analysis of these pollutants in biota, enabling the required selectivity and sensitivity. As expected, the most lipophilic compounds occur most frequently with concentrations up to 7112?ng?g^?1 lipids in mussels and 3100?ng?g^?1 lipids (homosalate) in fish. High concentrations have also been reported for 4-methylbenzilidenecamphor (up to 1800?ng?g^?1 lipids) and octocrylene (2400?ng?g^?1 lipids). Many fewer studies have evaluated the potential bioaccumulation and biomagnification of these compounds in both fresh and marine water and terrestrial food webs. Estimated biomagnification factors suggest biomagnification in predator–prey pairs, for example bird–fish and fish–invertebrates. Ecotoxicological data and preliminary environmental assessment of the risk of UV filters are also included and discussed.