高效液相色谱法直接测定尿中尿酸和肌酐

尿中尿酸与肌酐比值的测定对于某些疾病的诊断及病理研究很有意义,但一般需先分别测出二者浓度,再计算比值,这样不仅测定周期长,而且干扰影响大,结果准确性差。采用色谱法测定尿酸和肌酐可较好地改善检测的特异性。 本文推荐了一种基于液相色谱分离,UV234nm检测的分析方法,在5分钟内可完成对尿中尿酸和肌酐的同时测定。尿样只需用流动相稀释后便可直接进样,操作简便、干扰少,是一种特异、灵敏、准确的测定方法。     

高效液相色谱法同时测定血清中肌酸、尿酸和肌酐

报道了快速、简便同时测定血清中肌酸，尿酸和肌酐的ＨＰＬＣ法．以０．０２ｍｏｌ／ＬＫＨ２ＰＯ４（ｐＨ６．５）为流动相，三种物质可在２ｍｉｎ内分离，检出限分别为：肌酸０．６９μｇ／ｍＬ，尿酸０．２１μｇ／ｍＬ，肌酐０．１０μｇ／ｍＬ．     

毛细管电泳多次进样法快速测定尿肌酐

采用毛细管电泳多次进样技术快速测定了尿肌酐。采用３６ｃｍ×５０μｍｉ．ｄ涂层柱,０．１ｍｍｏｌ／ＬｐＨ２．５的磷酸缓冲液,在２００ｎｍ处检测。连续进样５个样品总分析时间为１２ｍｉｎ,比单次进样节省２０ｍｉｎ左右。日内、日间变异系数均小于７．０％,用吡啶作内标,在肌酐浓度为５～８０ｍｇ／Ｌ范围内,肌酐浓度与肌酐同吡啶的峰高比值的线性关系很好（ｒ＝０．９９９６）。测定１２个样品同生化分析仪测定的结果相关性好（ｒ＝０．９７７３）,且分析时间节省了约３０ｍｉｎ。     

高效液相色谱法同时测定假尿核苷和肌酐的研究及应用

本文用高效液相色谱法同时测定人尿中假尿核苷和肌酐,研究了流动相的pH值变化对色谱行为的影响,方法简便、快速,已成功地用于对正常人、肺部感染及肺癌患者的尿样分析,其结果有显著性差异。     

高效液相色谱法同时测定肿瘤患者尿中假尿苷和肌酐的含量

An HPLC method for the simultaneous determination of pseudouridine(Pu)and Cretinine(C)in urinewas established;The recoveries of Pu and C were 92. 09±3.5%and 83. 01±5.7%respectively. The calibra-tion curves were linear within the concentration range of 2～200μg/mL for Pu and 5～300μg/mL for C. The detection linilts for Pu and C were 2. 15μg/mL and 4.75μg/mL. The method is rapid,sensitive and reliable.     

反相高效液相色谱法同时测定血清、尿液中尿酸、肌酐的含量

建立了同时测定人体血清、尿液中肌酐、尿酸含量的高效液相色谱分析方法。采用Hypersil ODS C18色谱柱,甲醇(A)-20 mmol/L乙酸铵(B)为流动相,梯度洗脱,流速1.0 mL/min,UV检测,检测波长235 nm。血清中肌酐、尿酸含量在2~20μg/mL范围内线性关系良好,尿液中肌酐、尿酸在5~1000μg/mL范围内线性关系良好;平均加标回收率在86.7%~99.0%,RSD小于5%。选择甲醇-乙酸铵作为流动相进行血清/尿液中肌酐、尿酸含量测定具有快速简便的特点,方法应用于健康人和糖尿病病人血清/尿液样本的测定,可以对临床病人进行快速监测。     

高效毛细管区带电泳测定人尿液中尿酸、肌酐和伪尿核苷的含量

报道了采用高效毛细管区带电泳技术直接将人尿液注入毛细管进行尿液中肌酐、尿酸及伪尿核苷含量测定的新方法。试验表明,以磷酸盐（ｐＨ６．１）作缓冲液,对人体尿液中肌酐、尿酸及伪尿核苷进行直接分析具有较高的灵敏度和较好的重复性。     

HPLC同时检测血清和尿样中肌酐、假尿苷、尿酸

用反相高效液相色谱法同时测定了３９例糖尿病患者的血清和尿样中的假尿嘧啶核苷、肌酐和尿酸,并与２４ｈ尿白蛋白排泄量进行了比较分析。发现血清假尿嘧啶核苷是糖尿病肾病早期诊断的一种新颖而敏感的指标,有助于连续监测肾脏的功能状态以了解病程的转归。     

反相高效液相色谱法直接测定尿中微量酚

The separation of the phenol from complex components after the acidolysis of the urinary sample wascarried out on a PE 3 × 3-C(R-C_(18) column(4. 6mm× 30mm) by using buffer solution of phosphate and methanolas mobile phase. The method is simple,rapid and accurate for practical use and applicable to the quantitative determination of phenol.Satisfactory results were achieved.     

微透析——高效液相色谱法测定病人血清中肌酐含量

以微透析作为样品预处理技术，与高效液相色谱分析相结合建立了一个测定血清样品中肌酐浓度的方法。同时考察了温度、灌注流速对对微透析取样回收率的影响。微透析取样在３７℃、灌注流速为２．０μＬ／ｍｉｎ时回收率２２．２９％，ＲＳＤ８．２７％，利用这种方法病人血清中肌酐浓度，简单、快速、重复性较好。     

利用界面反应集衍生富集为一体的HPLC测定尿样中三氯乙酸的研究

基于吡啶与三氯乙酸的界面反应,建立了一种高效液相色谱测定尿液中三氯乙酸的分析方法。通过正交实验及方差分析确定了界面反应中各条件无明显的相关性,考察了尿样体积、衍生试剂体积、碱浓度、反应时间及温度等参数对反应的影响,并优化了色谱分离条件。在最佳实验条件下,建立了待测组分的工作曲线,测得三氯乙酸的线性范围为0.002 0~20.0 mg/L(r=0.999 7),方法检出限(S/N=3)为0.1μg/L,样品的加标回收率为91.9%~105%,相对标准偏差(RSD)为3.5%~4.6%。研究结果表明,利用这种界面反应可同时达到富集痕量待测组分及纯化样品的作用,有效地降低了方法的检出限,方法具有简便、快速、准确和对环境友好的特点。     

Urinary Uric Acid Determination by Reversed-Phase High Pressure Liquid Chromatography

Abstract

Reversed-phase high pressure liquid chromatography with UV detection was proven to be a powerful method for the separation and quantitation of urinary uric acid. We have compared three different treatments for urine samples previous chromatographic injection: alkaline methanol extraction, ethylacetate extraction and centrifugation. It was also studied storage conditions for urine samples.

Our findings show that the method has high specificity and reproducibility for urinary uric acid. Samples are stables and require only centrifugation previous injection to the chromatograph.     

The Determination of Ioglycamic Acid in Bile and in Urine by High Performance Liquid Chromatography

Abstract

A fast, simple and specific method for the determination of ioglycamic acid in bile and in urine is described. Reversed-phase ion-pair chromatography with methanol-water (67.5: 32.5) in the presence of the counter ion tetrabutyl-ammonium phosphate as the mobile phase separates ioglycamic acid in 5 min at a flow rate of 1 ml/min. Under the same conditions urinary creatinine can be simultaneously determined and may be used as an endogenous internal standard. Bile and urine from a healthy subject were screened for possible metabolites but these are not detected.     

Determination of Sparfloxacin in Human Urine by Reversed-Phase High Performance Liquid Chromatography With Nitrous Acid and Hydroiodic Pre-Column Derivatization

Quinolone drugs, due to the aromatic systems with benzoheterocycle in their molecules, can usually emit strong fluorescence. However, contrary to this, sparfloxacin (SPFX) as the fourth generation quinolone products, can only emit weak fluorescence, although it possesses a similar structure to quinolones. At present, the majority of SPFX determinations have been carried out using techniques such as colorimetry1 and spectrophotometry2-3. For the high performance liquid chromatography (HP…     

Determination of Propylthiouracil in Human Breast Milk by Direct Injection High Performance Liquid Chromatography

Abstract

A high-performance liquid chromatographic(HPLC) method was developed for the assay of propylthiouracil in human breast milk. After filtration with membran filter(Molcut II), the eluent was injected into a liquid chromatogaph equipped with C₁₈ precolumn and analytical column in series according to column switching techniques. This method is sufficiently sensitive for most pharmacokinetic studies in human breast milk. The concentration of propylthiouracil was linear over the 50 – 5000ng/ml range. The recovery and the coefficient of variation was 92.0 – 100.6% and 1.6 – 2.9%, respectivery. This assay has the advantages of specificity, simplicity and reproducibility for the measurement of propylthiouracil in human breast milk.