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1.
Ruijie Fu Kai Yao Qisheng Zhang Dongying Jia Jiayuan Zhao Yuanlong Chi 《Applied biochemistry and biotechnology》2017,182(1):55-66
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes. 相似文献
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Adam Elliston Samuel R. A. Collins Craig B. Faulds Ian N. Roberts Keith W. Waldron 《Applied biochemistry and biotechnology》2014,172(7):3621-3634
Waste copier paper is a potential substrate for the production of glucose relevant for manufacture of platform chemicals and intermediates, being composed of 51 % glucan. The yield and concentration of glucose arising from the enzymatic saccharification of solid ink-free copier paper as cellulosic substrate was studied using a range of commercial cellulase preparations. The results show that in all cellulase preparations examined, maximum hydrolysis was only achieved with the addition of beta-glucosidase, despite its presence in the enzyme mixtures. With the use of Accellerase® (cellulase), high substrate loading decreased conversion yield. However, this was overcome if the enzyme was added between 12.5 and 20 FPU g substrate?1. Furthermore, this reaction condition facilitated continual stirring and enabled sequential additions (up to 50 % w/v) of paper to be made to the hydrolysis reaction, degrading nearly all (99 %) of the cellulose fibres and increasing the final concentration of glucose whilst simultaneously making high substrate concentrations achievable. Under optimal conditions (50 °C, pH 5.0, 72 h), digestions facilitate the production of glucose to much improved concentrations of up to 1.33 mol l?1. 相似文献
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Sweet Sorghum as Feedstock for Ethanol Production: Enzymatic Hydrolysis of Steam-Pretreated Bagasse 总被引:1,自引:0,他引:1
Bálint Sipos Jutka Réczey Zsolt Somorai Zsófia Kádár Dóra Dienes Kati Réczey 《Applied biochemistry and biotechnology》2009,153(1-3):151-162
Sweet sorghum is an attractive feedstock for ethanol production. The juice extracted from the fresh stem is composed of sucrose, glucose, and fructose and can therefore be readily fermented to alcohol. The solid fraction left behind, the so-called bagasse, is a lignocellulosic residue which can also be processed to ethanol. The objective of our work was to test sweet sorghum, the whole crop, as a potential raw material of ethanol production, i.e., both the extracted sugar juice and the residual bagasse were tested. The juice was investigated at different harvesting dates for sugar content. Fermentability of juices extracted from the stem with and without leaves was compared. Sweet sorghum bagasse was steam-pretreated using various pretreatment conditions (temperatures and residence times). Efficiency of pretreatments was characterized by the degree of cellulose hydrolysis of the whole pretreated slurry and the separated fiber fraction. Two settings of the studied conditions (190 °C, 10 min and 200 °C, 5 min) were found to be efficient to reach conversion of 85–90%. 相似文献
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Henghui Zhang Zhijun Zhang Dongliang He Shuying Li Yongping Xu 《Molecules (Basel, Switzerland)》2022,27(3)
Botanical oils are staple consumer goods globally, but as a by-product of oil crops, meal is of low utilization value and prone to causing environmental problems. The development of proteins in meal into bioactive peptides, such as Perilla peptide, through biotechnology can not only solve environmental problems, but also create more valuable nutritional additives. In the present work, the hydrolysis process of Perilla meal protein suitable for industrial application was optimized with the response surface methodology (RSM) on the basis of single-factor experiments. Alcalase was firstly selected as the best-performing among four proteases. Then, based on Alcalase, the optimal hydrolysis conditions were as follows: enzyme concentration of 7%, hydrolysis temperature of 61.4 °C, liquid-solid ratio of 22.33:1 (mL/g) and hydrolysis time of 4 h. Under these conditions, the degree of hydrolysis (DH) of Perilla meal protein was 26.23 ± 0.83% and the DPPH scavenging capacity of hydrolysate was 94.15 ± 1.12%. The soluble peptide or protein concentration of Perilla meal protein hydrolysate rose up to 5.24 ± 0.05 mg/mL, the ideal yield of which was estimated to be 17.9%. SDS-PAGE indicated that a large proportion of new bands in hydrolysate with small molecular weights appeared, which was different from the original Perilla meal protein. The present data contributed to further, more specific research on the separation, purification and identification of antioxidant peptide from the hydrolysate of Perilla meal protein. The results showed that the hydrolysis of Perilla meal protein could yield peptides with high antioxidant activity and potential applications as natural antioxidants in the food industry. 相似文献
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Pretreatment of Rice Straw by a Hot-Compressed Water Process for Enzymatic Hydrolysis 总被引:1,自引:0,他引:1
Guoce Yu Shinichi Yano Hiroyuki Inoue Seiichi Inoue Takashi Endo Shigeki Sawayama 《Applied biochemistry and biotechnology》2010,160(2):539-551
Hot-compressed water (HCW) is among several cost-effective pretreatment processes of lignocellulosic biomass for enzymatic
hydrolysis. The present work investigated the characteristics of HCW pretreatment of rice straw including sugar production
and inhibitor formation in the liquid fraction and enzymatic hydrolysis of pretreated material. Pretreatment was carried out
at a temperature ranging from 140 to 240 °C for 10 or 30 min. Soluble oligosaccharides were found to constitute almost all
the components of total sugars in the liquid fraction. The maximal production of total glucose at 180 °C and below accounted
for 4.4–4.9% of glucan in raw material. Total xylose production peaked at 180 °C, accounting for 43.3% of xylan in raw material
for 10-min pretreatment and 29.8% for 30-min pretreatment. The production of acetic acid increased at higher temperatures
and longer treatment time, indicating more significant disruption of lignocellulosic structure, and furfural production achieved
the maximum (2.8 mg/ml) at 200 °C for both 10-min and 30-min processes. The glucose yield by enzymatic hydrolysis of pretreated
rice straw was no less than 85% at 180 °C and above for 30-min pretreatment and at 200 °C and above for 10-min pretreatment.
Considering sugar recovery, inhibitor formation, and process severity, it is recommended that a temperature of 180 °C for
a time of 30 min can be the most efficient process for HCW pretreatment of rice straw. 相似文献
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Daniel Ludwig Buchmann Michael Thomas Hirth Steffen Rupp Susanne Zibek 《Applied biochemistry and biotechnology》2014,172(3):1699-1713
In this study, we present a powerful stirred tank reactor system that can efficiently hydrolyse lignocellulosic material at high solid content to produce hydrolysates with glucose concentration > 100 g/kg. As lignocellulosic substrates alkaline-pretreated wheat straw and organosolv-pretreated beech wood were used. The developed vertical reactor was equipped with a segmented helical stirrer, which was specially designed for high biomass hydrolysis. The stirrer was characterised according to mixing behaviour and power input. To minimise the cellulase dosage, a response surface plan was used. With the empirical relationship between glucose yield, cellulase loading and solid content, the minimal cellulase dosage was calculated to reach at least 70 % yield at high glucose and high substrate concentrations within 48 h. The optimisation resulted in a minimal enzyme dosage of 30 FPU/g dry matter (DM) for the hydrolysis of wheat straw and 20 FPU/g DM for the hydrolysis of beech wood. By transferring the hydrolysis reaction from shaking flasks to the stirred tank reactor, the glucose yields could be increased. Using the developed stirred tank reactor system, alkaline-pretreated wheat straw could be converted to 110 g/kg glucose (76 %) at a solid content of 20 % (w/w) after 48 h. Organosolv-pretreated beech wood could be efficiently hydrolysed even at 30 % (w/w) DM, giving 150 g/kg glucose (72 %). 相似文献
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S. N. Olsen K. Borch N. Cruys-Bagger P. Westh 《Applied biochemistry and biotechnology》2014,174(1):146-155
Industrially, enzymatic hydrolysis of lignocellulose at high solid content is preferable over low solids due to a reduction in processing costs. Unfortunately, the economic benefits are counteracted by a linear decrease in yield with solid content, referred to as the “solid effect” in the literature. In the current study, we investigate the contribution of product inhibition to the solid effect (7–33 % solids). Product inhibition was measured directly by adding glucose to high-solid hydrolysis samples and indirectly through variation of water content and beta-glucosidase concentration. The results suggest that the solid effect is mainly controlled by product inhibition under the given experimental conditions (washed pretreated corn stover as substrate). Cellobiose was found to be approximately 15 times more inhibitory than glucose on a molar scale. However, considering that glucose concentrations are at least 100 times higher than cellobiose concentrations under industrial conditions, glucose inhibition of cellulases is suggested to be the main cause of the solid effect. 相似文献
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The use of recovered paper and paper manufacturing wastes are a potentially large, concentrated, and convenient raw material
for ethanol production via enzyme hydrolysis and fermentation. However, many previous studies in the area have investigated
impractically high enzyme charges. In this research, low dosages of enzymes on copy paper (CP) were investigated for the conditions
of 5% consistency (w/v) and 50 °C for 48 h. The removal of inorganic filler (mainly calcium carbonate) by washing prior to hydrolysis led to higher
sugar yields than the unwashed CP as well as CP acidified to remove the ash. Enzyme adsorption measurement showed that both
acid-soluble ash and acid-insoluble ash adsorb enzymes with a greater affinity than fibers. Drying of the fibers (termed hornification)
decreased the efficiency of enzyme hydrolysis, confirming previous results. The mechanical refining at 10% consistency in
a laboratory refining mill of previously dried fibers improved the sugar recovery to similar or higher levels as never-dried
fibers. By plotting water retention value (WRV) versus corresponding sugar recovery, it was shown that WRV is more useful
at low enzyme charges reflecting the use of refining and the reversal of hornification. For de-ashed and refined copy paper,
the sugar recovery was determined to be 82% and 97% with enzyme dosages of 4 and 8 Filter Paper Unit (FPU)/g oven-dried (OD)
substrate. 相似文献
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Xue Y Rusli J Chang HM Phillips R Jameel H 《Applied biochemistry and biotechnology》2012,166(4):839-855
Process simulation and lab trials were carried out to demonstrate and confirm the efficiency of the concept that recycling
hydrolysate at low total solid enzymatic hydrolysis is one of the options to increase the sugar concentration without mixing
problems. Higher sugar concentration can reduce the capital cost for fermentation and distillation because of smaller retention
volume. Meanwhile, operation cost will also decrease for less operating volume and less energy required for distillation.
With the computer simulation, time and efforts can be saved to achieve the steady state of recycling process, which is the
scenario for industrial production. This paper, to the best of our knowledge, is the first paper discussing steady-state saccharification
with recycling of the filtrate form enzymatic hydrolysis to increase sugar concentration. Recycled enzymes in the filtrate
(15–30% of the original enzyme loading) resulted in 5–10% higher carbohydrate conversion compared to the case in which recycled
enzymes were denatured. The recycled hydrolysate yielded 10% higher carbohydrate conversion compared to pure sugar simulated
hydrolysate at the same enzyme loading, which indicated hydrolysis by-products could boost enzymatic hydrolysis. The high
sugar concentration (pure sugar simulated) showed inhibition effect, since about 15% decrease in carbohydrate conversion was
observed compared with the case with no sugar added. The overall effect of hydrolysate recycling at WinGEMS simulated steady-state
conditions with 5% total solids was increasing the sugar concentration from 35 to 141 g/l, while the carbohydrate conversion
was 2% higher for recycling at steady state (87%) compared with no recycling strategy (85%). Ten percent and 15% total solid
processes were also evaluated in this study. 相似文献
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Yu Wang Shun Wang Guangdong Zhu Jianjun Xie Zhan Chen Ying Shi 《Molecules (Basel, Switzerland)》2022,27(8)
Developing new coating modification technology of aluminum nitride (AlN) powder for higher hydrolysis resistance is the key to prepare high-performance AlN ceramic substrate with water-based wet process in the future. In the this paper, The poly(vinyl pyrrolidone)-b-poly(Styrene/Itaconic anhydride) (PVP-b-P(St/ITA))block copolymer with PVP as the independent chain segment was designed and synthesized through reversible addition fragmentation chain transfer (RAFT) polymerization, which was used for the study on coating modification, hydrolysis resistance, and dispersion performance of AIN powder. The study results show that, when using PVP macromolecular chain transfer agent (PVP-CTA) for the RAFT chain extension and polymerization in St/ITA binary system, the molecular weight increases linearly and the molecular weight distribution tends to decrease with the monomer conversion rate, which is in line with the activity-controlled characteristics of RAFT polymerization. The copolymer PVP-b-P(St/ITA) was used to for surface modification treatment of submicron AlN powder to generate esterification reaction, which was absorbed and bound to the powder surface. Hydrolysis resistance and dispersion experiments were conducted for modified powder, and the crystal phase and micro structure of modified powder were analyzed and observed through XRD, SEM, and TEM. It was found that copolymer modification had no effect on the powder crystal phase. A 8–21 nm passivation layer was coated on the surface, which can exist stably for 10 h in 60 °C water. Zeta potential and laser particle analyzer tests showed that modified powder featured excellent water-based slurry dispersion performance, and certain self-dispersing characteristics. The highest Zeta potential appeared in pH 6~7, and the particle granularity was distributed uniformly with the median particle diameter of 875 nm. The powder hydrolysis resistance and dispersion performance are significantly improved. 相似文献
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功能性CdS纳米荧光探针荧光增敏法测定人血清白蛋白 总被引:11,自引:1,他引:11
目前 ,以荧光分析法对蛋白质进行研究主要采用有机荧光探针[1~ 3 ] .与传统的有机染料 (如罗丹明 )探针相比 ,半导体纳米晶体探针的光强度要高 2 0倍 ,光稳定性要高 1 0 0倍 ,谱线宽度只是有机染料谱线宽度的 1 /3 [4 ] .将半导体纳米晶体作为探针用于测定生物分子 ,将大大提高分析的灵敏度和选择性 ,而目前其应用于生物染色、医疗诊断、DNA序列测定和免疫分析等方面的研究很少 [4 ,5] .本文合成了胶态纳米粒子 Cd S,并在其外表面修饰一层巯基乙酸 ,使其具有水溶性 ,并能与生物分子作用 ,从而可利用其外表面的功能性基团对人血清白蛋白进… 相似文献
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Stefan Wunderlich Manfred Zähres Byung-Wook Jo Michael Hess 《Macromolecular Symposia》2007,258(1):101-107
Summary: We have used warfarin as a local probe to investigate the orientation of paclitaxel and water soluble polymer conjugates of paxlitaxel in albumin. The relative orientation of warfarin and paclitaxel in a 1:1 complex in solution was investigated by 1H-NMR-spektroskopy (NOESY) and the results are used for the interpretation of the steric situation of paclitaxel respectively the polymer conjugate in albumin. 相似文献
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Russian Journal of Applied Chemistry - Industrial by product dumped from iron making industry (slag) has been modified by various chemical agents, particularly, paraffin wax, liquid nitrile... 相似文献
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S. O. Bachurin D. V. Vinogradova E. F. Shevtsova T. V. Goreva T. A. Epishina A. Yu. Aksinenko V. B. Sokolov 《Russian Chemical Bulletin》2013,62(3):816-819
An approach to the modification of biologically active gamma-carbolines with the N-substituted propionamide fragments by the reaction of N-substituted acrylamides with gamma-carbolines catalyzed by cesium fluoride has been suggested. The compounds synthesized were studied for the influence on the functional characteristics of brain mitochondria, in particular, on the membrane potential, calcium-induced “swelling” and calcium capasity of the rat brain mitochondria. 相似文献