共查询到20条相似文献,搜索用时 15 毫秒
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Laurent A. Baumes Dr. Manuel Moliner Dr. Avelino Corma Dr. 《Chemistry (Weinheim an der Bergstrasse, Germany)》2009,15(17):4258-4269
Finding a clear route to new structures : The design of an adaptable time warping (ATW) methodology (see figure) for automatically, quickly, and reliably deciphering X‐ray diffraction patterns is described.
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A FRET Probe for Cell‐Based Imaging of Ganglioside‐Processing Enzyme Activity and High‐Throughput Screening 下载免费PDF全文
Dr. Guang‐Yu Yang Dr. Caishun Li Dr. Michael Fischer Prof. Christopher W. Cairo Prof. Yan Feng Prof. Stephen G. Withers 《Angewandte Chemie (International ed. in English)》2015,54(18):5389-5393
Gangliosides are important signaling molecules in the cell membrane and are processed by several enzymes. Deficiencies in these enzymes can cause human lysosomal storage diseases. Building an understanding of the pathways of glycosphingolipid catabolism requires methods for the analysis of these enzymatic activities A GM3‐derived FRET probe was synthesized chemoenzymatically for the detection and quantitation of a range of ganglioside‐degrading enzymes, both in cell lysates and in living cells. This is the first substrate that enables the ratiometric fluorogenic assay of sphingolipid ceramide N‐deacylase and endoglycoceramidase and can detect and localize neuraminidase activity in living cells. It is therefore a valuable tool for building a better understanding of membrane‐confined enzymology. It also enables the robust and reliable assay of ganglioside‐degrading enzymes in a microtiter plate, thus opening the door to screening for novel or engineered biocatalysts or for new inhibitors. 相似文献
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Daryoosh Beigzadeh 《大分子反应工程》2007,1(3):331-337
In high‐throughput research, it is essential to use “right data” and “meaningful parameters” to reach reliable conclusions. The complexity and the large amount of data obtained from each set of experiments make the analysis of reaction data a nontrivial task. The important role of reaction kinetic modeling in the analysis of polymerization reaction data is discussed, and it is shown that the application of traditional methods for the determination of catalyst productivity can be misleading. Reaction kinetic modeling provides meaningful parameters for data analysis, gives complete information about the polymerization kinetic profile, and makes it possible to evaluate assumptions and hypotheses.
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Mesenchymal‐Mode Migration Assay and Antimetastatic Drug Screening with High‐Throughput Microfluidic Channel Networks 下载免费PDF全文
Dr. Yuanqing Zhang Dr. Weijia Zhang Prof. Lidong Qin 《Angewandte Chemie (International ed. in English)》2014,53(9):2344-2348
Increasing evidence shows that activated mesenchymal migration is a key process of the metastatic cascade. Cancer cells usually gain such migratory capability through an epithelial‐to‐mesenchymal transition. Herein we present a high‐throughput microfluidic device with 3120 microchambers to specifically monitor mesenchymal migration. Through imaging of the whole chip and statistical analysis, we can evaluate the two key factors of velocity and percentage related to cell migratory capacity at different cell densities in culture. We also used the device to screen antimetastatic drugs for their inhibition of mesenchymal migration and prevention of metastatic malignancy. This device will provide an excellent platform for biologists to gain a better understanding of cancer metastasis. 相似文献
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High‐Throughput Development of a Hybrid‐Type Fluorescent Glutamate Sensor for Analysis of Synaptic Transmission 下载免费PDF全文
Dr. Kenji Takikawa Dr. Daisuke Asanuma Dr. Shigeyuki Namiki Hirokazu Sakamoto Tetsuro Ariyoshi Naoya Kimpara Prof. Kenzo Hirose 《Angewandte Chemie (International ed. in English)》2014,53(49):13439-13443
Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high‐throughput screening system, designated hybrid‐type fluorescence indicator development (HyFInD), to identify optimal position‐specific fluorophore labeling in hybrid‐type sensors consisting of combinations of ligand‐binding protein mutants with small molecular fluorophores. We screened sensors for glutamate among hybrid molecules obtained by the reaction of four cysteine‐reactive fluorescence probes with a set of cysteine‐scanning mutants of the 274 amino acid S1S2 domain of AMPA‐type glutamate receptor GluA2 subunit. HyFInD identified a glutamate‐responsive probe (enhanced glutamate optical sensor: eEOS) with a dynamic range >2400 %, good photostability, and high selectivity. When eEOS was specifically tethered to neuronal surfaces, it reliably visualized the spatiotemporal dynamics of glutamate release at single synapses, revealing synapse‐to‐synapse heterogeneity of short‐term plasticity. 相似文献
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Combinatorial libraries of segmented polyurethaneurea with gradients in curing temperature were prepared and characterized using a novel high‐throughput mechanical instrument. Stress/strain profiles were taken at different temperature positions on the libraries, and a structure/property relationship between microstructure and mechanical properties was established by correlating the measured strength and strain at break to high‐throughput AFM and FT‐IR measurements on the same library. These results demonstrate the feasibility of rapid and accurate screening of mechanical properties, and their correlation to structure, by using gradient combinatorial polymer libraries.
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Richard Hoogenboom Michael A. R. Meier Ulrich S. Schubert 《Macromolecular rapid communications》2003,24(1):15-32
Combinatorial techniques, parallel experimentation and high‐throughput methods represent a very promising approach in order to speed up the preparation and investigation of new polymeric materials: a large variety of parameters can be screened simultaneously resulting in new structure/property relationships. The field of polymer research seems to be perfectly suited for parallel and combinatorial methods due to the fact that many parameters can be varied during synthesis, processing, blending as well as compounding. In addition, numerous important parameters have to be investigated, such as molecular weight, polydispersity, viscosity, hardness, stiffness and other application‐specific properties. A number of corresponding high‐throughput techniques have been developed in the last few years and their introduction into the commercial market further boosted the development. These combinatorial approaches can reduce the time‐to‐market for new polymeric materials drastically compared to traditional approaches and allow a much more detailed understanding of polymers from the macroscopic to the nanoscopic scale. Here we provide an overview of the present status of combinatorial and parallel polymer synthesis and high‐throughput screening.
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Vincenzo Busico Roberta Pellecchia Francesco Cutillo Roberta Cipullo 《Macromolecular rapid communications》2009,30(20):1697-1708
High–throughput‐screening (HTS) tools and methods are used more and more, especially in industry, in the search for new, selective organometallic catalysts. In most cases, the approach is, in essence, empirical, and the strategy is to increase the number of experiments that can be run at a given place in a given time. Highly miniaturized, parallel reaction setups have been implemented for the rapid assessment of whether novel catalysts resulting from the structural amplification of a basic framework are “good” or “bad” with respect to the properties of interest, and, depending on the response, worthy of a subsequent, more‐careful evaluation. In this article, we demonstrate that it is possible to utilize these state‐of‐the‐art HTS platforms with a different strategy: the rapid generation of reliable kinetic data for mechanistic studies in view of a thorough understanding and rational catalyst design. Ziegler–Natta‐type catalytic olefin polymerization will be used throughout as an example.
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Michael R. Zonca Jr. Philip S. Yune Caryn L. Heldt Georges Belfort Yubing Xie 《Macromolecular bioscience》2013,13(2):177-190
Appropriate surface attachment is essential for growing embryonic stem (ES) cells in an undifferentiated state. It is challenging to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a rapid, high‐throughput polymerization and screening platform with a comprehensive library of 66 monomer‐grafted membrane surfaces, the optimal substrate, N‐[3‐(dimethylamino)propyl] methacrylamide (DMAPMA) has been identified to support strong attachment, high expansion capacity, and long‐term self‐renewal of ES cells (up to 7 passages). This monomer‐based, chemically defined, scalable, sustainable, relatively inexpensive, covalently grafted, and controllable polymeric substrate provides a new opportunity to manipulate surface chemistry for pluripotent stem culture.
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James Merrington Philip Hodge Stephen Yeates 《Macromolecular rapid communications》2006,27(11):835-840
Summary: An approach for the high‐throughput preparation and characterisation of aqueous pigment dispersions is described and evaluated. The use of ultrasonication as a rapid technique for dispersing pigments using polymeric dispersants was developed. The results are comparable to those obtained using time‐intensive conventional high‐energy ball mill processing. The quality of the pigment dispersion was evaluated in a high‐throughput fashion using digital image analysis and the results correlated with particle sizes, determined by photo‐correlation spectroscopy.
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Cornelius T. Martha Anton Heemskerk Jan‐Carel Hoogendoorn Niels Elders Wilfried M. A. Niessen Prof. Dr. Romano V. A. Orru Prof. Dr. Hubertus Irth Prof. Dr. 《Chemistry (Weinheim an der Bergstrasse, Germany)》2009,15(30):7368-7375
Optimising synthetic conversions and assessing catalyst performance is a tedious and laborious endeavour. Herein, we present an automated alternative to the commonly applied sequential approaches that are used to increase catalyst discovery process efficiencies by increasing the number of entities that can be tested. This new approach combines conversion of the reactants and determination of product formation into a single comprehensive reaction detection system that can be operated with minimal catalyst and reactant consumption. With this approach, rudimentary reaction conditions can be quickly optimised and the same system can then be used to screen for the optimal homogenous catalyst in a selected solution‐phase synthetic conversion. The system, which is composed of standard HPLC components, can be used to screen catalyst libraries at a repetition rate of five minutes and can be run unsupervised. The sensitive mass spectrometric detection that is implemented in the reaction detection methodology can be used for the simultaneous monitoring of reactants, catalysts and product ions. In the experiments, the three‐component reaction that gives a substituted 2‐imidazoline was optimised. Afterwards, the same method was used to assess a library of ferrocene‐based Lewis acid catalysts for performance in the aforementioned conversion in six different solvents. We demonstrate the feasibility of using this methodology to directly compare the performance results obtained in different solvents by calibrating the solvent‐specific MS responses. 相似文献
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Carl G. Simon Naomi Eidelman Yan Deng Newell R. Washburn 《Macromolecular rapid communications》2004,25(24):2003-2007
Summary: A method for rapidly determining the modulus of polymer blends was developed. A polymer blend gradient library of poly(L ‐lactic acid) (PLLA) and poly(D ,L ‐lactic acid) (PDLLA) was created in the form of a strip‐shaped film and characterized with FTIR microspectroscopy. Nanoindentation measurements were made along the gradients to obtain modulus data over a wide range of PLLA‐PDLLA blend compositions. This novel, high‐throughput approach to material characterization provides engineers with a technique to accelerate the development of materials.
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Jinpeng Wang Qiang Gong Nupur Maheshwari Michael Eisenstein Mary Luz Arcila Kenneth S. Kosik Prof. H. Tom Soh 《Angewandte Chemie (International ed. in English)》2014,53(19):4796-4801
We report an aptamer discovery technology that reproducibly yields higher affinity aptamers in fewer rounds compared to conventional selection. Our method (termed particle display) transforms libraries of solution‐phase aptamers into “aptamer particles”, each displaying many copies of a single sequence on its surface. We then use fluorescence‐activated cell sorting (FACS) to individually measure the relative affinities of >108 aptamer particles and sort them in a high‐throughput manner. Through mathematical analysis, we identified experimental parameters that enable optimal screening, and demonstrate enrichment performance that exceeds the theoretical maximum achievable with conventional selection by many orders of magnitude. We used particle display to obtain high‐affinity DNA aptamers for four different protein targets in three rounds, including proteins for which previous DNA aptamer selection efforts have been unsuccessful. We believe particle display offers an extraordinarily efficient mechanism for generating high‐quality aptamers in a rapid and economic manner, towards accelerated exploration of the human proteome. 相似文献
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A High‐Throughput Assay for Arylamine Halogenation Based on a Peroxidase‐Mediated Quinone–Amine Coupling with Applications in the Screening of Enzymatic Halogenations 下载免费PDF全文
Joseph Hosford Dr. Sarah A. Shepherd Prof.Dr. Jason Micklefield Dr. Lu Shin Wong 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(50):16759-16763
Arylhalides are important building blocks in many fine chemicals, pharmaceuticals and agrochemicals, and there has been increasing interest in the development of more “green” halogenation methods based on enzyme catalysis. However, the screening and development of new enzymes for biohalogenation has been hampered by a lack of high‐throughput screening methods. Described herein is the development of a colorimetric assay for detecting both chemical and enzymatic arylamine halogenation reactions in an aqueous environment. The assay is based on the unique UV/Vis spectrum created by the formation of an ortho‐benzoquinone‐amine adduct, which is produced by the peroxidase‐catalysed benzoquinone generation, followed by Michael addition of either a halogenated or non‐halogenated arylamine. This assay is sensitive, rapid and amenable to high‐throughput screening platforms. We have also shown this assay to be easily coupled to a flavin‐dependent halogenase, which currently lacks any convenient colorimetric assay, in a “one‐pot” workflow. 相似文献
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Chiral Amine Synthesis Using ω‐Transaminases: An Amine Donor that Displaces Equilibria and Enables High‐Throughput Screening 下载免费PDF全文
Dr. Anthony P. Green Prof. Nicholas J. Turner Dr. Elaine O'Reilly 《Angewandte Chemie (International ed. in English)》2014,53(40):10714-10717
The widespread application of ω‐transaminases as biocatalysts for chiral amine synthesis has been hampered by fundamental challenges, including unfavorable equilibrium positions and product inhibition. Herein, an efficient process that allows reactions to proceed in high conversion in the absence of by‐product removal using only one equivalent of a diamine donor (ortho‐xylylenediamine) is reported. This operationally simple method is compatible with the most widely used (R)‐ and (S)‐selective ω‐TAs and is particularly suitable for the conversion of substrates with unfavorable equilibrium positions (e.g., 1‐indanone). Significantly, spontaneous polymerization of the isoindole by‐product generates colored derivatives, providing a high‐throughput screening platform to identify desired ω‐TA activity. 相似文献
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Stefan Schmatloch Michael A. R. Meier Ulrich S. Schubert 《Macromolecular rapid communications》2003,24(1):33-46
Starting in biochemistry and pharmaceutical chemistry, combinatorial methods, automated synthesis and high‐throughput characterization are being further developed for organic synthesis and polymer research. The development is strongly driven by the achievements in biochemistry and pharmaceutical chemistry and the need to minimize the time‐to‐market for novel polymeric products. The success of high‐throughput methodologies in polymer science is partially limited by the commercially available hardware (synthesizers, workstations, robots, online‐characterization instruments, etc.) and software. A short overview of commercially available equipment for polymer research is provided in this Review.