共查询到19条相似文献,搜索用时 125 毫秒
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用低交换容量聚苯乙烯型强酸性阳离子交换树脂柱色谱分离中性氨基酸 总被引:4,自引:0,他引:4
通过磺化苯乙烯 -二乙烯苯共聚物或商品化的聚苯乙烯型强酸性阳离子交换树脂 ( 0 0 1× 7)的逆磺化反应 ,得到一系列不同交换容量的聚苯乙烯型强酸性阳离子交换树脂 .研究了丙氨酸和缬氨酸及缬氨酸和亮氨酸在这些树脂柱上的色谱分离 .结果表明 ,用两种方法得到的树脂对丙氨酸和缬氨酸的色谱分离性能基本相同 ;同时中性氨基酸与聚苯乙烯型强酸性阳离子交换树脂之间的作用包括离子作用和疏水作用 ,且二者之间存在协同作用 .树脂的交换容量较低时对中性氨基酸有更好的分离性能 相似文献
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聚丙烯酰胺型配体交换色谱载体的合成及分离氨基酸 总被引:1,自引:0,他引:1
合成了以亲水性聚丙烯酰胺为骨架的含有可与铜离子形成五元环螯合物配位基团的螯合树脂,这些树脂的Cu~(2+)络合物作为配体交换色谱载体对中性氨基酸进行了分离,并与2种已知螯合树脂的Cu~(2+)络合物作为载体的色谱行为作了比较。本文合成的树脂具有最好的分高性能,研究了色谱条件对分离的影响,讨论了色谱分离机制。 相似文献
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用粒径约0.5微米季胺化单分散交联聚苯乙烯胶乳与粒径为5~30微米磺化交联聚苯乙烯共聚物微球藉库伦力附聚得到了阴离子交换树脂微球,并对磺化深度,基球和胶乳的粒径比以及交联度等对阴离子交换树脂附聚微球色谱性能的影响进行了分析。用这种阴离子交换树脂附聚微球作为HPIC分离柱的填料;用自制的全多孔强阳离子交换树脂YSG-SO_3H为抑制柱填料,仅用10厘米长的分离柱在约27分钟内即可使F~-、Cl~-、No_2~-、HPO_4~-、Br~-、No_3~-、及SO_4~-等阴离子混合样按顺序全分离。 相似文献
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离子交换法分离乳酸的研究 总被引:4,自引:1,他引:4
本文对离子交换法分离乳酸的离子交换树脂、交换相平衡和交换动力学进行了研究,并在离子交换柱上测定了南开大学化工厂生产的201×4树脂的交换曲线。实验结果说明,用离子交换法分离发酵液中乳酸是可行的。 相似文献
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络合物离子色谱在微量金属分析中的新发展 总被引:3,自引:0,他引:3
评述了络合物IC(包括阳离子交换IC、配位IC、阴离子交换IC和离子对色谱)在微量金属分析中的新发展,以配位化学基本理论对金属络合物IC的保留行为和分离机理进行讨论,并对各种金属络合物IC技术和应用进行比较和对照。 相似文献
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Four neutral amino acids (Gly, Ala,.Val and Leu) were separated with ligand exchange resins. The separation capacity of the ligand exchange resins is compared with that of common ion exchange resins. The effects of eluent, column temperature, and central metal ions of the support on the separation are studied. The relationship between matrix structure of resins and their separation capacity is analysed. 相似文献
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Immunoglobulin G is an important plasma protein with many applications in therapeutics and diagnostics, which can be purified effectively by ion exchange chromatography. The ligand densities and pore properties of ion-exchange resins have significant effects on the separation behaviors of protein, however, the understandings are quite limited. In this work, with bovine immunoglobulin as the model IgG, the adsorption isotherms and adsorption kinetics were investigated systematically with series of diethylaminoethyl ion-exchange resins with different ligand densities and pore sizes. The Langmuir equation and pore diffusion model were used to fit the experimental data. The influences of ligand density and pore size on the saturated adsorption capacity, the dissociation constant and the effective diffusivity were discussed. The adsorption capacities increased with the increase of ligand density and the decrease of pore size, and an integrative parameter was proposed to describe the combined effects of ligand density and pore size. It was also found that the effective pore diffusion coefficient of the adsorption kinetics was influenced by pore sizes of resins, but was relatively independent on the ligand densities of resins. For a given protein, the ligand density and pore size should be optimized for improving the protein adsorption. 相似文献
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Chelating, sulfonic and cellulosic exchangers were compared in ligand exchange chromatography. Chelating resins retain metal ions the best, but have a low ligand binding capacity. Cellulosic exchangers show excessive metal leakage. Sulfonated polystyrene resins loaded with nickel ions give best results in most cases. Data are presented on ligand exchange chromatography of simple aliphatic amines, hydrazine and substituted hydrazines, purine and pyrimidine bases. 相似文献
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Abraham Warshawsky 《Separation & Purification Reviews》2013,42(1):37-48
The separation of platinum and palladium, by adsorption onto weak base anion exchange resins, Amberlite XE 299, or ionex (derived from Amberlite XE-305) from 1M hydrochloric acid, followed by ligand substitution with sulfur nucleophiles is described. In the thiourea system, cationic thiourea complexes are produced, which cannot be readsorbed into the resin. In the thiocyanate system the formation of Pd(SCN)4 2- is favored in both solution and polymer phases. The Pd(SCN)4 2-. readsorption into the anion exchange resin is the basis for the separation of platinum from palladium. 相似文献
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R. Shabana 《Journal of Radioanalytical and Nuclear Chemistry》1977,41(1-2):53-64
Anion exchange separation of Ga-In on Dowex 1 resins of 2, 4, 8, and 10 nominal cross-linkings, respectively, was investigated
at tracer loadings. The influence of temperature on the exchange reaction was also studied on the different resins. Separation
factors, plate heights, and resolutions for the two peaks were calculated from the elution curves. Best separations were achieved
with Dowex 1X4 due to the most promising values for both plate height and resolution. It was also found that the separation
factors as well as the plate heights decreased with growing temperature. 相似文献
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V. A. Ivanov V. D. Timofeevskaya V. I. Gorshkov N. V. Drozdova 《Journal of Radioanalytical and Nuclear Chemistry》1996,208(1):23-45
The evolution of ion exchange processes for separation and purification of substances by use of the influence of temperature both on the equilibrium and the dynamic properties of some ion exchange resins is discussed. Significant improvement of some ion exchange separations, diminishing of reagents consumption and waste quantities is achieved. In the same way, environmentally non-hazardous reagentless ion exchange methods of divalent and monovalent ionic mixtures separation are developed. This report is based on experimental studies of the equilibrium, dynamic and some other properties of ion exchange resins. 相似文献
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Ann Marie Hardin Chithkala Harinarayan Gunnar Malmquist Andreas Axén Robert van Reis 《Journal of chromatography. A》2009,1216(20):4366-4371
Dynamic binding capacity (DBC) of a monoclonal antibody on agarose based strong cation exchange resins is determined as a function of resin ligand density, apparent pore size of the base matrix, and protein charge. The maximum DBC is found to be unaffected by resin ligand density, apparent pore size, or protein charge within the tested range. The critical conductivity (conductivity at maximum DBC) is seen to vary with ligand density. It is hypothesized that the maximum DBC is determined by the effective size of the proteins and the proximity to which they can approach one another. Once a certain minimum resin ligand density is supplied, additional ligand is not beneficial in terms of resin capacity. Additional ligand can provide flexibility in designing ion exchange resins for a particular application as the critical conductivity could be matched to the feedstock conductivity and it may also affect the selectivity. 相似文献
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J. D. Navratil 《Journal of Radioanalytical and Nuclear Chemistry》1978,43(1):31-35
Pellicular, macroreticular and microreticular (gel-type) anion exchange resins were compared for the separation of plutonium
from nitric acid solutions of mixed plutonium-uranium. AS Pellionex SAX (pellicular resin) and Amberlite IRA-93 (weak base
macroreticular anion exchange resin) were found to have better uranium washing and plutonium eluting characteristics than
any of the resins tested. However, the capacity of the pellicular resin was much lower than the other resins. 相似文献
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Brandon Clark Dr. William A. Tarpeh 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(44):10099-10112
Extracting valuable products from wastewaters with nitrogen-selective adsorbents can offset energy-intensive ammonia production, rebalance the nitrogen cycle, and incentivize environmental remediation. Separating nitrogen (N) as ammonium from other wastewater cations (e.g., K+, Ca2+) presents a major challenge to N removal from wastewater and N recovery as high-purity products. High selectivity and capacity were achieved through ligand exchange of ammonia with ammine-complexing transition metals loaded onto polymeric cation exchange resins. Compared to commercial resins, metal–ligand exchange adsorbents exhibited higher ammonia removal capacity (8 mequiv g−1) and selectivity (N/K+ equilibrium selectivity of 10.1) in binary equimolar solutions. Considering optimal ammonia concentrations (200–300 mequiv L−1) and pH (9–10) for metal–ligand exchange, hydrolyzed urine was identified as a promising candidate for selective TAN recovery. However, divalent cation exchange increased transition metal elution and reduced ammonia adsorption. Ultimately, metal–ligand exchange adsorbents can advance nitrogen-selective separations from wastewaters. 相似文献