毛细管区带电泳中蛋白质吸附及其解决方法

本文评述毛细管区带电脉中蛋白质的吸附及解决办法，内容包括吸附的原因，通过缓冲溶液组成的变化、通过毛细管内壁改性、通过附加电场等方法降低吸附。对毛细管内壁电荷密度等方法降低吸附。尤其对毛细管内壁改性方法，改性对电渗流及分离效率的影响，改性的稳定性和重复性等进行了较为详细的阐述。     

新型共聚物涂层毛细管电泳柱及其分离蛋白质的研究

研究新型共聚物——ＺＢ系列表面键合剂在毛细管电泳中的应用。采用物理吸附的方法制备了ＺＢ－００４,ＺＢ－０１４,ＺＢ－０１６等３种涂层毛细管柱,在ｐＨ３～５范围内,３种涂层均能有效地降低管壁对蛋白质的吸附作用和电渗流,其中亲水性较弱的ＺＢ－００４涂层的分离性能最好。在ｐＨ＜５时,涂层具有较高的稳定性和良好的分析重复性,但在更高的ｐＨ值条件下,仍然存在着峰形畸变和电渗流迅速增加的现象。     

新型共聚物涂层毛细管电泳柱及其分离蛋白质的研究

 研究新型共聚物——ＺＢ系列表面键合剂在毛细管电泳中的应用。采用物理吸附的方法制备了ＺＢ－００４,ＺＢ－０１４,ＺＢ－０１６等３种涂层毛细管柱,在ｐＨ３～５范围内,３种涂层均能有效地降低管壁对蛋白质的吸附作用和电渗流,其中亲水性较弱的ＺＢ－００４涂层的分离性能最好。在ｐＨ＜５时,涂层具有较高的稳定性和良好的分析重复性,但在更高的ｐＨ值条件下,仍然存在着峰形畸变和电渗流迅速增加的现象。     

毛细管区带电泳中蛋白质的吸附及其解决方法

本文评述毛细管区带电泳中蛋白质的吸附及解决办法,内容包括吸附的原因,通过缓冲溶液组成的变化、通过毛细管内壁改性、通过附加电场等方法降低吸附。对毛细管内壁电荷密度等方法降低吸附。尤其对毛细管内壁改性方法,改性对电渗流及分离效率的影响,改性的稳定性和重复性等进行了较为详细的阐述。     

高效毛细管电泳在核酸、蛋白质分析中的新进展

高效毛细管电泳以其分离效率高，分析速度快，样品和试剂用量少，易于实现自动化等优点，在核酸、蛋白质等生物样品的分析方面发挥着重要的作用并具有巨大的潜力。本文介绍了近两年来高效毛细管电泳技术的进展，特别是PCR／CE、CE／MS以及电泳芯片技术等方面的新发展，并综述了高效毛细管电泳在核酸、蛋白质分析方面的应用，同时对其前景进行了展望。     

几种标准蛋白质的毛细管区带电泳的分离及其迁移行为

本文采用毛细管区带电泳分离了几种标准蛋白,研究了不同的清洗方式,不同ＰＨ的缓冲溶液等对迁移行为及分离的影响,并同时考察了迁移时间和相对迁移时间与操作电压的关系。     

毛细管电泳法表征离子液体与蛋白质的相互作用

基于毛细管区带电泳考察了不同离子液体的阳离子母核、烷基侧链碳原子数目和阴离子组成对牛血清白蛋白的影响,以及离子液体[C4mim]BF4与肌红蛋白、牛血清白蛋白、血红蛋白、牛凝血酶和转铁蛋白之间的相互作用。利用亲和毛细管电泳法比较了[C4mim]BF4与上述蛋白之间的相互作用,并计算得到结合常数Kb分别为1.24×107 L/mol,1.23×106 L/mol,5.75×104 L/mol和5.60×104 L/mol。结果表明,转铁蛋白、血红蛋白、肌红蛋白、牛血清白蛋白与离子液体的相互作用依次减弱,存在1～2个数量级的差异。结合毛细管区带电泳和亲和毛细管电泳模式可实现离子液体与多种蛋白质相互作用的定性与定量表征。     

奶制品中蛋白质测定的毛细管电泳法研究

本文利用毛细管电泳法研究了奶制品中蛋白成分的分离与测定。采用柠檬酸缓冲体系,对牛奶中的α-乳白蛋白(-αLa)、β-乳球蛋白(-βLg)、α-酷蛋白(-αCN)、β-酪蛋白(-βCN)、酪蛋白(-κCN)五种蛋白进行了很好的分离,其迁移时间和峰面积的相对标准偏差分别小于0.2%和5%;检出限分别为0.01、0.03、0.02、0.02、0.02 mg/mL;加标回收率范围为84%~103%。应用该法测定了多种奶制品中的蛋白质含量。本法简便、快速、准确,为牛奶及奶制品质量的监控提供了一种新的手段。     

直接鉴别延胡索及其伪品的FTIR-主成分分析法研究

采用傅里叶变换红外光谱直接测定法测定了延胡索及其伪品齿瓣元胡、东北延胡索、土元胡、新疆延胡索及夏天无的傅里叶变换红外光谱,采用主成分析法比较了正伪品之间的差异程度.结果表明基于傅里叶变换红外光谱的主成分分析在反映同属不同种植物化学组成差异程度上具有应用价值.     

毛细管电泳中的电渗及其控制

渗在毛细管电泳中与淌度生容量因子同等重要，它决定或控制电泳的分离模式、分离过程及其分离生现性等。本文对电渗的作用，控制方法进行了系统讨论，并介绍了此方面的研究进展及有关的问题。     

Chemical constituents from the fruits of Psoralea corylifolia and their protective effects on ionising radiation injury

Two new flavonoids, corylifol F (1) and corylifol G (2), together with 19 known compounds, were isolated from the fruits of Psoralea corylifolia L.. The structures of these compounds were determined by interpretation of spectroscopic data and comparison with literature properties. The radioprotective effects of the isolated compounds against ionising radiation damage were also evaluated in vitro. The results showed that corylifol A exhibited radioprotective effects in both HBL-100 and MCF-7 cells, while psoralen, isopsoralen, corylifol C and bakuchiol showed obvious selective action to protect HBL-100 cells against damage caused by ionising radiation.     

Exploration of Antimicrobial Ingredients in Psoralea corylifolia L. Seed and Related Mechanism against Methicillin-Resistant Staphylococcus aureus

With the abuse of antibiotics, bacterial antibiotic resistance is becoming a major public healthcare issue. Natural plants, especially traditional Chinese herbal medicines, which have antibacterial activity, are important sources for discovering potential bacteriostatic agents. This study aimed to develop a fast and reliable method for screening out antimicrobial compounds targeting the MRSA membrane from Psoralea corylifolia Linn. seed. A UPLC-MS/MS method was applied to identify the prenylated flavonoids in major fractions from the extracts of Psoralea corylifolia Linn. seed. The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of different fractions and compounds. The morphological and ultrastructural changes of MRSA were determined by scanning electron microscopy (SEM). The membrane-targeting mechanism of the active ingredients was explored by membrane integrity assays, membrane fluidity assays, membrane potential assays, ATP, and ROS determination. We identified eight prenylated flavonoids in Psoralea corylifolia Linn. seed. The antibacterial activity and mechanism studies showed that this type of compound has a unique destructive effect on MRSA cell membranes and does not result in drug resistance. The results revealed that prenylated flavonoids in Psoralea corylifolia Linn. seeds are promising candidates for the development of novel antibiotic agents to combat MRSA-associated infections.     

超临界流体萃取法测定补骨脂中的主要成分

 采用超临界流体萃取(SFE)技术和CGC技术测定了 中药补骨脂中的补骨脂素和异补骨脂素。对超临界流体萃取过程中影响萃取效率的主要因素 采用正交设计法和方差分析法进行了考察,确定了主效应和适宜的操作条件。与传统的萃取 法比较,SFE 具有经济、快速、简便、选择性好、环境污染小等优点。     

Determination of Taurine in Lycium Barbarum L. and Other Foods by Capillary Electrophoresis with Electrochemical Detection

A method based on capillary electrophoresis (CE) with electrochemical detection (ED) was developed for the determination of taurine in Lycium Barbarum L., LIPOVIYAN beverage and milk powder. The effects of some important factors such as the acidity of the running buffer, separation voltage, injection time, and applied potential to working electrode were investigated. Operated in a wall‐jet configuration, a 300 μm diameter carbon‐disk electrode was used as the working electrode, which exhibits good responses at +1.05 V (vs. SCE) for taurine. Excellent linearity was obtained in the concentration range from 5.0×10^?4 mol/L to 5.0×10^?6 mol/L. The detection limit (S/N=3) was 1.0×10^?7 mol/L. This proposed method has been successfully applied to analyze the actual samples with satisfactory assay results.     

豚草聚合酶链反应产物的高效毛细管电泳检测

利用PE270A－HT型高效毛细管电泳仪,采用无胶筛分电泳,以羟丙基甲基纤维素（HPMC）为筛分介质,对三裂叶豚草（Ambrosia trifida L.)的聚合酶链反应（PCR）产物进行了分离分析,显示了高效、快速、无污染的毛细管电泳技术在植物DNA片段多态性分析方面的巨大的潜力。     

Determination of Active Ingredients of Origanum Vulgare L. and Its Medicinal Preparations by Capillary Electrophoresis with Electrochemical Detection

Abstract

A method based on capillary electrophoresis with electrochemical detection (CE‐ED) has been developed for the first time for the separation and determination of isovanillic acid, vanillic acid, quercetin, rosmarinic acid, caffeic acid, and protocatechuic acid in Origanum vulgare L. and its medicinal preparations. The effects of working electrode potential, pH level, concentration of running buffer, separation voltage, and injection time on CE‐ED were investigated. Under the optimum conditions, the analytes could be separated in a 50 mmol L^?1 borate buffer (pH 8.7) within 21 min. A 300‐µm diameter carbon disk electrode has a good response at +0.95 V (vs. SCE) for all analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) ranging from 4×10^?8 g mL^?1 to 2×10^?7 g mL^?1 for the analytes. The method has been successfully applied to the analysis of real sample, with satisfactory results.     

藏药蕨麻多糖水解单糖的毛细管区带电泳分离研究

以1-萘基-3-甲基-5-吡唑啉酮(NMP)为柱前衍生试剂,探讨了毛细管区带电泳模式下对藏药蕨麻多糖水解液中单糖的分离条件。实验采用58.5 cm×50μm i.d.毛细管(有效长度50 cm),55 mmol/L硼酸盐缓冲溶液(pH=9.46),柱温20℃,分离电压22 kV,进样10 s。该法不加任何添加剂,9种单糖可高效、快速基线分离,实现了对藏药蕨麻多糖水解液中单糖的分离和定量分析,结果令人满意。     

Simultaneous determination of phytoestrogens in different medicinal parts of Sophora japonica L. by capillary electrophoresis with electrochemical detection

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of phytoestrogens from the pericarps and seeds of Sophora japonica L. in this work. Genistin, genistein, rutin, kaempferol and quercetin are important bioactive constituents in these plants. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on the CE-ED procedure were investigated. Under the optimum conditions, the five analytes could be well separated within 18 min in a 75 cm length capillary (i.d. 25 microm) at the separation voltage of 16 kV in a 50 mmol L(-1) borax running buffer (pH 9.0). A 300 microm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at the potential of +950 mV (vs SCE). Detection limits (S/N = 3) ranged from 1.1 x 10(-7) to 2.8 x 10(-7) g mL(-1) for all fi ve analytes. This method was successfully used to analyse dried Flos sophorae immaturus, pericarps and seeds of dried Fructus sophorae after a relatively simple extraction procedure, and the assay results were satisfactory.     

毛细管电泳-电化学检测法测定中药杠版归中的活性成分

采用毛细管电泳-电化学检测法（CE-ED）同时分离测定了中药杠板归中的阿魏酸、香草酸、槲皮素、咖啡酸、原儿茶酸等主要生物活性成分的含量。考察了工作电极电位、运行缓冲液的pH值和浓度、分离电压和进样时间等实验参数对实验结果的影响。在优化的实验条件下,以直径300 μm的碳圆盘电极为工作电极,检测电位为＋0.95V(vs. SCE),在10mmol/L磷酸盐（pH 9.2）的运行缓冲溶液中,五个分析物能够在17min内实现很好的基线分离,被测物浓度与峰电流在3个数量级呈良好的线性,检测限（S/N＝3）范围从7.1×10-8 到 9.3×10-8g mL-1。该方法已应用于实际样品的分析,样品处理简单,获得了令人满意的结果。     

Evaluation of the influence of salt processing on pharmacokinetics of psoralen and isopsoralen in Psoralea corylifolia L.

A sensitive, specific and rapid ultra‐high‐pressure liquid chromatography tandem mass spectrometry (UHPLC‐MS/MS) method has been developed to investigate pharmacokinetic properties of psoralen and isopsoralen, two compounds isolated from raw/salt‐processed fruit of Psoralea corylifolia L. UHPLC‐MS/MS was used with positive ion electrospray. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous solution and a gradient elution program at flow rate of 0.3 mL/min was applied. Multiple reaction monitoring mode was used for the quantification of psoralen, isopsoralen ([M + H]⁺ m/z 187.0 → m/z 131.0) and scoparone (m/z 207.0 → m/z 151.1). Scoparone served as an internal standard. The method was fully validated for its sensitivity, selectivity, stability, matrix effect and extraction recovery. The obtained results showed that salt‐processed Buguzhi significantly promoted the absorption of psoralen and isopsoralen, and increased the bioavailability of these compounds. Copyright © 2015 John Wiley & Sons, Ltd.