毛细管电泳指纹图谱及毛细管电泳-质谱联用在中药质量控制中的作用

以作者多年从事毛细管电泳、毛细管电泳-质谱联用及其指纹图谱对中药的研究成果为基础,介绍了毛细管电泳实验条件的优化方法、毛细管电泳指纹图谱的研究方法与评价方法及其在中药质量控制中的作用。     

α-干扰素的液相色谱/毛细管电泳两维分离分析

用亲和色谱/反相色谱、亲和色谱/毛细管电泳和凝胶色谱/毛细管电泳等两维系统, 对白细胞提取液中的α-干扰素进行分离分析, 并对结果进行比较,充分肯定了亲和色谱/毛细管电泳联用在蛋白质类药物制备纯化中的显著作用,以及毛细管电泳作为此类药物分析工具的可能性。     

毛细管电泳中的毛细管区段灌注及其相关技术

毛细管区段灌注是一种随手性毛细管电泳、亲和毛细管电泳和胶束电动力色谱-质谱发展而产生的实验技术。由于它能消除采用以上方法时遇到的缓冲溶液对检测的影响。因此引起人们的关注。本文对区段灌注技术的原理,区段灌注技术在手性毛细管电泳、亲和毛细管电泳和胶束电动力色谱中的应用,以及目前区段灌注技术存在的一些问题进行了评述。本文还对由区段灌注技术发展而来的逆流毛细管电泳、液相预柱毛细管电泳、多步配体进样等实验技术进行了简介。     

毛细管电泳在火炸药研究中的应用

近年来,毛细管电泳以其高效、快速、微量的特点,在火炸药分析领域被广泛应用并迅速发展。本文从毛细管区带电泳、胶束电动毛细管色谱、毛细管电色谱、芯片毛细管电泳、微乳毛细管电动色谱这五种毛细管电泳的模式出发,结合本实验室的最新研究进展,综述毛细管电泳在火炸药研究中的应用。     

芯片毛细管电泳中组分的迁移行为及其特征

在自组装的芯片毛细管电泳-激光诱导荧光检测装置上,以单个染料和一组荧光素异硫氰酸酯(FITC)标记的氨基酸为对象,研究了芯片毛细管电泳与传统毛细管电泳之间的差别,考察了玻璃芯片上微通道内的伏安特性以及抑制电压、进样方式和检测点的位置等对芯片毛细管电泳分离分析的影响,特别注意到了其有别于传统毛细管电泳的各种行为特征.     

单分子毛细管电泳

对室温下液流中的单分子光学检测技术的基本原理、主要方法以及它在生物学中的应用做了综述。重点强调了毛细管电泳与单分子检测相结合-单分子毛细管电泳在分析科学中战略上的重要性，并展望了单分子光学检测技术为特征的单分子毛细管电泳在分析化学中的应用前景。     

芯片毛细管理电泳中组分的迁移行为及其特征

在自组装的芯片毛细管电泳-激光透导荧光检测装置上，以单个染料和一组荧光素异硫氰酸酯（FITC）标记的氨基酸为对象。研究了芯片毛细管电泳与传统毛细管电泳之间的差别，考察了玻璃芯片上微通道内的伏安特性以及抑制电压、进样方式和检测点的位置等对芯片毛细管电泳分离分析的影响，特别注意到了其有别于传统毛细管电泳的各种行为特征。     

毛细管电泳应用于食品中农药残留分析的晚近进展

对2000-2008年毛细管电泳在食品农药残留分析中的应用进展进行综述,共分为三部分:第一部分介绍了毛细管电泳在农药分析中的一些主要分离模式和检测方法;第二部分介绍了与毛细管电泳联用的主要样品预处理方法;第三部分评述了近年来应用于食品中农药残留分析的各种检测方法和毛细管电泳分离模式.并探讨了这一研究领域的未来发展趋势.引用文献56篇.     

毛细管电泳涂层柱技术的进展

毛细管电泳涂层柱是解决蛋白质在毛细管壁吸附的最有效的方法。较为系统地综述了毛细管电泳涂层柱的几种制作方法,指出了毛细管电泳涂层柱（包括毛细管电色谱柱）的发展趋势,３９篇。     

无电渗流的毛细管电泳

无电渗流毛细管电泳是毛细管电泳的一种新技术。本文对基产生条件及其在毛细管区带电泳与胶束电动毛细管色谱中的分离特点进行了详细介绍。     

二氧化碳和丙烯直接合成甲基丙烯酸的NiPMo/TiO2催化剂的研究

用溶胶-凝胶法以磷钼酸(MPA)的镍盐溶液水解钛酸四丁酯制备了NiPMo/TiO2催化剂.使用ICP、 XRD、 TG-DTA、 IR、 TPD-MS和微反应技术研究了催化剂的化学组成、热稳定性、化学吸附性质和催化反应性能.杂多钼酸盐与TiO2通过O2-在TiO2表面发生了键合.在623 K下,杂多阴离子仍保持原有的Keggin结构.CO2在Lewis酸位Ni(Ⅱ)和Lewis碱位Ni-O-Mo的桥氧协同作用下生成CO2卧式吸附态Ni(Ⅱ)←O-(CO)←(O--Ni).丙烯有多种吸附态在催化剂上吸附.在563 K、 1 MPa和空速1500 h-1的反应条件下,丙烯的摩尔转化率为3.2%,产物MAA选择性为95%.     

Synthesis of 1-benzyloxypyrazin-2(1H)-one derivatives

Different approaches for the synthesis of 1-benzyloxypyrazin-2(1H)-one derivatives from simple amino acids have been investigated. A library of 33 precursors for the preparation of N-hydroxy pyrazinones was obtained in moderate to good yields.     

Toward new camptothecins. Part 6: Synthesis of crucial ketones and their use in Friedländer reaction

In the context of the preparation of camptothecin and luotonin A analogs, the synthesis of some key keto-precursors and their use in Friedländer condensation are described. This paper also focuses on the stability of these keto intermediates and emphasizes the major differences between indolizinones and pyrroloquinazolinones series. Noteworthy is also the report of some original structures isolated as by-products of some experiments.     

CoFe2O4自形成磁性液体场致结构化对磁化的影响

The Langevin paramagnetic theory can’t describe the relation between magnetization of ferrofluids and applied magnetic field. The structuralization of ferrofluids, which is considered the main influence factor of the magnetization, is regarded. The part of magnetization works is deposited when the structure is forming. This action influences the magnetization of ferrofluids directly or indirectly. On the base of the “compressing” model, the Langevin function that usually describes the magnetization of ferrofluid is modified, and a well-fitted curve is obtained. An equation of the relation between the equivalent volume fraction after being “compressed” and the intensity of magnetic field is discovered, which approximately describes the process of magnetization. The relation between the approximate initial susceptibility and the volume fraction can be obtained from modified formula.     

Highly regioselective Buchwald–Hartwig amination at C-2 of 2,4-dichloropyridine enabling a novel approach to 2,4-bisanilinopyridine (BAPyd) libraries

The highly regioselective Buchwald–Hartwig amination at C-2 of the cheap and readily accessible reagent, 2,4-dichloropyridine with a range of anilines and heterocyclic amines is described. This new methodology is robust and provides a facile access to 4-chloro-N-phenylpyridin-2-amines on 0.25 mol scale. These intermediates undergo a further Buchwald–Hartwig amination at higher temperature to enable rapid exploration of the chemical space at C-4 and to provide a library of 2,4-bisaminopyridines.     

KMnO4-mediated oxidative CN bond cleavage of tertiary amines: Synthesis of amides and sulfonamides

KMnO₄-mediated oxidative CN bond cleavage of tertiary amines producing secondary amine was introduced, which was trapped by electrophiles (acyl chloride and sulfonyl chloride) to form amides and sulfonamides. The reaction could take place at mild condition, tolerating a wide range of function groups and affording products in moderate to excellent yields.     

Chemistry of heterocyclic compounds at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences, over 50 years (Review)

The review contains a concise historical account and information on the most significant researches undertaken by the staff at the A. E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch of the Russian Academy of Sciences on the Chemistry of Heterocyclic Compounds. Dedicated to Academician of the Russian Academy of Sciences B. A. Trofimov on his 70th jubilee. Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 10, pp. 1443–1502, October, 2008.     

A general and convenient synthesis of 4-(tosylmethyl)semicarbazones and their use in amidoalkylation of hydrogen,heteroatom, and carbon nucleophiles

A general synthesis of previously unknown semicarbazone-based α-amidoalkylating reagents, 4-(tosylmethyl)semicarbazones, has been developed. The synthesis involved three-component condensation of semicarbazones of aliphatic or aromatic aldehydes with the same or other aldehydes and p-toluenesulfinic acid. The scope and limitations of this reaction were investigated. The compounds obtained were demonstrated to be an efficient α-(4-semicarbazono)alkylating agents. They were reacted with H- (sodium borohydride), O- (sodium methylate), S- (sodium phenylthiolate), N- (pyrrolidine, sodium succinimide), P- (trialkyl phosphites), and C-nucleophiles (sodium diethyl malonate) to give the corresponding products of the tosyl group substitution, 4-substituted semicarbazones, including analogues of nitrofurazone. Among the prepared compounds tested in vitro for antibacterial and antifungal activity, three nitrofuryl-containing semicarbazones exhibited high biological activities with minimum inhibitory concentration (MIC) values of 8–32 μg/mL.     

Sulfur-directed metal-free and regiospecific methyl C(sp3)–H imidation of thioanisoles

Regiospecific and direct imidation of the methyl C(sp³)–H bond of thioanisoles is realized under mild and metal-free conditions with N-fluorobis(benzenesulfonyl)imide as an oxidant and nitrogen source. Proposed mechanism suggests that thionium ion intermediates and a Pummerer-type reaction are involved. The imidation has advantages such as high step-economy, excellent functionality tolerance, and regiospecificity, giving structurally diverse imidation products.     

Synthesis of novel chiral bidentate hydroxyalkyl-N-heterocyclic carbene ligands and their application in palladium-catalyzed Mizoroki–Heck couplings and asymmetric addition of diethylzinc to benzaldehyde

A small library of new chiral bidentate hydroxyalkyl-imidazolium salts 1 is conveniently synthesized on multi-gram scale from inexpensive and commercially available chiral pool amino acids. The corresponding carbenes, generated by deprotonation of imidazolium salts 1, in combination with palladium(II) chloride were tested in the Mizoroki–Heck coupling reaction. The most significant results in terms of yields and reactivities were achieved with low catalyst loading. The catalytic activities of these imidazolium salts were also investigated in the asymmetric addition of diethylzinc to benzaldehyde. The use of MgO nanoparticles as an additive in conjunction with these ligands played a crucial role in increasing the efficiency of these reactions.