2020年毛细管电泳技术年度回顾

该文为2020年毛细管电泳(capillary electrophoresis,CE)技术年度回顾.归纳总结了以"capillary electro-phoresis-mass spectrometry"或"capillary isoelectric focusing"或"micellar electrokinetic...     

2021年毛细管电泳技术年度回顾

该文为2021年毛细管电泳(capillary electrophoresis, CE)技术年度回顾。归纳总结了以“capillary electrophoresis-mass spectrometry”或“capillary isoelectric focusing”或“micellar electrokinetic chromatography”或“capillary electrophoresis”为关键词在ISI Web of Science数据库中进行主题检索(排除“capillary electrochromatography”“microchip”和“capillary monolithic column”)得到的2021年CE技术相关研究论文291篇,以及中文期刊《色谱》和《分析化学》中相关研究论文9篇。重点介绍了影响因子(IF)≥10.0的Coordination Chemistry Reviews, Angewandte Chemie-International Edition, Nature Protocols, TrAC-Trends in Analytical Chemistry, Signal Transduction and Targeted Therapy发表的7篇论文;以及影响因子5~10之间的代表性期刊Analytical Chemistry, Analytica Chimica Acta, Talanta和Food Chemistry的42篇论文;对影响因子小于5但CE技术报道较为集中的Journal of Chromatography A和Electrophoresis,国内重要的中文期刊《色谱》和《分析化学》中的代表性工作进行了概述。该文根据国际通用学术水平评价指标之一的影响因子选择期刊,结合期刊发表CE论文代表性工作进行介绍,便于读者快速了解毛细管电泳技术在过去一年的重要研究进展。     

Method Development of Enantiomer Separations by Affinity Capillary Electrophoresis,Cyclodextrin Electrokinetic Chromatography and Capillary Electrophoresis-Mass Spectrometry

 Capillary electrophoresis (CE) has become a powerful tool for enantiomer separations during the last decade. Since 1993, the author has investigated enantiomer separations by affinity capillary electrophoresis (affinity CE) with some proteins and by cyclodextrin electrokinetic chromatography (CDEKC) with some charged cyclodextrins (CDs). Many successful enantiomer separations are demonstrated from our study in this review article. In the enantiomer separations by affinity CE, the deterioration of detection     

On-Line Multichannel Raman Spectroscopic Detection System For Capillary Zone Electrophoresis

Detection and peak identification are key factors in developing CE (capillary electrophoresis) methodology. They have become hotspots for many reasons. The most obvious one lies in the revealing of unknown species in the application of CE to biological samples. CE-MS and CE-NMR have since been developed. CE-Raman spectroscopy was also reported1. As compared to the former two methods, the Raman spectroscopy method is highly attractive because it has the potential to provide high-informat…     

Comparison of Carboxylic and Hydroxycarboxylic Acids as Complexing Agents for Transition Metal Separation with Nonaqueous Capillary Electrophoresis

Many papers have been published on the analysis of metal cations by capillary electrophoresis (CE), for example, lanthanide, transition metal, alkali, and alkaline earth metal ions. Separations of metal ions are based on the differences in their electrophoretic mobilities. Since most     

Analysis of Glutamic Acid in Cerebrospinal Fluid by Capillary Electrophoresis with High Frequency Conductivity Detection

Glutamic acid(Glu)is the most important amino acid in the nerve center system1.It isessential to detect Glu in cerebrospinal fluids(CSF),because the level of Glu in CSF hasimplicated several diseases such as metabolic diseases or central nervous systemdisorders2.The measurement of Glu in CSF had been performed by high performanceliquid chromatography(HPLC)and capillary electrophoresis(CE)with inducedfluorescence detection3-7.Since Glu shows no fluorescence,different reagents had beenus…     

On-line Separation and Detection of Peptides by Capillary Electrophoresis / Electrospray FT-ICR-MS

Recently, capillary electrophoresis/electrospray ionization mass spectrometry (CE/ESI- MS) has been rapidly developed as a powerful analytical tool for charged species ranged from small molecules such as carboxylic acids1, phenolic compounds2, metal species3, tetramines4, herbicides5, drugs and drug metabolites6 to peptides and proteins7, 8. The ESI mode has proven to be sensitive, versatile and relatively easy to use in combination with CE. CE confers rapid analysis and efficient separ…     

Determination of N-Methylcarbamate Pesticides in Vegetables by Solid-phase Extraction and Pressurized Capillary Electrochromatography

Introduction Capillary electrochromatography(CEC) is a hybrid technique that couples the good selectivity of high-performance liquid chromatography(HPLC) and the high separation efficiency of capillary electrophoresis(CE).Both charged and uncharged compou…     

Enantiomeric Separation of Antidepressant Trimipramine by Capillary Electrophoresis Combined with Electrochemiluminescence Detection in Aqueous-organic Media

The antidepressant trimipramine(Tri) enantiomers were successfully separated by capillary electrophoresis(CE) coupled with electrochemiluminescence(ECL) detection in aqueous-organic media. A dual cyclodextrin(CD) system combining β-CD and hydroxypropyl-β-cyclodextrin(HP-β-CD) was used as chiral selector. Acetonitrile(ACN) was added to the running buffer to improve the separation efficiency, detection sensitivity and repeatability. The method was also successfully applied to the chiral separation of Tri in s...     

A Novel Sieving Medium for Separation of DNA Fragments- Poly(acrylamide-dimethylacrylamide)

IntroductionCapillary electrophoresis (CE) in polymer solution has become an attractive alternative to slab gel electrophoresis for DNA analysis1, 2. This technique has been successfully applied for mutation detection, genotyping, DNA sequencing and gene expression3-7. In order to perform DNA analysis, the inner surface of the capillary must be modified, usually by covalent bonding of hydrophilic polymers. However, the covalent coating will increase the cost and often causes problems rela…     

Capillary electrophoresis-chemiluminecence detection of levodopa and benserazide in Medopar tablet

A novel method for the simultaneous determination of benserazide and levodopa using capillary electrophoresis(CE)- chemiluminescence(CL)has been developed.Under the optimal conditions,the detection limits(S/N=3)were 1.85μg/mL for benserazide and 0.12μg/mL for levodopa.This method was successfully applied to the determination of benserazide and levodopa in Medopar tablet,the results showed that the detected values are in accordance with those by official methods.     

A Compact System of Capacitively Coupled Contactless Conductivity Detection Based on the Square Wave Excitation Signal for Capillary Electrophoresis

Recently, a detector for capillary electrophoresis (CE) termed capacitively coupled contactless conductivity detection (C4D) has been greatly developed. This detector was firstly reported by Zemann and do Lago groups1,2. Investigations have been focused o…     

A Capillary Electrophoresis Detection Scheme for Water-soluble Vitamins Based on Luminol-BrO^- Chemilumlinescence System

A novel chemiluminescence detection scheme has been developed for detecting water-soluble vitamins following capillary electrophoresis(CE) separation.This detection was based on the inhibitory effect of vitamins on the CL reaction between luminol and BrO^- in basic aqueous solution.Detection of vitamins was accomplished with a borate-based background electrolyte at pH9.2.The luminol was used as a component of the separation carrier electrolytc.     

Rapid separation of acetophenone and its monohydroxy isomers by capillary electrophoresis

This work describes the development of a capillary electrophoresis(CE)method for the simultaneous separation of acetophenone(AP),2-hydroxyacetophenone(2-HAP),3-hydroxyacetophenone(3-HAP)and 4-hydroxyacetophenone(4-HAP)in synthetic mixtures using 10 mmol/L of sodium tetraborate buffer(pH 9.5).The aim of this work is to demonstrate the effectiveness of CE to separate AP and its monohydroxy isomers and to defne how the separations are affected by buffers,buffer pH,sample matrices and separation voltage.This method was successfully used for the trace level separation and determination of 2-HAP,3-HAP and 4-HAP in synthetic mixture and 4-HAP in spiked plasma samples.     

Determination of Enantiomeric Purity of ν-Pyrrolidinyl Phenylpropanol by Capillary Electrophoresis Using β-Cyclodextrin Polymer as Chiral Selector

Determining the enantiomeric excess value of novel chiral compounds is of great importance in asymmetry synthesis. NMR1, HPLC and GC are the ordinary methods applied in this work. In recent years, capillary electrophoresis has been successfully used in chiral separation2. In this paper, we separated the enantiomer of (-pyrrolidinyl phenylpropanol (Figure 1) by capillary electrophoresis using (-cyclodextrin polymer as chiral selector. According to the ratio of peak area with migration time,…     

Chiral Separations by Ligand-Exchange Micellar Electrokinetic

The separation of enantiomers is of great importance in biology, pharmaceutics, agriculture and environment. The different separation modes ( i. e. capillary zone electrophoresis ( CZE ), micellar electrokinetic chromatography ( MEKC ), capillary electrochromatography ( CEC ), etc )     

Determination of ephedrine and codeine in human urine by cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography

A sensitive method for the determination of ephedrine and codeine in human urine by capillary electrophoresis(CE)was described.In order to improve the sensitivity,two online concentration techniques including cation-selective exhaustive injection (CSEI)and sweeping micellar electrokinetic chromatography(sweeping-MEKC)were used.Under the optimum conditions,the detection limits(S/N=3)were 0.10μg/L for ephedrine and 0.80μg/L for codeine.This method was successfully applied to real urine sample analysis.     

Separation of chiral drugs with β-CD phosphate by capillary electrophoresis

In capillary electrophoresis (CE) chiral separation is accomplished by adding suitableselector in the running electrolyte"'. The type of selector is of primary importance forachieving successful resolution. Selector concentration has considerable influence aswell3'. Charged cyclodextrins were first introduced for chiral separation of aminoacidss and used for enantioseparation of drugs by Terabe group'. Many kinds ofcharged CDs are now commercially available7-11. The charged CD commonly use…     

Optimized Separation of Pharmacologically Active Xanthones from Secuidaca inappendiculata by Capillary Electrophoresis

A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers,addition of sulfated β-CD,applied voltage and column temperature.Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH9.5) and 10 mmol/L sulfated β-CD.     

Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection

This work described a sensitive method for determination of metoprolol in rabbit plasma.The method involved purification by ultrafiltration,derivatization with fluorescein isothiocyanate,determination by capillary electrophoresis(CE) coupled with laser-induced fluorescence(LIF) detector.Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol in experimental condition.The assay had a wide range(2.0-500 ng/mL) of linearity and a detection limit of 0.8 ng/mL.The intra- and inter-day precisions were satisfactory with relative standard deviation(RSD) less than 10.0%and accuracy within 10.0%.This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.