Biogenic Silver Nanoparticles from Two Varieties of Agaricus bisporus and Their Antibacterial Activity

Agaricus bisporus, the most widely cultivated mushroom, is safe to eat and enriched with protein and secondary metabolites. We prepared silver nanoparticles (AgNPs) from two varieties of A. bisporus and tested their antibacterial activity The synthesized AgNPs were initially confirmed by UV-Vis spectroscopy peaks at 420 and 430 nm for white and brown mushrooms AgNPs, respectively. AgNPs were further characterized by zeta sizer, transmission electronic microscopy (TEM), Fourier transform infrared (FTIR), and energy-dispersive X-ray spectroscopy (EDX) prior to antibacterial activity by the well diffusion method against six bacterial strains which include Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. TEM results revealed a spherical shape with an average diameter of about 11 nm in the white mushroom extract and 5 nm in the brown mushroom extract. The presence of elemental silver in the prepared AgNPs was confirmed by EDS. The IR spectrum of the extract confirmed the presence of phenols, flavonoids, carboxylic, or amide groups which aided in the reduction and capping of synthesized AgNPs. The AgNPs from both extracts showed almost the same results; however, nanoparticles prepared from brown mushrooms were smaller in size with strong antibacterial activity.     

Synthesis of Silver Nanoparticles from Extracts of Wild Ginger (Zingiber zerumbet) with Antibacterial Activity against Selective Multidrug Resistant Oral Bacteria

Antibiotic resistance rate is rising worldwide. Silver nanoparticles (AgNPs) are potent for fighting antimicrobial resistance (AMR), independently or synergistically. The purpose of this study was to prepare AgNPs using wild ginger extracts and to evaluate the antibacterial efficacy of these AgNPs against multidrug-resistant (MDR) Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. AgNPs were synthesized using wild ginger extracts at room temperature through different parameters for optimization, i.e., pH and variable molar concentration. Synthesis of AgNPs was confirmed by UV/visible spectroscopy and further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy analysis (EDXA), and Fourier-transform infrared spectroscopy (FTIR). Disc and agar well diffusion techniques were utilized to determine the in vitro antibacterial activity of plant extracts and AgNPs. The surface plasmon resonance peaks in absorption spectra for silver suspension showed the absorption maxima in the range of 400–420 nm. Functional biomolecules such as N–H, C–H, O–H, C–O, and C–O–C were present in Zingiber zerumbet (Z. zerumbet) (aqueous and organic extracts) responsible for the AgNP formation characterized by FTIR. The crystalline structure of ZZAE-AgCl-NPs and ZZEE-AgCl-NPs was displayed in the XRD analysis. SEM analysis revealed the surface morphology. The EDXA analysis also confirmed the element of silver. It was revealed that AgNPs were seemingly spherical in morphology. The biosynthesized AgNPs exhibited complete antibacterial activity against the tested MDR bacterial strains. This study indicates that AgNPs of wild ginger extracts exhibit potent antibacterial activity against MDR bacterial strains.     

Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity

The silver nanoparticles (AgNPs) synthesized using hot water olive leaf extracts (OLE) as reducing and stabilizing agent are reported and evaluated for antibacterial activity against drug resistant bacterial isolates. The effect of extract concentration, contact time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles are investigated. The data revealed that the rate of formation of the nanosilver increased significantly in the basic medium with increasing temperature. The nature of AgNPs synthesized was analyzed by UV–vis spectroscopy, X-ray diffraction, scanning electron microscopy and thermal gravimetric analysis (TGA). The silver nanoparticles were with an average size of 20–25 nm and mostly spherical. The antibacterial potential of synthesized AgNPs was compared with that of aqueous OLE by well diffusion method. The AgNPs at 0.03–0.07 mg/ml concentration significantly inhibited bacterial growth against multi drug resistant Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). This study revealed that the aqueous olive leaf extract has no effect at the concentrations used for preparation of the Ag nanoparticles. Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future.     

Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose

The focus of the study is to compare the antibacterial efficacy of silver nanoparticles (AgNPs) fabricated by exploiting biological (a mangrove plant, Rhizophora apiculata) and chemical means (Glucose). The synthesized nanoparticles were characterised using UV-visible absorption spectrophotometry (UV-vis), Fourier transform Infra-red Spectroscopy (FTIR) and Transmission electron microscopy (TEM). Biologically synthesized silver nanoparticles (BAgNPs) were observed at 423 nm with particle sizes of 19-42 nm. The chemically synthesized silver nanoparticles (CAgNPs) showed a maximum peak at 422 nm with particle sizes of 13-19 nm. An obvious superiority of the antibacterial potency of BAgNPs compared to the CAgNPs as denoted by the zone of inhibition (ZoI) was noted when the nanoparticles were treated against seven different Microbial Type Culture Collection (MTCC) strains. The current study therefore elucidates that the synthesized AgNPs were efficient against the bacterial strains tested.     

Green synthesis of silver nanoparticles by bloom forming marine microalgae Trichodesmium erythraeum and its applications in antioxidant,drug-resistant bacteria,and cytotoxicity activity

In the present study, we developed an eco-friendly method of stable silver nanoparticles (AgNPs) production using the aqueous extract of Trichodesmium erythraeum. The biosynthesized AgNPs were characterized using UV–Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Energy-Dispersive X-ray (EDX), and X-ray diffraction (XRD). The results affirmed that synthesized AgNPs were crystalline in nature, cubical in shape, and the average size of T. erythraeum silver nanoparticles (TENPs) was 26.5 nm. The antioxidant potential of synthesized AgNPs (500 µg/ml) was 77.01 ± 0.17% in DPPH, 67.5 ± 0.22% in Deoxy-ribose, 52.77 ± 0.42% in ABTS and 88.12 ± 0.26% in nitric oxide radical scavenging assays. The antibacterial results showed excellent inhibition against the clinical strains (Staphylococcus aureus and Proteus mirabilis) and drug-resistant bacterial strains such as E. coli (Amikacin^R), S. aureus (Tetracycline^R) and S. pneumoniae (Penicillin^R). The maximum anti-proliferative effect of TENPs was seen using 50 µg concentration against He La and MCF-7 cell lines, and IC₅₀ values were 25.0 ± 0.50 µg/ml and 30.0 ± 0.50 µg/ml, respectively, at 24 h.     

Antibacterial Activity and Synergistic Effect of Biosynthesized AgNPs with Antibiotics Against Multidrug-Resistant Biofilm-Forming Coagulase-Negative Staphylococci Isolated from Clinical Samples

Silver nanoparticles form promising template for designing antimicrobial agents against drug resistant pathogenic microorganisms. Thus, the development of a reliable green approach for the synthesis of nanoparticles is an important aspect of current nanotechnology research. In the present investigation, silver nanoparticles synthesized by a soil Bacillus sp. were characterized using UV–vis spectroscopy, FTIR, SEM, and EDS. The antibacterial potential of biosynthesized silver nanoparticles, standard antibiotics, and their conjugates were evaluated against multidrug-resistant biofilm-forming coagulase-negative S. epidermidis strains, S. aureus, Salmonella Typhi, Salmonella Paratyphi, and V. cholerae. Interestingly, silver nanoparticles (AgNPs) showed remarkable antibacterial activity against all the test strains with the highest activity against S. epidermidis strains 145 and 152. In addition, the highest synergistic effect of AgNPs was observed with chloramphenicol against Salmonella typhi. The results of the study clearly indicate the promising biomedical applications of biosynthesized AgNPs.     

Comparative antibacterial potential of silver nanoparticles prepared via chemical and biological synthesis

New and improved approaches are urgently needed to fight the increasing number of multi-drug resistant bacteria. The antibacterial effect of silver nanoparticles (AgNPs) prepared by standardized chemical and biological syntheses is compered here. Biological systems included extracts of Opuntia ficus-indica mucilage and extracellular growth broth of Aspergillus niger and Bacillus megaterium. The nanoparticles were characterized by infrared spectroscopy, IR, and transmission electron microscopy. All of the AgNPs shared characteristic IR peaks and had an average size of 20–60 nm. The AgNPs were mainly spherical regardless of synthetic path. The synthesis based on the extracellular broth of the fungus, due to the highest biomass and active compounds concentration, resulted in a high yield of nanoparticle formation. These AgNPs also exhibited the highest inhibition zone against Salmonella typhimurium and Staphylococcus aureus. The syntheses reported here have no significant influence on AgNPs physical characteristics, as compared to literature, but represent processes with shorter reaction time. Additionally, the fungal based nanoparticles have superior antibacterial characteristics.     

Green Synthesis of Silver Nanoparticles Using the Plant Extract of Acer oblongifolium and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches

In this study, the antibacterial and antifungal properties of silver nanoparticles synthesized with the aqueous plant extract of Acer oblongifolium leaves were defined using a simplistic, environmentally friendly, reliable, and cost-effective method. The aqueous plant extract of Acer oblongifolium, which served as a capping and reducing agent, was used to biosynthesize silver nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to analyze the biosynthesized Acer oblongifolium silver nanoparticles (AgNPs). Gram-positive bacteria (Bacillus paramycoides and Bacillus cereus) and Gram-negative bacteria (E. coli) were used to test the AgNPs’ antibacterial activity. The presence of different functional groups was determined by FTIR. The AgNPs were rod-like in shape. The nanoparticles were more toxic against Escherichia coli than both Bacillus cereus and Bacillus paramycoides. The AgNPs had IC₅₀ values of 6.22 and 9.43 and mg/mL on HeLa and MCF-7, respectively, proving their comparatively strong potency against MCF-7. This confirmed that silver nanoparticles had strong antibacterial activity and antiproliferative ability against MCF-7 and HeLa cell lines. The mathematical modeling revealed that the pure nanoparticle had a high heat-absorbing capacity compared to the mixed nanoparticle. This research demonstrated that the biosynthesized Acer oblongifolium AgNPs could be used as an antioxidant, antibacterial, and anticancer agent in the future.     

Antibacterial effect of silver nanoparticles prepared in bipolymers at moderate temperature

The purpose of this study was to investigate the antibacterial effect of silver nanoparticles in chitosan–poly(ethylene glycol) suspension. The silver nanoparticles (AgNPs) were prepared by use of an environmentally benign method from chitosan (Cts) and poly(ethylene glycol) (PEG) at moderate temperature and with stirring for different times. Silver nitrate (AgNO₃) was used as the metal precursor and Cts and PEG were used as solid support and polymeric stabilizer, respectively. The antibacterial activity of silver–chitosan–poly(ethylene glycol) nanocomposites (Ag–Cts–PEG NCs) against Staphylococcus aureus, Micrococcus luteum, Pseudomonas aeruginosa, and Escherichia coli was tested by use of the Mueller–Hinton agar disk-diffusion method. Formation of AgNPs was determined by UV–visible spectroscopy; surface plasmon absorption maxima were observed at 415–430 nm in the UV–visible spectrum. The peaks in the XRD pattern confirmed that the AgNPs had a face-centered cubic structure; peaks of contaminated crystalline phases were not observed. Transmission electron microscopy (TEM) revealed that the AgNPs synthesized were spherical. The optimum stirring time for synthesis of the smallest particle size (mean diameter 5.50 nm) was 12 h. The AgNPs in Cts–PEG were effective against all the bacteria tested. Higher antibacterial activity was observed for AgNPs with smaller size. These results suggest that AgNPs can be used as an effective inhibitor of bacteria and can be used in medical applications. These results also suggest that AgNPs were successfully synthesized in Cts–PEG suspension at moderate temperature with different stirring times.     

A comparative study of the antibacterial mechanisms of silver ion and silver nanoparticles by Fourier transform infrared spectroscopy

In this study, we performed the first comparative study of the antibacterial mechanisms of silver ion (Ag⁺) and silver nanoparticles (AgNPs) on Escherichia coli (E. coli) using Fourier transform infrared (FTIR) spectroscopy. Through a thorough analysis of the FTIR spectra of E. coli after silver treatment in the spectral regions corresponding to thiol group, protein, lipopolysaccharide (LPS), and DNA, we were able to reveal a multifaceted antibacterial mechanism of silver at the molecular level for both Ag⁺ and AgNPs. Features of such mechanism include: (1) silver complexes with thiol group; (2) silver induces protein misfolding; (3) silver causes loss of LPS from bacterial membrane; (4) silver changes the overall conformation of DNA. Despite the similarities between Ag⁺ and AgNPs with respect to their antibacterial mechanisms, we further revealed that Ag⁺ and AgNPs display quite different kinetics for silver-thiol complexation and loss of LPS, with Ag⁺ displaying fast kinetics and AgNPs displaying slow kinetics. At last, we proposed a hypothesis to interpret the observed different behaviors between Ag⁺ and AgNPs when interacting with E. coli.     

Photodynamic cytotoxic and antibacterial evaluation of Tecoma stans and Narcissus tazetta mediated silver nanoparticles

Nanobiotechnology is the intersection of nanotechnology and biology, where nano systems are applied to help study biological systems. There is a growing interest of researchers in the application of nanotechnology in improving the efficacy of photodynamic therapy. In this study, the antioxidant, photodynamic, anticancer, and antibacterial potential of plant extracts and silver nanoparticles (AgNPs) were investigated. In order to synthesize AgNPs, 10 g of dried powder of Tecoma stans and Narcissus tazetta was boiled in deionized water (100 ml) and mixed with aqueous solution of silver metals, resulting in the formation of AgNPs. The synthesized AgNPs were spherical having size in a range of 15–100 nm. The application of extract (50 µl) and AgNPs to rhabdomyosarcoma cell line showed a decreased cell viability (%). Photodynamic study revealed an improvement in photosensitizer efficacy on introducing AgNPs. Both plant extracts and AgNPs had significant effect against methicillin resistant Staphylococcus aureus (MRSA) as well as sensitive Staphylococcus aureus with minimum inhibitory concentration (MIC) values of AgNPs lower (32–256 µg/ml) than the plant extracts. According to the current findings, these AgNPs have an enhancing effect on the photodynamic cytotoxic potential of plant extracts. Because of biological efficacy, these AgNPs may play a crucial role in determining therapeutic potential of Tecoma stans and Narcissus tazetta.     

Green Synthesis of Silver Nanoparticles Using Water Extract from Galls of <Emphasis Type="Italic">Rhus Chinensis</Emphasis> and Its Antibacterial Activity

The green synthesis of silver nanoparticles (AgNPs) has been proposed as a simple, eco-friendly and cost effective alternative to chemical and physical methods. The Rhus chinensis plant is one of the well studied medicinal plant and its galls find excellent clinical and therapeutic applications. The present study reports the use of water extract from galls of R. chinensis as a reducing agent and formation of AgNPs from silver nitrate solution by a green synthesis route. The AgNPs formation was observed visually by color change and the absorbance peak at 450 nm was observed by UV–Visible spectrophotometer. The shape, size, and morphology of synthesized AgNPs were monitored by transmission electron microscopy and field-emission scanning electron microscopy. The face centered cubic structure of AgNPs was confirmed by X-ray diffraction pattern and element composition by energy dispersive X-ray analysis. The Fourier transform infrared spectroscopy spectrum revealed that the presence of components acts as a reducing and capping agent. The antibacterial activity was performed using the agar well diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were determined by broth dilution and spread plate method respectively. Synthesized nanoparticles were spotted as triangular and hexagonal shape and the particle size was around 150 nm.     

Green Synthesis of Silver Nanoparticles by Extracellular Extracts from Aspergillus japonicus PJ01

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO₃ (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag⁺ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO₃ concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO₃ concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.     

Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity

Biosynthesis of silver nanoparticles (AgNPs) was achieved by a novel, simple green chemistry procedure using citrus sinensis peel extract as a reducing and a capping agent. The effect of temperature on the synthesis of silver nanoparticles was carried out at room temperature (25°C) and 60°C. The successful formation of silver nanoparticles has been confirmed by UV-vis, FTIR, XRD, EDAX, FESEM and TEM analysis and their antibacterial activity against Escherichia coli, Pseudomonas aeruginosa (gram-negative), and Staphylococcus aureus (gram-positive) has been studied. The results suggest that the synthesized AgNPs act as an effective antibacterial agent.     

Antibacterial and antibiofilm activity of nanochelating based silver nanoparticles against several nosocomial pathogens

The emergence of multi‐drug resistant (MDR) bacteria and dynamic pattern of infectious diseases demand to develop alternative and more effective therapeutic strategies. Silver nanoparticles (AgNPs) are among the most widely commercialized engineered nanomaterials, because of their unique properties and increasing use for various applications in nanomedicine. This study for the first time aimed to evaluate the antibacterial and antibiofilm activities of newly synthesized nanochelating based AgNPs against several Gram‐positive and ‐negative nosocomial pathogens. Nanochelating technology was used to design and synthesize the AgNPs. The cytotoxicity was tested in human cell line using the MTT assay. AgNPs minimal inhibitory concentration (MIC) was determined by standard broth microdilution. Antibiofilm activity was assayed by a microtiter‐plate screening method. The two synthesized AgNPs including AgNPs (A) with the size of about 20‐25 nm, and AgNPs (B) with 30‐35 nm were tested against Staphylococcus aureus, Staphylococcus epidermidis, Acinetobacter baumannii, and Pseudomonas aeruginosa. AgNPs exhibited higher antibacterial activity against Gram‐positive strains. AgNPs were found to significantly inhibit the biofilm formation of tested strains in concentration 0.01 to 10 mg/mL. AgNPs (A) showed significant effective antibiofilm activity compared to AgNPs (B). In summary, our results showed the promising antibacterial and antibiofilm activity of our new nanochelating based synthesized AgNPs against several nosocomial pathogens.     

Biosynthesis of silver nanoparticle using flowers of Calotropis gigantea (L.) W.T. Aiton and activity against pathogenic bacteria

Silver nanoparticles (AgNPs) from silver nitrate solution are carried out using the flower extract of Calotropis gigantea. Silver nanoparticles were characterized by UV–vis spectrophotometer, X-Ray diffractometer (XRD). Reduction of silver ions in the aqueous solution of silver during the reaction was observed by UV–vis spectroscopy. Crystalline nature of synthesized silver nanoparticles was studied by XRD pattern, refraction peak using the Scherrer’s equation. Antibacterial activity of the silver nanoparticles was performed by disc diffusion method against Bacillus subtilis, Pseudomonas putida and Escherichia coli. The antibacterial activity of synthesized silver nanoparticles by flower extract of C. gigantea was found against B. subtilis (10 mm). Synthesised AgNPs has the efficient antibacterial activity against Gram positive bacteria.     

Green synthesis of chitosan-stabilized silver-colloidal nanoparticles immobilized on white-silica-gel beads and the antibacterial activities in a simulated-air-filter

This study explored the green synthesis and immobilization of colloidal silver nanoparticles (AgNPs) on a solid compatible support. Its antibacterial properties in reusable air filters are also discussed. The chitosan stabilized colloidal AgNPs (chi-AgNPs) were prepared using visible light irradiation in methanol. The UV–Vis, FTIR spectra, and TEM confirmed the chi-AgNPs formation. The immobilization technique of chi-AgNPs on the surface of white-silica-gel beads, which was previously coated chitosan (chi-SiG), was effective. The immobilized silver particles (AgNPs-[chi-SiG]) were solid, stable, dispersed, and nano-size. Both AgNPs-[chi-SiG] and chi-SiG exhibited antibacterial properties and prevented the growth of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria in agar media. Air filter containing the AgNPs-[chi-SiG] showed high antibacterial activity against Bacillus subtilis in the air.     

Daphne mucronata-mediated phytosynthesis of silver nanoparticles and their novel biological applications,compatibility and toxicity studies

This contribution reports the biosynthesis of silver nanoparticles (AgNPs) using aqueous leaf extracts of D. mucronata and their diverse applications. Synthesized AgNPs were characterized using diverse techniques, i.e. UV, XRD, EDS, SEM, TEM, FTIR and TGA/DTA. These techniques confirmed the authenticity of the synthesized nanoparticles. The bimodulated AgNPs revealed the highest radical scavenging potential, i.e. 86.4% relative to plant extract at 600?μg/ml. Escherichia coli was found to be the most susceptible strain to AgNPs. Growth of vancomycin-resistant Staphylococcus aureus was also inhibited. Hemolytic activity revealed negligible hemolysis, indicating the biocompatible nature of biomodulated AgNPs. Furthermore, no mutagenic properties were shown by the biogenic AgNPs. Synthesized nanoparticles possessed promising insecticidal potential and had no phytotoxic activity. No haemagglutination was observed for biogenic AgNPs.     

Phytogenic-mediated silver nanoparticles using Persicaria hydropiper extracts and its catalytic activity against multidrug resistant bacteria

Multidrug resistance (MDR) is one of the major global threats of this century. So new innovative approaches are needed for the development of existing antibiotics to limit antibacterial resistance. The current study was aimed to utilize extracts of root, stem, and leaves of Persicaria hydropiper for the synthesis of silver nanoparticles (AgNPs) using standard procedure. Synthesis of AgNPs was evident from the change in color of the solution to dark brownish and then it was further revealed by UV–Vis and Fourier Transformed Infrared Spectroscopy (FTIR). UV–Vis spectroscopy has revealed absorbance peak at 370 nm while, FTIR spectrum displayed that aromatics amines were used as reducing agent in the fabrication of AgNPs. In addition, Scanning Electron Microscopy (SEM micrograph) displaying tetrahedron, spherical and oval shapes of synthesized AgNPs whereas, average size of synthesized AgNPs was found in the range of 32–77 nm. Beside this, it was also observed that the potency of antibiotics against MDR bacteria increased after coating with synthesized AgNPs i.e., the potency of Ceftazidime and Ciprofloxacin increased up to 450% and 500% against Bacillus respectively while, the potency of Gentamicin, Vancomycin and Linezolid increased up to 150%, 200% and 58% against Bacillus, Staphylococcus, and Proteus species respectively. Furthermore, it was concluded that utilizing AgNPs in combination with commercially available antibiotics would provide an alternate therapy for the treatment of infectious diseases caused by MDR bacteria.     

In Vitro Antioxidant,Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using Equisetum Species: A Green Approach

The ethanolic extracts of three Equisetum species (E. pratense Ehrh., E. sylvaticum L. and E. telmateia Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. FTIR data revealed the functional groups of biomolecules involved in AgNPs synthesis, such as O-H, C-H, C=O, C-O, and C-C. EDX spectroscopy was used to highlight the presence of silver, while DLS spectroscopy provided information on the mean diameter of AgNPs, that ranged from 74.4 to 314 nm. The negative Zeta potential values (−23.76 for Ep–AgNPs, −29.54 for Es–AgNPs and −20.72 for Et–AgNPs) indicate the stability of the obtained colloidal solution. The study also focused on establishing the photocatalytic activity of AgNPs, which is an important aspect in terms of removing organic dyes from the environment. The best photocatalytic activity was observed for AgNPs obtained from E. telmateia, which degraded malachite green in a proportion of 97.9%. The antioxidant action of the three AgNPs samples was highlighted comparatively through four tests, with the best overall antioxidant capacity being observed for AgNPs obtained using E. sylvaticum. Moreover, the biosynthesized AgNPs showed promising cytotoxic efficacy against cancerous cell line MG63, the AgNPs obtained from E. sylvaticum L. providing the best result, with a LD₅₀ value around 1.5 mg/mL.