Proteins of the seeds ofRicinis communis. I. A study of the water-soluble proteins of the seeds and meal ofRicinis communis

The electrophoretic spectrum of the water-soluble proteins of the seeds and meal of the castor-oil plant has been studied. A correlation has been found between a decrease in the relative amount of electrophoretic components with R_f 0.04 and 0.11 and the hemagglutinating activity of the meal.Institute of the Chemistry of Plant Substances of the Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 630–631, September–October, 1960.     

A study of the water-soluble proteins of the seeds of the cotton plants Gossypium hirsutum and G. barbadense

It has been shown by electrophoresis in polyacrylamide gel that different enzymes with similar electrophoretic mobilities may be localized in one and the same zone of water-soluble cottonseed proteins. Some zones not stained by the usual protein dyes also possess enzymatic activity. It has been established that the majority of the electrophoretic fractions consist of a series of polypeptides with different molecular weights, mainly of low-molecular-weight nature. The results are given of an investigation of the peroxidase, -amylase, lipase, NADH-DCPIP oxidoreductase, and NADPH-DCPIP oxidoreductase activities of the water soluble fraction of the proteins of the seeds of the species mentioned.Institute of Experimental Plant Biology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 488–491, July–August, 1986.     

A study of the water-soluble proteins of the seeds of the cotton plants Gossypium hirsutum and G. barbadense

It has been shown by electrophoresis in polyacrylamide gel that different enzymes with similar electrophoretic mobilities may be localized in one and the same zone of water-soluble cottonseed proteins. Some zones not stained by the usual protein dyes also possess enzymatic activity. It has been established that the majority of the electrophoretic fractions consist of a series of polypeptides with different molecular weights, mainly of low-molecular-weight nature. The results are given of an investigation of the peroxidase, α-amylase, lipase, NADH-DCPIP oxidoreductase, and NADPH-DCPIP oxidoreductase activities of the water soluble fraction of the proteins of the seeds of the species mentioned.     

Enzymatic-chemical isolation of lipids, proteins, and gossypol from cotton seeds and meal. I

An enzymatic-chemical method of isolating the complex of lipids, gossypol pigments, and water-soluble proteins from cotton seeds and meal has been developed which permits: a) the achievement of the maximum yield of all types of lipids without their degradation; b) a rise in the qualitative and food value of the oil and protein products isolated from the seeds, with an increase in the solubility of the latter; c) the extraction from cotton seeds of more than 75% and from cottonseed meal of 42% of the free gossypol. The method, in combination with Folch's procedure, is suitable for the quantiative estimation of the lipids, strongly bound to the protein of the nucleus.Institute of Chemistry of Plant Substances, Uzbek Academy of Sciences, Tashkent. Translated from Khimiya Prirodynykh Soedinenii, No. 5, pp. 621–628, September–October, 1991.     

Enzymatic-chemical isolation of lipids,proteins, and gossypol from cotton seeds and meal. I

An enzymatic-chemical method of isolating the complex of lipids, gossypol pigments, and water-soluble proteins from cotton seeds and meal has been developed which permits: a) the achievement of the maximum yield of all types of lipids without their degradation; b) a rise in the qualitative and food value of the oil and protein products isolated from the seeds, with an increase in the solubility of the latter; c) the extraction from cotton seeds of more than 75% and from cottonseed meal of 42% of the free gossypol. The method, in combination with Folch's procedure, is suitable for the quantiative estimation of the lipids, strongly bound to the protein of the nucleus.     

Proteins of the seeds of Ricinus communis. IV. Amino acid sequence of the alanine subunit of ricin T from the seeds of Central Asian caster oil plant. Peptides from limited tryptic hydrolysis

To establish the primary structure of the CM-alanine subunit of ricin T, its limited tryptic hydrolysis has been performed. With the aid of Aminex Q-150S, followed by high-voltage paper electrophoresis, 14 peptides, amounting in total to 256 amino acid residues, have been isolated. The complete amino acid sequences of nine peptides and the partial sequences of five have been determined.Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 243–248, March–April, 1988.     

A study of the dioxane lignin of Ricinus communis

The dioxane lignin (DLA) isolated from a powder of the stems the castor-oil plant by a modification of Pepper's method has been investigated. The yield of DLA amounted to 19.85% on the Komarov lignin. On the basis of elementary and functional analysis, a developed semiempirical formula has been proposed for it from which it can be seen that the amounts of methoxy, aliphatic hydroxy, and carbonyl groups are high and that of phenolic hydroxyl low. UV, IR, and PMR spectra have been taken, and the molecular weight of the material has been determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 501–503, July–August, 1986.     

A study of the dioxane lignin of Ricinus communis

The dioxane lignin (DLA) isolated from a powder of the stems the castor-oil plant by a modification of Pepper's method has been investigated. The yield of DLA amounted to 19.85% on the Komarov lignin. On the basis of elementary and functional analysis, a developed semiempirical formula has been proposed for it from which it can be seen that the amounts of methoxy, aliphatic hydroxy, and carbonyl groups are high and that of phenolic hydroxyl low. UV, IR, and PMR spectra have been taken, and the molecular weight of the material has been determined.     

Acetone powder from dormant seeds of Ricinus communis L

The influence of several factors on the hydrolytic activity of lipase, present in the acetone powder from dormant castor seeds (Ricinus communis) was evaluated. The enzyme showed a marked specificity for short-chain substrates. The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30 degrees C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. The toxicology analysis of the acetone powder from genotype Nordestina BRS 149 showed a higher ricin (toxic component) content, a lower 2S albumin (allergenic compound) content, and similar allergenic potential compared with untreated seeds.     

Phytochemical investigation characterisation and anticonvulsant activity of Ricinus communis seeds in mice

The ethanol extract of the dried, powdered hull portion of Ricinus communis seeds indicated the presence of alkaloids, steroids, flavonoids, glycosides and phenolics, amongst others. Ricinine was isolated as an active constituent and characterised by various chemical and spectroscopic techniques. The anticonvulsant activity of the isolated compound was evaluated in mice using the maximal electroshock (MES) model. The isolated compound at a dose of 60?mg?kg?1 body weight, orally, significantly (p?相似文献