共查询到17条相似文献,搜索用时 93 毫秒
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在pH=6.9的BR缓冲体系中,灿烂甲酚蓝与肌苷反应,形成离子缔合物,溶液颜色发生改变。其最大褪色波长位于630 nm处,在褪色波长处,肌苷的浓度与溶液的褪色呈良好的线性关系,可用于肌苷的测定。 肌苷的浓度在0.18×10-6~6.0×10-6 g/mL范围内符合比尔定律,相关系数r=0.999 3,摩尔吸光系数为1.13×104 L/(mol·cm),检出限为53.5×10-9 g/mL。 方法简便、准确、灵敏,可用于片剂中肌苷的测定。 相似文献
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研究了肌苷发酵液不同pH值、树脂预平衡条件、饱和树脂的洗菌、淋洗条件对肌苷离子交换提取和解吸工艺影响情况,并对其离子交换吸附机理进行了详细探讨,旨在确定最佳提取工艺条件。 相似文献
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肌苷和肌苷酸测定的新方法研究 总被引:7,自引:0,他引:7
尚四华 《理化检验(化学分册)》2001,37(10):463-464
采用紫外吸光光度法与高压纸电泳法联用,对次黄嘌呤类物质-肌苷及肌苷酸含量测定,试验了高压纸电泳法分离肌苷及肌苷酸的最佳条件,并对肌苷口服液等样品进行分离和测定。 相似文献
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肌苷在玻碳电极上的伏安法研究 总被引:4,自引:0,他引:4
应用线性扫描伏安法(LSV),微分脉冲伏安法(DPV)和循环伏安法(CV)对肌苷在玻碳电极(GCE)上的阳级伏安行为进行了研究,发现在0.1mol/L磷酸氢二纳溶液中,于1.42V(vs.Ag/AgCl)左右产生一个阳极氧化峰,峰电流与肌苷浓度在5×10-4~8×10-2g/L范围内呈线性关系,峰电位随溶液pH值增加而降低。肌苷在与次黄嘌呤、黄嘌呤、尿酸共存时,可得4个分辨良好的阳极氧化峰,它们的峰电位分别为1.42V、1.07V、0.72V和0.26V。用本法不需分离直接测定了药物针剂和加标尿样。实验结果表明肌苷的电极反应为扩散控制不可逆过程。 相似文献
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研究了一种快速、简便测定大鼠心肌组织中次黄嘌呤(HX)、肌苷(I)和腺苷(ADO)的高效液相色谱法。采用WatersμBondapakC_(18)色谱柱,含7%甲醇的2mmol/L磷酸钠缓冲液(pH=6.0)为流动相,等比洗脱,254nm处紫外检测,12min内即可分析一个样品。本法生物样本预处理简单,因而平均回收率达95%以上。HX在5.0~500ng,I和ADO在10~1000ng范围内,线性关系良好,相对标准偏差分别低于3.40%(日内)和5.20%(日间),并测定了30份心肌组织中次黄嘌呤、肌苷和腺苷的含量。对所用的色谱分析条件进行了讨论。 相似文献
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利用模式识别技术识别鱼肉的鲜度 总被引:2,自引:1,他引:2
利用模式识别技术与紫外分光不镀分析法结合,根据鱼肉中的肌苷酸和肌苷的含量对鱼肉鲜度进行了识别,采用人工神经网络法和逐步判别分析法两种模式识别技术,判别结果令人满意。 相似文献
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Inosine,i.e.hypoxanthineriboside,hasgreatsignificanceinmedicinemd..t.y['J.Itisalsowidelyusedinfoodindustrybecauseofitsstrongfunctionof..increasingdelicateflavor.Atpresent,thedomesticinosineproducingtechnologyL'Jmainlyconcentratesontheadsorption--elutionprocessofinosinefermentationsolution(pH=3--4)using001X7canonexchangeresinandactivatedcarbon,inwhichthegrossextractionpercentisabout50%,while93%inJapan['].Therefore,theextractiontechnologyshouldbemadeadetailedresearchtoincreasetheextractionperc… 相似文献
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The adsorption characteristics of inosine from fermentation solution on anion exchange resin under the condition of different pH,resin type are investigated.Besides,the desorption conditions are studied under different temperature.The adsorption and desorption mechanism are described to obtain the optimum technological condition of inosine extraction. 相似文献
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Anna Jeliñska 《Reaction Kinetics and Catalysis Letters》2001,72(1):93-100
The kinetics of hydrolysis of inosine was studied in aqueous solution at 353 K over a pH range of 0.45 - 12.13. The decomposition was followed by HPLC. The pH - profile was accounted for by assuming spontaneous water-catalyzed decomposition of the inosine molecules at various degrees of dissociation. 相似文献
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《Analytical letters》2012,45(14):2499-2513
Abstract A dual channel chemiluminescence FIA system for the rapid measurement of the fish freshness index KI where [IMP], [HxR] and [Hx] are, respectively, the inosine 5′-monophosphate, inosine and hypoxanthine contents of fish meat was developed. The system consisted of a pair of chemiluminescence FIAs (CL-FIA); one for the measurement of [IMP] + [HxR] + [Hx], and the other for [HxR] + [Hx]. IMP, HxR or Hx were measured using a reactor comprising immobilized alkaline phosphatase, purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOD). The calibration curves for IMP, HxR or Hx were linear from 0.06 μM to 100 μM for a sample volume of 20 μl. The time for a single KI measurement was less than 1 min. This fish freshness FIA system is rapid, convenient and applicable for fish freshness measurements in real fish samples. 相似文献
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《Analytical letters》2012,45(16):1255-1264
Abstract The DC polarographic behavior of the Cu(II)-inosine complex was investigated, in aqueous solutions of inosine in Britton Robinson buffer solutions at pH's from 1.0 to 4.0. At low concentrations of free inosine ion, only one polarographic wave was found corresponding to a two-electron reduction. Anisotropy of g/ = 2,039, g// = 2,287 and A// = 172G, A/= OG, was observed in the ESR spectrum of the Cu(II)/inosine complex. When the composition ratio of the complex was obtained by both polarography and ESR, it was found that the ratio Cu(II) to inosine was 1:2. The polarographic wave showed a two electron reduction wave. 相似文献
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肌苷制剂的高效液相色谱法测定 总被引:2,自引:0,他引:2
A high performance liquid chromatographic method was established for the determination of inosine intablet,capsule and injection. Sodium benzoate can also be determined.The mobile phase consists of a mixture ofmethanol-water,30:70(V/V).Metronidazole is used as the internal standard.The analytical column is Nucleosil C_(18),7μ.The average recoveries for inosine and sodium benzoate were 99.37±0.62%and 98.83±0.44%. 相似文献