三波长-分光光度法测定黑沙蒿中的总黄酮

以野樱素为对照品、 AlCl3作显色剂,建立了三波长-分光光度法测定黑沙蒿中主要化学成分黄酮类化合物的方法.该方法可校正混浊背景倾斜度的影响,并能消除重叠吸收曲线的干扰.方法的线性范围为1.12～13.44 μg/mL,相关系数r=0.9999,适用于植物中总黄酮的测定.     

Two new flavonoids from Artemisia argyi with their anticoagulation activities

A new flavone glycoside, eupatilin 7-O-β-d-glucopyranoside (1) and a new flavone, 5,6,2′,4′-tetrahydroxy-7,5′-dimethoxyflavone (2), were isolated from Artemisia argyi. Their structures were unambiguously elucidated by extensive spectroscopic analysis. Both flavonoids were evaluated for in vitro anticoagulation activities. Compound 1 significantly extended thrombin time. Compound 2 had obvious effect in increasing prothrombin time.     

沙蒿籽水溶性胶多糖ASPI-A的结构与免疫活性的初步研究

从沙蒿籽中提取出水溶性胶多糖,经柱色谱分离纯化得到一种中性多糖组分ASPI-A.采用高效凝胶渗透色谱法(HPGPC)测定其为均一性多糖,平均分子量为5.42×104Da.经IR,GC部分酸水解、甲基化分析等方法对该多糖的化学结构进行了表征.结果表明,该多糖由阿拉伯糖、甘露糖、葡萄糖及半乳糖组成,其物质的量的比为1∶2.8∶4.9∶1.9.ASPI-A为多分支结构,以(1→4)-β-Glc构成主链,部分葡萄糖C6存在分支,由甘露糖以-4)Man(1-连接在葡萄糖C6位,Glc(1-和Gal(1-连接在甘露糖C4位构成.免疫活性实验结果表明,ASPI-A在10～50μg·mL-1浓度范围内对ConA诱导的小鼠T淋巴细胞增殖反应具有促进作用.     

Phenylpropanoids from Umbilicus pendulinus

Phytochemical investigation of the leaves of Umbilicus pendulinus afforded in addition to 2-O-caffeoyl malate, isoquercitrin and Z-venusol, the new isomer E-venusol. Special NMR experiments were carried out to elucidate the configuration of the two latter compounds.     

Dimeric Coumarin and Phenylpropanoids from Clausena lenis

A new dimeric coumarin, diseselin B ( 1 ), and three new phenylpropanoids, lenisin A–C ( 2 – 4 ), together with eight known O‐terpenoidal coumarins, were isolated from the aerial part of Clausena lenis. Lenisin A ( 2 ) was also isolated from the aerial part of C. excavata. The structures were elucidated on the basis of 1D‐ and 2D‐NMR experiments (HMQC, HMBC, and ¹H,¹H‐COSY).     

少花斑鸠菊中苯丙素类化合物的结构鉴定

利用DiaionHP-20、ToyopearlHW-40、MCI-GelCHP-20、SephadexLH-20、RP18、硅胶等柱色谱法对海南少花斑鸠菊(Vernonia chunii)中的化学成分进行分离纯化,根据理化性质和光谱方法(1HNMR、13CNMR、DEPT、1H-1HCOSY、HSQC、HMBC、ESI-MS、IR)鉴定化合物的结构。从中分离鉴定了7个苯丙素类化合物:3,2′-二-(3,4-二羟基苯基)-5,3′-二羟基-6,7-骈二氢吡喃-3,4-二氢苯骈吡喃酮(Ⅰ)、1,3-二咖啡酰基奎宁酸(Ⅱ)、1,5-二咖啡酰基奎宁酸(Ⅲ)、1-(3,4-二甲氧基苯基)-2,3-二羟甲基-6,7-二甲氧基-1,2,3,4-四氢萘(Ⅳ)、绿原酸(Ⅴ)、咖啡酸(Ⅵ)、阿魏酸(Ⅶ)。其中Ⅰ为新化合物,另外6个化合物为首次从该植物中分离得到。     

Phenylpropanoids from Medicinal Plants: Distribution, Classification, Structural Analysis, and Biological Activity

The literature and our own research results on phenylpropanoids from medicinal plants were reviewed and systematized. Data were presented for the distribution of phenylpropanoids. Their classification was proposed. The biological structure-activity relationships for compounds found in roseroot (Rhodiola rosea), thorny eleutherococcus (Eleutherococus senticosus), common lilac (Syringa vulgaris), purple echinacea (Echinacea purpurea), medicinal melissa (Melissa officinalis), variegated milk-thistle (Silybum marianum), and others were discussed.     

New terpenoids from Amentotaxus formosana

[structure: see text] Amentoditaxone (1), possessing an unprecedented diterpenoid skeleton, along with two new terpenoids, amentotaxin WC (2) and amentotaxone (3), were established by extensive analysis of spectroscopic data.     

Immunosuppressive terpenoids from Tripterygium wilfordii

Two new terpenes,triptobenzene P（1）and wilforone（2）were isolated from Tripterygium wilfordii,as well as 10 known terpenes.Their structures were elucidated by spectroscopic methods.Compounds 2-4,8,10,and 11 showed significant immunosuppressive activities.     

A Lignoid Glycoside and Dimeric Phenylpropanoids from Daphne oleoides

Three new natural products, a lignoid glycoside 1 and two dimeric phenylpropanoids 2 and 3 , along with two known lignans 4 and 5 , were isolated from the BuOH‐ and CHCl₃‐soluble fractions of the whole plant of Daphne oleoides (Thymelaeaceae). The structures of the new compounds were established by spectroscopic techniques, including 2D NMR, as 4‐(β‐D ‐glucopyranosyloxy)‐9′‐hydroxy‐3,3′,4′‐trimethoxy‐7′,9‐epoxylignan ( 1 ), (1R,2S,5R,6R)‐6‐(3‐ethyl‐4‐hydroxy‐5‐methoxyphenyl)‐2‐(4‐hydroxy‐3,5‐dimethoxyphenyl)‐3,7‐dioxabicyclo[3.3.0]octane ( 2 ) and (1R,2S,5R,6S)‐2,6‐bis(3‐ethyl‐4‐hydroxy‐5‐methoxyphenyl)‐3,7‐dioxabicyclo[3.3.0]octane ( 3 ). The other lignans were identified as (+)‐pinoresinol O‐(β‐D ‐glucopyranoside) ( 4 ) and (+)‐medioresinol ( 5 ).     

Norlignans and Phenylpropanoids from Metasequoia glyptostroboides Hu et Cheng

Five new compounds, i.e., the three new norlignans metasequirins G–I ( 1 – 3 ) and the two new phenylpropanoids 7‐(3‐ethoxy‐5‐methoxyphenyl)propane‐7,8,9‐triol (=1‐(3‐ethoxy‐5‐methoxyphenyl)propane‐1,2,3‐triol; 4 ) and 7‐(3‐hydroxy‐5‐methoxyphenyl)propane‐7,8,9‐triol (=1‐(3‐hydroxy‐5‐methoxy‐phenyl)propane‐1,2,3‐triol; 5 ), were isolated from the branches and stems of Metasequoia glyptostroboides Hu et Cheng . Their structures were elucidated by physical, chemical, and spectroscopic methods, including 1D‐ and 2D‐NMR and HR‐ESI‐MS. The cytotoxicites of the five compounds were tested against A549 and Colo 205 cell lines by the MTT method.     

Antimicrobial terpenoids from Pterocarpus indicus

A mixture of loliolide 1 (> 85%) and paniculatadiol 2 (< 15%) was obtained from the ethyl acetate leaf extract of Pterocarpus indicus by silica gel chromatography, while the air-dried flowers afforded lupeol 3 and phytol esters 4. The structures of 1-4 were determined by NMR spectroscopy. Antimicrobial tests on a mixture of 1 and 2 indicated that it has moderate activity against Candida albicans and low activity against Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger. It was found inactive against Staphylococcus aureus, Bacillus subtilis, and Trichophyton mentagrophytes.     

Chemical Composition and Antioxidant and Antibacterial Potencies of the Artemisia ordosica Aerial Parts Essential Oil during the Vegetative Period

As one of the vital shrubs growing in crusted areas in China, Artemisia ordosica (belonging to the Asteraceae family) is abundant in essential oil, and its aerial part’s essential oil has been reported to have some biological activities during the flowering and fruit set stage, and has been used in folk medicine. However, little is known about the biological activities of its aerial part’s essential oil during the vegetative period. Thus, the purpose of this work was to determine the chemical composition and evaluate the antioxidant and antibacterial potencies of the essential oil extracted from A. ordosica aerial parts during the vegetative stage. Gas chromatography coupled with mass spectrometry (GC-MS) revealed that spathulenol (9.93%) and α-curcumene (9.24%), both sesquiterpenes, were the most abundant of the 74 chemical constituents detected in the essential oil of A. ordosica. The antioxidant activity of the essential oil was found to be relatively moderate against 2,2-diphenylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radical (OH^●) radicals. The essential oil exhibited strong antibacterial activity against Staphylococcus aureus, Salmonella abony and Escherichia coli, with minimum inhibitory concentrations (MICs) of 2.5, 5, and 10 μL/mL, respectively. The results indicate that the essential oil of A. ordosica possesses notable antibacterial properties as well as antioxidant capability and can thus be employed as a natural ingredient which can be used as a substitute for antibiotics in the animal feed industry. However, in vivo toxicological studies are still required to determine the safety level and beneficial outcomes of the A. ordosica essential oil for future utilization.     

Phenylpropanoids from Tanacetum baltistanicum with Nematocidal and Insecticidal Activities

Chemistry of Natural Compounds - One new and seven known secondary metabolites 1–8 were isolated, and the nematocidal and insecticidal activities of major compounds from Tanacetum...     

Three New Phenylpropanoids from Inula nervosa Wall.

Three new phenylpropanoid compounds, named nervolans A–C ( 1 – 3 , resp.), together with two other known phenylpropanoids, coniferyl diangelate and sinapyl diangelate, were isolated from the aerial parts of Inula nervosa Wall . (Asteraceae), a traditional Chinese medicinal plant. The structures of 1 – 3 were elucidated by detailed spectroscopic analyses, including HR‐ESI‐MS data and 2D‐NMR spectroscopy. Compounds 1 – 5 exhibited mild inhibitory effects against NO production in LPS‐stimulated RAW264.7 cells.     

Phenylpropanoids from the stem bark of Jacaranda mimosaefolia

Two new compounds: 2-(3',4'-dihydroxyphenyl) ethyl-3-O-α-L-rhamnopyranosyl-4-O-p-hydroxyphenylacetyl-6-O-caffeoyl-β-D-glucopyranoside and 2-(3',4'-dihydroxyphenyl) ethyl-3-O-α-L-rhamnopyranosyl-4-O-piperidine-3-carboxylic acid-6-O-caffeoyl-β-D-glucopyranoside were isolated from the stem bark of Jacaranda mimosaefolia. In addition, the known compounds lupeol, betulinaldehyde, terminic acid, betulinic acid, maslinic acid, β-sitosterol glucoside and isoacteoside were isolated and identified.     

Isolation and structure elucidation of two new compounds from artemisia Ordosica Krasch

Abstract

Two new compounds, namely arteordoyn A (1) and arteordoyn B (2), together with four known compounds, were isolated from the petroleum ether extract of Artemisia ordosica Krasch. The structures elucidation of 1 and 2 were carried out by 1D-NMR (¹H and ¹³C NMR), 2D-NMR (COSY, HSQC, HMBC and NOESY) and HR-ESI-MS spectral analysis.     

Trypanocidal terpenoids from Laurus nobilis L

Trypanocidal constituents of dried leaves of Laurus nobilis L. (Lauraceae) were examined. Activity-guided fractionation of the methanol extract resulted in the isolation of two guaianolides, dehydrocostus lactone (1) and zaluzanin D (2), and a new p-menthane hydroperoxide, (1R,4S)-1-hydroperoxy-p-menth-2-en-8-ol acetate (3). The minimum lethal concentrations of these compounds against epimastigotes of Trypanosoma cruzi were 6.3, 2.5, and 1.4 microM, respectively.