共查询到20条相似文献,搜索用时 15 毫秒
1.
Agirregabiria M Blanco FJ Berganzo J Fullaondo A Zubiaga AM Mayora K Ruano-López JM 《Electrophoresis》2006,27(18):3627-3634
This work describes the SDS-CGE of proteins carried out in microchannels made of the negative photoresist EPON SU-8. Embedded electrophoretic microchannels have been fabricated with a multilayer technology based on bonding and releasing steps of stacked SU-8 films. This technology allows the monolithic integration of the electrodes in the device. A high wafer fabrication yield and mass production compatibility guarantees low costs and high reliability. A poly(methyl methacrylate) (PMMA) packaging allows an easy setup and replacement of the device for electrophoresis experiments. In addition, the wire-bonding step is avoided. The electrophoretic mobilities of four proteins have been measured in microchannels filled with polyacrylamide. Different pore sizes have been tested obtaining their Ferguson plots. Finally, a separation of two proteins (20 and 36 kDa) has been carried out confirming that this novel device is suitable for protein separation. A resolution of 2.75 is obtained. This is the first time that this SU-8 microfluidic technology has been validated for SDS-CGE of proteins. This technology offers better separation performance than glass channels, at lower costs and with an easy packaging procedure. 相似文献
2.
Mario Castaño-Álvarez Agustín Costa-García María Agirregabiria Jesús Miguel Ruano-López 《Talanta》2009,80(1):24-34
A new SU-8 based microchip capillary electrophoresis (MCE) device has been developed for the first time with integrated electrochemical detection. Embedded electrophoretic microchannels have been fabricated with a multilayer technology based on bonding and releasing steps of stacked SU-8 films. This technology has allowed the monolithic integration in the device of the electrochemical detection system based on platinum electrodes. The fabrication of the chips presented in this work is totally compatible with reel-to-reel techniques, which guarantee a low cost and high reliability production. The influence of relevant experimental variables, such as the separation voltage and detection potential, has been studied on the SU-8 microchip with an attractive analytical performance. Thus, the effective electrical isolation of the end-channel amperometric detector has been also demonstrated. The good performance of the SU-8 device has been proven for separation and detection of the neurotransmitters, dopamine (DA) and epinephrine (EP). High efficiency (30,000-80,000 N/m), excellent precision, good detection limit (450 nM) and resolution (0.90-1.30) has been achieved on the SU-8 microchip. These SU-8 devices have shown a better performance than commercial Topas (thermoplastic olefin polymer of amorphous structure) microchips. The low cost and versatile SU-8 microchip with integrated platinum film electrochemical detector holds great promise for high-volume production of disposable microfluidic analytical devices. 相似文献
3.
Effect of exposure dose on the replication fidelity and profile of very high aspect ratio microchannels in SU-8 总被引:1,自引:0,他引:1
We are interested in using SU-8 dense gratings with very high aspect ratio microchannels as the master mold for fabrication of child molds needed for replication. For such applications, the sidewall taper angle and mask replication fidelity of SU-8 are very important. Increasing the exposure time was experimentally observed to decrease the width of the microchannel and the sidewall angle of SU-8 bars. A new diffraction-refraction-reflection model was also developed. The calculated microchannel width and sidewall angle at high exposure dose agreed well with the experimentally observed values indicating that reflection at the silicon substrate was significant. The larger than calculated actual microchannel width for low exposure dose was shown to be due to leaching of unreacted SU-8 in the developer. Dense gratings of high aspect ratio SU-8 bars separated by high aspect ratio (19.1) microchannels were also demonstrated. 相似文献
4.
Rapid sacrificial layer etching for the fabrication of nanochannels with integrated metal electrodes 总被引:1,自引:0,他引:1
We present a rapid etch method to surface-micromachine nanochannels with integrated noble metal electrodes using a single metal sacrificial layer. The method is based on the galvanic coupling of a chromium sacrificial layer with gold electrodes, which results in a 10-fold increase in etch rate with respect to conventional single metal etching. The etch process is investigated and characterized by optical and electrochemical measurements, leading to a theoretical explanation of the observed etch rate based on mass transport. Using this explanation we derive some generic design rules for nanochannel fabrication employing sacrificial metal etching. 相似文献
5.
In order to eliminate the negative effect of erosion processes on electrodes in arc plasma generators, a new scheme of arc discharge was proposed in which the surface of a molten metal acts as electrodes. A plasma reactor was designed on the basis of this concept. The electrophysical characteristics of such a discharge in steam and air as plasma gases were studied. Experiments on destruction of toxic polychlorinated biphenyls and steam coal gasification were performed. 相似文献
6.
Microwell structures were fabricated using SU-8 photoresist for engineering a quasi-three-dimensional (quasi-3D) microenvironment for cultured neuronal cells. SH-SY5Y human neuroblastoma cells were successfully integrated into microwells of a nominal diameter of 100 microm, with or without 10-microm wide microchannels connecting neighboring microwells, in an aspect ratio (ratio of structure depth over width) of approximately 1. With the help of polyethylene glycol stamping and laminin coating, a neuronal-like network was achieved by integrating populations of SH-SY5Y cells with a microwell network pattern. Resting membrane potential establishment was evaluated with confocal microscopy and the potentiometric fluorescent dye tetramethylrhodamine methyl ester. It was found that the intra/extracellular fluorescent intensity ratio (R) was 2.4+/-1.4 [n (number of cells measured)=112] for SH-SY5Y cells on flat SU-8 substrates on day 5 into differentiation, which was not significantly different from the ratio on day 13 into differentiation, 2.0+/-1.8 (n=104) (P>0.05). For cells in the microwell network structures, R was 4.8+/-4.7 (n=51) and 3.9+/-3.2 (n=62) on days 5 and 13 into differentiation, respectively (P>0.5). Cells within the network structures had higher R ratios than on flat substrates, for either day 5 or 13 into differentiation (P<0.01). These results demonstrated that the well network structures, or topographically patterned substrates, were more suitable formats for promoting SH-SY5Y cell resting membrane potential establishment than flat substrates, suggesting the potential to control cellular function through substrate topography engineering. 相似文献
7.
Blagoi G Keller S Persson F Boisen A Jakobsen MH 《Langmuir : the ACS journal of surfaces and colloids》2008,24(18):9929-9932
Bioactive protein patterns and microarrays achieved by selective localization of biomolecules find various applications in biosensors, bio-microelectromechanical systems (bio-MEMS), and in basic protein studies. In this paper we describe simple photochemical methods to fabricate two-dimensional patterns on a Novolac A derivative polymer (SU-8) and, subsequently, their functionalization with biomolecules. Anthraquinone (AQ) derivatives are used to chemically modify and pattern SU-8 surfaces. Features as small as 20 mum are obtained when using uncollimated light. The X-Y spatial resolution of micropatterned AQ molecules is improved to 1.5 mum when a collimated light source is used. This micropatterning process will be important for the functionalization of MEMS-based biosensors. The method saves several processing steps and can be integrated in cleanroom fabrication thus avoiding contamination of the sensor surfaces. 相似文献
8.
HAN Yun-Yu ZHOU Wu LU Shao-Fen ZHOU Rui-LingDepartment of Chemistry Shandong Teachers University Jinan Shandong S China 《中国化学》1996,14(2):147-151
A photosensitive electrode was prepared by electrodepositing a membrane of chlorophyll a (Chla) on a SnO2 optical transparent electrode,with which and a metal counter electrode a Chla photoelectrochemical cell was formed.Photoinduced current (Ii) and photoinduced voltage (Vi) of the cell were measured.The dependence of Ii on the properties of metal electrodes was obvious,which was illustrated with mechanism of Chla photoelectrical effects Ii in this work was as high as 2×10-5 A·cm-2. 相似文献
9.
Since its introduction in the nineties, the negative resist SU-8 has been increasingly used in micro- and nanotechnologies. SU-8 has made the fabrication of high-aspect ratio structures accessible to labs with no high-end facilities such as X-ray lithography systems or deep reactive ion etching systems. These low-cost techniques have been applied not only in the fabrication of metallic parts or molds, but also in numerous other micromachining processes. Its ease of use has made SU-8 to be used in many applications, even when high-aspect ratios are not required. Beyond these pattern transfer applications, SU-8 has been used directly as a structural material for microelectromechanical systems and microfluidics due to its properties such as its excellent chemical resistance or the low Young modulus. In contrast to conventional resists, which are used temporally, SU-8 has been used as a permanent building material to fabricate microcomponents such as cantilevers, membranes, and microchannels. SU-8-based techniques have led to new low-temperature processes suitable for the fabrication of a wide range of objects, from the single component to the complete lab-on-chip. First, this article aims to review the different techniques and provides guidelines to the use of SU-8 as a structural material. Second, practical examples from our respective labs are presented. 相似文献
10.
Tao SL Popat KC Norman JJ Desai TA 《Langmuir : the ACS journal of surfaces and colloids》2008,24(6):2631-2636
SU-8 is a chemically amplified, epoxy-based negative photoresist typically used for producing ultrathick resist layers during device manufacturing in the semiconductor industry. As a simple resist, SU-8 has garnered attention as a possible material for a variety of biomedical applications, including tissue engineering, drug delivery, as well as cell-based screening and sensing. However, as a hydrophobic material, the use of SU-8 is limited due to a high degree of nonspecific adsorption of biomolecules, as well as limited cell attachment. In this work, surface chemistry is utilized to modify the SU-8 surface by covalently attaching poly(ethylene glycol) (PEG) to increase biofunctionality and improve its nonfouling properties. Different molecular weights and concentrations of PEG were used to form films of various grafting densities on SU-8 surfaces. X-ray photoelectron spectroscopy (XPS) was used to verify the presence of PEG moieties on the SU-8 surface. High-resolution C1s spectra show that, with an increase in concentration and immobilization time, the grafting density of PEG also increases. Further, a standard overlayer model was used to calculate the thickness of the PEG films formed. The effect of PEG-modified SU-8 was examined in terms of protein adsorption on the surface and fibroblast-surface interactions. 相似文献
11.
12.
Polyimide and SU-8 microfluidic devices manufactured by heat-depolymerizable sacrificial material technique 总被引:1,自引:0,他引:1
The following paper describes a sacrificial layer method for the manufacturing of microfluidic devices in polyimide and SU-8. The technique uses heat-depolymerizable polycarbonates embedded in polyimide or SU-8 for the generation of microchannels and sealed cavities. The volatile decomposition products originating from thermolysis of the sacrificial material escape out of the embedding material by diffusion through the cover layer. The fabrication process was studied experimentally and theoretically with a focus on the decomposition of the sacrificial materials and their diffusion through the polyimide or SU-8 cover layer. It is demonstrated that the sacrificial material removal process is independent of the actual channel geometry and advances linearly with time unlike conventional sacrificial layer techniques. The fabrication method provides a versatile and fast technique for the manufacturing of microfluidic devices for applications in the field of microTAS and Lab-on-a-Chip. 相似文献
13.
Microchannel devices were constructed from low-temperature co-fired ceramic (LTCC) materials with screen-printed gold (SPG)
electrodes in three dimensions—on all four walls—for self-contained enzyme-linked immunosorbant assays with electrochemical
detection. The microchannel confines the solution to a small volume, allowing concentration of electroactive enzymatically
generated product and nearby electrodes provide high-speed and high-sensitivity detection: it also facilitates future integration
with microfluidics. LTCC materials allow easy construction of three-dimensional structures compared with more traditional
materials such as glass and polymer materials. Parallel processing of LTCC layers is more amenable to mass production and
fast prototyping, compared with sequential processing for integrating multiple features into a single device. LTCC and SPG
have not been reported previously as the basis for microchannel immunoassays, nor with integrated, individually addressable
electrodes in three dimensions. A demonstration assay for mouse IgG at 5.0 ng/mL (3.3 × 10-11 M) with electrochemical detection was achieved within a 1.8 cm long × 290 μm high × 130 μm wide microchannel (approximately
680 nL). Two of four SPG electrodes span the top and bottom walls and serve as the auxiliary electrode and the assay site,
respectively. The other two (0.7 cm long × 97 μm wide) are centered lengthwise on the sidewalls of the channel. One serves
as the working and the other as the pseudoreference electrode. The immunoassay components were immobilized at the bottom SPG
region. Enzymatically generated p-aminophenol was detected at the internal working electrode within 15 s of introducing the enzyme substrate p-aminophenyl phosphate. A series of buffer rinses avoided nonspecific adsorption and false-positive signals. 相似文献
14.
将金(Ⅰ)通过化学沉积法于4℃经9h使之沉积于聚碳酸酯滤膜(孔径100nm)的内孔壁上,从而制得金纳米通道膜。经清洗并干燥后的膜在十八烷基硫醇[CH3(CH2)16CH2SH](0.1+99.9)溶液中浸泡12h,从而使金纳米通道膜被十八烷基硫醇修饰(将此膜简写作C18SH-Mem)并使其呈疏水性。试验在分离装置的样品池中加入阿特拉津和百草枯两种农药的混合溶液,并使其通过C18H37SH-Mem,经过一定时间后,在膜另一端的检测池中对上述两农药分别在222nm及257nm波长处进行检测。结果表明:在检测池中只测得疏水性的阿特拉津而未能测得百草枯,说明亲水性的百草枯不能在疏水性的经修饰的金纳米通道中迁移。据此,应用修饰后的金纳米通道可达到上述两农药的完全分离。 相似文献
15.
The stoichiometry and stability constants of 8-mercaptoquinoline and alkyl-8-mercaptoquinoline complexes of Zn(II), Cd(II), Pb(II), Ni(II), Bi(III) and Ag(I) were determined potentiometrically in dimethylformamide. The stability of the 8-mercaptoquinolinates decreases in the order Ag(I) Bi(III) Ni(II) Pb(II) Cd(II) Zn(II). Metal 7-methyl-8-mercaptoquinolinates are the most stable. The presence of the alkyl group in the 2-position (which has a steric effect) lowers the strength of metal-ligand bonding. 相似文献
16.
We present a new approach for contactless conductivity detection for microchip-based capillary electrophoresis (CE). The detector integrates easily with well-known microfabrication techniques for glass-based microfluidic devices. Platinum electrodes are structured in recesses in-plane with the microchannel network after glass etching, which allows precise positioning and batch fabrication of the electrodes. A thin glass wall of 10-15 microm separates the electrodes and the buffer electrolyte in the separation channel to achieve the electrical insulation necessary for contactless operation. The effective separation length is 34 mm, with a channel width of 50 microm and depth of 12 microm. Microchip CE devices with conductivity detection were characterized in terms of sensitivity and linearity of response, and were tested using samples containing up to three small cations. The limit of detection for K+ (18 microM) is good, though an order of magnitude higher than for comparable capillary-based systems and one recently reported example of contactless conductivity on chip. However, an integrated field-amplified stacking step could be employed prior to CE to preconcentrate the sample ions by a factor of four. 相似文献
17.
Wang Y Bachman M Sims CE Li GP Allbritton NL 《Langmuir : the ACS journal of surfaces and colloids》2006,22(6):2719-2725
SU-8 has gained widespread acceptance as a negative photoresist. It is also finding increasing use as a structural material in microanalytical devices. Consequently, methods to tailor the surface properties of SU-8 as well as to micropattern coatings on the surface of SU-8 are needed. The SU-8 photoresist consists of EPON SU-8 resin mixed with the photoacid generator triarylsulfonium hexafluoroantimonate. This photoacid generator can also serve as a photoinitiator generating free radicals when illuminated with UV light. Under the appropriate conditions, sufficient triarylsulfonium hexafluoroantimonate remains within cured SU-8 to act as a source of free radicals and initiate UV-mediated grafting of polymers onto the surface of the SU-8. UV-mediated grafting was used to coat SU-8 surfaces with poly(acrylic acid) and other water-soluble monomers. The SU-8 surface was chemically micropatterned by placing a mask between the UV light and SU-8. The X-Y spatial resolution of micropatterned poly(acrylic acid) on the SU-8 surface was 2 mum. Three applications of these chemically modified SU-8 surfaces were demonstrated. In the first, poly(ethylene glycol) was used to protect the SU-8 from interactions with proteins, yielding a surface resistant to biofouling. In the second demonstration, the SU-8 surface was micropatterned with a cell-resistant layer to guide cellular attachment and growth. In the final application, SU-8 micropallets were encoded with polymer lines. The bar codes were read by either absorbance or fluorescence measurements. Thus, UV-mediated graft polymerization is an efficient and effective method to micropattern coatings onto the surface of SU-8. 相似文献
18.
Fabrication and characterization of poly(methyl methacrylate) microchannels by in situ polymerization with a novel metal template 总被引:2,自引:0,他引:2
A stainless steel template for the fabrication of plastic microfluidic devices has been developed by photolithography and chemical etching technique. The preparation process of the template is simple, rapid, and low-cost. The cross sectional profiles of raised microchannels on the template are trapezoidal. The surface roughness of the templates was controlled down to 190 nm. The template can be used repeatedly to generate devices reproducibly. The microfluidic devices of poly(methyl methacrylate) (PMMA) were fabricated by in situ polymerization using the templates. The reproducibility of the fabricated microchannel is high and the relative standard deviation is 0.7% by the in situ polymerization approach. Some physical properties of the polymerized microchannels were characterized including the transparency, the thermal deformation temperature, and the dimensional information. Current monitoring was used to evaluate the electroosmotic flow within the microchannels under the electric field strength of 300 V/cm. 相似文献
19.
将导电导热性石墨烯(GR)引入光刻胶SU-8中, 制备了具有导电性的复合光刻胶. 采用超景深显微镜和万用表表征了石墨烯在复合光刻胶中的分散性及复合光刻胶的导电性. 通过光刻法将设计的图案转移到氧化铟锡(ITO)玻璃表面制备了一种新型的GR/SU-8图案化电极元件. 进一步在GR/SU-8/ITO表面电化学原位还原CuNPs, 制备了一种新型无酶传感器. 实验结果表明, 该传感器具有优异的电子转移性能, 在110 mmol/L浓度范围内对过氧化氢具有良好的响应(R2=0.999), 同时稳定性优异, 15 d后电流响应仍可保持90%以上, 表明该导电光刻胶可用于电化学传感领域. 相似文献
20.
Outstanding issues regarding the film formation, redox switching characteristics and the oxygen evolution reaction (OER) electrocatalytic behaviour of multicycled iron oxyhydroxide films in aqueous alkaline solution have been revisited. The oxide is grown using a repetitive potential multicycling technique, and the mechanism of the latter hydrous oxide formation process has been discussed. A duplex layer model of the oxide/solution interphase region is proposed. The acid/base behaviour of the hydrous oxide and the microdispersed nature of the latter material has been emphasised. The hydrous oxide is considered as a porous assembly of interlinked octahedrally coordinated anionic metal oxyhydroxide surfaquo complexes which form an open network structure. The latter contains considerable quantities of water molecules which facilitate hydroxide ion discharge at the metal site during active oxygen evolution, and also charge compensating cations. The dynamics of redox switching has been quantified via analysis of the cyclic voltammetry response as a function of potential sweep rate using the Laviron-Aoki electron hopping diffusion model by analogy with redox polymer modified electrodes. Steady state Tafel plot analysis has been used to elucidate the kinetics and mechanism of oxygen evolution. Tafel slope values of ca. 60 mV dec(-1) and ca. 120 mV dec(-1) are found at low and high overpotentials respectively, whereas the reaction order with respect to hydroxide ion activity changes from ca. 3/2 to ca. 1 as the potential is increased. These observations are rationalised in terms of a kinetic scheme involving Temkin adsorption and the rate determining formation of a physisorbed hydrogen peroxide intermediate on the oxide surface. The dual Tafel slope behaviour is ascribed to the potential dependence of the surface coverage of adsorbed intermediates. 相似文献