Densitometric TLC Analysis of Azaarenes in Grilled Meat

JPC – Journal of Planar Chromatography – Modern TLC - A TLC method with densitometric detection is described for qualitative and quantitative analysis of seven azaarenes —...     

由SRN1反应生成的三种取代苯并咪唑的薄层定量研究

研究了光诱导自由基链式亲核取代（ＳＲＮＩ）反应生成Ｎ－（α－萘基）苯并咪唑，Ｎ－（β－萘基）苯并咪唑和Ｎ－（α－吡啶基）苯并咪唑的薄层光密度定量方法，并成功地应用于其反应机理的研究，该法简便，快速，准确，线性范围０～３．０μｇ平均回收率９９．８％～１００．５％相对标准偏差〈４．７％。     

Simultaneous Densitometric TLC Analysis of Olmesartan Medoxomil and Hydrochlorothiazide in the Tablet Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - A simple, rapid, and selective densitometric thin-layer chromatographic (TLC) method has been established and validated for...     

Evaluation of a TLC Densitometric Method for Analysis of Azole Antifungal Agents

A new method for identification and quantitative determination of the azole antifungal agents; ketoconazole, bifonazole, fluconazole and itraconazole is described using TLC with densitometric detection. Validation of the method was carried out to confirm its precision (%RSD ranged between 1.31 and 3.45), recovery (99.7–102.4%) and linearity (r  = 0.99287–0.99722) within the concentration range under investigation. The experimental conditions obtained enable the method to be used for both qualitative and quantitative pharmaceutical analysis.     

Evaluation of a TLC Densitometric Method for Analysis of Azole Antifungal Agents

A new method for identification and quantitative determination of the azole antifungal agents; ketoconazole, bifonazole, fluconazole and itraconazole is described using TLC with densitometric detection. Validation of the method was carried out to confirm its precision (%RSD ranged between 1.31 and 3.45), recovery (99.7–102.4%) and linearity (r  = 0.99287–0.99722) within the concentration range under investigation. The experimental conditions obtained enable the method to be used for both qualitative and quantitative pharmaceutical analysis.

     

Microcomputer-Assisted Acquisition and Reduction Of TLC Scanning Densitometric Data

Abstract

The rapid microcomputer processing of quantitative TLC scanning data is described. The microcomputer, using BASIC language, is based on the Intel 8080A microprocessor IC and has 64K bytes of RAM. Data is acquired via a 16-bit integrating ADC. A TLC plate with up to 18 samples may be analyzed in as little as 5 minutes. Graphics capabilities include automatic analysis and depiction of peak heights and peak areas. Display of normal or log-normal plots of data for reference compounds and samples with least-squares linear regression is available. Graphics are directly accessible to permit independent plotting of data.     

Densitometric and Videodensitometric TLC Determination of Timolol and Betaxolol in Ophthalmic Solutions

JPC – Journal of Planar Chromatography – Modern TLC - A new, accurate, rapid TLC method has been established for determination of timolol maleate (Timohexal 0.1%, Timoptic 0.25%) and...     

Determination of Fluvoxamine and Moclobemide in Tablets by Densitometric and Videodensitometric TLC

JPC – Journal of Planar Chromatography – Modern TLC - New, simple, rapid, and accurate thin-layer chromatographic methods have been established for determination of fluvoxamine and...     

Simultaneous Densitometric TLC Analysis of Atorvastatin Calcium and Fenofibrate in the Bulk Drug and in Pharmaceutical Formulations

JPC – Journal of Planar Chromatography – Modern TLC - The combination of atorvastatin calcium and fenofibrate is used for treatment of atherosclerotic vascular diseases. A simple,...     

A Rapid Densitometric TLC Method for Simultaneous Analysis of Costunolide and Dehydrocostus Lactone in Saussurea Costus

Costunolide and dehydrocostus lactone are two guianolide sesquiterpene lactones present in abundance in Saussurea costus, a plant popularly known as `costus' and renowned throughout the world for its medicinal properties. A simple densitometric TLC method for simultaneous quantification of costunolide and dehydrocostus lactone, without derivatization, has been developed as an alternative to gas chromatography. The method enables rapid, sensitive, and reproducible simultaneous analysis of a variety of samples. The method was validated for precision, repeatability, and accuracy in accordance with ICH guidelines. Amounts of costunolide and dehydrocostus lactone in different samples of costus, estimated by use of this method, were in the range 0.46 to 1.00% and 0.81 to 1.21%, respectively. The proposed method is simple, precise, specific, sensitive, and accurate and can be used by the herbal drug industry for routine quality control of the crude drug costus.     

Determination of Fluoxetine and Paroxetine in Pharmaceutical Formulations by Densitometric and Videodensitometric TLC

JPC – Journal of Planar Chromatography – Modern TLC - Two new, simple, rapid, and accurate thin-layer chromatographic methods have been developed for determination of fluoxetine in...     

Determination of Bezafibrate and Ciprofibrate in Pharmaceutical Formulations by Densitometric and Videodensitometric TLC

JPC – Journal of Planar Chromatography – Modern TLC - New simple TLC methods with densitometry and videoscanning have been developed for the quantitation of bezafibrate in Bezamidin...     

Multiple Gradient TLC and Densitometric Analysis of p-Coumaric Acid in Some Medicinal Plants from the Family Solanaceae

JPC – Journal of Planar Chromatography – Modern TLC -     

TLC Densitometric Method for the Quantification of Eugenol and Gallic Acid in Clove

Eugenol and gallic acid are reported from the flower buds of Syzygium aromaticum (L.) Merr. & Perry (clove). Both the compounds have been shown to give interesting biological activities and hence serve as biomarkers. We report a simple TLC densitometric method for the quantification of eugenol and gallic acid in clove. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSD of 0.61 and 1.3 (intraday) and 0.96 and 0.24 (interday) for different concentrations of eugenol and gallic acid respectively. Instrumental precision was 0.24 and 0.21 (% CV) for eugenol and gallic acid respectively. Accuracy of the method was checked by conducting recovery study at two different levels for eugenol and gallic acid and the average percentage recoveries were found to be 99.79% and 97.90% respectively. The contents of eugenol and gallic acid in different samples of clove, as estimated by the proposed method, were found to be in the range of 12.9–14.6% and 0.31–0.61% respectively. The proposed HPTLC method for the estimation gallic acid and eugenol was found to be simple, precise, specific, sensitive and accurate and can be used for routine quality control of clove.     

Simple Densitometric TLC Analysis of Plaunotol for Screening of High-Plaunotol-Containing Plants of Croton stellatopilosus Ohba

JPC – Journal of Planar Chromatography – Modern TLC - Plaunotol, an anti-peptic diterpenoid present in the leaves of Croton stellatopilosus Ohba, was separated from other components of...     

QuEChERS/UPLC-Q-OrbitrapHRMS法测定水产及水产加工品中16种苯并咪唑类与13种硝基咪唑类药物残留

建立了Qu ECh ERS/超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-Q-OrbitrapHRMS)同时检测水产及水产加工品中16种苯并咪唑类和13种硝基咪唑类药物残留的分析方法。样品经乙酸乙酯提取,浓缩近干,用30%(体积分数)甲醇-水溶液定容,乙腈饱和正己烷、氨基(NH2)吸附剂和Mg SO4净化。采用Thermo fisher Accucore RP-MS色谱柱(100 mm×2.1 mm,2.6μm),以0.1%(体积分数)甲酸-乙腈为流动相进行梯度洗脱,使用可加热电喷雾离子源(HESI),全扫描/数据依赖二级扫描(Full MS/dd-MS2,Top N=1)模式检测,外标法定量。结果表明,29种药物在0.5~400μg/L范围内线性关系良好(r2≥0.9967),在5种水产及水产加工品基质中进行3个不同水平的加标实验,平均回收率为60.2%~123.3%,相对标准偏差(n=6)为0.1%~21.7%,方法的定量下限为0.5~5.0μg/kg。该法操作简便、精密度高、准确性好,适用于批量水产及水产加工品中29种药物的快速检测。     

Determination of 7-Ketocholesterol and 7-Hydroxycholesterol in Meat Samples by TLC with Densitometric Detection

JPC – Journal of Planar Chromatography – Modern TLC - Concentrations of two oxycholesterols: 7-ketocholesterol and 7- hydroxycholesterol in meat samples (raw and cooked) were determined...     

Normal-Phase TLC Separation of Some Antiarrhythmics. Densitometric Determination of Mexiletine Hydrochloride in Capsules

JPC – Journal of Planar Chromatography – Modern TLC - A normal-phase (NP) TLC method has been established for separation of the five antiarrhythmics - disopyramide, flecainide,...     

Identification and Quantification of Triterpenoid Centelloids in Centella asiatica (L.) Urban by Densitometric TLC

JPC – Journal of Planar Chromatography – Modern TLC - A simple and rapid method for analysis of four major triterpenoids in Centella asiatica preparations is described. Thin-layer...     

Novel TLC Densitometric Method for Quantification Of Solasodine in Various Solanum Species, Market Samples and Formulations

This paper describes a novel TLC densitometric method for the determination of solasodine in various Solanum species (Family Solanaceae). Solasodine does not give absorption in UV range. Hence, ion pair complex of solasodine and acid dye was spotted on TLC plates. A developing solvent with an organic acid ensured in situ color development of the complex which could be successfully quantified at 461 nm. Linearity was found to be in the range of 79.2–495 ng spot⁻¹ with correlation coefficient of 0.995. This method is reproducible, specific, eliminates post derivatization steps and the problem of background interference. The method was completely validated and applied to determine solasodine content in various herb samples, herb extract and their formulations. Accuracy of the method was found to be 98.54 ± 2.8%. No matrix interference was observed.