复合分子印迹聚合物体系选择性富集蛋白质样品中的溶菌酶

考察了功能单体与模板蛋白的反应摩尔比、溶液pH值及离子强度对功能单体与模板蛋白之间相互作用的影响, 得出制备分子印迹聚合物的最佳条件. 在最佳条件下, 以溶菌酶(Lyz)为模板分子, 丙烯酰胺(AA)和N,N’-亚甲基双丙烯酰胺(BisAA)为聚合基质, 二氧化硅为固体制孔剂, 制备了复合分子印迹聚丙烯酰胺凝胶, 并用平衡吸附实验研究了其吸附性能和识别选择性. 研究结果表明, 该聚合物对模板蛋白有较高的亲和性、选择性和吸附容量,可以从蛋白质混合溶液中分离富集模板分子.     

在硅胶表面制备牛血红蛋白分子印迹聚合物

采用表面印迹法, 以乙烯基三甲氧基硅烷修饰的硅胶为载体, 丙烯酰胺为功能单体, N,N-亚甲基双丙烯酰胺为交联剂, 并将改性聚乙烯醇(PVA)作为辅助识别聚合物链(ARPCs)引入聚合体系中, 制备了牛血红蛋白分子印迹聚合物(MIP). 实验使用红外光谱分析了改性PVA的结构特征, 用扫描电镜(SEM)观察MIP的表面形貌, 考察了ARPCs的含量对MIP吸附性能的影响. 吸附动力学实验研究表明, 聚合体系中ARPCs的引入使MIP对模板牛血红蛋白(BHb)的吸附量明显提高|十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)结果显示, MIP对BHb的特异性吸附能力明显提高.     

内分泌干扰物雌酮分子印迹新型吸附功能材料的制备及其选择性能研究料

采用分子印迹本体聚合法,制备了对内分泌干扰物雌酮具有高选择识别能力的分子印迹聚合物。吸附动力学和选择性实验结果表明,与非印迹聚合物相比,印迹聚合物具有较高的吸附容量和吸附速率,对模板分子具有较高的选择性。聚合反应条件对印迹聚合物的吸附和识别性能有重要影响,以丙烯酰胺为功能单体,模板分子、功能单体和交联剂摩尔比为1：3：6,制备的印迹聚合物具有较高的选择和吸附性能。     

谷胱甘肽磁性分子印迹聚合物的制备及吸附性能研究

采用表面分子印迹技术,以谷胱甘肽（GSH）为模板分子,N-乙烯基吡咯烷酮（NVP）和丙烯酰胺（AM）为功能单体,N,N’-亚甲基双丙烯酰胺（MBA）为交联剂,γ-（甲基丙烯酰氧）丙基三甲氧基硅烷（KH570）改性的Fe₃O₄纳米颗粒为磁性载体,制备了对GSH有特异识别性的磁性分子印迹聚合物（GSH-MMIPs）. 利用X射线衍射仪（XRD）、透射电子显微镜（TEM）、红外光谱（FT-IR）和振动样品磁强计（VSM）对聚合物进行了表征,结果表明磁性载体表面成功地包覆了分子印迹聚合物薄层. 静态吸附平衡实验和Scatchard分析结果表明,GSH-MMIPs中存在两类不同的结合位点,平衡解离常数分别为8.786×10^-4 mol/L和5.424×10^-3 mol/L,最大吸附量分别为49.195 mg/g和155.003 mg/g. 与化学组成相同的磁性非印迹聚合物（GSH-MNIPs）相比,GSH-MMIPs对谷胱甘肽有较高的选择吸附性能.     

内分泌干扰物雌酮分子印迹新型吸附功能材料的制备及其选择性能研究

采用分子印迹本体聚合法,制备了对内分泌干扰物雌酮具有高选择识别能力的分子印迹聚合物。吸附动力学和选择性实验结果表明,与非印迹聚合物相比,印迹聚合物具有较高的吸附容量和吸附速率,对模板分子具有较高的选择性。聚合反应条件对印迹聚合物的吸附和识别性能有重要影响,以丙烯酰胺为功能单体,模板分子、功能单体和交联剂摩尔比为1:3:6,制备的印迹聚合物具有较高的选择和吸附性能。     

香豆素分子模板聚合物的合成与性能研究

以香豆素为模板分子, α-甲基丙烯酸(MAA)、丙烯酰胺(MA)、2-乙烯基吡啶(2-VP)和4-乙烯基吡啶(4-VP)为功能单体, 二甲基丙烯酸乙二醇酯(EGDMA)为交联剂, 利用分子模板技术分别在甲苯、甲醇、氯仿和乙腈溶剂中合成了一系列香豆素分子模板聚合物(MTP), 研究了聚合体系组成对模板聚合物吸附特性的影响. 结果表明, 在所合成的模板聚合物中, 以MAA为功能单体, 乙腈为致孔溶剂, 以1∶4∶30的摩尔比加入模板分子、MAA及EGDMA时制备的模板聚合物吸附容量高、印迹效果和选择性好. 此模板聚合物有作为白芷样品中香豆素吸附分离材料的应用前景.     

邻苯二甲酸二甲酯印迹吸附材料的水相合成及固相萃取应用

在硅胶表面以邻苯二甲酸二甲酯(DMP)为模板分子,甲基丙烯酰胺(MAM)为功能单体,N,N-亚甲基双丙烯酰胺(MBAA)为交联剂在甲醇/水体系中制备了DMP印迹聚合物。采用紫外光谱法(UV)研究单体与模板间的相互作用,红外光谱分析印迹聚合物的结构特征。用平衡吸附实验方法研究聚合物对DMP的结合性能,Scatchard法分析聚合物的吸附解离常数(Kd)和最大表观吸附容量(Qmax)。对不同底物的选择性实验表明聚合物对模板分子具有良好的亲和性和选择性。将MIP用作固相萃取(SPE)柱填料,对环境水样中的DMP进行富集试验,回收率为90.28-95.25%,相对标准偏差(RSD)为3.52-7.61%。     

蛋白质印迹模板的制备及在尿液蛋白测定中的应用

以牛血清蛋白(BSA)为分子模板,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDGMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,制备了BSA分子印迹模板,研究了该模板对BSA的吸附性能,并以其作为净化材料去除人尿中的干扰物质,测定了人尿中的蛋白质。结果表明:蛋白质模板吸附和洗脱的最佳pH值分别为5.3和5.6,最佳吸附时间为2h,最大吸附容量为72mg/g。三种人尿样品中蛋白质的含量分别为35.4、18.9、24.2mg/L,回收率为92.4%~103.7%。该方法可用于人尿中蛋白质含量的测定。     

制备牛血红蛋白印迹聚合物的初步研究

采用凝胶法,以丙烯酰胺为单体.N,N'-甲叉双丙烯酰胺为交联剂,制备了牛血红蛋白的分子印迹聚合物.通过考察不同单体用量对所制备的分子印迹聚合物的吸附性能的影响,得到优化制备条件.用吸附溶液的光吸收性质及电镜等对印迹聚合物的选择性吸附特性和形态特征进行表征.结果表明,制备的分子印迹聚合物对模板分子牛血红蛋白可以选择性吸附,具有很好的发展潜力.     

异戊巴比妥分子印迹聚合物的合成和识别性能研究

以异戊巴比妥为模板,分别以丙烯酰胺和甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,用本体聚合法制备了分子印迹聚合物(molecularly imprinted polymer,MIP)。考察了以不同功能单体合成的MIP对模板分子和结构类似物的识别能力和选择性。表明以丙烯酰胺为功能单体合成得到的MIP比甲基丙烯酸为单体的聚合物具有更好的再识别性和选择性,前者对异戊巴比妥的饱和吸附量可达到28．58μmol／g．为理论吸附量的40．9％。     

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation

Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution.     

Synthesis and Adsorption Study of BSA Surface Imprinted Polymer on CdS Quantum Dots

A new bovine serum albumin (BSA) surface imprinting method was developed by the in-corporation of quantum dots (QDs) into molecularly imprinted polymers (MIP), which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical ap-pearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence reso-nance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.     

3-氨基苯硼酸为功能单体在壳聚糖上印迹牛血清白蛋白的研究

以壳聚糖为载体, 3-氨基苯硼酸为功能单体对牛血清白蛋白进行了分子印迹的研究, 并对吸附过程进行Langmuir等温吸附模型的数据处理. 结果表明, 印迹聚合物上形成了对于模板分子有较高的吸附容量和选择性的识别位点, Langmuir等温吸附平衡常数为49.5 mL/mg, 结合位点的最大表观结合量为16.3 mg/g, 证明了该印迹聚合物对于牛血红蛋白和溶菌酶这些非模板蛋白的吸附不具有选择性.     

Preparation and characterization of protein molecularly imprinted polysiloxane using mesoporous calcium silicate as matrix by sol–gel technology

Calcium silicate particles containing mesoporous SiO₂ on the surface (CaSiO₃@SiO₂) were prepared by acid modification of calcium silicate with diluted hydrochloric acid. Bovine serum albumin (BSA) molecularly imprinted polysiloxane (MIP) was synthesized using silane as the functional monomer, BSA as the template and CaSiO₃@SiO₂ particles as the matrix in an aqueous phase. SEM, granulometry, FT-IR and BET analysis were used to characterize the protein MIP. Influence factors on the rebinding capacity of the MIP were investigated, such as the pH in treating CaSiO₃, eluent type and silane proportion. The mass of BSA loading and rebinding on CaSiO₃@SiO₂ and MIP was investigated. The adsorption and recognition properties of the MIP were evaluated and the results showed that the MIP exhibited an obvious improvement in terms of rebinding capacity for BSA as compared with non-imprinted polysiloxane (NIP). BSA imprinted polysiloxane could recognize the template protein by using Lys, Ova, Hb, and Glo as control proteins, and the selectivity factor (β) was above 2.3. The rebinding capacity of BSA imprinted polysiloxane for BSA reached 81.31 mg/g, which was 2.25 times of NIP.     

Novel surface modified molecularly imprinted polymer using acryloyl-β-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution

A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloyl-β-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. Langmuir adsorption model was employed to describe the isotherms, and maximum adsorption capacity was evaluated. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme–cytochrome c, lysozyme–bovine serum albumin, lysozyme–avidin or lysozyme–methylated bovine serum albumin, which showed its high selectivity.     

Molecular imprinting of protein by coordinate interaction

In this article,a novel strong interaction by forming complex between bovine serum albumin(BSA) and copper ion was utilized for the preparation of molecular imprinted hydrogel in aqueous solution.Results show that the inclusion of copper ion in preparation can bridge the template BSA and functional monomers together and improve the imprinting effect compared to the polymer made without copper ion added.High selectivity factor and large adsorption capacity are also observed for the obtained BSA-imprinted ...     

Nonaqueous emulsion polymerization: A practical synthetic route for the production of molecularly imprinted nanospheres

Monodisperse, molecularly imprinted nanospheres were synthesized by nonaqueous (mini)emulsion polymerization using a standard monomer mixture of methacrylic acid and ethylene dimethacrylate containing the drug propranolol as a template. The preparation conditions (solvent, amount of surfactant, and amount of employed template) were extensively varied in order to assess their effect on the properties of the resulting polymer nanoparticles. The molecular recognition capability of the nanospheres was evaluated in batch rebinding experiments, and the effect of the nanosphere preparation conditions as well as of the reaction conditions was investigated. In this way, optimal preparation protocols for molecularly imprinted nanoparticles under nonaqueous conditions with the use of a nonionic emulsifier were identified, which lead to nanospheres with a diameter of around 100 nm having an enhanced capacity of specific template rebinding compared to both nonimprinted nanospheres and to particles obtained by emulsion polymerization in water. Best results were obtained with nanospheres prepared in N,N‐dimethylformamide/n‐hexane with a high functional monomer to template ratio. The enantioselectivity of the rebinding process was also demonstrated. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013     

Surface plasmon resonance sensor chips for the recognition of bovine serum albumin via electropolymerized molecularly imprinted polymers

In this paper,a surface plasmon resonance(SPR)sensor chip for detection of bovine serum album(BSA)was prepared by electropolymerization of 3-aminophenylboronic acid(3-APBA)based on molecularly imprinted polymer(MIP)technique.The surface morphology of MIP and non-imprinted(NIP)flms were characterized by scanning electroscopy(SEM).SEM images exhibited nanoscale cavities formed on the MIP films surface homogeneously due to the removal of BSA templates.The effects of pH,ion strength of rebinding BSA,the specific binding and selective recognition were studied for MIP films.Results indicated that the BSA-imprinted films exhibited a good adsorption of template protein(0.02–0.8 mg/mL)in0.05 mol/L sodium phosphate buffer at pH 5.0 with the limit of detection(LOD)of 0.02 mg/mL.     

The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Alginate/Phosphate Hybrid Microspheres Via the Adjustment of pH Values and Salt Concentration

Summary: Bovine serum albumin imprinted calcium phosphate/alginate hydrogel microspheres were prepared with sodium alginate (SA), (NH₄)₂HPO₄, and using CaCl₂ as gelling agent, bovine serum albumin (BSA) as template in inverse suspension. The optimized rebinding properties of BSA imprinted hydrogel microspheres were investigated by controlling pH value and ionic concentration from the viewpoint of adjusting the process of gelling, removing template and rebinding. The optimized pH values for the imprinting of BSA in gelling, removing template and rebinding process was 4.1, 8.3 and 4.8, respectively. The effect of NaCl concentration on the BSA rebinding was also determined. We provided a strategy to get the optimized imprinting efficiency by altering pH value and ionic concentration in a weakly ionic cross-linked hydrogel system on the process of protein's imprinting.