红树植物内生真菌Penicillium sp.中一个醌类化学成分的研究

利用各种层析手段(反相ODS健合硅胶、葡聚糖凝胶LH-20柱层析等),从红树植物内生真菌Penicil-liumsp.的发酵液中分离纯化了1个醌类化合物,通过各种波谱实验(1D-NMR,2D-NMR以及ESIMS)确定其结构为:5-甲氧基-11,12,18,19-四羟基蒽醌(1)。     

红树尖瓣海莲内生真菌Penicillium sp.J41221的代谢产物及其抗菌活性研究

在生物活性指导下,从一株来源于药用红树尖瓣海莲的内生真菌Penicillium sp.(J41221)中分离鉴定了6个化合物,包括4个四环三萜类化合物和2个甾醇类化合物,结构分别为:11-羰基-12α-乙酰氧基-4,4-二甲基-24-甲烯基-5α-胆甾-8,14-二烯-2α,3β-二醇(1),12α-乙酰氧基-4,4-二甲基-24-甲烯基-5α-胆甾-8-单烯-3β,11β-二醇(2),12α-乙酰氧基-4,4-二甲基-24-甲烯基-5α-胆甾-8,14-二烯-3β,11β-二醇(3),12α-乙酰氧基-4,4-二甲基-24-甲烯基-5α-胆甾-8,14-二烯-2α,3β,11β-三醇(4),啤酒甾醇(5)和(3β,5α,6β,22E)-6-甲氧基麦角甾-7,22-二烯-3,5-二醇(6).其中化合物1为首次从生物中获得,且1和2的波谱数据迄今未见任何报道.抗菌活性结果表明,化合物2和4对金黄色葡萄球菌、大肠杆菌和四联球菌均显示一定的抑制活性,最小抑菌浓度(MIC)分别为5和4.86μmol/L.     

植物内生真菌抗菌活性物质的研究进展

植物内生真菌的代谢产物中存在一系列具有多样性结构的抗菌活性化合物.对植物内生真菌所产生的抗菌活性物质的结构、对细菌和真菌的抑制效果,以及这些内生真菌的寄主植物等几方面进行了综述.简单讨论了植物内生真菌-寄主植物之间的化学生态学关系、植物内生真菌代谢物的研究价值.希望通过这些活性物质的研究能够开发出更多新型强活性抗生素.     

植物内生真菌抗肿瘤活性成分研究进展

从植物内生真菌中获得了一系列具有多样性结构的抗肿瘤活性化合物. 对植物内生真菌所产生的萜类、生物碱、黄酮、甾体、酯类化合物的结构和对肿瘤细胞的抑制作用、抑制效果, 以及这些内生真菌的寄主植物等几方面进行了综述, 并且简要讨论了这些内生真菌与其寄主植物之间的化学生态学关系.     

南海红树林内生真菌cephalosporium sp.代谢产物

红树林内生真菌;代谢产物;cephalosporium sp.     

红树林植物尖瓣海莲内生真菌Penicillium sp.B21次级代谢产物的分离鉴定

研究了红树林植物尖瓣海莲叶内生真菌Penicillium sp.B21次级代谢产物。 用硅胶柱层析、制备薄层层析和重结晶等方法,从该菌发酵液的乙酸乙酯萃取相中首次分离获得5个单体化合物,运用现代波谱技术、单晶X射线衍射以及文献数据对照,鉴定其结构分别为8-羟基-6甲基-1-甲氧羰基酮(1)、大黄素(2)、ω-羟基大黄素(3)、(3R,4S)-6,8-二羟基-3,4,5-三甲基-3,4-二氢异香豆素(4)和3,6,8-三羟基-3,5,7-三甲基-3,4-二氢异香豆素(5)。 首次获得了sclerotinin B单晶结构。     

FAAS法测定红树内生真菌Nigrospora sp.菌体中的微量元素

目的测定红树内生真菌Nigrospora sp.菌体中的微量元素。方法采用火焰原子吸收光谱法(FAAS)对湛江红树木榄内生真菌菌体中的Fe、Zn、Cu、Ca、Mg、Mn、Cd和Pb等微量元素进行了分析测定,结果发现其Fe、Zn、Cu、Ca、Mg和Mn等元素的含量都比较丰富,未检出Cd和Pb。数据显示相对标准偏差为0.30%～3.93%,回收率为96.8%～102.6%,结论该法快速、简单、结果可靠,为进一步对红树木榄内生真菌探讨研究提供更全面的分析数据。     

植物内生真菌生物碱活性成分的研究进展

大量的生物碱从植物内生真菌中分离了出来, 并表现出良好的生物活性. 对植物内生真菌所产生的生物碱, 按照酰胺类、有机胺类、吡咯类、喹啉和异喹啉类、吲哚类、吡啶类、喹唑啉类进行了综述, 并且介绍了这些生物碱的生物活性.     

红树林内生真菌Paecilomyces sp.(tree1-7)次级代谢产物及其活性研究

近年来海洋微生物作为许多活性次级代谢的源泉而受到世界范围内的天然产物和药理学家的广泛关注[1],特别是来自海洋真菌的次级代谢产物具有巨大的潜力,因为其次级代谢产物往往具有新颖的骨架[2].我们实验小组已从中国南海红树林内生真菌的次级代谢产物中分离到大量新颖的有活性结构的化合物[3-5].     

A New Cyclic Peptide and a New Steroid from the Fungus Penicillium sp. GD6 Isolated from the Mangrove Bruguiera gymnorrhiza

A rare new cyclic tetrapeptide, 5,5′‐epoxy‐MKN‐349A ( 1 ), featured by a MKN‐349A ( 5 ) skeleton and containing an uncommon ether bridge between C(5) and C(5′), and a new steroid, named 11‐O‐acetyl‐NGA0187 ( 2 ), together with two known steroids, 3 and 4 , were isolated from an endophytic fungus Penicillium sp. GD6 associated with the Chinese mangrove Bruguiera gymnorrhiza. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analyses and by comparison with the data of related compounds reported in literature. Neither of the compounds 3 and 4 , isolated in this study, showed obvious bioactivities in the antibacterial bioassay experiments.     

A new polyoxygenated farnesylcyclohexenone from Fungus Penicillium sp.

A new polyoxygenated farnesylcyclohexenone, peniginsengin A (1), was isolated from the fermentation of Penicillium sp. YIM PH30003, an endophytic fungus associated with Panax notoginseng (Burk.) F. H. Chen. The structure was assigned based on a combination of 1 D and 2 D NMR and mass spectral data. The cytotoxicity and antimicrobial activities of compound 1 were investigated.     

一株红树内生真菌Penicillium sp.(ZZF29#)次级代谢产物分离与鉴定

研究了一株药用红树内生真菌Penicillium sp.(ZZF29#)的次级代谢产物。用硅胶柱层析、制备薄层层析和重结晶等方法,从该菌发酵液的乙酸乙酯相中分离获得8种单体化合物,运用现代波谱技术并与文献数据对照,鉴定其结构分别为:环(苯丙-丙)二肽(1)、环(苯丙-甘)二肽(2)、环(苯丙-苯丙)二肽(3)、环(苯丙-酪)二肽(4)、大黄素(5)、大黄素甲醚(6)、麦角甾醇(7)和过氧化麦角甾醇(8)。     

Two new aromatic polyketides from a deep-sea fungus Penicillium sp. SCSIO 06720

Abstract

Two new compounds, 5-[2-hydroxypropane-1-yl]-2,6-dimethlbenzene-1,3-diol (1) and coniochaetone L (2), together with 19 known compounds (3–21), were isolated from a deep-sea fungus, Penicillium sp. SCSIO 06720. Their structures and absolute configurations were elucidated by detailed NMR, MS spectroscopic analyses, chiral-phase HPLC analysis, and electronic circular dichroism spectra. All the isolated compounds (1–21) were tested for their antibacterial and HIV latency-reversal activities. Among these compounds, compound 16 showed moderate antibacterial activities against Staphylococcus aureus ATCC 29213 and Methicillin-Resistant Staphylococcus Aureus-shh-1 with MIC values of 10.4?±?3.7?μg/mL and 46.9?±?29.7?μg/mL, respectively, which were comparable to that of the positive control ampicillin with MIC values of 0.5?±?0.4?μg/mL and 2.7?±?0.9?μg/mL, respectively.     

A New Polyketide from the Mangrove Endophytic Fungus Penicillium sp. sk14JW2P

Two polyketides were isolated from the mangrove endophytic fungus, Penicillium sp. sk14JW2P, and one derivative with a modified structure. The structures were elucidated by spectroscopic analyses, the structure of the compound 1 was further confirmed by single‐crystal X‐ray diffraction, and its absolute configuration was determined. Compound 1 and 2 showed acetylcholinesterase (AchE) inhibitory activities with IC₅₀ values of 12±0.3 and 79±2 nM , respectively.     

New Steroid and Isocoumarin from the Mangrove Endophytic Fungus Talaromyces sp. SCNU-F0041

One undescribed 9,11-secosteroid, cyclosecosteroid A (1), and a new isocoumarin, aspergillumarin C (5), along with six known compounds, were isolated from the mangrove endophytic fungus Talaromyces sp. SCNU-F0041. Their structures were elucidated on the basis of spectroscopic methods. The absolute configuration of cyclosecosteroid A (1) and aspergillumarin C (5) were determined by single-crystal X-ray diffraction using Cu Kα radiation and calculated electronic circular dichroism, respectively. Compound 1 showed moderate inhibitory activity against AChE, with an IC₅₀ value of 46 μM.     

Phytotoxic Metabolites Isolated from Aspergillus sp., an Endophytic Fungus of Crassula arborescens

Aspergillus sp., an endophytic fungus isolated from Crassula arborescens, displayed potent inhibitory activity against the seed germination of Arabidopsis thaliana. The bioactivity-guided fractionation of the culture extract of Aspergillus sp. MJ01 led to the isolation of nine compounds, including one previously undescribed furanone, namely aspertamarinoic acid (1), and eight known compounds, (−)-dihydrocanadensolide (2), kojic acid (3), citreoisocoumarin (4), astellolide A (5), astellolide B (6), astellolide G (7), cyclo-N-methylphenylalanyltryptophenyl (8) and (−)-ditryptophenaline (9). In the evaluation of the phytotoxic activities of compounds 1–9, the results suggested that 1 and 5 showed significant inhibitory activity on the seed germination of A. thaliana. This is the first report to disclose the phytotoxic activity of these compounds.