共查询到19条相似文献,搜索用时 62 毫秒
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本文设计并合成了良好水溶性的赖氨酸修饰壳聚糖,并对制备工艺进行了优化.产物通过红外(FTIR)和核磁(1H-NMR)进行了表征,并将其作为壳层材料制备了赖氨酸修饰壳聚糖磁性超微载体.通过光电能谱(XPS)、透射(TEM)、激光粒度仪、X射线衍射(XRD)、磁性能测试(VSM)对载体进行了表征.结果表明,制备的赖氨酸修饰壳聚糖磁性超微载体表面带有大量的氨基(-NH2),粒径分布较为均一(100nm左右),形貌较为规则,并具有良好的超顺磁性,因而该载体具有更加良好的性能. 相似文献
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琼脂微载体的制备及肝细胞附着生长情况的研究 总被引:2,自引:0,他引:2
以琼脂为原料,通过悬浮方法成球制备琼脂微载体,直径100~800μm.利用氨基化修饰琼脂微载体,比较大鼠肝细胞在其上的生长情况,并对培养的肝细胞的形态及葡萄糖、白蛋白的代谢情况进行了测定. 相似文献
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通过微转移模塑法, 在硅片表面制备了圆形点阵、 方形点阵、 沟槽和波浪等4种形貌的壳聚糖(CS)微图形. CS微图形清晰规整, 成型效果良好. 成骨细胞在圆形点阵和方形点阵上借助伪足多攀附于点阵凸起处. 点阵图形对细胞增殖和分化无影响. 在沟槽和波浪微图形中, 成骨细胞大多被限制在沟区, 且沿着沟槽方向伸展. 沟槽和波浪微图形不利于细胞分化, 但能促进细胞增殖. 相似文献
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壳聚糖具有抗菌、抗氧化、增强胶凝特性以及可作为生物活性分子的微型或纳米载体等优点,因此其化学改性和应用近年来受到广泛关注。然而,壳聚糖既不溶于有机溶剂也不溶于水,极大地限制了它的应用。在改性的壳聚糖中,聚乙二醇化壳聚糖不仅能保持壳聚糖的优点,还能提高水溶性,并能有效运输生物活性分子。因此,本文总结了2008-2012年聚乙二醇化壳聚糖作为紫杉醇、阿霉素、5-氟尿嘧啶等小分子载体的最新进展,为今后聚乙二醇化壳聚糖的研究提供有益参考和理论依据。 相似文献
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壳聚糖修饰PLGA阳离子型纳米微球的制备与表征 总被引:6,自引:1,他引:6
采用单乳化-溶剂(O/W)挥发技术制备表面带正电荷的壳聚糖(CHS)修饰聚乙/丙交酯(PLGA)纳米微球(PLGA/CHS), 通过正交试验优化了纳米微球的制备条件. 结果表明, 微球粒径可控制在150~200 nm内, 在pH=4时, 纳米微球表面电位最高为55 mV. 影响微球粒径的主要因素是聚合物的浓度, CHS的分子量和浓度以及介质的pH值对微球表面电位也有明显影响. 制备粒径较小而表面电位较高的PLGA/CHS纳米微球条件为: ρ(CHS)=3 mg/mL, ρ(PLGA)=10 mg/mL, Vo/Va=1/4. SEM图像显示经CHS修饰的PLGA的纳米微球形状规整, 荧光显微观察和XPS分析结果证实CHS包覆于微球表面. 相似文献
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制备了叔胺改性甘草次酸[GA-N(CH3)2]修饰的海藻酸钠[ALG-GA-N(CH3)2], 并在温敏性琼脂糖的辅助作用下, 利用微流体技术获得了高通量、 单分散且粒径可控的ALG-GA-N(CH3)2微凝胶. 考察了Span 80含量、 疏水配体取代度、 样品浓度和水/油相流速对微液滴制备的影响. 研究结果表明, 叔胺基改性可显著改善甘草次酸的亲水性; 在Span 80质量分数为2.0%, 疏水配体取代度小于12%, 样品浓度小于15 mg/mL, 水相流速为1.5 mL/h, 油相流速为6 mL/h条件下, 可获得高通量、 单分散及粒径为200 μm的适用于细胞包封培养的微凝胶球. 同时提供了一种三维培养肝细胞的新方法, 为其在组织工程中的应用奠定了基础. 相似文献
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ZHANG Liguo PAN Jilun** LI Jieliang YU Yaoting The Key Laboratory of Bioactive Materials Nankai University Ministry of Education Tianjin China 《Chinese Journal of Reactive Polymers》2004,(Z1)
1. INTRODUCTION The development of cell based organs and tissue-engineering devices such as hybrid artificial liver requires a large number of cells be cultured for the replacement of damaged tissue [1,2]. Since its introduction in 1967, microcarriers culture has been applied successfully in growing primary cells and cell lines with the advantage of attaining high cell density [3,4]. Spheroid culture is another promising hepatocytes culture method to enhance the cell density and metaboli… 相似文献
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The fructose modified chitosan microcarries (CMs) were prepared by the reaction of glutaraldehyde with fructose-modified chitosan, Various factors that influence the preparation were studied and the reaction conditions were optimized. Morphology of rat hepatocytes cultured on CMs was observed using phase contrast microscope and scanning electron microscope, and the metabolic activities were measured. Rat hepatocytes cultured on CMs retained the spherical shape as they have in vivo and had high metabolic activities. Fructose can enhance the metabolic activity of hepatocytes and the modified CMs are promising scaffold for hepatocytes attachment. 相似文献
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ZHANG Hong YU Yaoting PAN Jilun WANG lianyong Bioactive Materials Research laboratory Nankai University Tianjin 《Chinese Journal of Reactive Polymers》2001,(1)
1. INTRODUCTION Biological artificial liver support systems have been extensively studied in treatment of hepatic failure [1]. In the device, cells included primary hepatocytes [2] and liver cell lines (i.e. C3A, HepG2 [3]) with relatively high differentiations, which are all attachment dependent cells; the cell density is crucially important for its efficiency in clinical application. Microcarrier -attached hepatocytes have been applied in the device in an attempt to raise the cell dens… 相似文献
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Using polyvinyl alcohol(PVA) as raw material and vacuum pump oil as oil phase medium,PVA-based microcarriers were prepared by suspension method,The diameters of the beads were 100-180um,LO2 cells were cultured on PVA-based microcarriers and cytodexⅢ microcarriers.Morphology,attachment and growth rate of LO2 cells were studied. 相似文献
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壳聚糖亲和磁性毫微粒的制备及其对蛋白质的吸附性能研究 总被引:35,自引:0,他引:35
以壳聚糖为包裹材料包埋自制的磁流体 ,制备了具有核 壳结构的磁性毫微粒 ,并偶联色素配基CibacronBlue 3GA(偶联量 1 4 .5μmol/mL)得到了一种新型亲和磁性毫微粒 .结果表明 ,所得亲和磁性微球具有较窄的粒径分布、形状规整 .以牛血清白蛋白 (BSA)和溶菌酶 (Lys)为目标蛋白 ,考察了该亲和磁性毫微粒的吸附性能 ,发现其对BSA和Lys的吸附量分别为 4和 2 8mg/g,吸附行为满足Langmuir吸附等温式 ,且对时间依赖性小而对溶液离子强度敏感 . 相似文献
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以马来酸酐改性的壳聚糖(MAH-chitosan)和丙烯酸(AA)为单体,采用反相微乳液聚合法制备了AA含量分别为77%(AA-Cs)和29%(Cs-AA)壳聚糖/聚丙烯酸复合纳米粒子。TEM结果表明,该复合纳米粒子平均粒径为125~150nm,进而研究了其对牛血红蛋白(Hb)、牛血清白蛋白(BSA)和溶菌酶(Ly)吸附和脱附行为。AA-Cs对各种蛋白的吸附量均较高;而Cs-AA对3种蛋白的吸附则具有一定选择性。AA-Cs对等电点低于脱附pH值的Hb的脱附量较大。Cs-AA粒子在较低pH值(pH=3.4)时对吸附有利,但在高pH值(pH=6.6)进行吸附时,对脱附更为有利。 相似文献
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