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Negative-ion matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectra and tandem mass spectra of flavonoid mono-O-glycosides showed the irregular signals that were 1 and/or 2 Da smaller than the parent deprotonated molecules ([M – H]) and the sugar-unit lost fragment ions ([M – Sugar – H]). The 1 and/or 2 Da mass shifts are generated with the removing of a neutral hydrogen radical (H*), and/or with the homolytic cleavage of the glycosidic bond, such as [M – H* – H], [M – Sugar – H* – H], and [M – Sugar – 2H* – H]. It was revealed that the hydrogen radical removes from the phenolic hydroxy groups on the flavonoids, not from the sugar moiety, because the flavonoid backbones themselves absorb the laser. The glycosyl positions depend on the extent of the hydrogen radical removals and that of the homolytic cleavage of the glycosidic bonds. Flavonoid mono-glycoside isomers were distinguished according to their TOF MS and tandem mass spectra.
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JPC – Journal of Planar Chromatography – Modern TLC -  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC -  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC -  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC -  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC - A sensitive, selective, precise and stability-indicating high-performance thin-layer chromatography (HPTLC) method was developed...  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC - A sensitive, accurate, precise, and stability-indicating high-performance thin-layer chromatographic method has been established...  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC - A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated,...  相似文献   

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JPC – Journal of Planar Chromatography – Modern TLC - A new mode of development for TLC, multidimensional relay development, developed on the basis of half-way and relay development, is...  相似文献   

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