高产花生四烯酸产生菌干菌体中脂肪酸成分的气相色谱-质谱分析

将高产生花生四烯酸（AA）脂肪酸产生菌的干菌体经三氟化硼－甲醇方法甲酯化后,用毛细管气相色谱和质谱进行分析。结果表明干菌体中含有饱和不饱和脂肪酸,其中不饱和脂肪数中以花生四烯酸的含量（70．20％）最高,另外还含有少量的亚油酸和γ-亚麻酸等不饱和脂肪酸,同时给出了各个脂肪酸组分的相对含量。     

被孢霉菌丝体脂肪酸组成的气相色谱-质谱分析

采用气相色谱－质谱对采自云南白马雪山土壤中并经分离和发酵培养的被孢霉菌株SM96的菌丝体油脂中脂肪酸组成进行了定性分析和峰面积相对含量测定，共检测出30种脂肪酸，其中多不饱和脂肪酸含量为62．4％，主要为亚油酸、α－亚麻酸（ALA），还有少量的γ－亚麻酸、二十碳三烯酸、花生四烯酸和二十碳五烯酸（EPA）等，结果表明该菌株能产生EPA。     

用GC-MS法分析柿树叶中的脂肪酸

用乙醚－己烷混合溶剂抽提柿树叶中的脂肪酸,ＮａＯＨ－ＣＨ３ＯＨ交换法甲酯化,毛细管柱ＦＦＡＰ作为分离柱,进行气相色谱－质谱法测定脂肪酸（以甲酯的形式）的组成。共分离出２１种脂肪酸,鉴定出１９种,占总含量的９８．７２％,其中以肉豆蔻酸（６．６８％）、棕榈酸（３０．００％）、硬脂酸（１０．４２％）、十六碳三烯酸（１．４４％）、亚油酸（１．８４％）、十八碳－烯酸（２２．２２％）、亚麻酸（１．２５％）、花     

狭果茶蔗种子脂肪酸的分析

用超临界萃取技术对产于青藏高原上的狭果茶蔗种子中脂肪酸进行萃取，其萃取率为12％。用毛细管气相色谱对萃取的脂肪酸进行了分离和分析。结果表明不饱和脂肪酸的质量分数在90％以上。其中α-亚麻酸(α-Linolenic aeia)为27．4％，γ-亚麻酸(γ-Linoleic aeia)为4．93％，亚油酸(Unoleic aeia)为32．61％。     

桃核中脂肪酸成分分析

：通过GC－MS方法对桃核（核壳，桃仁，种皮）中的脂肪酸化学成分进行分析。鉴定出各种脂肪酸成分及其相对含量，主要成分为棕榈酸，硬酯酸，亚油酸，亚麻酸，油酸等。     

门源茶Biao果实中脂肪酸分析

用超临界萃取技术对青藏高原藏药特有植物门源茶Biao果实中脂肪酸进行萃取，用毛细管气相色谱进行分离和分析，结果表明不饱和脂肪酸在90％以上，其中亚油酸为36.48%，r-亚麻酸为4.5%,α－亚麻酸为29.26%。     

油松籽油脂的提取及其脂肪酸组成与含量的评价

采用索氏提取法提取油松籽中的油脂,得油率为42.9%;对油脂进行甲酯化处理后用气相色谱-质谱联用仪检测其中的脂肪酸组成及含量。实验结果表明,油松籽油中含有7种脂肪酸,分别为肉豆蔻酸10.38%、硬脂酸3.05%、油酸21.98%、亚油酸(13,16-十八碳二烯酸)3.53%、亚油酸(9,12-十八碳二烯酸)38.38%、亚麻酸20.06%和二十碳三烯酸2.62%,其中饱和脂肪酸含量为13%,不饱和脂肪酸含量为87%。     

牡丹籽油中脂肪酸的组成分析

用化学萃取法获得牡丹籽油,然后将样品进行皂化、甲酯化,以毛细管气相色谱-质谱联用法(GC-MS)测定牡丹籽油脂肪酸的组成,共鉴定出14种成分,其中主要成分为亚麻酸和亚油酸,其相对含量分别为57.931%和28.121%。     

门源茶阿（艹/鹿/灬）果实中脂肪酸分析

用超临界萃取技术对青藏高原藏药特有植物门源茶（艹/鹿/灬）果实中脂肪酸进行萃取,用毛细管气相色谱进行分离和分析,结果表明不饱和脂肪酸在90%以上,其中亚油酸为36.48%,r-亚麻酸为4.5%,α-亚麻酸为29.26%.     

低酚棉籽仁中脂肪酸成分的研究

采用气相色谱法测定了９个低酚棉品种中棉籽仁的脂肪酸组成及相对含量。样品所含主要脂肪酸为棕榈酸、油酸、亚油酸。其中亚油酸含量最高，为５８．９０％～６４．８８％。与普通棉籽仁进行对比的结果表明，两者脂肪酸组成一致，但低酚棉的亚油酸与不饱和脂肪酸的平均含量均高于普通棉     

Oil composition and some morphological characters of Crambe orientalis var. orientalis and Crambe tataria var. tataria from Turkey

Native Crambe orientalis var. orientalis and Crambe tataria var. tataria collected from Kahramanmaras flora were morphologically examined and seed oil composition was determined. Volatile acid and fatty acid composition of seeds were examined with GC and GC/MS and the ratio of volatile acids to total oil was 3.49% in C. orientalis and 17.49% in C. tataria. The ratio of fatty acids to total oil was 92.03 and 67.28% in C. orientalis, and C. Tataria, respectively. The amount of erucic acid was 39.29% in C. orientalis and 29.87% in C. tataria. High linolenic acid (21.21%) and linoleic acid (12.42%) was found in C. orientalis oil, and high linolenic acid (15.01%) and linoleic acid (9.00%) was also found in C. tataria oil.     

大果白刺游离脂肪酸的柱前衍生高效液相色谱-荧光检测法分析

采用2-（11H-苯[a]咔唑）乙基对甲苯磺酸酯（BCETS）为柱前荧光衍生试剂,通过梯度洗脱使得18种脂肪酸在BDS-C8柱上得到良好的分离.方法应用于大果白刺不同部位中游离脂肪酸的分析,结果表明大果白刺的果皮果肉和叶子中均含有大量的不饱和脂肪酸,其总不饱和脂肪酸含量分别为70.74%和73.47%.大果白刺种子中不饱和脂肪酸的含量相对较少,仅占总脂肪酸含量的57.21%,其不饱和脂肪酸组成主要是C18∶1（油酸）和C18∶2（亚油酸）.其中,大果白刺的果皮果肉中,不饱和脂肪酸主要是C18∶1、C18∶2和C18∶3（亚麻酸）.其叶子中的不饱和脂肪酸主要是C18∶3,所占总脂肪酸比例为48.34%.首次对大果白刺中的脂肪酸进行了分析,可以为大果白刺在食品、药品中的进一步开发应用和质量控制提供一定的数据支持.     

Simultaneous analysis of glycolipids and phospholids molecular species in avocado (Persea americana Mill) fruit

The molecular species of phospholipids (PLs) and glycolipids (GLs) were simultaneously characterized in the pulp and almond of the avocado fruit (Persea americana Mill) of four varieties by means of high performance liquid chromatography-electrospray ionisation ion-trap tandem mass spectrometry. In the pulp, the predominant species of monoglycosyldiglycerides (MGD) were m/z 796.6 (oleic/linolenic and linoleic/linoleic acids) and m/z 800.4 (stearic/linoleic and oleic/oleic acids). One of the main diglycosyldiglycerides (DGD) both in the pulp and almond was m/z 958.5 (oleic/linolenic); however, the pulp was also rich of m/z 962.4 (oleic/oleic), whereas in the almond, m/z 934.5 (palmitic/linoleic and palmitoleic/oleic) and m/z 960.5 (oleic/linoleic and stearic/linolenic) were more abundant. In the almond, the main PL classes (phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI)) contained always palmitic/linoleic acids. Alpha-linolenic acid was contained as MGD (linolenic/linolenic) and DGD (linolenic/linolenic), more present in the pulp than in the almond. The major molecular species of glycocerebrosides (GCer) in the pulp and almond carried hydroxy-palmitic acid (C(16h:0))/4,8-sphyngadienine (d(18:2)).     

Evaluation of chemical constitute,fatty acids and antioxidant activity of the fruit and seed of sea buckthorn (Hippophae rhamnoides L.) grown wild in Iran

In this investigation, the chemical compositions of berries from sea buckthorn were studied. The amount of ascorbic acid and β-carotene determined by HPLC was 170 mg/100 g FW and 0.20 mg/g FW, respectively. Total phenols, anthocyanins, acidity and total soluble solids (TSS) contents were 247 mg GAE/100 g FW, 3 mg/L (cyanidin-3-glucoside), 5.32% and 13.8%, respectively. Fruit antioxidant activity determined by the ferric reducing ability of plasma (FRAP) method was 24.85 mM Fe/100 g FW. Results confirmed the presence of six dominant fatty acids (determined by GC) in fruit including linoleic (34.2%), palmitoleic (21.37%), palmitic (17.2%), oleic (12.8%), linolenic (5.37%) and stearic acid (1.67%). Five dominant fatty acids of the seeds were linoleic (42.36%), linolenic (21.27%), oleic (21.34%), palmitic (6.54%) and stearic acid (2.54%). The nitrogen content was 3.96%. The P, K, Ca, Mg, Fe, Zn, Mn, Cu, Cd and Cl contents of fruit were 491, 1674, 1290, 990, 291, 29.77, 108.37, 17.87, 0.021 and 2.18 mg/kg DW, respectively.     

Functional properties and fatty acids profile of different beans varieties

Dried seeds of four varieties of Phaseolus vulgaris, three of Vigna unguiculata ssp. unguiculata and two of Vigna angularis grown and marketed in Italy, Mexico, India, Japan, Ghana and Ivory Coast were analysed for fatty acids content. In oils from seeds of P. vulgaris, the main fatty acids were linolenic (34.7–41.5%) and linoleic (30.7–40.3%), followed by palmitic (10.7–16.8%). The first three aforementioned fatty acids in the lipid fraction of V. unguiculata varieties were 28.4, 28.7 and 26.2%, respectively; while in V. angularis varieties, main fatty acids were linoleic (36.4–39.1%) and palmitic (26.9–33.3%), followed by linolenic (17.9–22.2%). Statistical analyses indicate that botanical species play a rule in bean fatty acids distribution, while the same was not verified for geographical origin. Furthermore, the atherogenic index (AI) and the thrombogenic index (TI) were investigated for health and nutritional information. The results showed that these wide spread legumes have functional features to human health.     

Heterogeneous reactions of linoleic acid and linolenic acid particles with ozone: reaction pathways and changes in particle mass, hygroscopicity, and morphology

In this study, an electrodynamic balance (EDB) and a single particle Raman spectroscopic system were used to investigate the heterogeneous reactions of linoleic acid and linolenic acid with ozone under ambient temperatures (22-24 degrees C) and dry conditions (RH<5%). Raman characterizations provide evidence that ozone-induced autoxidation, in addition to direct ozonolysis, is a plausible pathway in the reactions between ozone and linoleic acid and linolenic acid particles. Furthermore, the significance of this specific oxidation pathway depends on the ozone concentrations used in the experiment. A low ozone concentration (approximately 200-250 ppb) with a longer exposure period (20 h) favors autoxidation but an extremely high ozone concentration (approximately 10 ppm) favors ozonolysis and forces most unsaturated fatty acids to react with ozone in a relatively short period of time. In the low ozone concentration experiments, the mass of the ozone-processed linoleic acid and linolenic acid particles increased by about 2-3% and 10-13%, respectively. In addition, the mass ratios (particle mass at RH approximately 85% to particle mass at RH<5%) of the ozone-processed linoleic acid and linolenic acid particles increased by about 2-3% and 3-4%, respectively. The morphology of the pure and ozone-processed linoleic acid and linolenic acid particles are compared, based on imagining and their light scattering patterns.     

GC Analysis of the Fatty Acid Composition of Yak Kidney

To obtain valuable information for development of potential commercial products from yak kidney, which is usually treated as waste, the fatty acid composition of kidneys from yak reared in Gansu province, China, was investigated by gas chromatography. Fifteen different fatty acids were identified. The major fatty acids are oleic acid (33.3%), stearic acid (20.2%), and palmitic acid (18.4%). More interestingly, several important and essential fatty acids were also identified, including conjugated linoleic acid (2.94%), omega-3 fatty acids, for example docosahexaenoic acid (1.47%), eicosapentaenoic acid (1.11%) and alpha linolenic acid (0.37%), and omega-6 fatty acids, for example arachidonic acid (2.86%) and linoleic acid (1.98%). The results show that the fatty acid composition of yak kidney is of reasonable value and is suitable for further development of possible commercial products. This is the first report of the fatty acid profile of yak kidney.

     

Glycolipids from Capsicum annuum

The component and fatty-acid compositions of glycolipids from seeds and pulp of Capsicum annuum (Solanaceae) were established. The dominant acid of seed galactolipids was found to be linoleic; of pulp, linolenic. The content of medium-chain acids 9:0-15:0 was greater in seed glycolipids than in the analgous component of pulp lipids.     

Separation of polyunsaturated fatty acid radicals by high-performance liquid chromatography with electron spin resonance and ultraviolet detection

In the reaction of soybean lipoxygenase (EC 1.13.11.12) with polyunsaturated fatty acids such as linoleic, linolenic and arachidonic acids, some radical species were detected using the electron spin resonance (ESR) spin-trapping technique. The radical species derived from the three polyunsaturated fatty acids were not distinguishable because the ESR spectra of the spin adducts of nitrosobenzene with their three radical species showed no difference in their hyperfine splittings. To overcome this defect of the spin-trapping technique, these spin-adducts were separated by employing high-performance liquid chromatography (HPLC) combined with ESR spectroscopy. The spin adducts were eluted from a C18 reversed-phase column in the order linolenic acid, linoleic acid and arachidonic acid. The half-lives of the spin adducts separated by HPLC-ESR were determined as linoleic acid 600 min, linolenic acid 360 min and arachidonic acid 160 min. The use of an ultraviolet detector together with the HPLC-ESR technique resulted in a 500-fold increase in sensitivity in the detection of the radical species.