A New Apocarotenoid of Capsicum annuum var. fasciculatum

Chemistry of Natural Compounds - A new apocarotenoid, fasciculatol (1) was isolated from the stems of Capsicum annuum var. fasciculatum (Sturtev.) Irish (Solanaceae). The structure of the new...     

Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells

Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.     

A new sesterterpenoid from the fruits of Phellodendron chinense var.glabriusculum Schneid

A new sesterterpenoid phellogine was isolated from the fruits of Phellodendron chinense var. glabriusculum Schneid. Its structure was elucidated on the basis of spectral analysis.     

Biyouyanagin A, an anti-HIV agent from Hypericum chinense L. var. salicifolium

[structure: see text] A structurally unique hydrophobic compound, biyouyanagin A, was isolated from the MeOH extract of the leaves of Hypericum chinense L. var. salicifolium. The structure of biyouyanagin A was elucidated on the basis of spectroscopic evidence. Biyouyanagin A showed a significant activity against HIV and inhibited cytokine production.     

薤中新的甾体皂苷类化学成分

从薤(Allium chinense G. Don)的乙醇提取物中分离得到6个新甾体皂苷类化合物, 通过波谱数据及理化性质分析, 鉴定其分别为5α-cholano-22,16-内酯-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(1)、 6-酮-5α-cholano-22,16-内酯-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(2)、 (25R)-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(3)、 (25R)-6-酮-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,22α,26-三醇-3-O-α-L-吡喃木糖基-(1→4)-β-D-吡喃葡萄糖苷(4)、 (25R)-6-酮-5α-呋喃甾烷-3β,22α,24β,26-四醇-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(5)和(25R)-5α-呋甾-2α,3β,22α, 26-四醇-26-O-β-D-吡喃葡萄糖苷(6). 化合物1和2的皂苷元骨架在天然产物中首次分离得到. 选用H₂O₂诱导PC12细胞神经氧化损伤模型, 初步考察了6种新的呋甾型化合物的抗氧化活性, 实验结果表明, 化合物3对由H₂O₂诱导的细胞氧化损伤有显著的保护效果.     

New cytotoxic steroid from Stachyurus imalaicus var. himalaicus

A phytochemical study of the ethanolic extract of Stachyurus imalaicus var. himalaicus was undertaken and as a result a new polyoxygenated steroid, named stachsterol ((20S)-20, 25-dihydroxy-4-cholesten-3-one, 1) and three known ecdysteroids, 20-hydroxyecdysone (2), 20-hydroxyecdysone-20, 22-monoacetonide (3) and polypodine B-20,22-monoacetonide (4), were isolated. Their structures were elucidated by spectroscopic methods, including UV, NMR, MS and HR-MS. The purified product 1 was found to have in vitro cytotoxic activity against human Hela cell lines with an IC50 value of 2.5 microg/mL. This is the first time that phytoecdysteroids have been found in the genus Stachyurus.     

Three New Diterpenoids from Rabdosia lophanthoides var. gerardiana

Three new diterpenoids, together with three known ones, were isolated from the air‐dried whole herbs of Rabdosia lophanthoides var. gerardiana. The structures of the new diterpenoids were established as 3,4‐dihydro‐11‐hydroxy‐10‐(1‐hydroxy‐1‐methylethyl)‐2,2,6‐trimethylnaphtho[1,8‐bc]oxocin‐5(2H)‐one ( 1 ), 11,12,15‐trihydroxyabieta‐5,8,11,13‐tetraen‐7‐one ( 2 ), (2R,3S,4S,4aR,8S,9aS,13aS,16aS)‐3,4,4a,8,9,9a,10,11,12,13,14,16a‐dodecahydro‐2‐(hydroxymethyl)‐6,6,10,10‐tetramethyl‐2H‐benzo[4,5]cyclohepta[1,2‐h]pyrano[2,3‐b][1,4]benzodioxepine‐3,4,8,13a,15(6H)‐pentol ( 3 ) by spectroscopic methods, including extensive 1D‐ and 2D‐NMR analyses. The structures of the known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature.     

A New Diterpene from Spiraea japonica var.ovalifolia

A new diterpenoid,15-0-accetylspiraminol(1),was isolated from the aerial parts of Spireae japonica L.f.var.ovalifolia.The structure was charaterized mainly based on spectral analysis.     

Four New Triterpenes from Anchusa azurea var. azurea

Four new triterpene glycosides, named oleanazuroside 1 ( 1 ), oleanazuroside 2 ( 2 ), ursolazuroside 1 ( 3 ), and ursolazuroside 2 ( 4 ), together with the seven known compounds 5 – 11 , were isolated from the MeOH extract of the aerial parts of Anchusa azurea Miller var. azurea. Their structures were elucidated by means of spectroscopic evidence (UV, IR, MALDI‐MS, and 1D‐ and 2D‐NMR). The radical‐scavenging activities against 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) of the BuOH extract and of 8 and 10 were very strong (Table 5).     

New Diterpenoid Alkaloids from Spiraea fritschiana var. parvifolia

SPiraeafritschianavar.parvdoliadistributedinthenorthofChinaisoneofthethreevarietiesofSfritschiana'.RecentlythreeCZohetisine-typediterpenoidalkaloids,spiradineD(3)',spirafineill(l)andspirafine11(2)wereisolatedfromitsroots.PreliminarypharmaceuticalinvestigationrevealedthatspirdineDhadanti-PAFactivity.CompoundIisobtainedinformofcolorlessneedlecrystals,m.p.192-193"C;[a].'=3--46.07(c2.0,C,H,N).ItsmolecularformulawasdeterminedasC,,H,,NO,byHRMS(341.2279,cafe.:341.2355).ItsiR(KBr)spectrumex…     

Two New Compounds from Hemsleya penxianensis var.gulinensis

Two new compounds,oleanolic acid 28-O- β-D-glucopyranosyl-3-O-α-L-arabinopyranosyl-(1→3)-(6′-butyl ester)-β-D-glucuropyranoside 1,oleanolic acid 28-O-β-D-glucopyranosyl(1→6)-β-D-glucopyranoside 2 have been isolated from the roots of Hemsleya penxianensis var.gulinensis.Their structures were determined on the bases of the spectral and chemical evidences.     

New Diterpenoid Alkaloids from Spiraea japonica var. Ovalifolia

In the investigation of the chemical constituents of Spireae japonica L. f. var. ovalifolia (Rosaceae), a shrub originated in Songming, Yunnan, five new diterpenoid alkaloids, 19-O-deethylspiramine N (1), deacetylspiramine S (2), spiramidine A (3), spiramidine B (4) and deacetylspiramine F (5), were isolated from aerial parts of the plant and structurally elucidated. Compound 1, a white amorphous powder with [(]+17.99 (c, 0.26, MeOH ), was determined to have the molecular formula of C20H…     

New Polyoxygenated Triterpenoids from Stachyurus himalaicus var. himalaicus

Two new polyoxygenated triterpenoids, stachlic acid A (= (2α,3β)‐2,3,23,29‐tetrahydroxyolean‐12‐en‐28‐oic acid; 1 ) and stachlic acid B (= (2α,3α)‐2,29‐dihydroxy‐3,23‐[(1,1‐dimethylmethylene)dioxy]olean‐12‐ene‐28‐oic acid; 2 ), were isolated from Stachyurus himalaicus var. himalaicus. Their structures were established by means of extensive spectroscopic studies and chemical evidence. The purified product 1 was found to have moderate in vitro cytotoxic activity against human Hela cells.     

New Diterpene Alkaloids from Aconitum sungpanense var. leucanthum

Five new C₁₉ diterpene alkaloids, leucanthumsines A ( 1 ), B ( 2 ), C ( 3 ), D ( 4 ), and E ( 5 ), were isolated from the Chinese medicinal herb Aconitum sungpanense var. leucanthum, together with the known C₁₉ diterpene alkaloids pseudaconine, neoline, 1‐O‐methyldelphisine, crassicaudine, chasmanine, talatisamine, indaconitine, ezochansmanine, and leueantine D. The structures of these new alkaloids were elucidated by HR‐MS and advanced NMR methods, including ¹H‐ and ¹³C‐NMR (DEPT), ¹H,¹H‐COSY, HMQC, and HMBC experiments.     

New Monoterpenoids from Fruits of Gardenia jasminoides var. radicans

One new lactone, cyclopentanepyrone A ( 1 ), and two new monoterpenoids, gardeterpenone A ( 2 ) and jasminoside V ( 3 ), were isolated from the fruits of Gardenia jasminoides var. radicans, along with four known monoterpenoids, 4 – 7 , which were isolated from this plant for the first time. The structures of the isolates were elucidated by extensive spectroscopic studies, including UV, IR, 1D‐ and 2D‐NMR, ESI‐MS, HR‐ESI‐MS, and CD experiments.     

New megastigmane glucosides from Excoecaria cochinchinensis LOUR. var. cochinchinensis

Two new megastigmane glucosides, called excoecariosides A and B were isolated from the leaves of a medicinal Vietnamese plant, Excoecaria cochinchinensis LOUR. var. cochinchinensis (Euphorbiaceae) together with seven known compounds. Their structures were elucidated by spectroscopic analyses and chemical reactions including the modified Mosher's method.