苦丁茶冬青挥发油成分的GC-MS分析

采用乙醚超声萃取-水蒸气蒸馏方法提取苦丁茶冬青的挥发油，用气相色谱-质谱联用分析，鉴定了挥发油中的57个成分，其中主要成分是醇、醛、酮、醚、脂肪酸、脂肪酸酯及含氧萜类等化合物。     

花椒挥发油的化学成分分析

1　引　　言花椒 (ＺａｎｔｈｏｘｙｌｕｍｂｕｎｇｅａｎｕｍＭａｘｉｎ)属于芸香科落叶灌木植物 ,具有浓烈芳香 ,是主要的调味香料 ,在烹调中常用作佐料 ,又可防腐。花椒还属于中国传统的有效高价值中药 ,它性热 ,味辛、入脾经胃 ,能散寒祛湿 ,止痛杀虫 ,并可除六腑寒冷。主治慢性肠胃炎 ,胃腹冷痛及慢性关节炎、肌肉风湿痛等疾病。国外对花椒类植物也进行了一定研究工作 ,据有关报道 ,花椒属植物的提取物或挥发油在抗病毒、杀病菌、微生物等方面有显著的功效。花椒粗提物有较强的杀虫、杀菌作用 ,它对玉米象、赤拟谷盗、储粮曲霉和青霉等…     

佛手挥发油的化学成分

用色谱－质谱－计算机联用法对佛手挥发油的化学成分和相对含量进行了系统分析,得到６７种组分,鉴定出３１个组分,主要组分是柠檬烯（４８．４％）、１－甲基－２－（１－甲乙基）－苯（３０．８％）。     

艾叶挥发油的化学成分

采用水蒸气蒸馏法从艾叶中提取挥发油,试用不同类型的毛细管柱进行分析,找出最佳分析条件,共分离出59个蜂,用归一化法测定其相对含量,并用气相色谱－质谱法对化学成分进行鉴定,共鉴定了51个万分,占挥发油总成分的86％以上。     

大萼香茶菜挥发油化学成分的气相色谱/质谱法分析

采用常规水蒸气蒸馏法提取出大萼香香茶菜挥发油，经气相色谱－质谱联机分析，分离出116个峰，确认了其中60种成分，所鉴定的组分占总峰面积的75．77％。     

大叶冬青挥发油化学成分的GC-MS分析

采用气相色谱-质谱联用法对大叶冬青挥发油的化学成分进行研究,鉴定出大叶冬青挥发油中含醇、酮、酰胺及含氧萜类等71个化学成分,运用峰面积归一化法测定各化学成分的相对百分含量,已鉴定的化学成分占挥发油总量的96.16%.     

白千层挥发油化学成分分析

用毛细管气相色谱、气相色谱－ 质谱－ 计算机分析技术, 对植物白千层枝叶挥发油化学成分进行了分析研究, 从这种植物挥发油中分离出８０ 个以上的组分, 确认了其中的３５ 种成分, 所鉴定的组分占挥发油色谱总峰面积的９０ ％ 以上。     

气相色谱-质谱法分析山柰挥发油化学成分

采用水蒸气蒸馏法从山柰中提取挥发油。采用不同类型的毛细管桩进行分析,找出最佳分析条件,用归一化法测定其质量分数用并用相色谱－质谱法对化学成分进行鉴定。共鉴定了３４种成分,占挥发油总成分的９１％以上。     

气相色谱-质谱联用分析花椒挥发油的成分

 采用气相色谱 质谱联用技术对陕西富平产花椒挥发油的成分进行了分析 ,分离出了 17个峰 ,鉴定出了相对峰面积大于 1 0 %的组分共 16个 ,用峰面积归一化法定量测定了已确定组分的相对含量 ,其占挥发油总质量的95 %以上。     

气相色谱-质谱法测定艾叶挥发油中化学成分

采用水蒸气蒸馏法提取艾叶的挥发油,用气相色谱-质谱法分离和鉴定挥发油成分,并用归一化法测定其相对含量。共分离出76个组分,鉴定出59种化合物。其含量占总挥发油组分峰面积的94.3%。贵州遵义产艾叶主要挥发油成分及其含量为1,8-桉叶油素(22.19%)、樟脑(10.39%)、绿花白千层醇(6.57%)、蒿醇(4.95%)、L-龙脑(4.88%)、α-松油烯(3.98%)、蒿酮(3.44%)、顺式桧烯水合物(3.36%)、4-松油醇(2.68%)、菊油环酮(2.51%)、β-崖柏酮(2.41%)、1-松油醇(2.32%)和丁香酚(2.26%)等。     

Two Novel Quassinoid Glycosides with Antiviral Activity from the Samara of Ailanthus altissima

Phytochemistry investigations on Ailanthus altissima (Mill.) Swingle, a Simaroubaceae plant that is recognized as a traditional herbal medicine, have afforded various natural products, among which C₂₀ quassinoid is the most attractive for their significant and diverse pharmacological and biological activities. Our continuous study has led to the isolation of two novel quassinoid glycosides, named chuglycosides J and K, together with fourteen known lignans from the samara of A. altissima. The new structures were elucidated based on comprehensive spectra data analysis. All of the compounds were evaluated for their anti-tobacco mosaic virus activity, among which chuglycosides J and K exhibited inhibitory effects against the virus multiplication with half maximal inhibitory concentration (IC₅₀) values of 56.21 ± 1.86 and 137.74 ± 3.57 μM, respectively.     

Two new compounds from the fruit of Ailanthus altissima

A new phenolic derivative, 4-hydroxyphenol-1-O-[6-O-(E)-feruloyl-β-d-glucopyranosyl]-(1→6)-β-d-glucopyranoside (1), and a new terpenylated coumarin, named altissimacoumarin H (2) were identified from the fruit of Ailanthus altissima (Mill.) Swingle (Simaroubaceae), together with ten known compounds (3–12), including two coumarins and eight phenylpropanoids. Their structures were determined on the basis of chemical method and spectroscopic data. Antiviral effect against Tobacco mosaic virus (TMV) of all the compounds obtained were evaluated using leaf-disc method.     

艾纳香挥发油化学成分的气相色谱 -质谱分析

由黔产艾纳香（Blumea balsamifera DC.)的叶提取挥发油,分出结晶后的母液,用气相色谱－质谱进行定性分析和峰面积相对含量的测定,鉴定出41个化学成分;主要成分为L－龙脑、樟脑、β-石竹烯、桉叶油醇、古芸烯、芳樟醇等。     

Heat shock,visible light or high calcium augment the cytotoxic effects of Ailanthus altissima (Swingle) leaf extracts against Saccharomyces cerevisiae cells

To gain new insight into the antimicrobial potential of Ailanthus altissima Swingle, ethanol leaf extracts were evaluated for the antifungal effects against the model yeast Saccharomyces cerevisae. The extracts inhibited the yeast growth in a dose-dependent manner, and this effect could be augmented by heat shock, exposure to visible light or exposure to high concentrations of Ca²⁺. Using transgenic yeast cells expressing the Ca²⁺-dependent photoprotein, aequorin, it was found that the leaf extracts induced cytosolic Ca²⁺ elevation. Experiments on yeast mutants with defects in Ca²⁺ transport demonstrated that the cytotoxicity of the A. altissima leaf extracts (AaLEs) was mediated by transient pulses of Ca²⁺ ions which were released into the cytosol predominantly from the vacuole. The investigation of the antifungal synergies involving AaLEs may contribute to the development of optimal and safe combination therapies for the treatment of drug-resistant fungal infections.     

北苍术和茅苍术挥发油成分的比较

采用气相色谱-质谱联用技术分析了北苍术和茅苍术的挥发油成分,分别鉴定出47和50种化学成分,并测定了其相对含量。 采用色谱指纹图谱八强峰法和分区法,根据色谱峰的保留时间把总离子流色谱图分为5个区,比较了北苍术和茅苍术的挥发油成分。 结果表明,在总离子流色谱图Ⅳ和Ⅴ区中,从北苍术和茅苍术中均检出苍术的主要药效成分β-桉叶油醇、苍术酮和苍术素,且相对含量较高。 这表明《中国药典》将北苍术、茅苍术统称为苍术具有一定的科学性。     

阿育魏实挥发油成分的GC-MS分析

采用水蒸气蒸馏的方法提取了阿育魏实挥发油,并利用气相色谱一质谱联用技术对其化学成分做了分析,共鉴定出麝香草酚、β-伞花烃、萜品烯、α-崖柏烯、β-月桂烯和香芹酚等53个化合物。研究阿育魏实挥发油的成分,对民族药品质提升及原料的质量保证具有十分重要的意义。     

Molar Mass Distribution and Chain Conformation of Polysaccharides from Fortunella margarita （Lour.） Swingle

In order to study the effects of different extraction methods on the molar mass distribution and chain conformation of Fortunella margarita(Lour.) Swingle polysaccharides(FP), we used extraction by hot water(WFP), ultrasonic-assisted treatment(UFP), microwave-assisted treatment(MFP) and ultrasonic/microwave-assisted treatment(UMFP), and then Fourier transform infrared(FT-IR) spectroscopy as well as 1H and 13 C nuclear magnetic resonance(NMR) spectroscopy to characterize the structural properties of FP extracted. The molar weight(Mw), polydispersity index(Mw/Mn), root-mean-square(RMS) turning radius(Rg), molar mass distribution and chain conformation of FP were studied systematically using size-exclusion chromatography(SEC), multi-angle laser light-scattering(MALLS) and refractive index(RI). WFP, UFP, MFP and UMFP are all typical carbohydrates according to 1H NMR, 13 C NMR and FT-IR measurements. The type of glycosidic linkage is mainly a β-glycosidic bond with a small amount of α-glycosidic bond. The results obtained by the SEC-MALLS-RI system showed the molar masses of WFP and UMFP were distributed mainly in the range of 5.0×106~1.0×107 g·mol–1 and they accounted for 57.80% and 56.84% of total FP, respectively. The molar masses of UFP and MFP were distributed mainly in the 1.0×106~5.0×106 g·mol–1 range, which accounted for 38.24% and 52.39% of FP, respectively. WFP and UMFP in water were uniform spherical polymers; UFP and MFP were typical highly branched polymers and the degree of branching for MFP was higher compared to UFP. These results indicated the ultrasonic- and microwave-assisted extraction methods caused a significant decrease of the molar mass of FP but the ultrasonic/microwave synergistic extraction method had no effect.     

Two new homoisoflavanones from Polygonatum odoratum (Mill.) Druce

<正>Two new homoisoflavanones were isolated from the rhizomes of Polygonatum odoratum(Mill.) Druce and their structures were elucidated as(3R)-5,7-dihydroxy-8-methoxy-3-(4-methoxybenzyl)-6-methylchrom-an-4-one(1) and(3R)-5,7,8-trihydroxy-3-(4- hydroxybenzyl)-6-methylchroman-4-one(2),on the basis of spectral analysis.     

气相色谱-质谱法测定阿尔泰狗娃花挥发精油中化学成分

采用水蒸气回流法提取阿尔泰狗娃花挥中挥发精油,用气相色谱-质谱法分离和鉴定阿尔泰狗娃花挥发精油中化学成分,用归一法测定其相对含量,共分离出50种化学成分,鉴定了39种化学成分,约占总成分相对含量的84.7%。     

Components of Volatile Oil from Water-caltrop and Their Anti-tumor Effect in vitro

The volatile oil was extracted from water caltrop by steam distillation; it was then analyzed by GC-MS to obtain 16 components, 8 of which were identified. Apocynin was the most abundant one, accounting for 81.41% of the total oil. The in vitro inhibitory effects of the volatile oil on SMMC-7721, MCF-7, Hela, HL-60 cells, and human peripheral blood mononuclear celIs(PBMC) were investigated via the MTT method. The morphological changes of the tumor cells were observed and the apoptosis of HL-60 cells was detected by flow cytometry. The proliferation of the tumor cells could be significantly inhibited and the apoptosis of HL-60 cells could be induced by the volatile oil. The proliferation inhibition effect of the volatile oil on HL-60 tumor cells and the induction of the apoptosis of HL-60 cells had dose-dependent feature.