食管癌患者肿瘤组织中硒含量的调查测定

应用2，3-二氨基萘荧光测定法对36例食管癌患者癌组织与癌旁组织及血清中硒的含量进行了测定。结果表明，癌组织中硒含量平均为1．325±0．81μg／g干重，癌旁组织为0．893±0．68μg／g干重，两者差异显著（P＜0．05）；血清中硒含量为0.6033±0．0520μmol／L，对照组20例献血员血清为0．8034±0．0650μmol／L，亦有显著性差异（P＜0．05）。提示硒在抗肿瘤的过程中有一定作用。     

氢化物发生电感耦合等离子体原子发射光谱法同时测定水和生物样品中…

研究了一种小型同心氢化物发生配置一个气液分离器后与多道ＩＣＰ－ＡＥＳ联用，同时测定水和生物样品中砷、铋、锑、硒的方法。检出限为砷０．４μｇ／Ｌ，铋０．５μｇ／Ｌ，锑１．４μｇ／Ｌ，硒０．５μｇ／Ｌ，相对标准偏差为砷２．７％，铋１．５％，锑２．７％，硒１．９％。用本法测定美国和国家标准物质中的氢化元素，结果满意。     

食管贲门癌、肺癌病人血清锌变化与手术创伤愈合的关系

对５０例胸外科手术的癌症病人进行了临床观察，用日立ＡＡ－６７０型原子吸收分光光度计测血清锌含量．结果表明．食管资门癌、肺癌病人均有不同程度锌缺乏．实验组血清锌均值为６６．３５±１８．１６μｇ／ｄＬ，对照组为６６．２０±７．６５μｇ／ｄＬ．实验组补锌后，血清锌均值升高为７２．７２±２１．１２μｇ／ｄＬ．实验组手术后无切口愈合不良及吻合口瘘并发症发生，对照组切口愈合不良６例（其中２例切口完全裂开）（Ｐ＜０．０１），吻合口瘘４例（均伴有切口愈合不良）（Ｐ＜．００５）．两组间有显著差异．锌在创伤愈合过程中确有重要的促进作用．     

离子交换分离—氢化物原子吸收光谱法测定紫铜中微是量硒

采用阳离子交换树脂离子分离铜，发生氢化物原子吸收光谱法测定硒，上柱溶液酸度在０－７％，硒的回收率在９６－１０１％，过柱溶液中铜的浓度低于０．０１μｇ／ｍＬ。方法检出限为５×１０＾－５％，分析紫铜中４．４×１０＾－４％的硒，相对标准偏差为３％。     

长光路光度法测定中药材中痕量硒

本文研究了用长光路光度法测定痕量硒。在酸性溶液中，Ｓｅ（Ⅳ）催化ＫＣｌＯ３氧化苯肼成偶氮离子，继而与变色酸生成红色偶氮化合物。硒含量在１．０×１０－９～２．０×１０－７ｇ／ｍＬ范围内遵守比尔定律。巯基棉分离后，测定中药材中总硒，获得满意结果。     

催化动力学法测定痕量硒（Ⅳ）

１引言利用催化动力学光度法测定痕量硒已有报道，但都存在Ｆｅ３＋等一些共存离子干扰等问题。作者通过实验观察到，在盐酸介质中，Ｓｅ（Ⅳ）能强烈催化氯酸钾氧化甲苯胺兰褪色反应，据此建立了催化动力学光度法测定痕量硒的一种新方法。方法具有体系简单稳定，选择性好等特点，检出限为８４ｘ１０－７ｇ／Ｌ，线性范围为０～９×１０－７ｇ／２５ｍＬ。直接用于水；人发、面粉和牛奶等食品样中的痕量硒（Ⅳ）测定，结果较好。２实验方法在两支相同的２５ｍＬ比色管中，一支加入０．５μｇ的标准硒（Ⅳ）工作溶液，另一支不加。然后依次分…     

利用微波消解和高效液相色谱荧光检测法测定环境水样中痕量硒

基于微波消解和正相液相色谱－荧光检测测硒法,实现了对环境水样中亚纳克级硒的测定。考察了ＨＮＯ３和Ｈ２ＳＯ４－Ｈ２Ｏ２两种消化体系及其工作条件,方法检出限分别可达 ７．２和 １０．４ ｐｇ Ｓｅ,对标准参考物质的回收率分别为９９．４±２．０和９７．８±２．５％,在０～１．５ｎｇＳｅ范围内有线性关系（Ｒ２＜０．９９６）。对北京地区 ４种不同类型水体水样含硒量进行了分析测定（２ｍＬ样品）,其浓度范围为 ０．２０～０．９０μｇ／Ｌ,标准加入回收率为９９．１％～１０３．４％。     

离子交换分离－氢化物原子吸收光谱法测定紫铜中微是量硒

采用阳离子交换树脂离子分离铜，发生氢化物原子吸收光谱法测定硒。上柱溶液酸度在０～７％，硒的回收率在９６～１０１％，过柱溶液中铜的浓度低于０．０１μｇ／ｍＬ。方法检出限为５×１０￣（－５）％，分析紫铜中４．４×１０￣（－４）％的硒，相对标准偏差为３％。     

硒－硫氰酸钾－罗丹明B－明胶－OP体系分光光度法测定微量硒

硒的分光光度法一般采用硒试剂作显色剂，其灵敏度低，操作繁琐。硒－硫氰酸盐－丁基罗丹明Ｂ－吐温－２０体系分光光度法测定微量硒的灵敏度较高￣［１］，在该文的基础上，本人研究出硒－硫氰酸钾－罗丹明Ｂ－明胶－ＯＰ体系分光光度法，具有灵敏度高，稳定性好，操作简便等特点。研究结果表明：在明胶－乳化剂ＯＰ存在下，控制溶液ｐＨ３．８，用硫脉作还原剂，ＥＤＴＡ为掩蔽剂，硒与硫氰酸钾及罗丹明Ｂ生成三元配合物，配合比Ｓｅ（Ⅳ）：ＣＮＳ￣－：ＲＢ＝１：６：４，最大吸收波长为６０６ｎｍ，硒含量在０～３μｇ／２５ｍＬ服从比尔定律，ε＿（６０６）＝７．９×１０￣５Ｌ．ｍｏｌ￣（－１）·ｃｍ￣（－１）。用于矿泉水中微量硒的测定。     

氢化物电热原子吸收法测定人血清中的硒

应用氢化物电热原子化装置测定了血清中的硒。采用这种技术可减少试样的基体干扰，提高分析灵敏度，而且具有良好的线性关系。方法测定灵敏度为１．２μｇ／Ｌ（１％吸收），检出限０．４μｇ／Ｌ变异系数６．３％，具有血量少、成本低、方法简便、精度好等优点。     

人体微量元素硒营养

硒是人体必需的微量元素。环境系统中的硒含量差异较大，缺硒现象普遍。利用天然食品摄取硒营养是安全，有效，可行的途径。     

Ag_2Se纳米材料的制备方法

对纳米Ag2Se的化学制备方法进行了概述,着重介绍了模板法、声化学法、水(溶剂)热法、光照合成法、微乳状液法等;展示了新近得到的不同形貌和性质的As2Se纳米晶体;并对其发展趋势和应用前景进行了展望.     

Se和Se@C纳米棒的选择合成

通过调节葡萄糖浓度,采用十六烷基三甲基溴化铵(CTAB)辅助水热法选择合成了Se和Se@C纳米棒,并用扫描电镜(SEM)、透射电镜(TEM)、粉末X射线衍射(XRD)和X射线能谱分析(EDS)对产物进行了表征。结果表明,产物Se纳米棒直径200～300 nm,长达数微米;Se@C纳米棒长达数微米,内核直径200～00 nm,壳厚约30 nm。初步探讨了Se和Se@C纳米棒的生长机制。     

浙江长兴富硒农产品开发情况调查

对浙北长兴水口富硒土壤开发情况调研表明,该地土壤理化性质优越,硒含量高,生态环境优良。通过精心耕种,合理施肥,科学管理,所产农产品一般硒含量高、品质好、重金属低、社会经济效益好,为全省天然富硒农产品的规模生产提供了良好示范。     

血硒与肺癌

硒是谷胱甘肽过氧化物酶（ＧＳＨ－ＰＸ）的组成成分，是人体生命活动所必需的微量元素，本文主要就血硒的流行病学研究、人体硒状况的估价、硒的抗癌机制以及血硒在肺癌诊断和防治中的意义等方面作一概述。     

Flow injection for the determination of Se(IV) and Se(VI) by hydride generation atomic absorption spectrometry with microwave oven on-line prereduction of Se(VI) to Se(IV)

An on-line flow injection system has been developed for the selective determination of Se(IV) and Se(VI) in citric fruit juices and geothermal waters by hydride generation atomic absorption spectrometry with microwave-aided heating prereduction of Se(VI) to Se(IV). The samples and the prereductant solutions (4 mol l⁻¹ HCl for Se(IV) and 12 mol l⁻¹ HCl for Se(VI)) which circulated in a closed-flow circuit were injected by means of a time-based injector. This mixture was displaced by a carrier solution of 1% v/v of hydrochloric acid through a PTFE coil located inside the focused microwave oven and mixed downstream with a borohydride solution to generate the hydride. The linear ranges were 0–120 and 0–100 μg l⁻¹ of Se(IV) and Se(VI), respectively. The detection limits were 1.0 μg l⁻¹ for Se(IV) and 1.5 μg l⁻¹ for Se(VI). The precision (about 2.0–2.5% RSD) and recoveries (96–98% for Se(IV) and 94–98% for Se(VI)) were good. Total selenium values were also obtained by electrothermal atomic absorption spectrometry which agreed with the content of both selenium species. The sample throughput was about 50 measurements per hour. The main advantage of the method is that the selective determination of Se(IV) and Se(VI) in citric fruit juices and geothermal waters is performed in a closed system with a minimum sample manipulation, exposure to the environment, minimum sample waste and operator attention.     

Sequential determination of Se(IV) and Se(VI) by flow injection-hydride generation-atomic absorption spectrometry with HCl/HBr microwave aided pre-reduction of Se(VI) to Se(IV)

In this study a flow injection (FI) system used in conjunction with hydride generation (HG), atomic absorption spectrometry (AAS) and microwave (MW) aided pre-reduction of selenite (Se(IV)) to selenate (Se(IV)) with HCl:HBr has been developed in order to differentiate both inorganic selenium species. As full control of the MW reduction step is possible, the experimental approach allows the use of milder acidic conditions (10% v/v of HCl and HBr) than those conventionally accomplished with hydrochloric acid alone (≥50% v/v). Experimental parameters were optimized by the univariate optimization method. In either case, the linear range was from 1.0 to 30 μg l⁻¹. The detection limits based on 3σ of the blank signal were 0.25 μg l⁻¹ for Se(IV) and 0.30 μg l⁻¹ for Se(VI). The reproducibility, about 3% RSD and recoveries of different amounts of Se(VI) and Se(IV) added to water and orange juice samples (97–103%) were good. The main advantage of the proposed method is that the sequential determination of Se(IV) and Se(VI) is performed at a high sampling frequency (ca. 50 samples per h) in a closed system without Se losses, and with a minimum sample waste, operator attention, and sample manipulation.     

膳食中硒的生物利用率

探讨了影响膳食中硒生物利用率的各种因素，提出了应重视有机硒的开发与利用。     

温莪术中As、Se含量的测定

对温莪术中As、Se两种元素的含量采用HG-ICP-AES法进行了测定分析。结果表明,温莪术全草中具有较高的Se元素含量和很低的As元素含量,从As和Se两元素含量的角度可见,温莪术具有一定的药用价值。并将温莪术经超声煎煮不同时间后得到的药液、药渣进行对比,发现As含量均很低,而Se含量在药渣中显著高于药液。