High-frequency audiometric assessment of a young adult population

The hearing thresholds of 37 young adults (18-26 years) were measured at 13 frequencies (8, 9,10,...,20 kHz) using a newly developed high-frequency audiometer. All subjects were screened at 15 dB HL at the low audiometric frequencies, had tympanometry within normal limits, and had no history of significant hearing problems. The audiometer delivers sound from a driver unit to the ear canal through a lossy tube and earpiece providing a source impedance essentially equal to the characteristic impedance of the tube. A small microphone located within the earpiece is used to measure the response of the ear canal when an impulse is applied at the driver unit. From this response, a gain function is calculated relating the equivalent sound-pressure level of the source to the SPL at the medial end of the ear canal. For the subjects tested, this gain function showed a gradual increase from 2 to 12 dB over the frequency range. The standard deviation of the gain function was about 2.5 dB across subjects in the lower frequency region (8-14 kHz) and about 4 dB at the higher frequencies. Cross modes and poor fit of the earpiece to the ear canal prevented accurate calibration for some subjects at the highest frequencies. The average SPL at threshold was 23 dB at 8 kHz, 30 dB at 12 kHz, and 87 dB at 18 kHz. Despite the homogeneous nature of the sample, the younger subjects in the sample had reliably better thresholds than the older subjects. Repeated measurements of threshold over an interval as long as 1 month showed a standard deviation of 2.5 dB at the lower frequencies (8-14 kHz) and 4.5 dB at the higher frequencies.     

Normative thresholds in the 8- to 20-kHz range as a function of age

Using a prototype high-frequency audiometer, auditory thresholds in the 8- to 20-kHz range were obtained from 240 subjects ranging in age from 10-60 years. These measurements were obtained in interest of developing a normative database for frequencies above 8 kHz, and to evaluate intersubject variability as a function of age. An analysis of variance (ANOVA) revealed significant effects of frequency, age, and sex, and a significant frequency-by-age interaction. The largest changes in sensitivity with age occurred between 40 to 59 years. Below approximately 15 kHz, the intersubject variability of threshold estimates increased as a function of both age and frequency. Further analysis revealed that the age-related changes in variability were related to absolute thresholds rather than to age per se. When data are converted to dB HL (relative to the youngest group tested), the region of maximum hearing loss shifts to lower frequencies with increasing age, and threshold shifts with age are greatest in the 13- to 17-kHz range.     

Noise level, inner hair cell damage, audiometric features, and equal-energy hypothesis

Rabbits were exposed to 2- to 7-kHz noise either for a short duration at a high sound-pressure level (15 or 30 min at 115 dB SPL), or a long duration at a low level (512 h at 85 dB SPL). The high-level exposure produced a hearing loss in the frequency range 2-6 kHz, whereas the low-level exposure gave maximum hearing loss at 12-20 kHz. The 115-dB exposure caused significantly more damage to inner hair cells than the 85-dB exposure. The implications of the present results for evaluating audiograms, equal-energy hypothesis, risk criteria, and subjective auditory features are pointed out.     

Detection of temporal gaps in bandlimited noise: effects of variations in bandwidth and signal-to-masker ratio

Thresholds were measured for the detection of a temporal gap in a bandlimited noise signal presented in a continuous wideband masker, using an adaptive forced-choice procedure. In experiment I the ratio of signal spectrum level to masker spectrum level (the SMR) was fixed at 10 dB and gap thresholds were measured as a function of signal bandwidth at three center frequencies: 0.4, 1.0, and 6.5 kHz. Performance improved with increasing bandwidth and increasing center frequency. For a subset of conditions, gap threshold was also measured as bandwidth was varied keeping the upper cutoff frequency of the signal constant. In this case the variation of gap threshold with bandwidth was more gradual, suggesting that subjects detect the gap using primarily the highest frequency region available in the signal. At low center frequencies, however, subjects may have a limited ability to combine information in different frequency regions. In experiment II gap thresholds were measured as a function of SMR for several signal bandwidths at each of three center frequencies: 0.5, 1.0, and 6.5 kHz. Gap thresholds improved with increasing SMR, but the improvement was minimal for SMRs greater than 12-15 dB. The results are used to evaluate the relative importance of factors influencing gap threshold.     

The reliability of auditory thresholds in the 8- to 20-kHz range using a prototype audiometer

This study was designed to evaluate both intra- and intertester reliability of auditory thresholds in the 8- to 20-kHz range using a recently developed high-frequency audiometer [Stevens et al., J. Acoust. Soc. Am. 81, 470-484 (1987)]. With this device, signals from a high-frequency transducer are introduced into the ear canal via a plastic tube. A calibration function is calculated for each ear and used to estimate the sound-pressure level (SPL) at the tympanic membrane. Twenty normal-hearing listeners were tested four times, twice by each of two examiners. In the higher frequencies, accurate calibration functions could not be obtained for many subjects; in these cases, values extrapolated from lower frequencies were used to estimate SPL. Findings reveal that the standard error of measurement for both intra- and intertester measures increases as a function of frequency. Intertester variability was only slightly higher than intratester variability. In most cases, variability of threshold estimates in dB SPL was higher than that observed for the uncorrected attenuator settings. Exclusion of extrapolated values improved reliability substantially.     

Amplitude modulation thresholds in chinchillas with high-frequency hearing loss

Estimates of auditory temporal resolution were obtained from normal chinchillas using sinusoidally amplitude modulated noise. Afterwards, the animals were exposed to noise whose bandwidth was progressively increased toward the low frequencies in octave steps. The first exposure was to an octave band of noise centered at 8 kHz. Three additional octave bands of noise were subsequently added to the original exposure in order to progressively increase the extent of the high-frequency hearing loss. The first exposure produced a temporary hearing loss of 50 to 60 dB near 8 kHz and elevated the amplitude modulation thresholds primarily at intermediate (128 Hz) modulation frequencies. Successive noise exposures extended the temporary hearing loss toward lower frequencies, but there was little further deterioration in the amplitude modulation function until the last exposure when the hearing loss spread to 1 kHz. The degradation in the amplitude modulation function observed after the last exposure, however, was due to a reduction in the sensation level of the test signal rather than to a decrease in the hearing bandwidth. The results of this study suggest that the high-frequency regions of the cochlea may be important for temporal resolution.     

Hearing thresholds for pure tones above 16 kHz

Hearing thresholds for pure tones between 16 and 30 kHz were measured by an adaptive method. The maximum presentation level at the entrance of the outer ear was about 110 dB SPL. To prevent the listeners from detecting subharmonic distortions in the lower frequencies, pink noise was presented as a masker. Even at 28 kHz, threshold values were obtained from 3 out of 32 ears. No thresholds were obtained for 30 kHz tone. Between 20 and 28 kHz, the threshold tended to increase rather gradually, whereas it increased abruptly between 16 and 20 kHz.     

Noise-induced temporary threshold shift and recovery in Yangtze finless porpoises Neophocaena phocaenoides asiaeorientalis

In Yangtze finless porpoises Neophocaena phocaenoides asiaeorientalis, the effects of fatiguing noise on hearing thresholds at frequencies of 32, 45, 64, and 128 kHz were investigated. The noise parameters were: 0.5-oct bandwidth, -1 to +0.5 oct relative to the test frequency, 150 dB re 1 μPa (140-160 dB re 1 μPa in one measurement series), with 1-30 min exposure time. Thresholds were evaluated using the evoked-potential technique allowing the tracing of threshold variations with a temporal resolution better than 1 min. The most effective fatiguing noise was centered at 0.5 octave below the test frequency. The temporary threshold shift (TTS) depended on the frequencies of the fatiguing noise and test signal: The lower the frequencies, the bigger the noise effect. The time-to-level trade of the noise effect was incomplete: the change of noise level by 20 dB resulted in a change of TTS level by nearly 20 dB, whereas the tenfold change of noise duration resulted in a TTS increase by 3.8-5.8 dB.     

Kanamycin induced low-frequency hearing loss in the budgerigar (Melopsittacus undulatus)

The chronic effects of kanamycin (KM) on hearing in the budgerigar were investigated by behavioral audiometry. The birds received a daily intramuscular injection of KM (100 mg/kg or 200 mg/kg) for 10 successive days, and absolute thresholds between pre- and post-treatment were compared. KM induced both transient and permanent low-frequency specific hearing loss; i.e., the elevation of threshold for frequencies below 1 kHz was greater than that for frequencies above 1 kHz. Moreover, the degree of hearing loss was dose dependent. The low-frequency selective effects of KM in the present study were contrary to the high-frequency specificity of aminoglycoside ototoxicity in mammals. To assess the effect of KM on auditory frequency resolution, critical ratios were estimated in pathological birds with low-frequency specific hearing loss. There was a linear relation between shift in critical ratio and shift in absolute threshold, suggesting that the increase in the critical ratio is due to a decrease in the efficiency of the detector mechanism rather than a change in the spectral resolving power of the birds.     

Reference threshold sound-pressure levels for the TDH-50 and ER-3A earphones

Reference threshold sound-pressure levels were established for a new insert earphone, the ER-3A tubephone, and for the TDH-50 earphone. In test-retest comparisons, the tubephone produced estimates of auditory threshold as reliable as the thresholds produced by the supraaural earphone. Reference thresholds were developed for the two earphones from data contributed by three laboratories. While the TDH-50 data are in good agreement with the provisional ANSI 6-cc coupler reference levels (ASHA, 1982), the ER-3A data are at variance with the manufacturer's provisional recommendation for 2-cc coupler reference thresholds for frequencies below 1 kHz. The differences are attributed to physiologic noise that masked the lower frequency thresholds.     

Low-frequency and high-frequency cochlear nonlinearity in humans

Low- and high-frequency cochlear nonlinearity was studied by measuring distortion product otoacoustic emission input/output (DPOAE I/O) functions at 0.5 and 4 kHz in 103 normal-hearing subjects. Behavioral thresholds at both f2's were used to set L2 in dB SL for each subject. Primary levels were optimized by determining the L1 resulting in the largest L(dp) for each L2 for each subject and both f2's. DPOAE I/O functions were measured using L2 inputs from -10 dB SL (0.5 kHz) or -20 dB SL (4 kHz) to 65 dB SL (both frequencies). Mean DPOAE I/O functions, averaged across subjects, differed between the two frequencies, even when threshold was taken into account. The slopes of the I/O functions were similar at 0.5 and 4 kHz for high-level inputs, with maximum compression ratios of about 4:1. At both frequencies, the maximum slope near DPOAE threshold was approximately 1, which occurred at lower levels at 4 kHz, compared to 0.5 kHz. These results suggest that there is a wider dynamic range and perhaps greater cochlear-amplifier gain at 4 kHz, compared to 0.5 kHz. Caution is indicated, however, because of uncertainties in the interpretation of slope and because the confounding influence of differences in noise level could not be completely controlled.     

Temporary threshold shifts in humans exposed to octave bands of noise for 16 to 24 hours.

Groups of human subjects were exposed in a diffuse sound field for 16--24 h to an octave-band noise centered at 4, 2, 1, or 0.5 kHz. Sound-pressure levels were varied on different exposure occasions. At specified times during an exposure, the subject was removed from the noise, auditory sensitivity was measured, and the subject was returned to the noise. Temporary threshold shifts (TTS) increased for about 8 h and then reached a plateau or asymptote. The relation between TTS and exposure duration can be described by a simple exponential function with a time constant of 2.1 h. In the frequency region of greatest loss, threshold shifts at asymptote increased about 1.7 dB for every 1 dB increase in the level of the noise above a critical level. Critical levels were empirically estimated to be 74.0 dB SPL at 4 kHz. 78 dB at 2 kHz, and 82 dB at 1 and 0.5 kHz. Except for the noise centered at 4.0 kHz, threshold shifts were maximal about 1/2 octave above the center frequency of the noise. A smaller second maximum was observed also at 7.0 kHz for the noise centered at 2.0 kHz, at 6.0 kHz for the noise centered at 1.0 kHz, and at 5.5 kHz for the noise centered at 0.5 kHz. After termination of the exposure, recovery to within 5 dB of pre-exposure thresholds was achieved within 24 h or less. Recovery can be described by a simple exponential function with a time constant of 7.1 h. The frequency contour defined by critical levels matches almost exactly the frequency contour defined by the E-weighting network.     

Discrimination of interaural temporal disparities by normal-hearing listeners and listeners with high-frequency sensorineural hearing loss

Thresholds of ongoing interaural time difference (ITD) were obtained from normal-hearing and hearing-impaired listeners who had high-frequency, sensorineural hearing loss. Several stimuli (a 500-Hz sinusoid, a narrow-band noise centered at 500 Hz, a sinusoidally amplitude-modulated 4000-Hz tone, and a narrow-band noise centered at 4000 Hz) and two criteria [equal sound-pressure level (Eq SPL) and equal sensation level (Eq SL)] for determining the level of stimuli presented to each listener were employed. The ITD thresholds and slopes of the psychometric functions were elevated for hearing-impaired listeners for the two high-frequency stimuli in comparison to: the listener's own low-frequency thresholds; and data obtained from normal-hearing listeners for stimuli presented with Eq SPL interaurally. The two groups of listeners required similar ITDs to reach threshold when stimuli were presented at Eq SLs to each ear. For low-frequency stimuli, the ITD thresholds of the hearing-impaired listener were generally slightly greater than those obtained from the normal-hearing listeners. Whether these stimuli were presented at either Eq SPL or Eq SL did not differentially affect the ITD thresholds across groups.     

Forward- and simultaneous-masked thresholds in bandlimited maskers in subjects with normal hearing and cochlear hearing loss

Forward- and simultaneous-masked thresholds were measured at 0.5 and 2.0 kHz in bandpass maskers as a function of masker bandwidth and in a broadband masker with the goal of estimating psychophysical suppression. Suppression was operationally defined in two ways: (1) as a change in forward-masked threshold as a function of masker bandwidth, and (2) as a change in effective masker level with increased masker bandwidth, taking into account the nonlinear growth of forward masking. Subjects were younger adults with normal hearing and older adults with cochlear hearing loss. Thresholds decreased as a function of masker bandwidth in forward masking, which was attributed to effects of suppression; thresholds remained constant or increased slightly with increasing masker bandwidth in simultaneous masking. For subjects with normal hearing, slightly larger estimates of suppression were obtained at 2.0 kHz rather than at 0.5 kHz. For hearing-impaired subjects, suppression was reduced in regions of hearing loss. The magnitude of suppression was strongly correlated with the absolute threshold at the signal frequency, but did not vary with thresholds at frequencies remote from the signal. The results suggest that measuring forward-masked thresholds in bandlimited and broadband maskers may be an efficient psychophysical method for estimating suppression.     

Pure tone audiograms and possible aminoglycoside-induced hearing loss in belugas (Delphinapterus leucas)

A behavioral response paradigm was used to measure pure-tone hearing sensitivities in two belugas (Delphinapterus leucas). Tests were conducted over a 20-month period at the Point Defiance Zoo and Aquarium, in Tacoma, WA. Subjects were two males, aged 8-10 and 9-11 during the course of the study. Subjects were born in an oceanarium and had been housed together for all of their lives. Hearing thresholds were measured using a modified up/down staircase procedure and acoustic response paradigm where subjects were trained to produce audible responses to test tones and to remain quiet otherwise. Test frequencies ranged from approximately 2 to 130 kHz. Best sensitivities ranged from approximately 40 to 50 dB re 1 microPa at 50-80 kHz and 30-35 kHz for the two subjects. Although both subjects possessed traditional "U-shaped" mammalian audiograms, one subject exhibited significant high-frequency hearing loss above 37 kHz compared to previously published data for belugas. Hearing loss in this subject was estimated to approach 90 dB for frequencies above 50 kHz. Similar ages, ancestry, and environmental conditions between subjects, but a history of ototoxic drug administration in only one subject, suggest that the observed hearing loss was a result of the aminoglycoside antibiotic amikacin.     

Auditory filter shapes in subjects with unilateral and bilateral cochlear impairments

The shape of the auditory filter was estimated at three center frequencies, 0.5, 1.0, and 2.0 kHz, for five subjects with unilateral cochlear impairments. Additional measurements were made at 1.0 kHz using one subject with a unilateral impairment and six subjects with bilateral impairments. Subjects were chosen who had thresholds in the impaired ears which were relatively flat as a function of frequency and ranged from 15 to 70 dB HL. The filter shapes were estimated by measuring thresholds for sinusoidal signals (frequency f) in the presence of two bands of noise, 0.4 f wide, one above and one below f. The spectrum level of the noise was 50 dB (re: 20 mu Pa) and the noise bands were placed both symmetrically and asymmetrically about the signal frequency. The deviation of the nearer edge of each noise band from f varied from 0.0 to 0.8 f. For the normal ears, the filters were markedly asymmetric for center frequencies of 1.0 and 2.0 kHz, the high-frequency branch being steeper. At 0.5 kHz, the filters were more symmetric. For the impaired ears, the filter shapes varied considerably from one subject to another. For most subjects, the lower branch of the filter was much less steep than normal. The upper branch was often less steep than normal, but a few subjects showed a near normal upper branch. For the subjects with unilateral impairments, the equivalent rectangular bandwidth of the filter was always greater for the impaired ear than for the normal ear at each center frequency. For three subjects at 0.5 kHz and one subject at 1.0 kHz, the filter had too little selectivity for its shape to be determined.     

Distortion product otoacoustic emissions for hearing threshold estimation and differentiation between middle-ear and cochlear disorders in neonates

Our aim in the present study was to apply extrapolated DPOAE I/O-functions [J. Acoust. Soc. Am. 111, 1810-1818 (2002); 113, 3275-3284 (2003)] in neonates in order to investigate their ability to estimate hearing thresholds and to differentiate between middle-ear and cochlear disorders. DPOAEs were measured in neonates after birth (mean age = 3.2 days) and 4 weeks later (follow-up) at 11 test frequencies between f2 = 1.5 and 8 kHz and compared to that found in normal hearing subjects and cochlear hearing loss patients. On average, in a single ear hearing threshold estimation was possible at about 2/3 of the test frequencies. A sufficient test performance of the approach is therefore suggested. Thresholds were higher at the first measurement compared to that found at the follow-up measurement. Since thresholds varied with frequency, transitory middle ear dysfunction due to amniotic fluid instead of cochlear immaturity is suggested to be the cause for the change in thresholds. DPOAE behavior in the neonate ears differed from that found in the cochlear hearing loss ears. From a simple model it was concluded that the difference between the estimated DPOAE threshold and the DPOAE detection threshold is able to differentiate between sound conductive and cochlear hearing loss.     

Effects of periodic rest on physiological measures of auditory sensitivity following exposure to noise

Whole nerve action potential (AP) and single auditory-nerve fiber thresholds were measured in chinchillas exposed to noise. The exposure stimulus was a 500-Hz octave band of noise presented at 95 dB SPL for 15 min/h, for 4 or 40 days. The AP thresholds were elevated by about 40 dB on day 4, between 0.5 kHz and approximately 8 kHz. On day 40, AP thresholds at the same frequencies were lower by 10-25 dB, even though the noise exposure had continued. Single fiber threshold tuning curves exhibited pathologies similar to those previously observed following noise exposure. Tuning curves measured on day 40 were more normal in appearance. These results confirm that similar recovery of threshold observed in psychophysical experiments [Clark et al., J. Acoust. Soc. Am. 82, 1253-1264 (1987)] can be understood in terms of the sensitivity of the peripheral auditory system.     

Temporary shift in masked hearing thresholds of bottlenose dolphins, Tursiops truncatus, and white whales, Delphinapterus leucas, after exposure to intense tones

A behavioral response paradigm was used to measure masked underwater hearing thresholds in five bottlenose dolphins and two white whales before and immediately after exposure to intense 1-s tones at 0.4, 3, 10, 20, and 75 kHz. The resulting levels of fatiguing stimuli necessary to induce 6 dB or larger masked temporary threshold shifts (MTTSs) were generally between 192 and 201 dB re: 1 microPa. The exceptions occurred at 75 kHz, where one dolphin exhibited an MTTS after exposure at 182 dB re: 1 microPa and the other dolphin did not show any shift after exposure to maximum levels of 193 dB re: 1 microPa, and at 0.4 kHz, where no subjects exhibited shifts at levels up to 193 dB re: 1 microPa. The shifts occurred most often at frequencies above the fatiguing stimulus. Dolphins began to exhibit altered behavior at levels of 178-193 dB re: 1 microPa and above; white whales displayed altered behavior at 180-196 dB re: 1 microPa and above. At the conclusion of the study all thresholds were at baseline values. These data confirm that cetaceans are susceptible to temporary threshold shifts (TTS) and that small levels of TTS may be fully recovered.     

The influence of carrier level and frequency on modulation and beat-detection thresholds for sinusoidal carriers

This paper is concerned with modulation and beat detection for sinusoidal carriers. In the first experiment, temporal modulation transfer functions (TMTFs) were measured for carrier frequencies between 1 and 10 kHz. Modulation rates covered the range from 10 Hz to about the rate equaling the critical bandwidth at the carrier frequency. In experiment 2, TMTFs for three carrier frequencies were obtained as a function of the carrier level. In the final experiment, thresholds for the detection of either the lower or the upper modulation sideband (beat detection) were measured for "carrier" frequencies of 5 and 10 kHz, using the same range of modulation rates as in experiment 1. The TMTFs for carrier frequencies of 2 kHz and higher remained flat up to a modulation rate of about 100-130 Hz and had similar values across carrier frequencies. For higher rates, modulation thresholds initially increased and then decreased rapidly, reflecting the subjects' ability to resolve the sidebands spectrally. Detection thresholds generally improved with increasing carrier level, but large variations in the exact level dependence were observed, across subjects as well as across carrier frequencies. For beat rates up to about 70 Hz (at 5 kHz) and 100 Hz (at 10 kHz), beat detection thresholds were the same for the upper and the lower sidebands and were about 6 dB higher than the level per sideband at the modulation-detection threshold. At higher rates the threshold for both sidebands increased, but the increase was larger for the lower sideband. This reflects an asymmetry in masking with more masking towards lower frequencies. Only at rates well beyond the maximum of the TMTF did detection for the lower sideband start to be better than that for the upper sideband. The asymmetry at intermediate frequency separations can be explained by assuming that detection always takes place in filters centered above the stimulus spectrum. The shape of the TMTF and the beat-detection data reflects a limitation in resolving fast amplitude variations, which must occur central to the inner-ear filtering. Its characteristic resembles that of a first-order low-pass filter with a cutoff frequency of about 150 Hz.