微流控液滴软模板制备二氧化钛中空微球

应用微流控液滴技术合成功能材料已发展成为一个新兴领域。本文以夹流结构微流控芯片产生的微液滴作为软模板,以液滴模板界面处发生的水解反应生成二氧化钛球壳,并经后续脱核处理,制备二氧化钛中空微球。采用激光诱导荧光成像、扫描电镜等手段对微球形貌结构进行了分析表征。结果表明,通过控制微流控芯片液滴合成条件,可以得到壁厚约2 μm的二氧化钛中空微球。这种以微流控液滴为模板的合成方法简单灵活,若与其他材料改性方法相结合,有望实现对更多元、更复杂功能微球材料的制备,并进一步拓宽其在光电和催化剂材料领域的应用。     

微流控芯片液滴生成与检测技术研究进展

微流控芯片液滴技术是一种操控微小体积液体的新技术,既可实现高通量微观样本的生成及控制,也可进行独立液滴的操作.分散的微液滴单元可作为理想的微反应器,在生物医药中的药物筛选、材料筛选和高附加值微颗粒材料合成领域展现出巨大的应用潜力.液滴微流控芯片是利用流体剪切力的改变,使互不相溶的两相流体在其界面处生成稳定、有序的液滴,...     

微流控液滴技术:微液滴生成与操控

微液滴技术因具有高通量两相分割分离能力,吸引众多不同领域研究者的关注.本文回顾了微流控液滴技术领域的一些基本技术思路,涉及微液滴的流控生成方法,包括水动力法、电动法、气动法、光控法等,以及液滴生成后的操控技术,如液滴定向位移、融合、裂分、混合、分选、捕获等,同时对这些方法作了简要评述.     

微液滴微流控芯片:微液滴的形成、操纵和应用

微流控芯片中形成的微液滴粒径均一、可控,与传统的连续流体系相比,具有能实现试剂的快速混合、通量更高等优点.本文介绍了微流控芯片中由微通道控制的微液滴的形成、分裂、合并、混合、分选和捕获等微液滴操纵技术,以及微液滴技术在纳米粒子、聚合物微粒的合成、纳米粒子自组装、蛋白质结晶研究和DNA、细胞分析等领域的研究进展.     

基于液滴技术的微流控芯片实验室

液滴微流控系统是微流控芯片领域的一个新的分支,由于其诸多独特的优势而得到了广泛的研究和报道。本文对液滴的制备和相关的操控技术,包括液滴的分裂、融合、混合、分选、存储和编码等进行了介绍,对液滴技术近年来在化学与生物化学分析等领域中的应用进行了综述,并展望了液滴微流控技术的发展前景。     

微流控芯片中微液滴的操控及其应用

微流控芯片中微液滴的操控方法主要有多相流法、电润湿法、热毛细管法、介电电泳法和气动法;应用范围包括乳化、混合、包埋、萃取、微反应器以及生物鉴定等。论文展望了微液滴的发展前景。     

基于微流控技术的蛋白质结晶及其筛选方法的研究进展简

微流控技术以其高通量、低消耗和集成化等优点成为蛋白质结晶微型化研究的重要手段. 本文综述了基于微流控技术的蛋白质结晶技术和方法,主要包括微泵微阀、液滴(Droplet)、滑动芯片(SlipChip)以及液滴实验室(DropLab)等技术. 此外,还针对当前膜蛋白在结构生物学研究中的重要地位,综述了应用于膜蛋白结晶的微流控技术的研究进展.     

基于微流控技术的蛋白质结晶及其筛选方法的研究进展

微流控技术以其高通量、 低消耗和集成化等优点成为蛋白质结晶微型化研究的重要手段. 本文综述了基于微流控技术的蛋白质结晶技术和方法, 主要包括微泵微阀、 液滴(Droplet)、 滑动芯片(SlipChip)以及液滴实验室(DropLab)等技术. 此外, 还针对当前膜蛋白在结构生物学研究中的重要地位, 综述了应用于膜蛋白结晶的微流控技术的研究进展.     

液滴微流控系统在数字聚合酶链式反应中的应用研究进展

数字聚合酶链式反应( PCR)技术近年来发展迅速。与以实时荧光定量PCR为代表的传统PCR技术相比,数字PCR技术显著提高了定量分析的精确度和灵敏度。数字PCR的快速发展与近年来微流控技术在数字PCR技术中的广泛应用有着密切的联系。早期的研究和商业化产品使用的是大规模集成流路微流控芯片,加工过程复杂且价格高昂。近年来,液滴微流控芯片被应用到数字PCR技术中,它可以在短时间内产生102~107个微液滴,每一个微液滴都是最多只含有一个目的基因片段的PCR反应器。 PCR扩增后,通过对单个微液滴的观察计数,就可以获得绝对定量的分析数据。本文综述了不同种类的液滴微流控系统在数字PCR技术中的应用,以及液滴数字PCR微流控芯片在生物、医药、环境等领域的应用。     

微流控液滴技术及其在生物医学分析中的应用进展

微流控芯片微滴技术作为一种基于微流控芯片的操控微小体积液体的新技术,以分散的微滴单元作为微反应器,它大大强化了微流控芯片的高灵敏度、低消耗、高通量和自动化等优点,受到日益广泛的重视,并在物理学、化学和生物学等领域中显示出巨大的应用潜力。文章叙述了微流控微滴领域的最新进展,对新的微滴生成技术及操控技术进行介绍,最后阐述了微滴技术在生物医学分析中的最新研究进展。     

Recent developments of droplets-based microfluidics for bacterial analysis

Droplet-based microfluidics enables the generation of uniform microdroplets at picoliter or nanoliter scale with high frequency(～kHz) under precise control. The droplets can function as bioreactors for versatile chemical/biological study and analysis. Taking advantage of the discrete compartment with a confined volume,(1) isolation and manipulation of a single cell,(2) improvement of in-droplet effective concentrations,(3) elimination of heterogeneous population effects,(4) diminution of contami...     

Surfactants in droplet-based microfluidics

Surfactants are an essential part of the droplet-based microfluidic technology. They are involved in the stabilization of droplet interfaces, in the biocompatibility of the system and in the process of molecular exchange between droplets. The recent progress in the applications of droplet-based microfluidics has been made possible by the development of new molecules and their characterizations. In this review, the role of the surfactant in droplet-based microfluidics is discussed with an emphasis on the new molecules developed specifically to overcome the limitations of 'standard' surfactants. Emulsion properties and interfacial rheology of surfactant-laden layers strongly determine the overall capabilities of the technology. Dynamic properties of droplets, interfaces and emulsions are therefore very important to be characterized, understood and controlled. In this respect, microfluidic systems themselves appear to be very powerful tools for the study of surfactant dynamics at the time- and length-scale relevant to the corresponding microfluidic applications. More generally, microfluidic systems are becoming a new type of experimental platform for the study of the dynamics of interfaces in complex systems.     

Droplet-based microreactor for the production of micro/nano-materials

Droplet-based microreactors are of great interest to researchers due to their incredible ability in the synthesis of micro/nano-materials with multi-function and complex geometry. In recent years, a broad range of micro/nano-materials has been synthesized in droplet-based microreactors, which provide apparent advantages, such as better reproducibility, reliable automation, and accurate manipulation. In this review, we give a comprehensive and in-depth insight into droplet-based microreactors, covering fundamental research from droplet generation and manipulation to the applications of droplet-based microreactors in micro/nano-material generation. We also explore the outlook for droplet-based microreactors and challenges that lie ahead and give a possible effort direction. We hope this review will promote communications among researchers and entrepreneurs.     

Droplet microfluidics for the study of artificial cells

In this review, we describe recent advances in droplet-based microfluidics technology that can be applied in studies of artificial cells. Artificial cells are simplified models of living cells and provide valuable model platforms designed to reveal the functions of biological systems. The study of artificial cells is promoted by microfluidics technologies, which provide control over tiny volumes of solutions during quantitative chemical experiments and other manipulations. Here, we focus on current and future trends in droplet microfluidics and their applications in studies of artificial cells.     

Lab-chip HPLC with integrated droplet-based microfluidics for separation and high frequency compartmentalisation

We demonstrate the integration of a droplet-based microfluidic device with high performance liquid chromatography (HPLC) in a monolithic format. Sequential operations of separation, compartmentalisation and concentration counter were conducted on a monolithic chip. This describes the use of droplet-based microfluidics for the preservation of chromatographic separations, and its potential application as a high frequency fraction collector.     

Double emulsions with controlled morphology by microgel scaffolding

Double emulsions are valuable structures that consist of drops nested inside bigger drops; they can be formed with exquisite control through the use of droplet-based microfluidics, allowing their size, composition, and monodispersity to be tailored. However, only little control can be exerted on the morphology of double emulsions in their equilibrium state, because they are deformable and subject to thermal fluctuations. To introduce such control, we use droplet-based microfluidics to form oil-in-water-in-oil double emulsion drops and arrest their shape by loading them with monodisperse microgel particles. These particles push the inner oil drop to the edge of the aqueous shell drop such that the double emulsions adopt a uniform arrested, anisotropic shape. This approach circumvents the need for ultrafast polymerization or geometric confinement to lock such non-spherical and anisotropic droplet morphologies. To demonstrate the utility of this technique, we apply it to synthesize anisotropic and non-spherical polyacrylate-polyacrylamide microparticles with controlled size and shape.     

Droplet microfluidics: recent developments and future applications

We report recent advances in the field of droplet-based microfluidics. Specifically, we highlight the unique features of such platforms for high-throughput experimentation; describe functional components that afford complex analytical processing and report on applications in synthesis, high-throughput screening, cell biology and synthetic and systems biology. Issues including the integration of high-information content detection methods, long term droplet stability and opportunities for large scale and intelligent biological experimentation are also discussed.     

Applying microdroplets as sensors for label-free detection of chemical reactions

Despite its tremendous high-throughput screening capabilities, widespread applications of droplet-based microfluidics are still limited by the poor availability of appropriate analytical assays. Here we report on a novel sensor method that exploits the osmosis-driven change in droplet size as a quantitative and label-free marker for reactions inside the droplets. We present an analysis of the underlying mechanism and apply the method for monitoring metabolic activity at a single-cell level.     

基于微流控芯片技术的秀丽隐杆线虫研究进展

秀丽隐杆线虫具有体积小、生命周期短、结构简单和高基因保守性等特点,是生命科学研究领域中的一种重要模式生物。微流控芯片的通道尺寸与线虫大小相匹配,并可实现灵活集成的线虫操控,为线虫研究提供了一种全新的平台。在微流控平台上,线虫长期培养、固定、分选、精确刺激传递和单线虫包裹等单元操作已经实现,并被应用于线虫神经生物学、行为学、衰老及发育、药物筛选等研究中。该文着重介绍近几年基于微流控芯片技术的线虫研究最新进展,并对其应用前景予以展望。     

Analytical detection techniques for droplet microfluidics—A review

In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has been proved to be a promising platform for performing chemical and biological experiments with ultra-small volumes (picoliter to nanoliter) and ultra-high throughput. The ability to analyze the content in droplet qualitatively and quantitatively is playing an increasing role in the development and application of droplet-based microfluidic systems. In this review, we summarized the analytical detection techniques used in droplet systems and discussed the advantage and disadvantage of each technique through its application. The analytical techniques mentioned in this paper include bright-field microscopy, fluorescence microscopy, laser induced fluorescence, Raman spectroscopy, electrochemistry, capillary electrophoresis, mass spectrometry, nuclear magnetic resonance spectroscopy, absorption detection, chemiluminescence, and sample pretreatment techniques. The importance of analytical detection techniques in enabling new applications is highlighted. We also discuss the future development direction of analytical detection techniques for droplet-based microfluidic systems.