A new triterpenoid compound from stems and leaves of American ginseng

A new dammarane-type triterpenoid compound was isolated from stems and leaves of American ginseng. The structure of the new sapogenin was elucidated by the combined analysis of NMR and HR-ESI-MS as dammar-20S, 25S-epoxy-3β, 12β, 26-triol (1). Compound 1 showed cytotoxic effect on human SM7721 and human Hela cells in vitro.     

A new triterpenoid saponin from Bacopa monniera

A new triterpenoid saponin,bacopaside Ⅸ,was isolated from the whole plant of Bacopa monniera (L.) Wettst.and its structure was elucidated as 3-0-{β-d-glucopyranosyl(1 → 4)[α-1-arabinofuranosyl-(1 → 2)]-β-d-glucop-yranosyl}-20-O-α-1-axabinopyranosyljujubogenin by spectroscopic methods and some chemical transformations.     

A new triterpenoid saponin from Anemone raddeana

A new triterpenoid bisglycoside, named raddeanoside R19, was isolated from the roots of Anemone raddeana. Its structure was elucidated on the basis of ESI-MS, ^13C NMR, and chemical methods.     

A new triterpenoid saponin from Gynostemma pentaphyllum

A new dammarane-type triterpene saponin was isolated from the aerial parts of Gynostemma pentaphyllum (Thunb.) Makino. Its structural elucidation was accomplished mainly on the basis of the interpretation of spectroscopic data, such as IR, HR-TOF-MS and NMR. Its cytotoxic activity was evaluated against one human cancer cell line HL-60 using MTT assay.     

A new triterpenoid saponin from Clematis ganpiniana

A new triterpenoid saponin,named clematiganoside A(1),was isolated from the whole plant of Clematis ganpiniana.Its structure was elucidated on the basis of 1D,2D NMR,TOF-MS and ESI-MS techniques,and physicochemical properties.     

A new bidesmoside triterpenoid saponin from Stauntonia chinensis

A new bidesmoside triterpenoid saponin,named stauntoside C1(1) has been isolated from Stauntonia chinensis.Its structure was established by means of spectral and chemical methods.     

A new triterpenoid saponin from Abrus precatorius Linn

A new triterpenoid saponin, 3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl subprogenin D (1), together with six known triterpenoids: subprogenin D (2), abrusgenic acid (3), triptotriterpenic acid B (4), abruslactone A (5), abrusogenin (6) and abrusoside C (7) were isolated from the leaves and stems of Abrus precatorius. Their structures were elucidated on the basis of physical and NMR analysis, respectively. Compounds 5 and 6 showed moderate cytotoxicity against MCF-7, SW1990, Hela, and Du-145 cell lines. Compounds 1, 2 and 4 were isolated from this plant for the first time.     

One new triterpenoid saponin from Lysimachia davurica

One new saponin named davuricoside N (1) was isolated from the whole plants of Lysimachia davurica, the structure was determined by 1D and 2D NMR, ESI-MS techniques, and chemical methods.     

A new triterpenoid saponin from the roots of Silene viscidula

A new triterpenoid saponin, silenoviscoside F (1), was isolated from the roots of Silene viscidula, together with three known saponins, dianchinenoside D (2), sinocrassuloside I (3) and sinocrassuloside II (4). The structure of compound 1 was elucidated by spectroscopic data, GC-MS and chemical methods. The structures of compounds 2-4 were determined on the basis of spectroscopic data comparison with the literature values, which were isolated from the genus Silene for the first time.     

A new triterpenoid saponin from the roots of Phytolacca acinosa

A new triterpenoid saponin, named esculentoside T (1), together with five known compounds (2-5), was isolated from the roots of Phytolacca acinosa. The structures of these compounds were elucidated on the basis of spectral data.     

A new triterpenoid saponin from the roots of Ardisia crenata

A new triterpenoid saponin.3 β-O-{ β-D-glucopyranosyl-(1—4)-[ β-D-glucopyranosyl-[1-2)]- α-L-arabinopyranosyl}-16α, 28-dihydroxyolean-12-en-30-oic acid 30-O- β-D-glucopyranosyl ester(ardisicrenoside N.1),together with two known saponins, ardisicrenoside C(2) and D(3),were isolated from the roots of Ardisia crenata Sim.Their structures were elucidated by extensive spectral analysis and chemical evidences.Saponins 1 showed cytotoxicity against MCI-7 and NCI-H460 cancer cell lines at 11.0μmol/L and 22.1μmol/L in vitro.     

A new triterpenoid saponin from the roots of Platycodon grandiflorum

A new triterpenoid saponin, 3-O-β-D-glucopyranosyl 16-oxo-platycodigenin 28-O-β-D-apiofuranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyra-nosyl ester, was isolated from the roots of Platycodon grandiflorum, together with three known saponins, including platycodin D, deapio platycoside E and platycoside E. The structure of the new compound, named 16-oxo-platycodin D, was elucidated on the basis of spectroscopic data.     

A new triterpenoid saponin from Gleditsia sinensis and its antiproliferative activity

Chemical investigation of the anomalous fruits of Gleditsia sinensis led to the isolation and identification of a new triterpenoid saponin, 3-O-β-D-xylopyranosyl-(1 → 2)-α-L-arabinopyranosyl-(1 → 6)-β-D-glucopyranosyl oleanolic acid 28-O-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyrano--syl-(1 → 4)-β-D-xylopyranosyl-(1 → 4)-α-L-rhamnopyranosyl-(1 → 3)-β-D-glucopyranosyl ester (1), along with other nine known compounds (2–10). All the isolates from this species were reported for the first time. The structure of Compound 1 was determined by a detailed analysis using various analytical techniques, including 1D and 2D NMR. In vitro antiproliferative activities of Compound 1 on MCF-7 and Hep-G2 tumor cell lines were evaluated. IC₅₀ values against the two cell lines were 9.5 and 11.6 μM, respectively.     

A new triterpenoid saponin from Pulsatilla cernua predicted by NMR-based mosaic method

Abstract

A saponin (1) with gypsogenin as aglycone was isolated from the roots of Pulsatilla cernua. The aglycone of compound 1 was considered as gypsogenin which was rarely found in this genus. Its structure was predicted by NMR-based “mosaic” method rapidly, and further confirmed on the basis of spectroscopic data, including 2D NMR spectra and chemical evidence. This work suggested that NMR-based mosaic method is suitable for most of saponins from common species of genus Pulsatilla.     

A new bioactive triterpenoid saponin from the seeds of Lactuca scariola Linn

A new triterpenoid saponin, 3-O-[beta-D-galactopyranosyl-(1 --> 3)-O-beta-D-xylopyranosyl-(1 --> 4)-O-alpha-L-rhamnopyranosyl]-oleanolic acid; has been isolated from the seeds of Lactuca scariola. Its structure was determined by various spectral analysis and chemical degradations. This compound shows antimicrobial activity against various bacteria and fungi.     

A new biologically active triterpenoid saponin from the leaves of Lepidagathis hyalina Nees

A new biologically active triterpenoid saponin m.f. C42H68O13, m.p. 315 degrees-317 degrees C was isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of Lepidagathis hyalina. Its structure was characterized as 3-beta-O-[alpha-L-rhamnopyranosyl(1-->4)O-beta-D-glucopyranosyl]16-alpha-hydroxy-olean-12-en(13)-28-oic acid by several spectral and chemical analysis. This new triterpenoid saponin showed antimicrobial activity against various plants pathogenic bacteria and fungi.     

A new triterpenoid saponin from Clinopodium chinense (Benth.) O. Kuntze

A new triterpene saponin, 3β,16β,23α,28β,30β-pentahydroxyl-olean-11,13(18)-dien-3β-yl-[β-d-glucopyranosyl-(1→2)]-[β-d-glucopyranosyl-(1→3)]-β-d-fucopyranoside, was named Clinoposaponin D (1), together with six known triterpene saponins, buddlejasaponin IVb (2), buddlejasaponin IVa (3), buddlejasaponin IV (4), clinopodisides D (5), 11α,16β,23,28-Tetrahydroxyolean-12-en-3β-yl-[β-d-glucopyranosyl-(1→2)]-[β-d-glucopyranosyl-(1→3)]-β-d-fucopyranoside (6) and prosaikogenin A (7), and two known triterpenes, saikogenin A (8) and saikogenin F (9) were isolated from Clinopodium chinense (Benth.) O. Kuntze. Their structures were elucidated on the basis of 1D, 2D NMR and MS analysis. Meanwhile, the effects of all compounds on rabbit platelet aggregation and thrombin time (TT) were investigated in vitro. Compounds 4 and 7 had significant promoting effects on platelet aggregation with EC₅₀ value at 53.4 and 12.2 μM, respectively. In addition, the highest concentration (200 μM) of compounds 2 and 9 shortened TT by 20.6 and 25.1%, respectively.     

A new triterpenoid saponin from the leaves and stems of Panax quinquefolium L.

One new triterpenoid saponin,quinquenoside L_(17)(1),was isolated from the leaves and stems of Panax quinquefolium L.,and its structure was elucidated as 20-O-[(β-D-xylopyranosyl-(1-6)-O-β-D-glucopyranosyl)]-6-O-β-D-glucopyranosyl-dammar-24-ene- 3,6,12,20-tetraol,by the combination analysis of one-dimensional NMR and two-dimensional NMR,mass spectrometry,CD spectrum and chemical evidences.     

A new triterpenoid saponin and a diterpene glucoside from the seeds of Orychophragmus violaceus

One new triterpenoid saponin orychoside A (1) and one new diterpene glucoside orychoside B (12), together with eleven known compounds, were isolated from the seeds of Orychophragmus violaceus. The structures of the new compounds were elucidated on the basis of spectroscopic analysis. The known compounds were identified by comparison with data reported in the literature. All compounds were tested for their cytotoxic activities against the HepG2 and A549 cell lines in vitro. The new compound 1 exhibited potent cytotoxicity against the two cell lines with IC₅₀ values of 7.13 and 8.77 μM, respectively. Compounds 2, 5, 8 and 9 were found to possess obvious cytotoxicity.     

A immunosuppressive triterpenoid saponin from the stems of Epigynum griffithianum

Abstract

Chemical investigation of the stems of Epigunum griffithianum led to the isolation and identification of a new triterpenoid saponin (1) and two known compounds (epigynosides A (2) and B (3)). These structures were elucidated by means of spectroscopic analysis (1D and 2D NMR, MS, UV, IR) as well as comparison with the reported data. Compound 1 was evaluated in vitro for the immunosuppressive activities on proliferation of mice splenocyte and displayed significant immunosuppressive activities compared to the positive control (dexamethasone) with the concentration at 25?μM.