Measurement for serum thyroxine-binding globulin (TBG) and its clinical assessment in diagnosis of thyroid states (author's transl)]

Serum levels of thyroxine (T4)-binding globulin (TBG) were determined by a radioimmunoassay using cellulose-linked antibody to TBG. Values obtained in healthy young adults averaged 1.62 +/- 0.25 (SD) mg/100 ml, and no significant difference wwas detected between males and females. The TBG levels remained within the normal limit in hyperthyroidism while they were significantly increased in hypothyroidism. Interestingly enough, TBG levels were significantly elevated in chronic thyroidities with no overt hypothyroidism. In normal pregnancy, TBG was increased slightly in the first trimester, and markedly in the second and third trimesters. In one case of congenital TBG deficiency, no immunoreactive TBG was detected. It was demonstrated, further, that an inverse relationship (r = -0.7593) existed between the TBG level and serum triiodothyronine uptake index, and that a direct relation (r = +0.6557) was present between the TBG level and T4 in sera from normal subjects and pregnancy. Ratios of T4/TBG were markedly increased in hyperthyroidism, and decreased in hypothyroidism, showing no overlap with the normal subjects, whereas they were below the normal limit in half the cases in the second and third trimesters of pregnancy. The radioimmunoassay for TBG was useful in evaluating hypothyroid states, because it could differentiate the increase in T4 associated with elevated TBG from hyperthyroidism.     

A new solid phase enzyme-immunoassay for total thyroxine (T4), triiodothyronine (T3) and thyroxine-binding globulin (TBG)

Conclusions When comparing the values determined by the radioimmunological and enzymeimmunological methods (Table 1), no significant differences were found in the Wilcoxon test for paired samples. Precision, specifity and accuracy of the EIA are comparable to those of the RIA. There are however important advantages of the EIA: 1) its extreme stability of antigen enzyme conjugates and 2) no further need of radiochemicals.

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Ein neuer solid-phase Enzymimmunoassay für Gesamt-Thyroxin (T₄), Triiodthyronin (T₃) und Thyroxin-bindendes Globulin (TBG)

     

Radioimmunoassay of cholylglycine in serum (author's transl)

Serum levels of cholylglycines (CG) were determined by radioimmunoassay and that of total bile acids (TBA) by enzymatic method. In normal subjects, serum levels of CG, TBA and CG/TBA ratio were 0.6 +/- 0.4 micronM, 7 +/- 2 micronM and 0.08 +/- 0.06, respectively. They were increased markedly in acute hepatitis and moderately in chronic hepatitis and cirrhosis. Thus, measurement of serum CG as compared with serum TBA appears to be a sensitive liver test.