胞质分裂力学及其分子生物学机制的研究

胞质分裂通过一系列的形态学变化使母细胞一分为二生成两个子细胞,几乎整个胞质分裂过程都伴随着力学现象.形成刚度或黏弹性互不相同的区域对细胞形态的改变至关重要,特殊肌动蛋白与交联蛋白是导致细胞局部刚度改变的重要因素.因此有必要深入理解细胞如何变形、以及促进细胞变形的材料特性及其分子生物学基础.本文将针对细胞的力学特性、胞质分裂中的力学现象以及分子生物学基础进行讨论.      

Bauschinger and size effects in thin-film plasticity due to defect-energy of geometrical necessary dislocations

The Bauschinger and size effects in the thinfilm plasticity theory arising from the defect-energy of geometrically necessary dislocations (GNDs) are analytically investigated in this paper. Firstly, this defect-energy is deduced based on the elastic interactions of coupling dislocations (or pile-ups) moving on the closed neighboring slip plane. This energy is a quadratic function of the GNDs density, and includes an elastic interaction coefficient and an energetic length scale L. By incorporating it into the work- conjugate strain gradient plasticity theory of Gurtin, an energetic stress associated with this defect energy is obtained, which just plays the role of back stress in the kinematic hardening model. Then this back-stress hardening model is used to investigate the Bauschinger and size effects in the tension problem of single crystal Al films with passivation layers. The tension stress in the film shows a reverse dependence on the film thickness h. By comparing it with discrete-dislocation simulation results, the length scale L is determined, which is just several slip plane spacing, and accords well with our physical interpretation for the defect- energy. The Bauschinger effect after unloading is analyzed by combining this back-stress hardening model with a friction model. The effects of film thickness and pre-strain on the reversed plastic strain after unloading are quantified and qualitatively compared with experiment results.     

The SPH approach to the process of container filling based on non-linear constitutive models

In this work,the transient free surface of container filling with non-linear constitutive equation’s fluids is numerically investigated by the smoothed particle hydrodynamics(SPH) method.Specifically,the filling process of a square container is considered for non-linear polymer fluids based on the Cross model.The validity of the presented SPH is first verified by solving the Newtonian fluid and OldroydB fluid jet.Various phenomena in the filling process are shown,including the jet buckling,jet thinning,splashing or spluttering,steady filling.Moreover,a new phenomenon of vortex whirling is more evidently observed for the Cross model fluid compared with the Newtonian fluid case.     

Morphological Responses of Vascular Endothelial Cells Induced by Local Stretch Transmitted Through Intercellular Junctions

It has been well established that mechanical stimuli including fluid shear stress and cyclic stretch play a key role in endothelial cell (EC) remodeling. However, in contrast to global remodeling to these mechanical stimuli, little is known of how local mechanical forces are transmitted through cells to induce cell remodeling leading to alteration in cell functions. In this study, we demonstrated that EC remodeling can be exerted by local tension generated in a neighboring EC. In this technique, a glass microneedle was used to apply local stretch in an EC in confluent monolayer and the resulting tension is transmitted to a neighboring EC across intercellular junctions. Local stretch induced reorientation and elongation of ECs parallel to the direction of stretch associated with reorganization of stress fibers. In addition, recruitment of Src homology 2-containing tyrosine phosphatase-2, binding to intercellular adhesion molecules platelet-endothelial cellular adhesion molecules-1, was selectively observed at the force-transmitted intercellular junctions after application of local stretch. These findings suggest that intercellular junctions can not only transmit but also sense local forces, and are potentially involved in EC mechanotransduction pathways.     

Application of split Hopkinson tension bar technique to the study of dynamic fracture properties of materials

A novel approach is proposed in determining dynamic fracture toughness(DFT) of high strength steel,using the split Hopkinson tension bar(SHTB) apparatus,combined with a hybrid experimental-numerical method.The center-cracked tension specimen is connected between the bars with a specially designed fixture device.The fracture initiation time is measured by the strain gage method,and dynamic stress intensity factors(DSIF) are obtained with the aid of 3D finite element analysis(FEA).In this approach,the dimensions of the specimen are not restricted by the connection strength or the stress-state equilibrium conditions,and hence plane strain state can be attained conveniently at the crack tip.Through comparison between the obtained results and those in open publication,it is concluded that the experimental data are valid,and the method proposed here is reliable.The validity of the obtained DFT is checked with the ASTM criteria,and fracture surfaces are examined at the end of paper.     

Shear flow induced vibrations of long slender cylinders with a wake oscillator model

A time domain model is presented to study the vibrations of long slender cylinders placed in shear flow. Long slender cylinders such as risers and tension legs are widely used in the field of ocean engineering. They are subjected to vortex-induced vibrations(VIV) when placed within a transverse incident flow. A three dimensional model coupled with wake oscillators is formulated to describe the response of the slender cylinder in cross-flow and in-line directions. The wake oscillators are distributed along the cylinder and the vortex-shedding frequency is derived from the local current velocity. A non-linear fiuid force model is accounted for the coupled effect between cross-flow and in-line vibrations. The comparisons with the published experimental data show that the dynamic features of VIV of long slender cylinder placed in shear flow can be obtained by the proposed model,such as the spanwise average displacement,vibration frequency,dominant mode and the combination of standing and traveling waves. The simulation in a uniform flow is also conducted and the result is compared with the case of nonuniform flow. It is concluded that the flow shear characteristic has significantly changed the cylinder vibration behavior.     

Rheology of molecular liquids and concentrated suspensions by microscopic dynamical simulations

The rheology and the associated changes that arise in sheared molecular and colloidal liquids are investigated by Molecular and Brownian Dynamics Computer Simulation. Significant shear thinning and normal pressure effects occur in all liquids when the shear rate approximately equals an inverse characteristic relaxation time for the material. The shear and bulk moduli, and self-diffusion coefficients increase with shear rate for all liquids and stable dispersions. The importance for rheology of hydrodynamic coupling between macromolecule trajectories at high packing fractions is demonstrated. The infinite frequency moduli depend on the packing fraction to a power which is effectively the same for all materials, i.e. ca. 3.5, above a percolation transition at a packing fraction 0.25. The suspending fluid enhances the degree of shear thinning above that of the corresponding single component fluid consisting of pure macroparticles.     

Stabilization control of a hovering model insect: lateral motion

Our previous study shows that the lateral disturbance motion of a model drone fly does not have inherent stability (passive stability),because of the existence of an unstable divergence mode.But drone flies are observed to fly stably.Constantly active control must be applied to stabilize the flight.In this study,we investigate the lateral stabilization control of the model drone fly.The method of computational fluid dynamics is used to compute the lateral control derivatives and the techniques of eigenvalue and eigenvector analysis and modal decomposition are used for solving the equations of motion.Controllability analysis shows that although inherently unstable,the lateral disturbance motion is controllable.By feeding back the state variables (i.e.lateral translation velocity,yaw rate,roll rate and roll angle,which can be measured by the sensory system of the insect) to produce anti-symmetrical changes in stroke amplitude and/or in angle of attack between the left and right wings,the motion can be stabilized,explaining why the drone flies can fly stably even if the flight is passively unstable.     

A class of exact solutions for the incompressible viscous magnetohydrodynamic flow over a porous rotating disk

The present paper is concerned with a class of exact solutions to the steady Navier-Stokes equations for the incompressible Newtonian viscous electrically conducting fluid flow due to a porous disk rotating with a constant angular speed.The three-dimensional hydromagnetic equations of motion are treated analytically to obtained exact solutions with the inclusion of suction and injection.The well-known thinning/thickening flow field effect of the suction/injection is better understood from the constructed closed form velocity equations.Making use of this solution,analytical formulas for the angular velocity components as well as for the permeable wall shear stresses are derived.Interaction of the resolved flow field with the surrounding temperature is further analyzed via the energy equation.The temperature field is shown to accord with the dissipation and the Joule heating.As a result,exact formulas are obtained for the temperature field which take different forms corresponding to the condition of suction or injection imposed on the wall.     

Deformation of plastically compressible hardening-softening-hardening solids

Motivated by a model of the response of vertically aligned carbon nanotube (VACNT) pillars in uniaxial compression, we consider the deformation of a class of compressible elastic-viscoplastic solids with a hardening-softening-hardening variation of flow strength with plastic strain. In previous work (Hutchens et al. 2011) a constitutive relation was presented and used to model the response of VACNT pillars in axisymmetric compression. Subsequently, it was found that due to a programming error the constitutive relation presented in the paper (Hutchens et al. 2011) was not the one actually implemented. In particular, the plastic flow rule actually used did not satisfy plastic normality. Here, we present the constitutive formulation actually implemented in the previous work (Hutchens et al. 2011). Dynamic, finite deformation, finite element calculations are carried out for uniaxial compression, uniaxial tension and for indentation of a "half-space" by a conical indenter tip. A sequential buckling-like deformation mode is found in com- pression when there is plastic non-normality and hardening-softening-hardening. The same material characterization gives rise to a Lüders band-like deformation mode in ten- sion. When there is a deformation mode with a sharp front along mesh boundaries, the overall stress-strain response contains high frequency oscillations that are a mesh artifact. The responses of non-softening solids are also analyzed and their overall stress-strain behavior and deformationmodes are compared with those of hardening-softening- hardening solids. We find that indentation with a sharp in- denter tip gives a qualitatively equivalent response for hardening and hardening-softening-hardening solids.     

On the energy conservation and critical velocities for the propagation of a ＂steady-shock＂ wave in a bar made of cellular material

The propagation of shock waves in a cellular bar is systematically studied in the framework of continuum solids by adopting two idealized material models, viz. the dynamic rigid, perfectly plastic, locking （D-R-PP-L） model and the dynamic rigid, linear hardening plastic, locking （D-R-LHP-L） model, both considering the effects of strain-rate on the material properties. The shock wave speed relevant to these two models is derived. Consider the case of a bar made of one of such material with initial length L 0 and initial velocity v i impinging onto a rigid target. The variations of the stress, strain, particle velocity, specific internal energy across the shock wave and the cease distance of shock wave are all determined analytically. In particular the ＂energy conservation condition＂ and the ＂kinematic existence condition＂ as proposed by Tan et al. （2005） is re-examined, showing that the ＂energy conservation condition＂ and the consequent ＂critical velocity＂, i.e. the shock can only be generated and sustained in R-PP-L bars when the impact velocity is above this critical velocity, is incorrect. Instead, with elastic deformation, strain-hardening and strain-rate sensitivity of the cellular materials being considered, it is appropriate to redefine a first and a second critical impact velocity for the existence and propagation of shock waves in cellular solids. Starting from the basic relations for shock wave propagating in D-R-LHP-L cellular materials, a new method for inversely determining the dynamic stress-strain curve for cellular materials is proposed. By using e.g. a combination of Taylor bar and Hopkinson pressure bar impact experimental technique, the dynamic stress-strain curve of aluminum foam could bedetermined. Finally, it is demonstrated that this new formulation of shock theory in this one-dimensional stress state can be generalized to shocks in a one-dimensional strain state, i.e. for the case of plate impact on cellular materials, by simply making proper replacements of the elastic and plastic constants.     

Breakup of a capillary bridge of suspensions

The breakup of liquid bridges under the action of capillary forces is used for studying the rheology of suspensions under stretching. The experiments were performed with suspensions of finegrained (3–30 μm) sand in glycerin for sand volume fractions up to 0.465. The bridge thinning process was registered using an electro-optical measuring device and videofilming. The results were analyzed on the basis of a theory developed earlier for the thinning of a liquid bridge under the action of capillary forces. It is found that, for fairly slow stretching realized in the initial stage of the thinning, the rheological behavior of the suspensions considered agrees with the model of a Newtonian viscous fluid. Along with this, the measured effective viscosity of the suspension turned out to be approximately two-fold greater than the suspension viscosity under shear. The origin of this discrepancy is analyzed. With increase in the stretching rate, in the final stage of the thinning, the weakening of the suspension occurs, which is manifested in the formation of a local rapidly thinning neck in the bridge, similar to that observed in the breakup of plastic materials.     

Failure-tolerant control for small agile satellites using single-gimbal control moment gyros and magnetic torquers

This paper focuses on the attitude control problem of small agile satellites using single-gimbal control moment gyros(CMG) and magnetic torquers(MTQ).CMGs are regarded as effective torque generators for agile satellites because of their torque amplification capability.However,they are vulnerable to failure due to their complex inner mechanism.In this paper,different failure cases of CMGs are analyzed.A flexible failure-tolerant control strategy is developed by automatically redistributing the required control torque among the operating CMGs and MTQs,with a variable limiter to accommodate the actuator dynamics changes introduced by CMG failures.The performances of maneuvers about different directions under different failure cases are also discussed and examined.Numerical simulations demonstrate that the proposed strategy maintains certain agility in the cases of one or two CMGs failing.Moreover,a survival strategy with only one CMG left is also verified.Both sun-pointing stabilization and earth-pointing stabilization can be achieved in this case,which fulfill some basic mission requirements.     

Extensional dynamics of viscoplastic filaments: II. Drips and bridges

A model for the dynamics of slender filaments of Herschel–Bulkley fluid is used to explore viscoplastic dripping under gravity and thinning under controlled extension (liquid bridges). The conditions required for fluid to yield are delineated, and the subsequent thinning and progression to pinch-off are tracked numerically. Calculations varying the dimensionless parameters of the problem are presented to illustrate the effect of surface tension, rheology, inertia (for dripping) and gravity. The theoretical solutions are compared with laboratory experiments using aqueous solutions of Carbopol and Kaolin suspensions. For drips and bridges, experiments with Carbopol are well matched by the theory, using a surface tension equal to that of water, even in situations when the fluid is not slender. Experiments with Kaolin do not compare well with theory for physically plausible values of the surface tension. Implications for rheometry and surface-tension inference are discussed.     

Particles accelerate the detachment of viscous liquids

During detachment of a viscous fluid extruded from a nozzle, a filament linking the droplet to the latter is formed. Under the effect of surface tension, the filament thins until pinch-off and final detachment of the droplet. In this paper, we study the effect of the presence of individual particles trapped in the filament on the detachment dynamics using granular suspensions of small volume fractions (??<?6 %). We show that even a single particle strongly modifies the detachment dynamics. The particle perturbs the thinning of the thread, and a large droplet of fluid around the particle is formed. This perturbation leads to an acceleration of the detachment of the droplet compared to the detachment observed for a pure fluid. We quantify this acceleration for single particles of different sizes and link it to similar observations for suspensions of small volume fractions. Our study also gives more insight into particulate effects on detachment of denser suspensions and allows to explain the accelerated detachment close to final pinch-off observed previously (Bonnoit et al. Phys Fluids 24(4):043304, 2012).     

Modeling development on the meso-scale reacting transport phenomena in proton exchange membrane fuel cells

The catalyst layer (CL) of proton exchange mem-brane fuel cell (PEMFC) involves various particles and pores in meso-scale, which has an important effect on the mass, charge (proton and electron) and heat transport coupled with the electrochemical reactions. The coarse-grained molecular dynamics (CG-MD) method is employed as a meso-scale structure reconstruction technique to mimic the self-organization phenomena in the fabrication steps of a CL. The meso-scale structure obtained at the equilibrium state is further analyzed by molecular dynamic (MD) software to provide the necessary microscopic parameters for understanding of multi-scale and-physics processes in CLs. The primary pore size distribution (PSD) and active platinum (Pt) surface areas are also calculated and then compared with the experiments. In addition, we also highlight the implementation method to combine microscopic elementary kinetic reaction schemes with the CG-MD approaches to provide insight into the reactions in CLs. The concepts from CG modeling with particle hydrodynamics (SPH) and the problems on coupling of SPH with finite element modeling (FEM) methods are further outlined and discussed to understand the effects of the meso-scale transport phenomena in fuel cells.     

Particle flow study on strength and meso-mechanism of Brazilian splitting test for jointed rock mass

A discrete element method （DEM） called particle flow code （PFC2D） was used to construct a model for Brazilian disc splitting test in the present study. Based on the experimental results of intact Brazilian disc of rock-like material, a set of micro-parameters in PFC2D that reflected the macro-mechanical behavior of rock-like materials were obtained. And then PFC2D was used to simulate Brazilian splitting test for jointed rock mass specimens and specimen containing a central straight notch. The effect of joint angle and notch angle on the tensile strength and failure mode of jointed rock specimens was detailed analyzed. In order to reveal the meso-mechanical mechanism of crack coalescence, displacement trend lines were applied to analyze the displacement evolution during the crack initiation and propagation. The investigated conclusions can be described as follows. （1） The tensile strength of jointed rock mass disc specimen is dependent to the joint angle. As the joint angle increases, the tensile strength of jointed rock specimen takes on a nonlinear variance. （2） The tensile strength of jointed rock mass disc specimen containing a central straight notch distributes as a function of both joint angle and notch angle. （3） Three major failure modes, i.e., pure tensile failure, shear failure and mixed tension and shear failure mode are observed in jointed rock mass disc specimens under Brazilian test. （4） The notch angle roles on crack initiation and and joint angle play important propagation characteristics of jointed rock mass disc specimen containing a central straight notch under Brazilian test.     

A multi-scale simulation of tungsten film delamination from silicon substrate

To bridge the different spatial scales involved in the process of tungsten (W) film delaminating from silicon (Si) substrate, a multi-scale simulation procedure is proposed via a sequential approach. In the proposed procedure, a bifurcation-based decohesion model, which represents the link between molecular and continuum scales, is first formulated within the framework of continuum mechanics. Molecular dynamics (MD) simulation of a single crystal W block under tension is conducted to investigate the effect of specimen size and loading rate on the material properties. The proposed decohesion model is then calibrated by using MD simulation of a single crystal W block under tension and using available experimental data, with a power scaling law to account for the size effect. A multi-scale model-based simulation of W film delamination from Si substrate is performed by using the proposed procedure within the framework of the material point method. The simulated results provide new insights into the mechanisms of the film delamination process.     

Adhesion behavior of endothelial progenitor cells to endothelial cells in simple shear flow

The adhesion of endothelial progenitor cells(EPCs) on endothelial cells(ECs) is one of the critical physiological processes for the regenesis of vascular vessels and the prevention of serious cardiovascular diseases.Here,the rolling and adhesion behavior of EPCs on ECs was studied numerically.A two-dimensional numerical model was developed based on the immersed boundary method for simulating the rolling and adhesion of cells in a channel flow.The binding force arising from the catch bond of a receptor and ligand pair was modeled with stochastic Monte Carlo method and Hookean spring model.The effect of tumor necrosis factor alpha(TNF-α) on the expression of the number of adhesion molecules in ECs was analyzed experimentally.A flow chamber system with CCD camera was set up to observe the top view of the rolling of EPCs on the substrate cultivated with ECs.Numerical results prove that the adhesion of EPC on ECs is closely related to membrane stiff-ness of the cell and shear rate of the flow.It also suggests that the adhesion force between EPC and EC by P-selectin glycoprotein ligand-1 only is not strong enough to bond the cell onto vessel walls unless contributions of other catch bond are considered.Experimental results demonstrate that TNF-α enhanced the expressions of VCAM,ICAM,P-selectin and E-selectin in ECs,which supports the numerical results that the rolling velocity of EPC on TNF-α treated EC substrate decreases obviously compared with its velocity on the untreated one.It is found that because the adhesion is affected by both the rolling velocity and the deformability of the cell,an optimal stiffness of EPC may exist at a given shear rate of flow for achieving maximum adhesion rates.