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1.
《中国化学快报》2020,31(11):2941-2944
Iron is one of the essential trace elements in the human body. It plays an important role in human biology and pathology. Deregulation of iron levels in cells is associated with disease development. In this work, we synthesized a novel near-infrared intramolecular charge transfer (ICT) based ratiometric fluorescent probe to detect Fe2+, by using naphthalimide and indole moieties as building blocks. Our work showed that the radiometric probe has excellent selectivity, sensitivity and rapid response. Moreover, we could successfully perform real-time monitoring of Fe2+ in HeLa cells and C. elegans. 相似文献
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Coumarin derivative 1 was synthesized as an efficient ratiometric chemodosimeter for the detection of Cu(II) in 99% water/DMSO (v/v) at pH 7.0. Mechanism studies suggested that 1 formed a complex with Cu(II) at 2:1 ratio accompanied by quenching of green fluorescence at 524 nm; when the solution was heated to 50 °C for 30 min, Cu(II)-promoted hydrolysis of coumarin lactone moiety of 1 occurred with bright blue fluorescence at 451 nm emerged. With fluorescence intensity ratio detection at 451 nm and 524 nm, 1 features an excellent sensitivity with the detection limit of 15 nmol L−1 toward Cu(II) and a good selectivity over other metal ions. 相似文献
4.
We report herein a new class of metal ion chemosensors and give the first example of a metal-dependent peptidase chemosensor
for metal ions. The chemosensor contains the basic specific Ni(II)-dependent peptide bond hydrolysis sequence (Gly-Ala-Ser-Arg-His-Trp-Lys-Phe-Lys).
The substrate was labeled with a fluorophore at the N-terminal and a quencher at the C-terminal Lys side chain. Initially,
the MOCAc ((7-methoxycoumarin-4-yl)acetyl-) emission was quenched by the nearby quencher. In the presence of Ni(II), the substrate
was irreversibly cleaved at the cleavage site, leading to a 20-fold increase in fluorescence intensity. The chemosensor combines
the high selectivity of a peptidase (at least greater than tenfold for Ni(II) over other metal ions) with the high sensitivity
of fluorescence detection limit of 50 nM and can be applied for the quantitative detection of Ni(II) over a concentration
range of three orders of magnitude. Given this degree of selectivity and sensitivity, our molecular engineering design may
prove useful in the future development of other peptidase-based probes for different metal ions in toxicological and environmental
monitoring. 相似文献
5.
[reaction: see text] A new fluorescent probe, salicylaldehyde rhodamine B hydrazone (1), was synthesized and displayed selective Cu(II)-amplified absorbance and fluorescence emission above 500 nm in neutral buffered media. Upon the addition of Cu(II), the spirolactam ring of 1 was opened and a 1:1 metal-ligand complex was formed. The detection of Cu(II) by 1 at a lower micromolar level was successful even in buffered water. 相似文献
6.
Recent advances in the development of near-infrared fluorescent metal nanoclusters for bioimaging applications have been thoroughly overviewed. 相似文献
7.
Yang RH Chan WH Lee AW Xia PF Zhang HK Li K 《Journal of the American Chemical Society》2003,125(10):2884-2885
A bicyclic cycloadduct 1 bearing a pyrenyl moiety has been synthesized and investigated as a ratiometric fluorescent sensor for AgI. In an aqueous ethanol solution of 1, the presence of silver ion induces the formation of a 1:2 metal-ligand complex, which exhibits a strong intensity enhancement of the pyrene excimer emission at the expense of the emission of monomeric pyrene. 相似文献
8.
Lina Huang Yu Chen Yuqiang Zhao Yumin Wang Junwei Xiong Junfeng Zhang Xianghua Wu Ying Zhou 《中国化学快报》2021,31(11):2941-2944
Iron is one of the essential trace elements in the human body. It plays an important role in human biology and pathology. Deregulation of iron levels in cells is associated with disease development. In this work, we synthesized a novel near-infrared intramolecular charge transfer (ICT) based ratiometric fluorescent probe to detect Fe2+, by using naphthalimide and indole moieties as building blocks. Our work showed that the radiometric probe has excellent selectivity, sensitivity and rapid response. Moreover, we could successfully perform real-time monitoring of Fe2+ in HeLa cells and C. elegans. 相似文献
9.
Phosgene has attracted wide attention because of its important applications and value in modern industry, agriculture, and other fields, though it easily leaks and is difficult to detect. In this work, we designed and synthesized a naphthalimide-based fluorescent probe, which is easy to prepare, stable, and able to discriminate between phosgene, acetyl chloride, oxalyl chloride, thionyl chloride, phosphorus oxychloride, and tosyl chloride. Our results indicate that the probe can react with phosgene selectively and sensitively, showing remarkable ratiometric fluorescence changes. Furthermore, the probe can be made into test strips, which can determine phosgene in air effectively. The present work provides a novel class of naphthalimide-based derivatives with potential application in phosgene sensing in real time simply and safely with further optimization. 相似文献
10.
《Journal of Coordination Chemistry》2012,65(5):921-928
A new fluorescent derivative (1) containing coumarin exhibits Fe(III)-selective strong yellow-green fluorescence in ethanol. This compound could be used as an “off-on” chemosensor and allow the detection of Fe3+ by monitoring changes in absorption and fluorescence spectra. Upon addition of Fe3+, an overall emission change of 125-fold was observed. High selectivity and sensitivity were observed over other metal ions, mainly due to the spirolactam ring-opening power of Fe3+. The detection limit was as low as 5.6?ppb. Photo-induced electron transfer, coupled with intramolecular charge transfer are proposed to account for the observed spectral response. 相似文献
11.
Yangyang Yang Chen Zhang Rizhao Pan Shiwei Zhang Shengtao Yao Yao Tang Weilong Zhu Liyue Wang Weiping Zhu Yufang Xu Xuhong Qian 《中国化学快报》2020,31(1):125-128
Alkaline phosphatase(ALP)is one of essential biomarkers in mammalian tissue.Here we report a ratiometric probe for ALP,which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide(BTHPB).The enzymatic dephosphorylation converts the probe to BTHPB,which exhibits a large spectral red-shift(120 nm),allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL.The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells. 相似文献
12.
Zhi Li Juan Ou-Yang Ya-Jun Fu Chun-Yan Li Yong-Fei Li Song-Jiao Li 《Tetrahedron letters》2017,58(36):3536-3540
A borondipyrrolemethene-based compound (1) is synthesized and used as a “turn-on” fluorescent probe for silver ions (Ag+). The probe displays highly sensitive fluorescence response toward Ag+ with a 40-fold fluorescence enhancement when 60 μM of Ag+ is added. The fluorescence intensity of the probe is linearly dependent on Ag+ concentration ranging from 0.05 to 60 μM. And the detection limit (LOD) can reach 0.02 μM, which complies with the standard of World Health Organization (WHO) for drinking water (0.9 μM). Moreover, the probe shows remarkable selectivity for Ag+ over other metal ions. Furthermore, the response behavior of 1 toward Ag+ is pH independent in the neutral range from 6.0 to 8.0. The response of 1 toward Ag+ is fast (response time is less than 2 min) and reversible chemically. What’s more, the sensing mechanism of probe 1 toward Ag+ is verified by mass spectra (MS) and density functional theory (DFT) calculations. In particular, the probe is applied for detection of Ag+ in water samples and living cells successfully. 相似文献
13.
Yanyan Zhao Ole Tietz Wei-Li Kuan Abdul K. Haji-Dheere Stephen Thompson Benjamin Vallin Elisabetta Ronchi Gergely Tth David Klenerman Franklin I. Aigbirhio 《Chemical science》2020,11(18):4773
Soluble forms of aggregated tau misfolded protein, generally termed oligomers, are considered to be the most toxic species of the different assembly states that are the pathological components of neurodegenerative disorders. Therefore, a critical biomedical need exists for imaging probes that can identify and quantify them. We have designed and synthesized a novel fluorescent probe, pTP-TFE for which binding and selectivity profiles towards aggregated tau and Aβ proteins were assessed. Our results have shown pTP-TFE to be selective for early forms of soluble tau aggregates, with high affinity of dissociation constants (Kd) = 66 nM, and tenfold selectivity over mature tau fibrils. Furthermore, we found that pTP-TFE is selective for tau over Aβ aggregates and had good cell permeability. This selectivity of pTP-TFE towards early forms of aggregated tau protein ex vivo was also supported with studies on human brain tissue containing tau and Aβ pathology. To the best of our knowledge, this is the first fluorescent molecule to be reported to have this form of selectivity profile, which suggests that pTP-TFE is a unique probe candidate for imaging-based detection of early stages of Alzheimer''s disease and other tauopathies. pTP-TFE imaging probe can distinguish soluble tau aggregated proteins from other aggregated proteins enabling earlier detection of neurodegenerative diseases. 相似文献
14.
Lim CS Han JH Kim CW Kang MY Kang DW Cho BR 《Chemical communications (Cambridge, England)》2011,47(25):7146-7148
We report a two-photon fluorescent probe (ACu1) that can be excited by 750 nm femto-second pulses, shows high photostability and negligible toxicity, and can visualize Cu(+) distribution in live cells and tissues by two-photon microscopy. 相似文献
15.
He H Mortellaro MA Leiner MJ Fraatz RJ Tusa JK 《Journal of the American Chemical Society》2003,125(6):1468-1469
This communication describes a new optical sensor suitable for practical measurement of extracellular (serum or whole blood) potassium. The sensor responds rapidly and reversibly to changes in potassium concentrations typical of whole blood samples. No interferences from clinical concentrations of calcium or pH are observed, and the sodium interference is very minor. Excitation and emission occur in the visible light region. This new potassium sensor is currently used in the Roche OPTI CCA, a commercially available whole blood analyzer. 相似文献
16.
Ma QJ Zhang XB Zhao Y Li CY Han ZX Shen GL Yu RQ 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2009,71(5):1683-1687
N-methyl-alpha,beta,gamma,delta-tetraphenylporphine (NMTPPH) has been used to detect trace amount of zinc ions in ethanol-water solution by fluorescence spectroscopy. The fluorescent probe undergoes a fluorescent emission intensity enhancement upon binding to zinc ions in EtOH/H(2)O (1:1, v/v) solution. The fluorescence enhancement of NMTPPH is attributed to the 1:1 complex formation between NMTPPH and Zn(II) which has been utilized as the basis for the selective detection of Zn(II). The linear response range covers a concentration range of Zn(II) from 5.0x10(-7) to 1.0x10(-5)mol/L and the detection limit is 1.5x10(-7)mol/L. The fluorescent probe exhibits high selectivity over other common metal ions except for Cu(II). 相似文献
17.
Hong Zhang Kun Li Ling-Ling Li Kang-Kang Yu Xin-Yao Liu Meng-Yang Li Nan Wang Yan-Hong Liu Xiao-Qi Yu 《中国化学快报》2019,30(5):1063-1066
A novel near-infrared fluorescent platform with intrinsic lysosome-targeting was reported capable of detecting cysteine in living cells and in vivo. 相似文献
18.
Xu K Sun S Li J Li L Qiang M Tang B 《Chemical communications (Cambridge, England)》2012,48(5):684-686
A near-infrared fluorescent probe (Trp-Cy) for endogenous ozone is presented, which exhibited a large stokes shift about 140 nm and a rapid fluorescence response to ozone with high selectivity and sensitivity. 相似文献
19.
Zhang S Yang C Lu W Huang J Zhu W Li H Xu Y Qian X 《Chemical communications (Cambridge, England)》2011,47(29):8301-8303
The first highly selective and sensitive fluorescent probe Z1 for detection of carbonic anhydrase IX (CA IX) over isoforms CA I and CA II was developed. As demonstrated, Z1 worked effectively in both enzymatic systems and living hypoxia cells. 相似文献
20.
Koide Y Urano Y Hanaoka K Terai T Nagano T 《Journal of the American Chemical Society》2011,133(15):5680-5682
A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to autoxidation and photobleaching, and good tissue penetration of far-red to NIR fluorescence emission. The value of MMSiR was confirmed by real-time imaging of phagocytosis using a fluorescence microscope. wsMMSiR, a more hydrophilic derivative of MMSiR, permitted effective in vivo imaging of HOCl generation in a mouse peritonitis model. This probe is expected to be a useful tool for investigating the wide range of biological functions of HOCl. 相似文献