Summary: Surface‐enhanced Raman scattering (SERS)‐active substrates with high enhancement were prepared by an in situ reduction method. Novel silver/poly(vinyl alcohol) (PVA) nanocomposite films were obtained, in which the silver nitrate, poly(γ‐glutamic acid) (PGA), and PVA acted as precursor, stabilizer, and polyol reducant, respectively. The UV‐visible spectra of the as‐fabricated films showed that the surface plasmon resonance (SPR) absorption band was narrow and of a stronger intensity, which indicates that the Ag nanoparticle size distribution on the substrate was highly uniform. This finding was further confirmed by X‐ray diffraction (XRD), transmission electron microscopy (TEM), and field‐emission scanning electron microscope (FE‐SEM) measurements. It was found that a PGA‐stabilized PVA nanocomposite film revealed the presence of well‐dispersed spherical silver nanoparticles with an average diameter of 90 nm. The new substrate presents high SERS enhancement and the enhanced factor is estimated to be 106 for the detection of benzoic acid.
The Raman scattering enhancement factor for the Raman spectra of benzoic acid on the various nanocomposite films. 相似文献
In pH 6.0-11.2 Britton-Robinson buffer solution, binding of heparin with crystal violet (CV) can result in a significant enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, such as frequency doubling scattering (FDS) and second-order scattering (SOS). Their maximum scattering wavelengths, λex/λem, appear at 492 nm/492 nm for RRS, 984 nm/492 nm for FDS and 492 nm/984 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the three scattering intensities and the concentration of heparin have been investigated. New methods for the determination of trace amounts of heparin based on the RRS, FDS and SOS methods have been developed. The methods exhibit high sensitivities, the detection limit for heparin is 2.9 ng ml−1 for the RRS method, 3.5 ng ml−1 for the FDS method and 3.3 ng ml−1 for the SOS method. The methods have good selectivity and were applied to the determination of heparin in heparin sodium injection samples with satisfactory results. 相似文献
The Raman scattering spectrum of 2,2′-cyanine on colloidal silver metal particles is discussed. Preliminary assignments of some of the vibrational Raman bands to the motions of specific chromophoric units are presented and multiplet character of some bands is discussed. Enhanced Raman scattering of 2,2′-cyanine occurs when the laser radiation is tuned to the J-aggregate absorption feature at 575 nm. The enhancement in Raman intensity is the result of a diminution of fluorescence intensity, as well as a quantitative increase in Raman scattering intensity, and is distinct from other types of enhancement phenomena (e.g., resonance Raman of monomeric solution dye, and surface-enhanced Raman scattering (SERS)). The resonance Raman enhancement, due to excitation at the frequency corresponding to the J-aggregate absorption, is found to be 2 × 10+3. 相似文献
Publications on surface-enhanced Raman scattering (SERS) in metal sols are perused. The discrepancy between extinction spectra for freshly formed sols and the SERS excitation spectra is found to be connected with different temperature modes used in different procedures for obtaining sols, the temperature difference leading to different concentrations of metal adatoms on sol particles. Once the presence of adatoms is accounted for, the nature of “hot” sol particles, which ensure the observation of values of the SERS enhancement coefficient G?1014?1015, can be explained and the reasons for the scarcity of such particles can be established. On compact hot sol particles, rigorous calculations of electromagnetic enhancement Gem in most cases yield Gem≤ 1014. That is why combining the coefficient σb of gigantic amplification of background, which is caused by electron RS in metal and which is connected with the existence of adatoms, with the coefficient σsi of SERS enhancement in a metal-metal adatom-adsorbate adsorption complex gives σbσsi ≥ 108. 相似文献
Surface‐enhanced resonance Raman scattering (SERRS) is not realized for most molecules of interest. Here, we developed a new SERRS platform for the fast and sensitive detection of 2,4,6‐trinitrotoluene (TNT), a molecule with low Raman cross section. A cationic surfactant, cetylpyridinium chloride (CPC) was modified on the surface of silver sols (CP‐capped Ag). CPC not only acts as the surface‐seeking species to trap sulfite‐sulfonated TNT, but also undergoes complexation with it, resulting in the presence of two charge‐transfer bands at 467 and 530 nm, respectively. This chromophore absorbs the visible light that matches with the incident laser and plasmon resonance of Ag sols by the use of a 532.06 nm laser, and offered large resonance Raman enhancement. This SERRS platform evidenced a fast and accurate detection of TNT with a detection limit of 5×10?11 M under a low laser power (200 μW) and a short integration time (3 s). The CP‐capped Ag also provides remarkable sensitivity and reliable repeatability. This study provides a facile and reliable method for TNT detection and a viable idea for the SERS detection of various non‐resonant molecules. 相似文献
The interaction between congo red (CR) and amikacin (AMK) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium, CR combined with AMK to form an ion association complex with the composition ratio of 1∶1 by electrostatic interaction, hydrophobicity and charge transferring effect. As a result, the new spectra of RRS, FDS, and SOS appeared and their intensities were enhanced greatly. The maximum wavelengths of RRS, FDS and SOS were located at 563 nm, 475 nm and 940 nm, and the scattering intensities were proportional to the concentration of AMK. These three methods have very high sensitivities, and the detection limits were 4.0 ng·mL?1 for RRS, 3.6 ng·mL?1 for FDS and 1.9 ng·mL?1 for SOS, respectively. At the same time, the methods have better selectivity. A new method for the determination of trace amounts of AMK with congo red by resonance scattering technique has been developed. The recovery for the determination of AMK in blood serum and urine sample was between 95.5% and 105.5%. In this study, the properties, such as enthalpy of formation, charge distribution and mean polarizability, were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed. 相似文献
Resonance Raman spectra are obtained when the wave number of the exciting radiation is close to, or coincident with, that of an electronic transition of the scattering species. Such spectra are usually characterized by a very large enhancement of the intensities of particular Raman bands, sometimes with the appearance of intense overtone and combination tone progressions. The technique provides detailed information about excited electronic states because it is only the vibrational modes associated with the chromophore that are resonance-Raman active. Additionally, the high sensitivity is such that compounds at concentrations as low as 10?6 mol/L may be detected, enabling resonance Raman spectroscopy to be used as an analytical tool and for the study of chromophores in molecules of biological interest. 相似文献
A novel method for the dynamic determination of cetirizine dihydrochloride in urine using surface enhanced Raman scattering (SERS) has been developed. Comparison of SERS activities between gold and silver colloid was made based on the analysis of the transmission electron micrographs and the SERS spectra, revealing that silver colloid is much more efficient on the signal enhancement performance. The primary sites of the adsorption on the nanoparticle surface were represented by the feature peaks of piperazine at ca. 1050?cm?1 and phenyl rings at ca. 1630?cm?1, respectively. The best signal response was extracted at pH 7 at the Cl?:Ag+ molar ratio of 2:1. The matrix effect was eliminated by subtracting the spectral contribution of the blank urine from the sample spectra. Sample preparation procedures were minimized. Quantification could be simply accomplished on the predicted versus actual concentration curve, within the Raman shift range from 500 to 2500?cm?1. Neither modeling nor complicate calculation was required. The method was shown to be specific and applicable for drug urine concentration monitoring in situ. 相似文献
A micro high performance liquid chromatography coupled with a resonance Raman detection system is described. For highly sensitive Raman detection, aliphatic amines were derivatized with dabsyl chloride (4-dimethylaminoazobenzene-4′-sulfonyl chloride). The derivatives were separated on an ODS micro column (0.5 mm i.d. × 145 mm PTFE tube). Chromatograms were obtained by measuring the intensity of Raman scattering at 1136 cm?1 with the 488.0 nm line of an Arion laser. The lower detection limit was 1.5 ng and the RSD of relative peak height (n = 9) was 5.9% at 11 ng of methylamine derivative. Moreover, by stopping the flow of the micro HPLC system at the retention time of the individual derivatives, it was possible to measure their resonance Raman spectra. 相似文献
Surface enhancement mechanism of Raman scattering from molecules adsorbed on silver oxide colloids is reported. Absorption spectra and Raman spectra of the cyanine dye D266 and pyridine molecules adsorbed on Ag2O colloids, and the influences of S2O32− and OH− on the SERS are studied respectively. The results indicate that ‘chemical' enhancement is dominant in Ag2O colloidal solution. Surface complexes of adsorbed molecules and small silver ion clusters Agn+ as the SERS active sites make an important contribution to surface enhanced Raman scattering (SERS). At these active sites, charge transfer between the adsorbed molecules and the small silver ion clusters is the main enhancement origin. The enhancement factor of D266 adsorbed on Ag2O colloids is theoretically estimated with the excited-state charge transfer model, which is roughly in accordance with the experiments. 相似文献
A surface‐enhanced Raman scattering‐chiral anisotropy (SERS‐ChA) effect is reported that combines chiral discrimination and surface Raman scattering enhancement on chiral nanostructured Au films (CNAFs) equipped in the normal Raman scattering Spectrometer. The CNAFs provided remarkably higher enhancement factors of Raman scattering (EFs) for particular enantiomers, and the SERS intensity was proportional to the enantiomeric excesses (ee) values. Except for molecules with mesomeric species, all of the tested enantiomers exhibited high SERS‐ChA asymmetry factors (g), ranging between 1.34 and 1.99 regardless of polarities, sizes, chromophores, concentrations and ee. The effect might be attributed to selective resonance coupling between the induced electric and magnetic dipoles associated with enantiomers and chiral plasmonic modes of CNAFs. 相似文献
A high quality anodic aluminum oxide (AAO) template with ordered apertures about 50-80 nm was fabricated by anodizing aluminum in electrolytes through a two-step method, and silver nanowires with diameters from 40nm to 70nm were prepared on this AAO template by magnetron sputtering. On the glass covered with silver nanowires, high quality surface enhanced Raman scattering (SERS) spectra of sudan II (C18H16N2O) with enhancement factors of 105 were obtained. And comparison of SERS spectra on silver nanowires with the SERS spectra of silver colloids indicates that main enhanced mode is lightning rod effect of nanorods on the Sudan II/silver nanowires system. 相似文献
A surface-enhanced Raman scattering-chiral anisotropy (SERS-ChA) effect is reported that combines chiral discrimination and surface Raman scattering enhancement on chiral nanostructured Au films (CNAFs) equipped in the normal Raman scattering Spectrometer. The CNAFs provided remarkably higher enhancement factors of Raman scattering (EFs) for particular enantiomers, and the SERS intensity was proportional to the enantiomeric excesses (ee) values. Except for molecules with mesomeric species, all of the tested enantiomers exhibited high SERS-ChA asymmetry factors (g), ranging between 1.34 and 1.99 regardless of polarities, sizes, chromophores, concentrations and ee. The effect might be attributed to selective resonance coupling between the induced electric and magnetic dipoles associated with enantiomers and chiral plasmonic modes of CNAFs. 相似文献
Surface-enhanced Raman scattering (SERS) spectroscopy and density functional theory (DFT) calculations were used to investigate the nature of the charge-transfer (CT) process between nitrothiophenol (NTP) isomers and the n-type semiconductor, TiO2. The Raman signals of p-NTP and m-NTP that were chemisorbed onto TiO2 were significantly enhanced with respect to their corresponding neat compounds. In particular, an enhancement factor (EF) of 102–103 was observed for both p-NTP and m-NTP, with m-NTP displaying a larger EF compared to p-NTP. The Raman signal of o-NTP on TiO2 was not detectable, owing to interference from fluorescence emissions. A molecule-to-TiO2 charge-transfer mechanism was responsible for the enhanced Raman signals observed in p-NTP and m-NTP. This transfer was due to a strong coupling between the adsorbate and the metal oxide, which led to an optically driven CT transition from the HOMO of NTP into the conduction band of TiO2. Based on the mesomeric effect, the NO2 group para to the thiol had a stronger electron-withdrawing ability than the NO2 group at the meta position. A less-efficient CT transition from p-NTP to TiO2 in the surface complex resulted in a weaker Raman-signal enhancement for p-NTP compared to m-NTP. The DFT calculation determined that the HOMO and the LUMO of NTP bound to TiO2 were located entirely on the adsorbate and the semiconductor, respectively, thereby supporting the experimental findings that a molecule-to-TiO2 mechanism was the driving force behind the observed SERS effect. 相似文献
A facile synthesis method for NDs-Au@AgNPs SERS substrate using Au seeds prepared by nitrogen-rich quantum dots (NDs) as reducing agent and stabilizer was developed for nitroaniline isomers recognition by surface-enhanced Raman scattering. 相似文献