声光可调滤光片表面等离子体子共振传感器的研究

自行设计并组装了一套以声光可调滤光片(AOTF)为波长选择系统的波长检测型表面等离子体子共振(SPR)传感器装置,介绍了AOTF的特点和性能.研究了传感器对乙醇、葡萄糖和蔗糖的响应特性,三者的线性范围分别为5%～60%(体积分数),0.028～0.280和0.014～0.140mol/L.并对葡萄糖和蔗糖的混合溶液进行了分析.实际样品的测定结果与国家标准方法的测定值相符合     

用表面等离子体子共振免疫传感器检测B因子

研制了一种新型的基于表面等离子体子共振的光学传感器用于抗原识别。该传感器采用固定入射角,以波长为变量,在400-800nm范围内进行实时监测。分子自组装技术用于在金基底层上形成敏感膜,并对敏感膜形成的动力学过程进行了研究。B因子作为补体C3激活子与多种疾病有关,B因子的检测范围为1-80μgmL。在优化的实验条件下,该传感器具有良好的再现性、再生性及选择性。     

基于表面等离子体子共振的B因子传感器

以分辨率较高的一米光栅单色仪为分光系统,光电倍增管为检测系统,改进了自行组装的表面等离子体子共振(SPR)传感装置,提高了仪器的检测能力约50倍,对于发展改变波长模式的SPR传感器具有重要意义。以对金和抗体均有较强吸附作用的葡萄球菌A蛋白为基底膜,观测了人的B因子抗体和抗原之间免疫反应的动力学过程,并研究了B因子的定量测定。结果表明,B因子抗原的浓度在0.02～5μg/mL范围内与信号的响应值呈线性关系。该传感器灵敏度高,选择性和重现性均好。     

自组装表面等离子体子共振传感器用于测定抗生素-蛋白结合率的研究

自行设计组装了一套以白色发光二极管为光源的新型表面等离子体子共振传感器, 并采用此传感器通过测量动力学参数测定了8种抗生素与固定在金膜上的牛血清白蛋白之间的结合率, 将所得数据与常用的平衡透析法进行了比较, 两者具有良好的可比性和线性相关性, 说明采用此法也可测定抗生素与蛋白的结合率. 该方法具有测定周期短、 样品用量少、 操作简便、 预测效果良好以及仪器易于小型化甚至微型化等优点.     

用表面等离子体子共振传感器测定人心肌肌钙蛋白I的研究

采用自组装表面等离子体子共振(SPR)传感装置,固定入射角,以波长为变量,以CCD为检测系统,用对金和抗体均有较强吸附作用的葡萄球菌A蛋白作为基底膜,观测了人心肌肌钙蛋白I的抗体和抗原之间免疫反应的动力学过程,并进行了人心肌肌钙蛋白I的定量测定.结果表明,人心肌肌钙蛋白I的浓度在5.0～50μg/L范围内与传感器的响应值呈线性关系.     

基于表面等离子体子共振的白蛋白免疫传感器的研究

采用自行组装的全波长表面等离子体子共振(SPR)传感装置,以对金和蛋白抗体均有较强吸附作用的A蛋白作为基底膜,测试了A蛋白在金膜表面结合的动力学常数,其结果为2.3×10⁵L/(mol·s).研究了在A蛋白基底膜上白蛋白抗体的定向自组装程度和速率.观测了白蛋白抗体与抗原之间免疫反应的动力学过程,并优化了实验条件.结果表明,白蛋白抗原的浓度在0.02～10mg/mL范围内与信号的响应值呈良好的线性关系.将此SPR传感器用于糖尿病肾病患者尿蛋白的检测,结果较好.     

γ-干扰素DNA传感器组装过程的表面等离子体子共振研究

自行设计并组装了一套简便实用的多波长表面等离子体子共振DNA传感装置,用于γ-干扰素DNA的检测。以人工合成γ-干扰素(interferongamma,IFN-γ)寡聚核苷酸片段作为DNA探针,用化学法标记生物素探针,利用生物素-亲和素系统相互作用在传感器表面固定DNA探针,使用该SPR传感装置实时监测了DNA探针的固定过程及DNA杂交反应的进行。用于IFN-γ寡聚核苷酸的检测,测定范围为50-400ng/mL;用于IFN-γ的聚合酶链反应(polymerasechainreaction,PCR)扩增产物的检测,其测定范围为5-40ng/mL。同时研究了DNA传感器的稳定性、可逆性及干扰情况。实验结果表明,该传感器可成功地用于检测目的DNA。     

表面等离子体子共振传感器研究头孢菌素类药物与白蛋白的相互作用

简单介绍了自行设计并组装的波长扫描型声光可调滤光片表面等离子体子共振(AOTF-SPR)传感器装置。应用此SPR传感器研究了两种头孢菌素类药物与人血清白蛋白的相互作用。采用胺基偶联的方法将HSA固定在传感器表面。通过AOTF快速扫描,记录共振电压的位移,并通过计算得到头孢克洛与人血清白蛋白相互作用的结合常数为1.17×103L/mol,结合百分率为44.22%;头孢曲松与人血清白蛋白相互作用的结合常数为2.55×102L/mol,结合百分率为14.79%。结果表明:AOTF-SPR传感器装置可用于研究药物与人血清白蛋白的相互作用。     

表面等离子体共振生物传感器及其应用

表面等离子体共振(Surface Plasmon Resonance,SPR)生物传感器在检测生物分子特异性结合方面具有的高灵敏度、免标记及检测快速等优点,使其在过去的20年中在生命科学、医药科学、食品安全等领域取得了快速发展。本文对SPR生物传感器进行了简要介绍,并着重对其在生命科学、医药学、环境分析、食品安全等领域的应用进行了综述,最后对该领域的研究前景进行了展望。     

表面等离子体激元共振光化学传感器的研究

自行设计并组装了一套全波长表面等离子体激元共振光化学传感器实验装置;研究了以银膜为基底的传感器的稳定性、可逆性及干扰情况;在575nm波长处,测定乙醇的灵敏度为4.6×10^-4折射率单位,RSD≤1.4%,定量测定范围为0.5%～70%.分析了一些实际样品,结果与标准方法的测定值相吻合.     

Detection of Penicillin via Surface Plasmon Resonance Biosensor

A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the decomposition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin.     

表面等离子体共振法检测人血清白蛋白抗体活性

表面等离子体共振法是研究生物大分子间相互作用的有效方法之一,和非直接方法（如酶联免疫）相比,具有实时、快速和免标记等特点。我们在甲羧基化葡聚糖修饰的传感片表面直接交联固一人血清白蛋白（ＨＳＡ）,用于ａｎｔｉ－ＨＳＡ抗体活性的检测,并用Ｈ３ＰＯ４（０．１ｍｏｌ／Ｌ）溶液再生。结果表明表面等离子体共振（ＳＰＲ）生物传感器能快速实时检测ａｎｔｉ－ＨＳＡ抗体的活性,且传感片能够重复使用１００次以上。     

表面等离子体共振生物传感器连续检测莱克多巴胺

利用表面等离子体共振生物传感器对莱克多巴胺抗体与固定在芯片表面的莱克多巴胺衍生物的相互作用进行了分析,解离常数为2.56×10-6s-1。根据一定范围内相对响应值和时间近似呈线性关系的动力学特性,建立了连续检测的方法,从而简化了实验步骤,有利于提高芯片的使用寿命。检测莱克多巴胺采用抑制法,将莱克多巴胺衍生物固定在芯片的表面,莱克多巴胺抗体与样品混合后流过芯片的表面,所得相对响应值与样品中莱克多巴胺的浓度成反比。单个样品的检测时间设定为15min,对应的检出限小于4μg/L。     

表面等离子体共振生物传感器研究人参皂甙与血清白蛋白的相互作用

To use a newly developed wavelength modulation surface plasmon resonance （SPR） biosensor, an experimental protocol was developed to investigate the interaction of ginsenosides with serum albumin. With a known concentration of the ginsenosides, bound percentages of the ginsenosides with human serum albumin （HSA） or bovine serum albumin （BSA） were obtained. SPR technique could require no labeling and this method provided the detailed information on association and disassociation of molecules in real time. The results indicate that the sensitivity of wavelength modulation SPR biosensor is sufficient for detection and characterization of binding events involving low-molecular weight compounds and their immobilized protein targets.     

Comparison of Electrochemical and Surface Plasmon Resonance Immunosensor Responses on Single Thin Film

This paper reports results obtained when comparing an electrochemical enzyme immunosensor and a surface plasmon resonance (SPR) based immunosensor on the same gold surface installed in an electrochemical SPR flow cell. Simultaneous electrochemical and SPR measurements were performed on a gold surface modified with multilayers of poly‐L ‐lysine and poly‐styrenesulfonate assembled with the layer‐by‐layer method. First, we obtained the SPR response induced by the formation of an immunocomplex from the shift in the SPR angle by injecting an anti tumor necrosis factor‐α antibody solution labeled with alkaline phosphatase into the flow cell containing the multilayer modified with tumor necrosis factor‐α. Then we compared this SPR result with that obtained for the electrochemical oxidation current of p‐aminophenol catalyzed by alkaline phosphatase from p‐aminophenolphosphate on the same gold film. We compared the two immunosensor responses obtained using the different measurement principles and found that there was a high correlation efficient of 0.973 between them. This was because we were able to immobilize the immunoreagents with good stability and without losing the transport of the enzyme product in the multilayer whose thickness we easily controlled with nanometer scale accuracy. We also report that the detection limit of our electrochemical immunosensor after optimization was around 100 pg/mL (0.4 pM), which is one of the lowest values yet reported for an electrochemical immunosensor.     

Continuous Immunoassay for Sulfamethazine by Surface Plasmon Resonance-Based Biosensor

Regeneration of the sensor chip surface is difficult in many surface plasmon resonance (SPR) biosensor assays. Improper regeneration will reduce life span of the sensor chip and decrease the quality of the data. Considering the advantages of reducing the regeneration frequency, a theoretically feasible continuous SPR biosensor immunoassay for sulfamethazine (SMT) was developed. In the continuous inhibitive immunoassay, the sensor chip surface is regenerated only once after a definite number of tests instead of every test. The SMT-bovine serum albumin (BSA) conjugate was covalently immobilized to a carboxymethyldextran modified gold film. The immobilization conditions of the antigen were studied and the working dilution of the antibody was optimized. The antibody was mixed with SMT of different concentrations prepared with PBS buffer to construct the calibration curve. The limit of detection was 0.5 ng mL^?1. The continuous SPR biosensor assay was proved to be simpler and more practical than a normal one.     

生物分子相互作用动力学的表面等离子体共振研究方法

表面等离子体共振及其成像方法以其免标记、可实时动态原位跟踪反应过程而在生物分子相互作用动力学及其常数测定研究中凸显优势,近年来这方面的研究进展迅速,但缺乏必要的总结归纳.为此,本文就这一主题所涉及的关键研究方法与应用进展作简要的归纳分析并展望了该领域的发展前景.     

Determination of Lysozyme by Thiol-Terminated Aptamer-Based Surface Plasmon Resonance

A sensitive and selective surface plasmon resonance (SPR) aptasensor has been developed for the real-time determination of lysozyme. The thiol-terminated lysozyme aptamer was covalently attached to the surface of sensor through Au–S bonding. In the presence of lysozyme, the aptamer captured lysozyme on the surface and enhanced the SPR signal that was proportional to the concentration of lysozyme. Under the optimized conditions, the SPR signal was linear with lysozyme concentration from 1 to 100?µmol/L. The detection limit for lysozyme was 0.5?µmol/L. The aptasensor also provided high specificity for lysozyme and was unaffected by other proteins. This aptasensor provides rapid, simple, and sensitive determination of lysozyme detection and a promising strategy for other proteins.