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亚甲基蓝共振光谱散射法测定脱氧核糖核酸 总被引:13,自引:0,他引:13
基于脱氧核糖核酸(DNA)对有机染料亚甲基蓝的共振光散射的增强效应,拟定了一种测定了DNA的共振光散射法。在PH5.5-7.5范围内,亚甲基蓝在350nm处的共振光散射增强有度呈线性关系,线性范围为200-1400μg/L,检出限可达15μg/L。该方法简便,快速,用于合成样品中DNA的测定,结果令人满意。 相似文献
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以结晶紫与亚硝基R盐吸光光度法测定镍 总被引:2,自引:2,他引:2
在氨性(PH9-9.5)条件下,镍(Ⅱ)与结晶紫及亚硝基R盐反应形成紫色络合物,表观摩尔吸光系数ε536=2.29×10^5L,mol^-1,镍浓度在0.0120mg/L范围内遵守比耳定律,组成摩尔比为Ni:CV:R=1:2:1,本法快速,为光度测定微量镍的高灵敏方法之一,用于土壤、人发等试样中镍的测定、结果满意。 相似文献
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共振瑞利散射法测定硫氰酸盐—碱性三苯甲烷染料体系中?… 总被引:3,自引:0,他引:3
研究了钼(V)与硫氰酸盐和结晶紫,乙基紫,孔雀石绿,亮绿及碘绿等5种碱性三苯甲烷染料形成离子缔合配合物的共振瑞利散射光谱,考察了它们的光谱特征,影响因素和适宜的反应条件,确定了共振瑞利散射强度与钼(V)浓度之间的关系,方法灵敏度高,不同体系对钼的检出限在2.1~12.0μg/L之间,提出了用共振瑞利散射测定钼的新分析方法,将其用于钢铁中痕量钼的测定获得满意的结果。 相似文献
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镓—茜素紫极谱络合吸附波的研究 总被引:3,自引:0,他引:3
在含有0.05mol/L HAc-NaAc(pH5.6)、0.1mol/L KCL和1.5×10^-5mol/L茜素紫的溶液中,镓(Ⅲ)-茜素紫络合物在单扫示波极谱仪上产生一灵敏的导数极谱波,峰电位在-1.11V(vs.SCE),峰电流与镓(Ⅲ)浓度在2.0×10^-8mol/L。机理研究表明,峰电流是由吸附在电极表面的络合物中的中心离子和配位体同时还原而产生的。方法已应用于铝合金中镓的测定,结果 相似文献
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优化在1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺(EDC)存在下,5′-NH2单末端和5′-TR/3′-NH2双末端修饰单链脱氧核糖核酸(DNA)探针在10μm羧基聚苯乙烯小珠表面进行微阵列的条件,研究5′-NH2单末端修饰DNA探针与其碱基互补充阢进行的固-液杂交特性。结果表明,在PH4.6-5.6,EDC浓度为0.5-0.7g/L时,DNA探针在小珠表面具有最大的微阵列能力。微阵列在小珠表面的5′-HN2单末端修饰DNA探针,与期 碱基互补序列的杂交反应遵守二级反应动力学,杂交优化条件除取决于溶液介质的PH值,离子强度和碱基互补序列 溶液介质中的浓度外,还取决于环境温度和杂交时间。测定了25℃下微阵列在小珠表面的20-merDNA探针与其溶液中互补序列的杂交反应常数。 相似文献
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核酸对氯化银胶体溶液共振光散射的猝灭作用及其应用 总被引:7,自引:0,他引:7
报道了一种测定水溶液中核酸的方法,该法基于核酸对氯化银溶胶共振射光的猝灭作用。在理想测定条件下,散射光的猝灭程度正比于核酸的浓度,三种核酸(calf thymus DNA,herring DNA and YeastRNA)的线性范围分别为0-20μg/L,0-60μg/L和0-80μg/L,检测限分别为0.65μg/L,1.1μg/L和1.9μg/L。6种合成样品的测定结果令人满意,机理研究结果表明,核酸中的碱基(尤其是嘌呤碱)同银离子具有很强的结合能力,这种结合影响了氯化银的沉淀平衡,导致了氯化银溶胶共振散射光的猝灭。 相似文献
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流动注射—结晶紫—磷钼杂多酸离子缔合体系光度法测定水中痕?… 总被引:2,自引:0,他引:2
在一定的酸度条件下,结晶紫与磷钼杂多酸离子缔合物的流动注射分光光度法可直接测定痕量磷。灵敏度高,重现性和选择性好。水中常见离子不干扰痕量磷的测定,工作曲线的线性范围为0 ̄500μg·L^-1的磷,检出下限达1.0μg·L^-1,以120h^-1采样频率,对各种天然水样进行测定,回收率为92.5% ̄105.0%,相对标准偏差为0.1% ̄6.7%。 相似文献
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研究了脱氧核糖核酸的极谱伏安行为,在0.1mol.L^-1NH4Cl溶液中DNA产生一良好的极谱峰,峰电位Ep=-1.65V(vs.Ag/AgCl),且峰电流ip与DNA的浓度在4.5*10^-5-3.7*10^-4mol.LO^-1范围内呈线性关系,可望用于DNA的定量分析。 相似文献
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A sensitive and selective method has been introduced for the determination of ultra trace amounts of Ru(III) based on its catalytic effects on the oxidation of Brilliant green (BG) by sodium bromate. A flow injection method has been used with spectrophotometric detection. The method is based on measuring the decrease in absorbance of BG at lambda(max)=615 nm. The decrease in absorbance of BG is linear with the concentration range of 0.005-0.500 mug ml(-1) Ru(III). The limit of detection is 0.002 mug ml(-1). The influence of reagent concentration, manifold parameter and potential interference species has been investigated. The method was used for the determination of ruthenium in synthetic samples with satisfactory results. 相似文献
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根据头孢克洛在碱性酒石酸铜溶液中能形成棕黄色配合物,建立了分光光度法测定头孢克洛的新方法。头孢克洛的浓度在32.5~175mg/L范围内与吸光度呈良好的线性关系,线性相关系数为0.994,方法的检出限为0.24mg/L,回收率为96.2%~101.0%。该法可用于胶囊和干混悬剂中头孢克洛含量的测定。 相似文献
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《Analytical letters》2012,45(11):2321-2331
ABSTRACT ABSTRACTA new kinetic spectrophotometric method for the simultaneous determination of concentrations of micro bromide and iodide has been proposed. It is based on their catalytic effects on the reaction of m-cresol purple oxidized by potassium periodate in hydrochloride acid medium. The reaction rate was monitored by measuring the decrease in absorbance at 528nm and the increase in absorbance at 455nm. The total difference in absorbance of the sum of bromide and iodide is identical with determination of bromide was carried out after Cr(VI) oxidized I? to I2, and I2 was removed by extraction with CCI4, and the amount of iodide was measured by subtracting the absorbance change of bromide from the total absorbance change in the presence of bromine and iodide. The optimum conditions influencing the reaction rate were studied. The linear range of determination is 0~4.0μg/ml for Br? and 0~3.0 μg/ml for I?. The detection limits are 0.032μg/ml for Br? and 0.059 μ/ml for I?. The method was successfully applied to the determination of micro amounts of bromide and iodide in food and life samples. 相似文献
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硫代巴比妥酸分光光度法测定消毒液中戊二醛 总被引:4,自引:1,他引:3
在醋酸介质中,硫代巴比妥酸与戊二醛生成缩合产物,此产物在可见光区有最大吸收,籍此可测定微量戊二醛。线性范围为0.5-10.0mg/L,检出限为0.019mg/L。方法灵敏度高,体系简单,操作简便,用本方法测定了消毒液中戊二醛的含量,结果满意。 相似文献
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柯楠因是原小檗碱类生物碱类似物.研究发现,在近生理酸度下柯楠因与寡聚腺嘌呤核苷酸(poly A16)形成双链结构,明显增强DNA嵌入染料赛博绿I(SG I)的荧光.在优化条件下,530 nm处荧光增强与60~600 nmol/L的柯楠因呈线性关系,检测限(3σ)为30 nmol/L.方法选择性好,同倍含量的小檗碱类似物不产生干扰.将所建立的方法用于柯楠因合成样的测定,回收率在92.5%~105%之间,相对标准偏差RSD小于3.1%(n=3). 相似文献
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In this study, a dual wavelength spectrophotometric method was presented on the basis of Cu2+‐NH3 complex decomposition by the citrate ion and the formation of Cu2+‐citrate complex. Sum of the absorbance decreasing at 600 nm (λmax for Cu2+‐NH3 complex) and the absorbance increasing at 750 nm (λmax for Cu2+‐citrate complex) was used in quantification. This method is very selective, fast and inexpensive but relatively less sensitive. The calibration curve is linear in the range of 0.4–8 mmol/L with a detection limit of 0.13 mmol/L. The relative standard deviation (RSD%) for the six repeated determinations (2 mmol/L) is 1.75%. Interference studies of more than twenty common compounds, which are present in the studied samples, were carried out. Only oxalic and ascorbic acids are serious interferes and which interferences were eliminated by simple procedures. Finally, the proposed method was successfully applied in the determination of citric acid content in ORS powder and some fruit juices. 相似文献
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电化学预处理玻碳电极微分计时电位溶出法测定DNA中的嘌呤碱基及DNA浓度 总被引:6,自引:0,他引:6
研究了鸟嘌呤(G)、腺嘌呤(A)、鸟嘌呤核苷、腺嘌呤核苷和变性DNA在电化学预处理玻碳电极上的恒电流微分计时电位溶出行为. 实验结果表明, 用电化学方法预处理玻碳电极操作简单, 效果明显, 预处理玻碳电极对嘌呤及其核苷和DNA的吸附能力大大增强, 用微分计时电位溶出法可以得到灵敏的溶出峰, 溶出峰高(dt/dE)与其浓度在一定范围内呈良好的线性关系, 可用于嘌呤碱基及其核苷的定量检测和DNA浓度的测定. 将该方法应用于酸变性DNA样品中鸟嘌呤与腺嘌呤的同时测定, 选择性好、灵敏度高; 还可获得有关DNA损伤的一些信息. 相似文献