nacl存在下氯化亚锡-十六烷基三甲基氯化铵体系浮选分离铂(ⅱ)、钯(ⅱ)、铑(ⅲ)、金(ⅲ)

pt(ⅱ);pd(ⅱ);rh(ⅲ);au(ⅲ);氯化亚锡;十六烷基三甲基氯化铵;液-固浮选分离     

高频超声照射下血卟啉(HP)对脱氧核糖核酸(dna)结构的影响

超声;血卟啉(hp);损伤;脱氧核糖核酸(dna)     

Eu(OPri)3/Tb(OPri)3共掺杂P(MMA-co-St)

Eu(OPri)3/Tb(OPri)3共掺杂P(MMA-co-St);Eu(OPri)3/Tb(OPri)3;共掺杂;P(MMA-co-St);荧光性能     

双金属配合物PrBi(edta)(NO3)2·6.5H2O的合成、表征与热分解

双金属配合物;铋(Ⅲ);镨(Ⅲ);热分解     

氢化物发生-原子吸收光谱法测定锑(Ⅲ)和锑(Ⅴ)

提出在酸性条件下以氟化钠为掩蔽剂测定锑(Ⅲ),在碱性介质中加入氟化钠消除价态的影响测定锑总量,两者相减求出锑(Ⅴ),实现对锑(Ⅲ)和锑(Ⅴ)的分别测定.研究了在酸性介质中测定锑(Ⅲ)及碱性介质中测定锑总量的条件及共存离子的干扰和消除.工作曲线的线性范围为：0～20 μg·L-1锑(Ⅲ);0～80 μg·L-1锑(Ⅴ),相关系数大于0.999,方法的检出限为0.31 μg·L-1锑(Ⅲ),0.69 μg·L-1锑(Ⅴ).对水样和土壤样品进行了测定,相对标准偏差(RSD)小于5%.     

交联羧甲基魔芋葡甘聚糖对Fe(Ⅲ)和Cr(Ⅲ)的吸附行为研究

研究了交联羧甲基魔芋葡甘聚糖(Crosslinked carboxymethyl konjac glucomannan,CCMKGM)对Fe(Ⅲ)和Cr(Ⅲ)的吸附行为。用FTIR和RAMAN对吸附产物进行表征。结果表明,CCMKGM主要以配位的形式吸附Fe(Ⅲ)和Cr(Ⅲ);吸附在60min内达到平衡,遵从二级动力学方程,其中Cr(Ⅲ)较好地符合粒子内扩散模型;pH对吸附量影响较大,适宜范围为3.0～7.0;CCMKGM对Fe(Ⅲ)的吸附行为符合Langmuir方程,而对Cr(Ⅲ)的吸附行为符合Freundlich方程,其最大吸附量分别为2.7mmol/g和2.2mmol/g(298K);吸附为自发的、吸热的、熵增加过程。CCMKGM对Fe(Ⅲ)和Cr(Ⅲ)的吸附具有吸附容量大、适宜pH范围宽、再生性好等特点,有望将其作为吸附剂用于污水处理及铁和铬的形态分析。     

锰(Ⅱ)、钴(Ⅱ)、铜(Ⅱ)和镉(Ⅱ)与吡哆醇配合物的结构研究

本文合成了吡哆醇与锰(Ⅱ)、钴(Ⅱ)、铜(Ⅱ)和镉(Ⅱ)的配合物。通过元素分析确定了配合物的组成。通达摩尔电导、比移值,紫外光谱、红外光谱和核磁共振谱的测定和分析,推断了配合物的结构。     

硫酸铵-二溴对甲基偶氮羧磺-聚乙二醇-水体系浮选分离铅(Ⅱ)

硫酸铵-二溴对甲基偶氮羧磺-聚乙二醇-水体系浮选分离铅(Ⅱ);铅(Ⅱ);浮选分离;二溴对甲基偶氮羧磺(DBMCSA);聚乙二醇(PEG2000);硫酸铵     

HCOO在Cu(110)、Ag(110)和Au(110)表面的吸附

采用密度泛函理论(DFT)以及广义梯度近似方法(GGA)计算了甲酸根(HCOO)在Cu(110)、Ag(110)和Au(110)表面的吸附. 计算结果表明, 短桥位是最稳定的吸附位置, 计算的几何参数与以前的实验和计算结果吻合. 吸附热顺序为Cu(110)(-116 kJ·mol-1)>Ag(110)(-57 kJ·mol-1)>Au(110)(-27 kJ·mol-1), 与实验上甲酸根的分解温度相一致. 电子态密度分析表明, 吸附热顺序可以用吸附分子与金属d-带之间的Pauli 排斥来关联, 即排斥作用越大, 吸附越弱. 另外还从计算的吸附热数据以及实验上HCOO的分解温度估算了反应CO2+1/2H2→HCOO的活化能, 其大小顺序为Au(110)>Ag(110)>Cu(110).     

2-(p-取代苯乙烯基)-3,3-二甲基-3 H-吲哚盐的合成及性能研究

2,3,3-三甲基-3 H-吲哚氢溴酸盐分别与五种取代苯甲醛在乙醇介质中反应,可得到五种新的2-(p-取代苯乙烯基)-3,3-二甲基-3H-吲哚氢溴酸盐(R=H(3a);Br(3b);MeO(3c);HO(3d);Me_2N(3e)]。研究了化合物3a-e的紫外光谱和Hammett常数的定量关系。以位移增量△λ_p,对△σ(△σ=σ_p-σ_m)作图可得到一条直线,说明它们紫外光谱的取代基效应遵守Hammett方程,即随着取代基给电子能力的增强,吸收带向红移。五个化合物以3e的光谱增感性能最好。     

低频超声波照射下卟啉锰(HP-Mn)配合物对牛血清白蛋白(BSA)损伤的研究

应用紫外-可见(UV-vis)光谱和荧光光谱研究了卟啉-锰(HP-Mn)配合物与牛血清白蛋白(BSA)的相互作用及在超声波作用下HP-Mn对BSA的损伤作用,并探讨了超声波照射时间,HP-Mn浓度,酸度,离子种类和强度等因素对BSA损伤的影响。结果表明,HP-Mn对BSA荧光的猝灭属于静态猝灭,两者主要通过静电作用相互结合,同时也存在着配位作用,作用距离(r)为3.49 nm。另外,在超声波作用下,HP-Mn能够明显损伤BSA。损伤程度随着超声波照射时间的增长,酸度的升高和HP-Mn浓度的增大而增大,但离子种类和强度的影响较为复杂。这一结果为声动力学(SDT)疗法治疗肿瘤应用于临床具有重要的意义。     

Catalytic damage of bovine serum albumin by metronidazole under ultrasonic irradiation in the presence of nano-sized TiO2 powder

In previous work, it was found that the bovine serum albumin (BSA) could obviously be damaged by nano-sized TiO₂ powder as a sonocatalyst under ultrasonic irradiation. In this work, metronidazole (MTZ) was adopted as a sensitizer to intensify the damage of BSA molecules. It was found that the damage degree of BSA molecules in the presence of MTZ was more serious than in the absence of MTZ. That is, under ultrasonic irradiation combined with nano-sized TiO₂ powder, the addition of MTZ could remarkably aggravate the damage to BSA molecules. Meanwhile, the damage degree was also affected by some influence factors, such as ultrasonic irradiation time, ultrasonic irradiation power, MTZ concentration, solution acidity, ionic strength and solution temperature. In addition, the damage site of BSA molecules was also estimated by synchronous fluorescence spectra. It was found that the damage site of BSA molecules was mainly at tyrosine (Tyr) residue.     

Assisted sonodynamic damage of bovine serum albumin by metronidazole under ultrasonic irradiation combined with photosensitive antitumor drug-Amsacrine

By research, it was found that the Amsacrine (AMSA) can interact with bovine serum albumin (BSA). In this work, the AMSA was adopted as a sonosensitizer and the Metronidazole (MET) was used as a sensitizer to further damage BSA molecules under ultrasonic irradiation. It could be concluded that the damage degree of BSA molecules in the presence of AMSA and MET was more serious than in the presence of pure AMSA. That is, MET could aggravate the damage to BSA molecules under ultrasonic irradiation combined with AMSA. Meanwhile, the damage degree of BSA molecules was also influenced by some factors, such as ultrasonic irradiation time, MET concentration and solution acidity. In addition, the damage site of BSA molecules was estimated by synchronous fluorescence spectra. It was found that the tyrosine (Tyr) and tryptophan (Typ) residues were damaged almost averagely. Perhaps, these research results are of great significance for driving sonodynamic method to treat tumor in clinic application.     

高频超声照射下卟啉镓对牛血清白蛋白的损伤

用紫外-可见(UV-Vis)光谱和荧光(FL)光谱研究了高频超声波激活卟啉镓(HP-Ga)配合物对牛血清白蛋白(BSA)的损伤，探讨了超声波照射时间，HP-Ga浓度和酸度等因素对BSA损伤的影响。结果表明，在一定条件下，BSA的损伤程度随着照射时间的增加和HP-Ga浓度的增大而加剧。     

Spectroscopic studies on interaction and sonodynamic damage of metallochlorophyllin (Chl-M (M=Fe, Zn and Cu)) to protein under ultrasonic irradiation

In this paper, the chlorophyll derivatives, metallochlorophyllin (Chl-M) (M=Fe, Zn and Cu) including chlorophyllin iron (Chl-Fe), chlorophyllin zinc (Chl-Zn) and chlorophyllin copper (Chl-Cu), were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. At first, the interaction of Chl-M with BSA was studied by fluorescence spectroscopy. The results show that the quenching mechanism belongs to a static process and among them the affinity of Chl-Fe to BSA is the most obvious. Then, some influence factors on the sonodynamic damage of BSA molecules in the presence of Chl-M under ultrasonic irradiation were also studied. Synchronous fluorescence spectra show that the binding and damage sites of Chl-M to BSA molecule are mainly on the tryptophan (Trp) residues. The generation of ROS in Chl-M sonodynamic process is estimated by the method of Oxidation-Extraction Spectrometry (OEP). This paper may offer some valuable references for the study of the sonodynamic activity of Chl-M and the effect of the central metals. Synchronously, it contributes to the application of Chl-M in SDT for tumor treatment.     

高频超声照射下血卟啉镓配合物对脱氧核糖核酸的损伤

用紫外-可见(UV-Vis)光谱和荧光(FL)光谱研究了高频超声波激活血卟啉镓(HP-Ga)配合物对脱氧核糖核酸(DNA)的损伤，并探讨了超声波照射时间、HP-Ga浓度、溶液酸度和离子强度等因素对DNA损伤的影响。结果表明，在一定条件下，DNA的损伤程度随着超声波照射时间的增加和HP-Ga浓度的增大而加剧，而溶液酸度和离子强度的影响则较为复杂。     

超声照射下血卟啉(HP)对牛血清白蛋白(BSA)损伤的研究

用紫外-可见光谱(UV)和圆二色(CD)光谱研究了超声波激活血卟啉(HP)对牛血清白蛋白(BSA)的损伤, 探讨了超声波照射时间、HP浓度、离子强度和酸度等因素对BSA损伤的影响. 结果表明, 在一定条件下, BSA的损伤程度随着超声波照射时间的增长和HP浓度的增大而增大, 而离子强度和酸度对BSA损伤的影响较为复杂. 这一结果对声动力学(SDT)疗法应用于临床具有重要的意义.     

Study on the sonodynamic activity and mechanism of promethazine hydrochloride by multi-spectroscopic techniques

In this paper, the bovine serum albumin (BSA) was selected as a target molecule, the sonodynamic damage to protein in the presence of promethazine hydrochloride (PMT) and its mechanism were studied by the means of absorption, fluorescence and circular dichroism (CD) spectra. The results of hyperchromic effect of absorption spectra and quenching of intrinsic fluorescence spectra indicate that the ultrasound-induced BSA molecules damage is enhanced by PMT. The damage degree of BSA molecules increases with the increase of ultrasonic irradiation time and PMT concentration. The results of synchronous fluorescence, three-dimensional fluorescence and CD spectra confirmed that the synergistic effects of ultrasound and PMT induced the damage of BSA molecules. The results of oxidation-extraction photometry with several reactive oxygen species (ROS) scavengers indicate that the damage of BSA molecules could be mainly due to the generation of ROS and both (1)O(2) and OH are the important mediators of the ultrasound-induced BSA molecules damage in the presence of PMT.     

Sonocatalytic damage of solute bovine serum albumin by disperse ZnO/porcine dens composite under ultrasonic irradiation

ZnO/Dens composite were prepared with nano-sized ZnO and porcine dens powders by mechanical mixing, liquid boiling, ultrasonic dispersion and heat-treated at 500°C for 50 min. The samples were characterized by X-ray diffraction (XRD) and its sonocatalytic activity was evaluated by the damage of bovine serum albumin (BSA). Furthermore, the effects of ultrasonic irradiation time, ZnO/Dens composite amount, solution acidity and ionic strength on the sonocatalytic damage of BSA were evaluated. The results show that the damage degree of BSA aggravated with the increase of ultrasonic irradiation time, ZnO/Dens composite amount and ionic strength, but weakened with the increase of solution acidity. In addition, the damage site to BSA molecules was analyzed by synchronous fluorescence spectra and the results exhibited that the damage site was mainly at tryptophan (Trp) residue. This study should be helpful to drive sonocatalytic method to treat tumors in clinic application.     

基于光波导分光光谱技术研究蛋白质与亚甲基蓝的竞争吸附行为

通过利用时间分辨光波导分光光谱技术原位测量从蛋白质-亚甲基蓝(MB)混合水溶液吸附到亲水玻璃光波导表面的MB可见光吸收谱,观测到在溶液pH值低于蛋白质等电点时MB与牛血清蛋白(BSA)以及MB与血红蛋白(Hb)存在竞争吸附行为,进一步测得这种竞争吸附行为对蛋白质浓度十分敏感,可以用于简单测定溶液中的蛋白质含量.基于Langmuir等温吸附理论推导出了两种分子竞争吸附的动力学方程,并利用该动力学方程对实验测得的吸光度随时间变化曲线进行了最佳拟合,揭示了玻璃表面吸附的MB分子个数在达到最大值后随时间呈指数衰减,同时得出拟合参数与蛋白质浓度呈准线性关系.