Direct determination of kinetic parameters for diffusion-influenced reactions in solution by analysis of fluorescence decay curves

Journal of Fluorescence - Fluorescence decay curves were synthesized using the Smoluchowski-Collins-Kimball (SCK) model for diffusion-influenced bimolecular reactions so as to simulate measurements...     

RESEARCH ON FLUORESCENCE LIGHT TRANSMISSION IN LARGE AREA SCINTILLATORS

Fluorescence light transmission in scintillators is a very important problem and affects the characters of scintillation counter. Fluorescence light collection in a large area scintillator is discussed in detail. The formulas of fluorescence collection efficiency and the way which improves this collection efficiency are given.     

Diagnostic Application of Fluorescence Spectroscopy in Oncology Field: Hopes and Challenges

Abstract

Cancer is one of the big killers of world population. The majority of cancers are diagnosed at a late stage, making a cure almost impossible. Fluorescence spectroscopy is an emerging diagnostic tool for various medical diseases including premalignant and malignant lesions. Fluorescence spectroscopy is a noninvasive technique and has been applied successfully for the diagnosis of multisystem cancers with high sensitivity and specificity. Fluorescence spectroscopy minimizes the need for repetitive biopsy, which is routine practice for cancer patient follow-up. But there are many aspects of this new diagnostic technique that should be discussed in future research to overcome limitations and challenges faced by this technique for diagnosis of cancers.     

Correction to: Monte Carlo Simulation of the Efficiency of Fluorescence Resonance Energy Transfer,FRET Phenomenon

Journal of Fluorescence -     

Correction to: Parameter Recovery in Frequency-Domain Time-Resolved Fluorescence Spectroscopy; Resolution of the Prototropic Forms of 5-Carboxyfluorescein in the Physiological pH Range

Journal of Fluorescence -     

荧光光谱研究含1,5取代萘刚性生色基双分子膜的相变

用荧光光谱研究了系列双亲物ＣｎＮａｐｈ（１,５）Ｃ６Ｎ＋（ｎ＝１６,１２,１０,８,６和４）聚集体的热致相变,发现荧光强度随温度升高发生了急剧变化,归因于聚集体从凝胶态到液晶态的相变。实验结果表明,尾链长短直接影响聚集体结构和相变温度Ｔｃ高低：尾链长,聚集体堆积密度大,荧光强度强,Ｔｃ高;反之亦反。荧光光谱的实验结果与透射电镜和ＤＳＣ研究结果一致。     

Evaluation of Ni/Mn multilayer samples with glancing-incidence and take-off X-ray fluorescence analysis

A Glancing-Incidence and Take-off X-Ray Fluorescence (GIT-XRF) apparatus with which both incident angle of primary X-ray and take-off angle of fluorescent X-rays can be controlled has been newly developed for characterizing surfaces and interfaces. Using this apparatus, Ni/Mn multilayer samples were evaluated obtaining Take-off-Angle-Dependent X-Ray Fluorescence (TAD-XRF) curves at arbitrary incident angles of primary X-ray. In particular, we could obtain information on the near surface by measuring TAD-XRF curves at the incident angle less than the critical angle of total reflection for primary X-rays.     

A study of tunable laser techniques for remote mapping of specific gaseous constituents of the atmosphere

A comparative study has been made of three laser methods of remotely mapping three atmospheric constituents. It has been found that for NO₂, SO₂ and I₂ Differential Absorption and Scattering has a greater range and sensitivity than Laser-Induced Fluorescence, whilst for SO₂ both techniques are superior to Raman backscattering. However, the sophistication and the difficulty of interpretation associated with the former system indicates that Laser-Induced Fluorescence might be the most suitable technique in a number of situations. In particular, the Fluorescence technique appears to be well suited for mapping of localized sources of specific molecules in the 100 to 1000 m range. An analysis of the fluorescence return signal expected from a localized source has indicated that above a certain peak concentration a distortion of the returned signal could lead to a misinterpretation of both the range and concentration of the source.     

Tm~(3+)掺杂的LaOF和SiO_2纳米体系中荧光光谱温度特性的研究

研究了不同环境温度下晶体LaOF和非晶体SiO2纳米体系中掺杂Tm3+离子的荧光光谱.结果表明,在20~300 K的温度范围内,荧光谱线的宽度、强度以及谱线位置均随温度的升高而变化.发光离子的局域环境直接影响荧光光谱对环境温度的依赖程度:SiO2中发光离子的荧光寿命受温度影响较小,而位于晶相环境LaOF中离子的荧光寿命...     

A New Reversible Colorimetric Chemosensor Based on Julolidine Moiety for Detecting F−

Journal of Fluorescence - We synthesized an original reversible colorimetric chemosensor PDJ...     

城市污水的三维荧光指纹特征

传统表征有机物含量的水质参量如化学需氧量(COD)和生化需氧量(BOD)等只能表示总量,无法展示有机物成分.荧光光谱可以作为一种新型的水质表示方法,它像指纹一样与水样一一对应,被称为水质荧光指纹.采用三维荧光光谱(EEM)技术研究了城市污水荧光指纹特征,结果表明城市污水具有4个典型荧光区,各区的荧光中心、强度以及1区荧光中心λex=280 nm,λem=340 nm与2区荧光中心λex=225 nm,λem=340 nm的荧光强度的比值可以作为城市污水的主要荧光指纹特征.荧光指纹包含了大量污染物信息,通过与城市污水中典型污染物质的荧光光谱的比对,初步确定了各荧光区可能的荧光信号来源.荧光指纹法可表示有机物类型和含量,可作为化学需氧量和生化需氧量等参量的有益补充.     

Fluorescence spectroscopy of synthetic melanin in solution

We report a detailed investigation of fluorescence properties of synthetic eumelanin pigment in solution. A complete set of fluorescence spectra in the near-UV and visible range is analysed. Excitation spectra at a few selected emission energies are also investigated. Our measurements support the hypothesis that fluorescence in eumelanin is related to chemically distinct oligomeric units that can be selectively excited. Fluorescence due to large oligomer systems is spectrally differentiated from that due to monomers and small oligomer systems. Fluorescence excitation measurements show the contribution of 5,6-dihydroxyndole-2-carboxylic acid and 5,6-dihydroxyndole monomers to the emission of small-size oligomers.     

Zur Theorie der nichtstationären Resonanzfluoreszenz an bewegten Molekülen. II. Untersuchungen zur hochauflösenden Resonanzfluoreszenzspektroskopie

Non-stationary Theory of Resonance Fluorescence for Moving Molecules. II. Highly Resolved Fluorescence Spectroscopy With respect to tke Doppler-effect a formula for the spectral energy density of fluorescence radiation of gases (vapours) generated by resonance scattering of short laser pulses is derived. The spectral energy density consists of a coherent and an incoherent part which differs in a characteristic way. The coherent fluorescence radiation reproduces the spectrum of the laser light. Informations about the homogeneous line widths are obtained only from the incoherent spectra. The results of numerical calculations of highly resolved spectra in forward and backward directions under different conditions are discussed.     

利用超短光脉冲研究有机和生物分子发光

利用高重复率Nd:YAG锁模激光输出的倍频光5321Å激发有机染料溶液,通过高速光二极管和示波器组合测量荧光衰减曲线。给出我们用喇曼变换获得适于激发分子的波长,并指出更矩激发波长的获得方法。比较了不同荧光衰变过程测量方法,指出了有机和生物分子中有希望的研究领域。     

Zur Theorie der nichtstationären Resonanzfluoreszenz an bewegten Molekülen. I Räumliche Energiedichte der Fluoreszenzstrahlung

Non-stationary Theory of Resonance Fluorescence for Moving Molecules. I. Spatial Energy Density of the Fluorescence Radiation The time dependence of the spatial energy density of the fluorescence radiation of an ensemble of moving molecules generated by resonance scattering of intense laser pulses is calculated. Particulary the influence of the duration and the intensity of short laser pulses upon the coherent and incoherent part of the fluorescence radiation of a Doppler-broadened gas is studied. The validity of the conception ?inhomogeneous relaxation time”? is discussed. Within the framework of resonance fluorescence scattering the phenomenon of photon echo is treated.     

Fluorene Based Ferric Complex as Colorimetric and Fluorometric Probe for Highly Selective Detection of CN− and S2− Anions

Journal of Fluorescence - A series of new chemosensor molecules bearing benzothiazole-, quinoline- and phthalazine-functionalized fluorene derivatives were synthesized and their complexation...     

The New Society of Fluorescence

Announcement

The New Society of Fluorescence     

Fluorescence Quenching to Study Protein-ligand Binding: Common Errors

A number of recent articles, amongst others several published in the Journal of Fluorescence, use inappropriate fluorescence methodology to determine ligand binding characteristics to (mostly) proteins. In this Letter, several common pitfalls are discussed in relation to two recent publications in the Journal of Fluorescence (Wang et al. (2009) 19:801–808; Ding et al. (2009) 19:783–791). The Author hopes that this contribution helps to prevent a further spread of the incorrect methodology, and results in a reappraisal of those articles already published using similar methodology.