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1.
Measurement of both longitudinal and transverse relaxation interference (cross-correlation) between13C chemical shift anisotropy and13C–1H dipolar interactions is described. The ratio of the transverse to longitudinal cross-correlation rates readily yields the ratio of spectral densitiesJ(0)/JC), independent of any structural attributes such as internuclear distance or chemical shift tensor. The spectral density at zero frequencyJ(0) is also independent of chemical exchange effects. With limited internal motions, the ratio also enables an accurate evaluation of the correlation time for overall molecular tumbling. Applicability of this approach to investigating dynamics has been demonstrated by measurements made at three temperatures using a DNA decamer duplex with purines randomly enriched to 15% in13C.  相似文献   

2.
High-precision heteronuclear three-bond coupling constants including3J(HN, C′),3J(HN, Cβ),3J(Hα,Cγ), and3J(Hβ, C′) are determined for the nonproline residues in uniformly13C-enrichedantamanide,cyclo-(-Val1-Pro2-Pro3-Ala4-Phe5-Phe6-Pro7-Pro8-Phe9- Phe10-), using quantitative numerical 2D NMR spectrum evaluation based on the product-operator formalism. The experimental basis comprises two-dimensional1H,13C-heteronuclear relayed E.COSY spectra [J. M. Schmidt, R. R. Ernst, S. Aimoto, and M. Kainosho,J. Biomol. NMR6,95 (1995)], the multiplet patterns of which are subjected to iterative least-squares 2D multiplet-simulation procedures. Accuracy and precision of the spectrum fit are assessed byFstatistics and analysis of variances (ANOVA) leading to confidence intervals for the optimized spin-system parameters. The long-range J coupling constants obtained and their standard deviations provide the experimental foundation for a later detailed analysis of φ and χ1dihedral-angle equilibrium conformations contributing to the flexible peptide structure.  相似文献   

3.
The NMR spectral densities of a complex motion consisting of a combination of anisotropic overall motion and internal motion have been derived. Two approximations of the equations derived for the cases of slow, Jslow (ω), and fast, Jfast (ω), internal motions are presented. These equations imply that reduction in spectral density of overall motion can be observed if the maxima of internal and overall motions spectral densities versus temperature are well separated, as for fast internal motion. Slow intramolecular motion influences the values of spectral densities of the overall motion if one of the two spins performs a motion, for example a proton in double minimum of the 15N-H?···?N hydrogen bond. The analysis presented reveals small differences between the temperature dependencies of spectral densities of the isotropic and anisotropic overall motions. The theory is illustrated by the 13C protonated carbon spin-lattice relaxation of α-cyclodextrin macromolecule, using the expected motional parameters: D /D ?≈?5 at room temperature and for a fast or slow internal motion.  相似文献   

4.
A solid-state rotational-echo double resonance (REDOR) NMR method was introduced to identify the ?- and ψ-torsion angle from a 1H–15N or 1H–13C′ spin system of alanine-like residues in a selectively, uniformly, or extensively 15N-/13C-labeled peptide. When a Cα(i) or a 15N peak is site-specifically obtainable in the NMR spectrum of a uniformly 15N/13C-labeled sample system, the ψ- or ?-torsion angle specified by the conformational structure of peptide geometry involving 15N(i)–1Hαi15N(i + 1) or 13C′(i − 1)–1HNi13C′(i) spin system can be identified based on 13Cα- or 15N-detected 1Hα15N or 1HN13C REDOR experiment. This method will conveniently be utilized to identify major secondary motifs, such as α-helix, β-sheet, and β-turn, from a uniformly 15N-/13C-labled peptide sample system. When tested on a 13C-/15N-labeled model system of a three amino acid peptide Gly–[U–13C, 15N]Ala–[U–13C, 15N]Leu, the ψ-angle of alanine obtained experimentally, ψ = −40 ± 30°, agreed reasonably well with the X-ray determined angle, ψ = −39°.  相似文献   

5.
Measurement of both longitudinal and transverse relaxation interference (cross-correlation) between13C chemical shift anisotropy and13C–1H dipolar interactions is described. The ratio of the transverse to longitudinal cross-correlation rates readily yields the ratio of spectral densitiesJ(0)/JC), independent of any structural attributes such as internuclear distance or chemical shift tensor. The spectral density at zero frequencyJ(0) is also independent of chemical exchange effects. With limited internal motions, the ratio also enables an accurate evaluation of the correlation time for overall molecular tumbling. Applicability of this approach to investigating dynamics has been demonstrated by measurements made at three temperatures using a DNA decamer duplex with purines randomly enriched to 15% in13C.  相似文献   

6.
The crystal structure and phase transition temperature of [N(C2H5)4]2CuBr4 are studied using X-ray diffraction and differential scanning calorimetry (DSC); measurements revealed a tetragonal structure and the two phase transition temperatures TC of 204 K and 255.5 K. The structural geometry near TC is discussed in terms of the chemical shifts for 1H magic angle spinning (MAS) nuclear magnetic resonance (NMR) and 13C cross-polarization (CP)/MAS NMR. The two inequivalent ethyl groups are distinguishable by the 13C NMR spectrum. The molecular motions are discussed in terms of the spin–lattice relaxation times T in the rotating frame for 1H MAS NMR and 13C CP/MAS NMR. The T results reveal that the ethyl groups undergo tumbling motion, and furthermore that the ethyl groups are highly mobile.  相似文献   

7.
Transverse relaxation-optimized NMR experiment (TROSY) for the measurement of three-bond scalar coupling constant between 1Hαi−1 and 15Ni defining the dihedral angle ψ is described. The triple-spin-state-selective experiment allows measurement of 3JHαN from 13Cα, 15N, and 1HN correlation spectra H2O with minimum resonance overlap. Transverse relaxation of 13Cα spin is minimized by using spin-state-selective filtering and by acquiring a signal longer in 15N-dimension in a manner of semi-constant-time TROSY evolution. The 3JHαN values obtained with the proposed α/β-HN(CO)CA-J TROSY scheme are in good agreement with the values measured earlier from ubiquitin in D2O using the HCACO[N] experiment.  相似文献   

8.
Two new pulse sequences are presented for the recording of 2D13C-HSQC and 3D13C-NOESY-HSQC experiments, containing two consecutive carbon evolution periods. The two periods are separated by az-filter which creates a clean CxHz-quantum state for evolution in the second period. Each period is incremented (in anon-constant-time fashion) only to the extent that the defocusing of carbon inphase magnetization throughJ-coupling with neighboring carbons remains insignificant. Therefore,13C homonuclearJ-couplings are rendered ineffective, reducing the loss of signal and peak splitting commonly associated with long13C evolution times. The two periods are incremented according to a special acquisition protocol employing a13C–13C gradient echo to yield a data set analogous to one obtained by evolution over the added duration of both periods. The spectra recorded with the new technique on uniformly13C-labeled proteins at twice the evolution time of the standard13C-HSQC experiment display a nearly twofold enhancement of resolution in the carbon domain, while maintaining a good sensitivity even in the case of large proteins. Applied to the IIAManprotein ofE. coli(31 kDa), the13C-HSQC experiment recorded with a carbon evolution time of 2 × 8 ms showed a 36% decrease in linewidths compared to the standard13C-HSQC experiment, and theS/Nratio of representative cross-peaks was reduced to 40%. This reduction reflects mostly the typical loss of intensity observed when recording with an increased resolution. The13C-NOESY-HSQC experiment derived from the13C-HSQC experiment yielded additional NOE restraints between resonances which previously had been unresolved.  相似文献   

9.
The edge of the wedge theorem is generalized to the case where one only assumes the existence and equality of the distribution boundary values off ±(z) and all their derivatives on some analytic curveC inR n . Heref ±(z) are holomorphic inR n ±iC, respectively, whereC is a convex cone, andC has its tangent vector inC at every point. Under these assumptions there exists an analytic continuationf(z) holomorphic in some complex neighbourhood of the double cone generated byC. A proof is also given of the connection between the existence of a distribution boundary value and the growth of the holomorphic function near the boundary.  相似文献   

10.
A high resolution diode laser absorption spectrum of the ν9 R Q 0 branch of C2H6 at 822 cm-1 reveals a rotational progression with anomalous spacing and intensity, which is shown to be a result of both l-doubling and torsional splitting of rotational levels, both of which give rise to a J(J+1) energy dependence. From a contour analysis of the spectrum we estimate values for the l-doubling and torsional splitting parameters.  相似文献   

11.
An expression for the free energy of an (001) oriented domain wall of the anisotropic 3D Ising model is derived. The order--disorder transition takes place when the domain wall free energy vanishes. In the anisotropic limit, where two of the three exchange energies (e.g. Jx and Jy ) are small compared to the third exchange energy (Jz ), the following asymptotically exact equation for the critical temperature is derived, sinh(2Jz /k B T c)sinh(2(Jx ?+?Jy )/k B T c))?=?1. This expression is in perfect agreement with a mathematically rigorous result (k B T c/Jz ?=?2[ln(Jz /(Jx ?+?Jy ))?ln(ln(Jz /(Jx ?+?Jy ))?+?O(1)]?1) derived earlier by Weng, Griffiths and Fisher (Phys. Rev. 162, 475 (1967)) using an approach that relies on a refinement of the Peierls argument. The constant that was left undetermined in the Weng et al. result is estimated to vary from ~0.84 at ((Hx ?+?Hy )/Hz )?=?10?2 to ~0.76 at ((Hx ?+?Hy )/Hz )?=?10?20.  相似文献   

12.
Line shift coefficients for five lines of five different isotopomers in the fundamental band of CO in the spectral region near 2058 cm−1were measured using a three channel lead salt diode laser spectrometer. The study includes the linesP(3) of13C17O,R(3) of13C18O,P(9) of12C18O,P(10) of13C16O, andP(21) of12C16O, and covers collisions with N2, O2, H2, D2, He, Ne, Ar, Kr, and Xe. Line shifts of the isotopomers13C16O,12C18O,13C18O, and13C17O were determined for the first time. Within the experimental uncertainty no significant dependence of the shift effect on the isotopomer was found. TheR-branch line under study shows a smaller line shift coefficient than aP-branch line with a similar rotational quantum number. With increasing mass of the noble gas perturber the absolute size of the shift coefficient increases. Moreover self- and nitrogen-broadening coefficients for the isotopomer lines were determined. Compared to previous measurements no significant deviations between different isotopomers were observed.  相似文献   

13.
The emission spectra of C2(d3Πg–a3Πu), CH(A2Δ–X2Π), and CH(B2Σ–X2Π) bands are analysed to measure rotational Trot, vibrational Tvib, and gas temperature Tg from Ar/C2H2 (5–20% C2H2) microwave‐induced plasma (MIP). In case when helium and hydrogen are used in the gas mixture instead of argon, no significant change in Trot is noticed. Both studied temperatures are insensitive in terms of the C2H2 percentage. From CH(0–0, A2Δ–X2Π) band R2 branch lines, two Trot (Trot ~ 520–580 K for J′ = 3–9 and Trot ~ 1,700–1,800 K for J′ = 10–17) are determined. The lower Trot equals the Tg (500–700 K) measured from C2 bands in this study. The H2 Fulcher‐α diagonal bands are recorded as well in the H2/C2H2 mixtures and Trot~750–900 K of the H2 ground state measured. Tvib ~ 6,000 K in Ar/C2H2 MIP is calculated from the integral intensity ratio of C2(2,1) and C2(3,2) bands.  相似文献   

14.
In this paper, we consider generalized holographic and Ricci dark energy models where the energy densities are given as ρ R =3c 2 M pl2 Rf(H 2/R) and ρ h =3c 2 M pl2 H 2 g(R/H 2), respectively; here f(x), g(y) are positive defined functions of the dimensionless variables H 2/R or R/H 2. It is interesting that holographic and Ricci dark energy densities are recovered or recovered interchangeably when the function f(x)=g(y)≡1 or f(x)=Id and g(y)=Id are taken, respectively (for example f(x),g(x)=1−ε(1−x), ε=0or1, respectively). Also, when f(x)≡xg(1/x) is taken, the Ricci and holographic dark energy models are equivalent to a generalized one. When the simple forms f(x)=1−ε(1−x) and g(y)=1−η(1−y) are taken as examples, by using current cosmic observational data, generalized dark energy models are considered. As expected, in these cases, the results show that they are equivalent (ε=1−η=1.312), and Ricci-like dark energy is more favored relative to the holographic one where the Hubble horizon was taken as an IR cut-off. And the suggested combination of holographic and Ricci dark energy components would be 1.312R−0.312H 2, which is 2.312H2+1.312[(H)\dot]2.312H^{2}+1.312\dot{H} in terms of H 2 and [(H)\dot]\dot{H} .  相似文献   

15.
A zero-quantum/double-quantum HNCO(H) constant time experiment is presented for the quantitative evaluation of dipole–CSA cross-correlated relaxation involving the1HN,15N, and13C′ nuclei of the peptide plane. A simple procedure that allows the extraction of cross-correlated relaxation rate constants from intensity ratios of well-resolved doublet components along ω1is described. The experiment is demonstrated on fully13C,15N-labeled ubiquitin.  相似文献   

16.
The Fourier transform infrared gas-phase spectrum of thiazole, C3H3NS, has been recorded in the 600-1400 cm−1 wavenumber region with a resolution around 0.0030 cm−1. Nine fundamental bands (ν5(A′) to ν11(A′), ν15(A″), and ν16(A″)) are analysed employing the Watson model. Ground-state rotational and quartic centrifugal distortion constants as well as upper state spectroscopic constants have been obtained from the fits. A detailed analysis of perturbations identified in the ν11(A′) band at 866.5 cm−1 enables a definitive location of the very weak ν10(A′) and ν14(A″) bands at 879.3 and 888.7 cm−1, respectively. The three levels are analysed simultaneously by a model including Coriolis resonance using an ab initio predicted first order c-Coriolis coupling constant; second and higher order Coriolis parameters are determined. Qualitative explanations in terms of Coriolis resonances are given for a number of crossings observed in ν5(A′), ν6(A′), and ν7(A′) at 1383.7, 1325.8, and 1240.5 cm−1, respectively. The rotational constants, anharmonic frequencies, and vibration-rotation constants (alphas, ) calculated by quantum chemical calculations using a cc-pVTZ and TZ2P basis with B3LYP methodology, have been compared with the present experimental data. The rotation constant differences for each vibrational state, from the ground state values, are closer to experiment from the TZ2P calculations relative to those using cc-pVTZ. The values for ΔJ, ΔJK, ΔK, δJ, and δK are close to experiment with both basis sets.  相似文献   

17.
Given a complex number λ of modulus 1, we show that the bifurcation locus of the one parameter family {f b (z)=λz+b z 2+z 3} b contains quasi-conformal copies of the quadratic Julia set Jz+z 2). As a corollary, we show that when the Julia set Jz+z 2) is not locally connected (for example when z↦λz+z 2 has a Cremer point at 0), the bifurcation locus is not locally connected. To our knowledge, this is the first example of complex analytic parameter space of dimension 1, with connected but non-locally connected bifurcation locus. We also show that the set of complex numbers λ of modulus 1, for which at least one of the parameter rays has a non-trivial accumulation set, contains a dense G δ subset of S 1. Received: 22 September 2000 / Accepted: 16 January 2001  相似文献   

18.
The possible existence of complexes formed by the C60 fullerene or its derivatives with transition metals interacting with the carbon cage via η6−π type bonding is discussed. The derivatives C60 R 6 of the C60 fullerene (R = −, H, F, Cl, Br, CN) are analyzed using the density functional method within the Perdew-Burke-Ernzerhof approximation. In these molecules, the R groups are attached to carbon atoms located in the α positions with respect to the common hexagon of the C60 fullerene. The structure and electron configuration of complexes formed by these molecules with Cr(C6H6), Cr(CO)3, MoC6H6, and Mo(CO)3 particles are modeled. The “dimer” systems C60R6-M-R 6C60 (M = Cr, Mo, R =-, H, F) are investigated in which two fullerene molecules interact via a transition-metal atom. It is found that the introduction of six R groups in the α sites with respect to the common hexagon of C60 favors the formation of complexes of these derivatives of the C60 fullerene with the Cr(C6H6), Cr(CO), Mo(C6H6), and Mo(CO)3 particles in which η6-π type bonds arise between the metal and the atoms of the hexagon fringed with the R groups. It is also demonstrated that analogous complexes with a “bare” C60 fullerene are possible, but they are significantly less stable. The (C6H6) M-R 6C60 R 6-M (C6H6) complexes of particles M(C6H6) (M= Cr, Mo) and derivatives R 6C60 R 6 (R =-, H, F, Cl, Br) are studied. In the R 6C60 R 6 molecule, six R groups are located in the α sites with respect to the common hexagon of the C60 fullerene and six other groups fringe the opposite hexagon. The obtained results can be applied to planning synthesis of new complexes that C60 fullerene derivatives can form with transition metals. Original Russian Text ¢ E.G. Gal’pern, A.R. Sabirov, I.V. Stankevich, 2007, published in Fizika Tverdogo Tela, 2007, Vol. 49, No. 12, pp. 2220–2223.  相似文献   

19.
The fourth GeH stretching overtone band of monoisotopic H370GeD at 9877 cm−1 has been recorded by ICLAS VeCSEL technique with a path length equivalent to 26 km. The (500 A1/E) band has been observed and rotationally analyzed up to J=18. The band was shown to be essentially unperturbed except some high J states. The assigned transitions were fitted, with σ(Fit) ca. 6.5 × 10−3 cm−1. While eight refined parameters were needed up to Jmax=14, six quartic centrifugal distortion constants were refined in addition for the larger body of 829 data with Jmax=18. The (500 A1/E) parameters of H370GeD perfectly fulfill the theoretical relations valid at the local mode limit, and they fit into the series for other (n00 A1/E) levels (n=2, 3, 6, 7, and 8). A transition moment ratio M(A1):M(E)=0.25 was found to be in best agreement with the observed spectrum, only high J (J?13) transitions being evidently sensitive to this ratio.  相似文献   

20.
The maximum value of hysteresis loss EhMAX due to the itinerant-electron metamagnetic (IEM) transition of La(FexSi1−x)13 and the partially substituted compounds La1−zCez(Fe0.86Si0.14)13 and La1−zPrz(Fe0.86Si0.14)13 increases when the magnetocaloric effects (MCEs) become large. It should be noted that the reduction of EhMAX without the decrease of large MCEs is achieved in La1−zCez(Fe0.86Si0.14)13 and La1−zPrz(Fe0.86Si0.14)13. For both the compound systems mentioned above, the critical temperature T0 for the IEM transition decreases and the difference between T0 and the Curie temperature TC becomes larger with decreasing TC. These results are consistent with the magnetic phase diagram of La(Fe0.86Si0.14)13 under hydrostatic pressure. Consequently, the reduction of EhMAX in La1−zCez(Fe0.86Si0.14)13 and La1−zPrz(Fe0.86Si0.14)13 is closely related with the magnetovolume effects.  相似文献   

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