结构光三维成像系统的计算机仿真

基于相位测量轮廓术和摄像机模型,提出一种结构照明三维成像系统的高精度计算机仿真算法。对于给定的物体三维模型,首先根据系统结构,采用Z缓冲技术消除遮挡和阴影部分,得到与摄像机像素点对应的物体表面采样点三维坐标,再使用统一的数学模型和方法处理投影过程。根据摄像机及投影仪的内、外参数,最终得到了摄像机像素点、物体表面采样点和投影仪像素坐标三者之间的对应关系,从而实现了结构照明三维成像系统仿真。为实际系统的结构设计、调整和参数校正提供了参考。     

隐失驻波全内反射荧光显微术的数值计算

从理论上详细计算了在全内反射的条件下，两束入射光产生的隐失干涉场(即隐失驻波)的强度分布，并分析了其不同于传统传播波干涉场的特点。同时使用数值模拟证明了利用隐失干涉场，即隐失驻波的激发方式，可以提高系统的分辨力，在横向实现超经典分辨的荧光成像。具体的分析表明，两束光以相等的角度入射，同时振幅相等，偏振态相同，所形成干涉条纹的反衬度最高，此时成像系统的有效点扩展函数最优化；入射介质1的折射率越大，隐失干涉场的空间周期越短(空间频率越高)，其对应的调制点扩展函数中心瓣的半峰全宽越小，可能分辨更小的物体，但同时旁瓣的强度也增强，最终成像的分辨力受两者的共同制约。     

Computer Simulation of a 3-D Sensing System with Structured Illumination

A computer simulation system of three-dimensional sensing with structured illumination is presented. It includes the generation of deformed fringe patterns from 3-D shapes and the reconstruction of 3-D shapes, conversely. Some experimental results of the deformed fringe pattern and the reconstructed object shapes are presented. We have also discussed the effect of some major system parameters on the measurement results and considered how to correct these parameters according to the measurement result of the standard plane. Using this simulation system, the major system parameters: environmental conditions, measurement accuracy and algorithm evaluation of the 3-D shape measurement system based on PMP, FTP, SPM, etc., can be researched. © 1997 Elsevier Science Ltd. All rights reserved.     

The Problem of Subrayleigh Resolution in Interference Microscopy

A method allowing subwavelength resolution within the framework of optical interferometric microscopy is proposed. It is shown that overcoming the diffraction limit is not a necessary requirement. Generally speaking, subrayleigh resolution and overcoming the diffraction limit are basically different concepts. The method developed uses the all-parameter modulation of the light source and separation of the phase shifts generated due to different reasons. In this method, a topological phase technique is used to separate the phases and therefore determine the corresponding characteristics of an object. The approach developed provides for a way to create a new type of optical devices. Within the framework of a uniform measuring procedure these devices make possible determining both geometrical and material parameters of the object under study.     

10 nm Spatial Resolution Fluorescence Imaging of Single Molecules by Near-Field Scanning Optical Microscopy Using a Tiny Aperture Probe

We demonstrate high-resolution fluorescence imaging of single molecules using near-field scanning optical microscopy (NSOM) with a tiny aperture probe for two different wavelengths in visible range in the illumination mode of operation. The spatial resolutions obtained at both excitation wavelengths were almost the same and the highest resolution realized was about 10 nm. To discuss the achievable resolution in aperture NSOM, we also employed a computer simulation by the finite-difference time-domain method for various aperture sizes and wavelengths. The resolution of 10 nm is predicted to be contributed by the single peak of localized near-field light around the rim of the aperture.     

Detection of Single Quantum Dots in Model Systems with Sheet Illumination Microscopy

Single molecule detection and tracking provides at times the only possible method to observe the interactions of low numbers of biomolecules, inlcuding DNA, receptors and signal mediating proteins in living systems. However, most existing imaging methods do not enable both high sensitivity and non-invasive imaging of large specimens. In this study we report a new setup for selective plane illumination microscopy (SPIM), which enables fast imaging and single molecule tracking with the resolution of confocal microscopy and the optical penetration beyond 300 μm. We detect and report our instrumental figures of merit, control values of fluorescence properties of single nano crystals in comparison to both standard widefield configurations, and also values of nanocrystals in multicellular “fruiting bodies” of Dictyostelium, an excellent control as a model developmental system. In the Dictyostelium , we also report some of our first tracking of single nanocrystals with SPIM. The new SPIM setup represents a new technique, which enables fast single molecule imaging and tracking in living systems.     

High Resolution B-Type Delayed Fluorescence of Dibenzanthracene in PMMA

B-Type delayed fluorescence of 1,2,3,4-dibenzanthracene in PMMA was experimentally observed for the first time. Dibenzanthracene molecules were exerted in a two-step process. In the first step, an excited singlet S₁ is created, which undergoes intersystem crossing to T₁ then T-T absorption creates an excited triplet dibenzanthracene molecule, which returns to the first exerted singlet level by intersystem crossing. The recreated first excited singlet of dibenzanthracene decays back to the ground state by emitting this new type of delayed fluorescence.     

应用小波边缘检测技术分析荧光寿命显微像

研究了小波图象边缘检测技术在荧光寿命显微像分析中的应用，利用小波分析的多尺度特性进行了荧光寿命显微像的边缘提取，结果表明，这种方法非常有效。     

Spatial Resolution of Acoustic Microscopy in the Visualization of Interfaces inside a Solid

Acoustical Physics - The volumetric visualization of materials and inner boundaries of a joint is one of the fields in which long-focus high-frequency beams are used. Visualization is carried out...     

数字全息显微系统结构参量对再现像质的影响

为了获取高质量的数字全息显微再现像,分析了同轴相移数字全息显微系统结构参量对再现像质的影响.首先经过计算得出理想成像时,像空间物光波频率完全由物体面形结构频率和系统放大倍率决定.然后基于显微成像时物光波的所有频率分量都应该被有效记录的分析,得出在同轴相移系统中放大倍率必须使物光波的空间频率缩小到满足采样要求;并得出在相...     

复眼透镜阵列应用于均匀照明系统的特性研究

研究了复眼透镜阵列实现均匀照明的原理,模拟分析了复眼透镜的列数、入射光发散角、光源尺寸对照明效果的影响。仿真表明,入射光的发散角越小,光源尺寸越小,照明效果越好,而且适当地选取复眼透镜的列数能提高照明效果。     

Direct Observation of Lipid Domains in Free-Standing Bilayers Using Two-Photon Excitation Fluorescence Microscopy

The direct observation of temperature-dependent lipid phase equilibria, using two-photon excitation fluorescence microscopy on giant unilamellar vesicles (GUVs) composed of different lipid mixtures, provides novel information about the physical characteristics of lipid domain coexistence. Physical characteristics such as the shape, size, and time evolution of different lipid domains are not directly accessible from the traditional experimental approaches that employ either small and large unilamellar vesicles or multilamellar vesicles. In this review article, we address the most relevant findings reported from our laboratory regarding the direct observation of lipid domain coexistence at the level of single vesicles in artificial and natural lipid mixtures. In addition, key points concerning our experimental approach will be discussed. The unique advantages of the fluorescent probe 6-dodecanoyl-2-dimethylaminonaphthalene (LAURDAN) under two-photon excitation fluorescence microscopy is particularly addressed, especially, the possibility of obtaining information on the phase state of different lipid domains directly from the fluorescent images.     

Locating of Nucleic Acid Intercalators in Yeast Cells by Image Analysis Combined Fluorescence Microscopy

Intracellular distribution in the intact (not fixed) Saccharomyces cerevisiae cells of the nucleic acid intercalators (NAI) was studied using fluorescence microscopy combined with computer image analysis (ImageJ software, NIH, USA). Three NAI—the anthracycline anticancer drug doxorubicin (DR) along with the nucleic acid dyes ethidium (E) and 4′,6-diamidino-2-phenylindole (DAPI)—were used. Staining pattern and ImageJ quantitative analysis data provided evidence that all three NAI were located in the nuclei and in the mitochondria. DR and E, in contrast to DAPI, may be bound to not only DNA, but to the mitochondrial membranes as well. Experiments on the combined application of DR+DAPI and E+DAPI have shown potential competition of DAPI with DR and E for binding sites in the nuclear and mitochondrial DNA. With the approach presented herein, the yeast cells of S. cerevisiae can be used as a model for locating intracellular sites of the fluorescing nucleic acid intercalators. This model may be of help in designing new DNA-targeted drugs and in preliminary studies of their interaction with eukaryotic cells.     

Nonlinear Lattice Effect on Ground State of One-Dimensional Electron-Phonon System

We study the nonlinear lattice effect on the ground state in a one-dimensional spinless Holstein model with nonadiabitical coupling and squeezing by means of the parameter variational approach. Our results show that the introduction of a hard quartic term in the lattice potential increases the ground state energy of the system when electron-phonon coupling is strong, and the increment is sensitive to the magnitude of the lattice quartic force constant. In this case the nonlinear lattice effects should be taken into account to describe satisfactorily some physical properties of the coupling system.     

Fluorescence Spectroscopy and Confocal Microscopy of the Mycotoxin Citrinin in Condensed Phase and Hydrogel Films

The emission spectra, quantum yields and fluorescence lifetimes of citrinin in organic solvents and hydrogel films have been determined. Citrinin shows complex fluorescence decays due to the presence of two tautomers in solution and interconversion from excited-state double proton transfer (ESDPT) process. The fluorescence decay times associated with the two tautomers have values near 1 and 5 ns depending on the medium. In hydrogel films of agarose and alginate, fluorescence imaging showed that citrinin is not homogeneously dispersed and highly emissive micrometer spots may be formed. Fluorescence spectrum and decay analysis are used to recognize the presence of citrinin in hydrogel films using confocal fluorescence microscopy and spectroscopy.     

The Effect of Incoherent Population Pumping on Squeezing in Resonance Fluorescence

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The Effect of Incoherent Population Pumping on Squeezing in Resonance Fluorescence

The effect of incoherent population pumping on the steady-state population inversion and the quadrature squeezing spectra produced in the resonance fluorescence of a two-level atom is investigated. In the presence of incoherent population pumping, the steady-state population inversion is increased for small frequency detuning but is not changed for large frequency detuning. For resonant excitation at low intensities, the weak incoherent pumping degrades the degree of the squeezing and shifts the position of the maximum squeezing; for off-resonant excitation at strong intensities, the weak incoherent pumping hardly changes the squeezing spectra. But when the incoherent pumping is strong the squeezing may be completely destroyed for both cases.     

The Nonlinear Effects of a Kerr-Resonator Optomechanical System

Optomechanics describes the interaction of optical field with mechanical systems via radiation pressure which provides the interface between photons and phonons. Recently, the study of nonlinear behavior of optomechanical system attracts much attention. This paper studies theoretically the nonlinear behavior due to the presence of a Kerr nonlinear crystal inside an optomechanical microcavity. We first give an effective Hamiltonian of the system, then by solving the amplitude of both the optical and mechanical operators, two kinds of nonlinear effects, the bistability and the parametric gain on the mechanical mode of the system, are investigated. Moreover, we find that the intensity of the mechanical mode could be controlled in the presence of the nonlinear media.     

Reactive Phenanthrene Derivatives as Markers of Amino Groups in Fluorescence Microscopy of Surface Modified Micro-Zeolite L

Journal of Fluorescence - Two reactive phenanthrene derivatives, 4-(1H phenanthrol [9,10-d] imidazole-2-yl) benzaldehyde (PIB) and 6,9-dimethoxyphenanthro[9,10-c]furan-1,3-dione (PA) with high...     

Effect of the Dark Illumination Intensity on the Characteristics of Surface Waves Propagating Along the Interface between Photorefractive and Nonlinear Kerr Crystals

Russian Physics Journal - Nonlinear surface waves of the TM polarization propagating along the interface between crystals with Kerr and photorefractive diffusion nonlinearities are considered. It...