共查询到20条相似文献,搜索用时 0 毫秒
1.
Diffey B 《Photochemistry and photobiology》2008,84(2):371-375
Determining the variability of solar UV exposure of different members of a population by direct measurement demands high compliance over an extended period of time by a large number of people. An alternative approach is to model the variables that affect personal exposure and this is the basis of the method reported here, which uses a random sampling technique to explore variability of exposure at different times of the year by habitués. It is shown that there are large variations in daily personal erythemal exposure, more so for indoor workers living in northern Europe than those resident in Florida, which are due not only to seasonal changes in ambient, but just as importantly to seasonal variation in behavior. Not surprisingly, holiday and summer weekend exposure account for the largest daily UV doses. Northern Europeans who take their summer vacation in Florida can double their exposure during this period compared with holidaying at home and this illustrates just how important sun protection measures should be during recreational exposure in areas of high insolation if the annual UV burden is to be sensibly controlled. 相似文献
2.
Huebner JD Young DL Loadman NL Lentz VJ Wiegand MD 《Photochemistry and photobiology》2006,82(6):1656-1661
We examined age-related vulnerability of Daphnia magna to UV-B (ultraviolet-B radiation, 280-320 nm), the age-related relationships between UV-B dosage and reproduction, and the effects of duration of PRR (photorecovery radiation) on survival and fecundity. Animals were exposed to 0, 6, 9 or 12 h UV-B (peak emission 312 nm) at 1, 2, 3 or 4 days of age and followed to 12 days. Survival decreased with increasing exposure to UV-B, but increased with age at exposure. Duration of UV-B, but not age at exposure, decreased offspring production. Survival increased with an increase in duration of PRR for animals of all ages; however, the effect was not age related. Three-day-old animals were exposed to 6 h of UV-B followed by 0-600 min of PRR. Survival was greatest in controls, lowest in the group that received no PRR and similar for all other groups. Most mortality occurred within the first 72 h postirradiation. Offspring production was highest in light controls, lowest in the UV-B-exposed group that received no PRR and not significantly different among other groups. This study demonstrates the need to consider age when examining the effects of UV-B on zooplankton and the need to monitor responses over a sufficient length of time. 相似文献
3.
Exposure of the skin to UV radiation can lead to a local infiltration of neutrophils. Not much is known on whether the infiltration of neutrophils in the irradiated skin is UV source dependent. In this study we compared different UV sources (solar-simulated radiation [SSR], narrowband [NB]-UVB, broadband [BB]-UVB and UVA1) in their potency to induce neutrophil infiltration in normal human skin after exposure to two times the minimal erythema dose of UV radiation. Biopsies were collected from irradiated buttock skin 6 and 24 h after irradiation and from nonirradiated skin. The presence, distribution and amount of skin-infiltrated neutrophils were determined using immunohistochemical staining. Analysis revealed that SSR was most effective in inducing neutrophil infiltration. NB-UVB gave a neutrophil influx pattern similar to that seen with SSR but in smaller numbers. BB-UVB and UVA1 were far less potent in inducing neutrophil infiltration compared with SSR or NB-UVB. Our findings indicate that neutrophil infiltration in the UV-irradiated skin is UV source dependent. When the spectra emitted by the different UV sources were compared UVB seemed to be more effective than UVA in inducing neutrophil infiltration. Furthermore, our results suggest that longer wavelengths within the UVB range are mostly responsible for the infiltration of neutrophils in the UV-irradiated skin. 相似文献
4.
The survival of organisms depends on their ability to adapt to their environment, one important aspect of which is the daily cycle of day and night. During the day, organisms use a variety of strategies to protect themselves from deleterious ultraviolet (UV) wavelengths of sunlight. Among those strategies could be timing of UV-sensitive cellular processes to occur at night to avoid UV-induced damage. We tested whether the unicellular alga Chlamydomonas reinhardtii uses this strategy by measuring the survival of cells following exposure to UV radiation at different phases of the day. Chlamydomonas cells displayed a rhythm of survival from UV radiation where the most sensitive phases occurred during the end of the day and at the beginning of the night. This phase of sensitivity corresponds to the time of nuclear division. The rhythm continues in constant light indicating control by a circadian clock. The results presented here suggest a hypothesis of how circadian clocks may have evolved; a temporal program whereby light-sensitive processes are timed to avoid sunlight-induced damage would be advantageous and therefore selected. 相似文献
5.
To assist standardization of procedures, facilitate comparisons, and help guide research efforts to optimally inform development of appropriately targeted interventions, there is a need to review methods used to quantify child and adolescent solar ultraviolet radiation (UV) exposure, related outdoor activities and sun-protective practices. This holistic approach is essential for comprehensive research that will provide all-inclusive, informative and meaningful messages for preventive measures of harmful UV exposure. Two databases were searched and 29 studies were retrieved, and these studies report measurement or assessment techniques documenting UV exposure patterns and related outdoor activities. Polysulfone film badges were the main measurement instrument used in 10 studies, with questionnaire, survey data, observation, a model, electronic dosimeters, biological dosimeters, colorimeter and UV colouring labels used in the remaining studies. Methods used to record activities included self-report, parental report, a logbook and observation. Measurement duration and unit of UV exposure varied in most studies, but a method common to 15 studies was measured UV exposure as a percentage of ambient UV. The studies reviewed do not provide sufficient information for the development and evaluation of targeted youth sun protection programs. Studies are required which document precise UV exposure, concurrent activities and sun protection usage for children and adolescents. 相似文献
6.
O'Riordan DL Stanton WR Eyeson-Annan M Gies P Roy C 《Photochemistry and photobiology》2000,71(1):60-64
Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation (UVR) exposure measured by polysulfone dosimeters worn on the wrist was compared with a measure of estimated exposure using a diary based on recall at the end of the 4 day study period and ambient dosimetric measures. Results of the study indicate that the relative UVR exposures expressed as a fraction of daily total ambient received during the 4 day period by young children and mothers are similar. A high level of association was obtained for the estimated levels of exposure between young children and their mothers. Moderate correlations were evident for dosimeter readings of mother and child on weekends with no significant association on weekdays. The association between estimated exposure and dosimeter readings was poor and needs improvement. This may be achieved by greater consideration of structural and environmental factors that influence the levels of UVR exposure received by individuals and by increasing the level of specificity in the measurement instruments. Methodological issues such as recall of exposure after several days, quantity of sun exposure and more precise measurement of grades of exposure when outside may have more impact than previously expected, and further information is being sought with a larger sample for exposure during the summer months. 相似文献
7.
Solar UVB radiation (280-320 nm) is known to have detrimental effects on marine phytoplankton. Associated with the seasonal ozone hole in Antarctica, stratospheric ozone depletion occasionally influences the sub-Antarctic (Beagle Channel, Argentina) region, enhancing levels of UVB. The primary objective of this work was to study the effects of several (i.e. 6-10) days of exposure to UVB on the taxonomic composition and photosynthetic inhibition of local phytoplankton communities. For different light treatments, fixed-depth incubations placed in an outdoors water tank were compared with incubations in 1900 L mesocosms, where vertical mixing was present. Phytoplankton growth was inhibited by UV radiation (UVR) in fixed-depth experiments but not in the mixed mesocosms. Under fixed and mixed conditions alike, photosynthesis was significantly inhibited by UVB at the beginning of the experiment but no longer after several days of exposure, suggesting that cells had acclimated to radiation conditions. There was a change in species composition in response to UVR exposure in both experiments, which likely explained acclimation. In the community exposed to fixed conditions this change was from a phytoflagellate-dominated assemblage to a community with high relative abundance of diatoms after 6 days of exposure. UVA was responsible for most of the observed growth inhibition; however, the reduction in photosynthesis was produced by UVB. The reasons behind this variability in responses to UVR are associated with species-specific sensitivity and acclimation, and the previous light history of cells. In the community exposed in mesocosms, an assemblage codominated by phytoflagellates and diatoms was observed at the beginning of the experiments. After 10 days of exposure, green algae (Eutreptiella sp.) had increased, and phytoflagellates were the dominant group. The synthesis of mycosporine-like amino acids (MAAs), antioxidant enzymes and photosynthetic antenna pigments, in relation to repair and protection processes, may explain the reduced inhibition of both growth and photosynthesis that was observed in the phytoplankton community after several days of exposure. For environments such as the Beagle Channel seasonally exposed to the ozone hole, the results obtained from the fixed-depth experiments show that species can cope with UVR by means of MAA synthesis, while mixing would primarily promote a change in species composition and defense strategies. 相似文献
8.
P J Neale 《Journal of photochemistry and photobiology. B, Biology》2001,62(1-2):1-8
Spectral ultraviolet (UV) irradiance, water column attenuation and biological weighting functions for inhibition of phytoplankton photosynthesis have been measured for the Rhode River, a subestuary of the Chesapeake Bay. Together, these measurements can be used to estimate UV effects on water column production, but each factor shows a significant range of variability even just considering summer time conditions. A sensitivity analysis of UV inhibition is described which assesses the effect of this variation for different combinations of 28 irradiance spectra, 8 biological weighting functions (BWFs) and 16 water column irradiance profiles. Over all combinations, production averaged about 84% relative to potential production in the absence of UV effects. For a few combinations, relative production was as low as 67%, or as high as 97%, but for most combinations the range was 75-95%. Variations in the sensitivity of the phytoplankton assemblage, i.e. the BWF, and optical properties, represented by a transparency ratio of biologically effective UV to photosynthetically available radiation (PAR), had large effects on water column production. A simple relationship for UV inhibition of water column production is developed based on inhibition at the surface and the ratio of UV and PAR transparency. 相似文献
9.
Michael L. Everett Gar B. Hoflund 《Journal of polymer science. Part A, Polymer chemistry》2005,43(3):552-561
The chemical alteration of poly(tetrafluoroethylene) Teflon by vacuum ultraviolet radiation (VUV) (115–400 nm) has been examined with X‐ray photoelectron spectroscopy (XPS). The initial F/C atom ratio of 1.98 decreases to 1.65 after a 2‐h exposure. The F/C atom ratio is further reduced to a steady‐state value of 1.60 after a 74‐h exposure. The high‐resolution XPS C1s data indicate that new chemical states of carbon form as F is removed and that the relative amounts of these states depend on the F content of the near‐surface region. The states are most likely due to C bonded only to one F atom, C bonded only to other C atoms, and C that has lost a pair of electrons through the emission of F?. The exposure of the VUV‐damaged surface to research‐grade O2 results in the chemisorption of a very small amount of O, and this indicates that large quantities of reactive sites are not formed during the chemical erosion by VUV. Further exposure to VUV removes this chemisorbed oxygen. A comparison of the XPS data indicates that the mechanisms of chemical alteration by VUV radiation and hyperthermal (~5 eV) atomic oxygen are different, as expected, because the excitation sources are quite different. © 2004 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 552–561, 2005 相似文献
10.
Filippini M Ortelli C Svercel M Bagheri HC 《Journal of photochemistry and photobiology. B, Biology》2011,103(3):234-242
Ultraviolet radiation is an important environmental constraint on the evolution of life. In addition to its harmful effects, ultraviolet radiation plays an important role in generating genetic polymorphisms and acting as a selective agent. Understanding how prokaryotes cope with high radiation can give insights on the evolution of life on Earth. Four representative filamentous bacteria from the family Cytophagaceae with different pigmentation were selected and exposed to different doses of UVC radiation (15-32,400Jm(-2)). The effect of UVC radiation on bacterial survival, growth and morphology were investigated. Results showed high survival in response to UVC for Rudanella lutea and Fibrisoma limi, whereas low survival was observed for Fibrella aestuarina and Spirosoma linguale. S. linguale showed slow growth recovery after ultraviolet exposure, R. lutea and F. limi showed intermediate growth recovery, while F. aestuarina had the fastest recovery among the four tested bacteria. In terms of survival, S. linguale was the most sensitive bacterium whereas R. lutea and F. limi were better at coping with UVC stress. The latter two resumed growth even after 2h exposure (~10,800Jm(-2)). Additionally, the ability to form multicellular filaments after exposure was tested using two bacteria: one representative of the high (R. lutea) and one of the low (F. aestuarina) survival rates. The ability to elongate filaments due to cell division was preserved but modified. In R. lutea 10min exposure reduced the average filament length. The opposite was observed in F. aestuarina, where the 5 and 10min exposures increased the average filament length. R. lutea and F. limi are potential candidates for further research into survival and resistance to ultraviolet radiation stress. 相似文献
11.
Dose response for ultraviolet radiation A-induced focal melanocytic hyperplasia and nonmelanoma skin tumors in Monodelphis domestica 总被引:2,自引:0,他引:2
Ley RD 《Photochemistry and photobiology》2001,73(1):20-23
Four groups of 30 dorsally shaved opossums (Monodelphis domestica) were exposed to graded doses of ultraviolet radiation A (UVA) (320-400 nm) three times per week for 90 weeks. Animals were monitored for the appearance of focal melanocytic hyperplasia (FMH) and nonmelanoma skin tumors (NMST) during the course of the exposures and for an additional 20 weeks following termination of exposures. FMH is the putative precursor for melanoma in the opossum. The lowest dose of UVA (2.5 x 10(3) J/m2) used in this study was selected based on the action spectrum for the induction of melanoma in a fish model. The prediction was that 2.5 x 10(3) J/m2 would induce FMH in the opossum if the action spectra for the induction of FMH in the opossum and melanoma in the fish were the same. The highest UVA dose was 2.5 x 10(5) J/m2. Only the highest dose of UVA gave a statistically significant induction of FMH and NMST in the opossum. As in previous studies, the FMH appeared earlier than the NMST during the course of exposures and the final prevalence of FMH was lower than the final prevalence of NMST. Overall, the results of this study indicate that the efficacy of UVA to induce FMH in the opossum is not as great as would be predicted from the action spectrum for melanoma induction in a fish model. 相似文献
12.
Gao N Hu LW Gao Q Ge TT Wang F Chu C Yang H Liu Y 《Photochemistry and photobiology》2012,88(3):736-743
Measurements were conducted at San Ya, China (18.4°N, 109.7°E, altitude 18 m) to investigate the diurnal variation of ocular exposure to ultraviolet (UV) radiation. The experimental apparatus was composed of a manikin and a dual-detector spectrometer to simultaneously measure ocular and ambient UV data. The experimental apparatus was rotated clockwise to simulate three different types of exposure. When the manikin was facing into the sun, the ocular exposure to UV radiation on a summer day was bimodally distributed. The maximum ocular UV irradiance occurred at solar elevations of around 40° and 50° for UVA and UVB respectively. The spectral irradiances were measured at specific wavelength to obtain the ocular biologically effective UV (UV(BE) ) irradiances for photokeratitis, photoconjunctivitis and cataract, and the UV index (UVI) was calculated at the same time point for comparison. When the manikin faced the sun, the maximal ocular UV(BE) irradiance values were obtained at the solar elevation where the UVI value was 8. The results of this study showed that protection against ocular overexposure during outdoor activities should be taken not only at noon but also at other times. 相似文献
13.
14.
Comparison of action spectra for acute cutaneous responses to ultraviolet radiation: man and albino hairless mouse 总被引:3,自引:0,他引:3
Abstract The hairless mouse has been used as an experimental model for photocarcinogenesis for about 20 years. Although the carcinogenesis action spectra for mice and man are not known, acute responses to ultraviolet radiation (UVR) in the biologically active UVB and UVC region (wavelengths below 320 nm) can be compared. Vascular response (predominantly edema) action spectra for monochromatic radiation in the Skh:HR-l (albino hairless) male mouse were determined. These action spectra were found to be very similar to the human erythema action spectrum that had been developed using the same monochromator. The accuracy of this experimentally derived action spectrum was tested with a series of polychromatic source spectra. The mice were exposed to radiation from a long arc Xe lamp filtered by varying thicknesses of Schott WG320 filters, which yielded a wide range of biologically effective spectra. Spectral irradiance measurements, when weighted with the mouse edema and human erythema action spectra and multiplied by the irradiation time required to elicit a threshold response (edema), yielded a constant weighted dose regardless of irradiation spectral quality. The integrated effective dose was approximately 200 J/m2 of 297 nm equivalent energy, agreeing with requirements for the monochromatic 297 nm dose in the mice as well as for minimal human erythema. These data suggest a commonality in the UVR chromophores of mice and men as they relate to the acute responses described, and a direct additivity of effectiveness from the UVR components in a polychromatic beam, at least over the portion of the UVR spectrum tested (λ > 295 nm). 相似文献
15.
McLachlan-Burgess A McCarthy S Griffin C Richer J Cutler RG Pandey S 《Applied biochemistry and biotechnology》2006,135(2):159-177
Radiation therapy has been used in the treatment of a wide variety of cancers for nearly a century and is one of the most
effective ways to treat cancer. Low-dose ionizing radiation (IR) can interfere with cell division of cancer and normal cells
by introducing oxidative stress and injury to DNA. The differences in the response to IR-induced DNA damage and increased
reactive oxygen species between normal human fibroblasts (NHFs) and cancerous SHSY-5Y cells were considered. H2AX staining
and comet assays revealed that NHF cells responded by initiating a DNA repair sequence whereas SHSY-5Y cells did not. In addition,
NHF cells appeared to quench the oxidative stress induced by IR, and after 24 h no DNA damage was present. SHSY-5Y cells,
however, did not repair their DNA, did not quench the oxidative stress, and showed characteristic signs that they were beginning
to undergo apoptosis. These results indicate that there is a differential response between this cancerous and normal cell
line in their ability to respond to low-dose IR, and these differences need to be exploited in order to treat cancer effectively.
Further study is needed in order to elucidate the mechanism by which SHSY-5Y cells undergo apoptosis following radiation and
why these normal cells are better equipped to deal with IR-induced double-strand breaks and oxidative stress. 相似文献
16.
Estimating attenuation of ultraviolet radiation in streams: field and laboratory methods 总被引:1,自引:0,他引:1
Belmont P Hargreaves BR Morris DP Williamson CE 《Photochemistry and photobiology》2007,83(6):1339-1347
We adapted and tested a laboratory quantitative filter pad method and field-based microcosm method for estimating diffuse attenuation coefficients (K(d)) of ultraviolet radiation (UVR) for a wide range of stream optical environments (K(d320) = 3-44 m(-1)). Logistical difficulties of direct measurements of UVR attenuation have inhibited widespread monitoring of this important parameter in streams. Suspended sediment concentrations were manipulated in a microcosm, which was used to obtain direct measurements of diffuse attenuation. Dissolved and particulate absorption measurements of samples from the microcosm experiments were used to calibrate the laboratory method. Conditions sampled cover a range of suspended sediment (0-50 mg L(-1)) and dissolved organic carbon concentrations (1-4 mg L(-1)). We evaluated four models for precision and reproducibility in calculating particulate absorption and the optimal model was used in an empirical approach to estimate diffuse attenuation coefficients from total absorption coefficients. We field-tested the laboratory method by comparing laboratory-estimated and field-measured diffuse attenuation coefficients for seven sites on the main stem and 10 tributaries of the Lehigh River, eastern Pennsylvania, USA. The laboratory-based method described here affords widespread application, which will further our understanding of how stream optical environments vary spatially and temporally and consequently influence ecological processes in streams. 相似文献
17.
The ICRP report recommends to include aircrew members among occupationally exposed persons. Many theoretical and experimental studies have been realized since 1991, and several international bodies have taken part in discussion of the topic. Most of these results are based on studies and analysis realized in the laboratory of the authors.Work partially supported by EC Project FI3P-CT92-0026. 相似文献
18.
The so-called concave or tailed survival curves are reported both for multi and single species bacterial populations. Taking as an example Bacillus pumilus, frequently encountered in decontamination studies, it is shown that the tailed survival curves are adequately described by stretched (0<<1) exponential SF=exp[−(D/D0)], where SF denotes the fraction of species surviving the irradiation dose D, D0 is the effective dose, and is the dispersion parameter interpreted phenomenologically in terms of radiation sensivity distribution for single species population under the given experimental conditions. 相似文献
19.
Yu L Cheng Q Cañas J Valentin-Blasini L Blount BC Anderson T 《Analytica chimica acta》2006,567(1):66-72
The ability to measure environmental contaminants in biological tissues and fluids is important in the characterization of exposure. However, the analysis of certain contaminants in these matrices presents significant challenges. Perchlorate (ClO4−) has emerged as a potential contaminant of concern primarily in drinking water and also in contaminated food. Significant advances have been made in the analysis of perchlorate in environmental matrices (water, soil) by ion chromatography (IC). In contrast, the analysis of perchlorate in extracts of biological tissues and fluids (vegetation, organs, milk, blood, urine, etc.) presents several challenges including small sample sizes, extracts with high matrix conductivity, and co-elution of other ions during IC analysis. To be able to detect low concentrations of perchlorate in biological samples, interferences must be removed or minimized, such as through the use of preparative chromatography cleanup techniques and/or alternative analytical methods less susceptible to common interferences (preconcentration or mass spectrometric detection). We present discussion and examples of the challenges encountered in the analysis of tissue extracts and fluids for perchlorate by IC and how some of those analytical challenges have been overcome. 相似文献
20.
Ryan LK Neldon DL Bishop LR Gilmour MI Daniels MJ Sailstad DM Selgrade MJ 《Photochemistry and photobiology》2000,72(4):497-507
Ultraviolet radiation (UVR) causes systemic immune suppression, decreasing the delayed type and contact hypersensitivity responses in animals and humans and enhancing certain mycobacterial, parasitic and viral infections in mice. This study tests the hypothesis that prior exposure to UVR enhances influenza infections in mice. BALB/c female mice were exposed to 0-8.2 kJ/m2 of UVR. Exposed and unexposed mice were infected intranasally three days later with 150-300 plaque-forming units/mouse (lethal dose (LD)20-LD40) of mouse-adapted Hong Kong Influenza A/68 (H3N2) virus or sham infected with 50 microL Hanks' balanced salt solution/mouse. Mortality from viral infection ranged from 25-50%. UVR exposure increased virus-associated mortality in a dose-dependent manner (up to a two-fold increase at 8.2 kJ/m2). The increased mortality was not associated with bacterial pneumonia. The highest dose of UVR also accelerated the body weight loss and increased the severity and incidence of thymic atrophy associated with influenza infection. However, UVR treatment had little effect on the increase in lung wet weight seen with viral infection, and, to our surprise, did not cause an increase in virus titers in the lung or dissemination of virus. The mice died 5-6 days after infection, too early for adaptive immune responses to have much impact. Also, UVR did not interfere with the development of protective immunity to influenza, as measured by reinfection with a lethal challenge of virus. Also, cells adoptively transferred from UVR or untreated mice were equally protective of recipient mice challenged with a lethal dose of virus. The mice resemble mice succumbing to endotoxin, and influenza infection increased the levels of tumor necrosis factor alpha (TNF-alpha) in bronchoalveolar lavage fluid and serum cortisol levels; however, UVR preexposure did not increase either of these responses to the virus. The results show that UVR increased the morbidity, mortality and pathogenesis of influenza virus in mice without affecting protective immunity to the virus, as measured by resistance to reinfection. The mechanism of enhanced mortality is uncertain, but the data raises concerns that UVR may exacerbate early responses that contribute to the pathogenesis of a primary viral infection. 相似文献