核桃楸皮挥发油化学成分分析

采用水蒸汽蒸馏法提取核桃楸皮中的挥发油,用气相色谱／质谱联用法,鉴定了39种化合物,其中分离出烃类(26种,71．80％)、酮类(3种,10．83％)、醇类(6种,7．96％)、呋喃类(1种,5．79％)、酚类(1种,1.99％)、肟类(1种,0.95％),酯类(1种,0．71％)7大类化合物,有3类(7种,33．93％)为已知药用成分,为进一步评价其质量和开发新药提供了基础数据。     

核桃楸叶挥发油化学成分分析

采用水蒸气蒸馏法提取核桃楸叶中的挥发油,从核桃楸叶挥发油中分离了57种化合物,用气相色谱/质谱联用法鉴定了51种化合物,占被分离化合物的89.46%,其中烃类(33种,48.03%)、酮类(1种,0.55%)、醇类(5种,25.94%)、酯类(6种,8.43%)、酸类(1种,0.62%)、含氧杂环类(4种,2.62%)及甲酰类(1种,0.65%)等7大类化合物,有3类(27种,51.19%)为已知药用成分,这些数据为新药开发提供了科学信息.     

高效液相色谱法测定柳树皮提取物中的水杨甙

 建立了一种测定柳树皮提取物中水杨甙的反相高效液相色谱法。该法采用KromasilC18(4 6mmi.d .× 2 5 0mm ,5 μm)色谱柱 ,以甲醇 0 0 1mol/LKH2 PO4 缓冲液 (pH 4 0 1) (体积比为 15∶85 )的混合溶液作流动相 ,流速为1 0mL/min ,紫外检测波长为 2 6 5nm ,灵敏度为 0 0 4AUFS。在 8 89mg/L～ 2 84 40mg/L的范围内 ,水杨甙的峰面积Y与其质量浓度X的线性关系良好 ,回归方程为Y =- 2 91 5 2 6 0 +5 0 0 0 0 34X(r =0 9998)。水杨甙的平均回收率为 96 1%～ 10 1 2 % (n =5 ) ,相对标准偏差 (RSD)为 1 43%。该法操作简便、快速、准确。     

刺梨提取物膜分离产品的成分分析及其在卷烟中的应用

为了提高刺梨提取物的质量,采用膜分离技术对其进行分离纯化,应用液质分析所得到的产品进行成分分析,并通过对这些产品的评吸,分析化学成分和烟用效果之间的关系.结果表明,膜分离技术在保留刺梨提取物原有特色香味的同时,可以有效去除负面影响烟气感官质量的大分子物质,达到提高刺梨提取物质量的目的.纯化后刺梨提取物的主要成分为有机酸类和多糖类化合物.添加在卷烟中,有着改善口感,柔和烟气的积极作用.     

唐古特大黄乙醇提取物的HPLC指纹图谱研究

用高效液相色谱法建立唐古特大黄乙醇提取物的指纹图谱分析方法。采用Phenomenex Kromasil C18色谱柱(4.6 mm×250 mm,5μm);甲醇-0.1%H3PO4为流动相,梯度洗脱,检测波长为270 nm,以大黄酸为参照峰。结果共有18个共有峰。此法为有效地控制唐古特大黄乙醇提取物的质量提供了依据。     

高效液相色谱法同时测定烟用甘草提取物中14种活性成分

0.100 0g的甘草提取物样品用10mL的80%(体积分数)甲醇溶液超声提取30min后静置30min,取上清液经0.45μm滤膜过滤。采用高效液相色谱法同时测定滤液中14种活性成分的含量,以Diamonsil Plus C18色谱柱为分离柱,用0.05%(体积分数)磷酸溶液和乙腈以不同比例混合的溶液为流动相进行梯度洗脱,用二极管阵列检测器测定。通过单因素试验和L9(33)正交试验对提取条件进行了优化。14种活性成分的质量浓度在一定范围内与其对应的峰面积呈线性关系,方法的检出限(3s)为0.02~0.12 mg·L^-1。在3个浓度水平进行加标回收试验,回收率为91.7%~143%,测定值的相对标准偏差(n=5)为1.2%~6.0%。     

反相高效液相色谱法用于葛根黄酮提取物的分离与主要活性成分的测定

采用反相高效液相色谱法，在C18柱上以甲酵—水为流动相进行梯度洗脱，在0-10min内将葛根黄酮提取物进行分离，并在250nm检测，采用标准加入法对实际样品中的葛根素含量进行了分析。结果表明：平均回收率为94.8％，RSD为2．4％。     

阔叶树皮木醋液与木焦油有机成分的气相色谱分析

运用气相色谱对阔叶树皮木醋液及木焦油水提取液的有机成分,如醇类、羧酸类、酮类、酚类等进行了定量分析.结果表明,木醋液随着蒸馏处理,甲醇、乙醇、丙酮等低沸点物质主要分布在前馏液中,2,6-二甲氧基苯酚、甲基环戊烯醇酮、2-甲基-3-羟基-4-吡喃酮等高沸点物质主要分布在残馏液中.通过阔叶树皮木醋液及木焦油水提取液的两种主馏分所含有机成分的定量分析比较,表明除甲醇、乙酸、丙酸、2-呋喃醛等成分以外,二者具有相似的化学成分组成.     

中药微量元素的主成分分析

基于主成分分析的方法,研究了中药的相似性。主成分综合得分越近,中药就越相似。     

Anthracene and Anthraquinone Derivatives from the Stem Bark of Juglans mandshurica Maxim.

One new anthracene derivative, juglanthracenoside A ( 1 ), two new anthraquinones, juglanthraquinone A ( 2 ) and juglanthraquinone B ( 3 ), along with a new naturally occurring anthraquinone, 9,10‐dihydro‐4,8‐dihydroxy‐9,10‐dioxoanthracene‐2‐carboxylic acid ( 4 ), have been isolated from the stem bark of Juglans mandshurica. Their structures were established by detailed spectroscopic analysis and comparison of the NMR data with those of related compounds. Compound 1 displayed noticeable antioxidant activity in both 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) free radical‐scavenging assays, while compound 4 showed strong cytotoxicity against HepG2, SGC7901, HCT‐8, and A549 cell lines in vitro.     

A new coumarin from Juglans mandshurica Maxim induce apoptosis in hepatocarcinoma cells

In this study, a new coumarin, juglansoside C (1) was isolated from the bark of Juglans mandshurica. Its chemical structure was identified by comprehensive spectroscopic analyses. The in vitro cytotoxicity assay showed that 1 exhibited moderate cytotoxicity against human hepatocellular carcinoma Hep3B cells with an IC₅₀ value of 70.9 μM. Furthermore, Annexin V-FITC/PI staining assay indicated that 1 markedly induced apoptosis in Hep3B cells.     

Two new tetralone glycosides from the green walnut husks of Juglans mandshurica Maxim

Two new tetralone glycosides, 4(S)-5-methoxy juglanoside A (1), 4(S)-5-methoxy juglanoside D (2), together with ten known compounds (3–12) have been isolated from the green walnut husks of Juglans mandshurica Maxim. Their structures were elucidated on the basis of their ESI-MS, 1?D and 2?D NMR spectroscopic data. In addition, all compounds were evaluated for their cytotoxic activities against the cancer BGC-823 (human gastric carcinoma), HCT-15 (human colorectal carcinoma) and K562 (human chronic myeloid leukemia) cell lines. The results showed aglycones of naphthoquinones had stronger cytotoxic activities than glycosides of tetralone.     

Chiral resolution and bioactivity of enantiomeric furofuran lignans from Juglans mandshurica Maxim

Abstract

Enantiomers have generally been reported mostly for racemic mixtures with a 1:1 ratio, as in that case there were weak Cotton effects in the ECD spectrum and negligible optical rotations. A furofuran lignan (sesamin), with a remarkable rotation and significant Cotton effects, was isolated from Juglans mandshurica Maxim. Subsequently, sesamin was resolved by chiral HPLC to afford a pair of enantiomers, (+)-sesamin (a) and (?)-sesamin (b), in a ratio of approximately 1:3. Their absolute configurations were determined by computational analysis of their electronic circular dichroism (ECD) spectrum. In addition, the pair of enantiomers were evaluated for the inhibition of self-induced Aβ aggregation. Interestingly, (+)-sesamin (a) (67.7%) and (?)-sesamin (b) (80.6%) exhibited different degrees of anti-Aβ aggregation activity. The different inhibition profiles were further explained by molecular dynamics and docking simulation study.     

A new naphthalenone isolated from the green walnut husks of Juglans mandshurica Maxim.

Phytochemical study of green walnut husks of Juglans mandshurica Maxim. led to the isolation of a new naphthalenone, (4R)-3,4-dihydro-4-butoxy-5-hydroxy-naphthalen-1(2H)-one (1), together with 16 known compounds. Compounds 4–6, 13, 14 and 17 were isolated from the genus Juglans for the first time, and their chemotaxonomic significance was also evaluated.     

α-Tetralone glycosides from the green walnut husks of Juglans mandshurica Maxim. and their cytotoxic activities

Abstract

Five new α-tetralone glycosides, juglanbiosides A-E (1–5), together with an α-tetralone derivative (15) and nine known 1,4-naphthoquinones (6–14) were isolated from the 95% EtOH extract of green walnut husks of Juglans mandshurica Maxim. Their structures were elucidated by comprehensive spectroscopic methods (¹H, ¹³C NMR, DEPT, HSQC, HMBC, CD, HR-ESI-MS). In vitro cytotoxicities of all the isolated compounds were evaluated against BGC-823, HCT-15 and K562 cancer cell lines.

     

A cytotoxic compound from the leaves of Juglans mandshurica

From Juglans mandshurica leaves, a new quinone compound was isolated through bioassay-guided fractionation. The structure elucidation of the compound was established based on spectroscopic studies, notably of the 2D NMR spectra. The compound exhibited moderate cytotoxic activities against Hela, MCF-7, BGC823 and 3T3-Llcell lines with IC50 ranges from 7.5 to 26.8 μmol/L.     

New alpha-tetralonyl glucosides from the fruit of Juglans mandshurica

Five new alpha-tetralonyl glucosides, juglanosides A-E (1-5) were isolated from the fresh rejuvenated fruit of Juglans mandshurica. Their structures were elucidated as (4S)-4-hydroxy-alpha-tetralone 4-O-beta-D-glucopyranoside (1), (4S)-4,5-dihydroxy-alpha-tetralone 4-O-beta-D-glucopyranoside (2), (4S)-4,6-dihydroxy-alpha-tetralone 4-O-beta-D-glucopyranoside (3), (4S)-4,5,8-trihydroxy-alpha-tetralone 4-O-beta-D-glucopyranoside (4), and (4S)-4,5,8-trihydroxy-alpha-tetralone 5-O-beta-D-glucopyranoside (5) on the basis of spectroscopic analysis and chemical evidence.     

Four new diarylheptanoids from the roots of Juglans mandshurica

Four new diarylheptanoids (1-4), along with two known tetralones (5, 6), were isolated from the roots of Juglans mandshurica and their structures were elucidated on the basis of spectroscopic studies.     

Flavonoids and Naphthoquinones from Juglans nigra Bark

Chemistry of Natural Compounds -