房水中烯醇水酶总活性的联酶荧光法测定

采用酶联反应荧光法测定样品烯醇化酶的总活性。方法最低检出限为０．２ＩＵ／Ｌ，在０．２０～３５ＩＵ／Ｌ范围标准曲线呈线性，γ〉０．９９７，回收率达９０％，相对标准偏差〈１０％，对临床确诊的视网膜母细胞瘤进行了初步探讨，检验结果垢特异性较高。     

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ＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳＴＮＳＴＲＵＣＴＩＯＮＳＴＯＯＮＴＲＩＢＵＴＯＲＳ￥／／ｔ．ａｃｉｓａｂａｔｈｌｙｉｏｕｓ＇ｎａｌ，ｗｔｌｉｓｈｅｄｂｙｔｈｅＣｈｉｎｅｓｅＣｈｏｎｉｃａｌＳｏｃｉｅｔｙ．ａｘｆｉ１１ｃａｌｉｐｈｐ...     

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ＴＮＳＴＲＵＣＴＩＯＮＳ　ＴＯ　ＣＯＮＴＲＩＢＵＴＯＲＳＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳ￥／／￣ｔｖｅｄ２６ｔｈｅｙ１９９５）ＴＮｅｓ￣ｏｒｘｃ＞ＷＨ￣ｃｏｗ￣Ｉ￣ｓｉｔ．ａｃｉｓａｏｏｎｔｈｌｙｊｏｉｎ＇ｎａｌ，ｍｕｌｉｓｈｅｄ...     

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ＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳ￥／／ｔ，ＣＣＬｉｓａｍｏｎｔｈｌｙｊｏｕｎａｌｐｕｂｌｉｓｈｅｄｏｙｔｈｅＣｈｉｎｅｓｅＣｈｅｍｉｃａｌＳｏｃｉｅｔｙ．ＣＣＬｗｉｌｌｐｕｂｌｉｓ...     

荧光法测定碱性磷酸酶及其标记物

本文研究了以４－甲基伞形酮磷酸酯（４－ＭＵＰ）为底物测定碱性磷酸酶（ＡＬＰ）活性的高灵敏荧光法及其应用于人血清ＩｇＧ的含量测定。通过纯化４－ＭＵＰ，优化分析条件，使方法的灵敏度有较大提高，实用性也得到改善，测定ＡＬＰ的线性范围为１．７×１０＾－５￣１．７×１０＾－８ＩＵ／ｍＬ，检出限为４．０×１０＾－９ＩＵ／ｍＬ，本法已成功地应用于血清样品ＡＬＰ的测定。作为一个例子，通过测定免疫标记物ＨｕＩｇＧ－     

INFLUENCE OF MOLYBDENUM ADDITIVE ONTO THE Co/CuLaZr CATALYSTS

ＩＮＦＬＵＥＮＣＥＯＦＭＯＬＹＢＤＥＮＵＭＡＤＤＩＴＩＶＥＯＮＴＯＴＨＥＣｏ／ＣｕＬａＺｒＣＡＴＡＬＹＳＴＳ￥ＷｅｉＣＨＵ；Ｒ．ＫＩＥＦＦＥＲ；Ａ．ＫＩＥＮＨＥＮＡＮＮ（ＬａｂｏｒａｔｏｉｒｅｄｅＣｈｉｍｉｅＯｒｇａｎｉｑｕｅＡｐｐｌｉｑｕｅｅ，ＵＡ...     

“消障灵”治疗早期老年性白内障疗效研究

对１０２例老年性白内障病人服药前后检测血中微量元素及红细胞酶活性．用高锌微量元素的中药内服，观察６个月～２年，结果视力增进７０．５％；血锌提高１．６μｇ／ｍＬ（Ｐ值＜０．００１），血红细胞过氧化酶提高４．８７ＩＵ／ｇＨｂ（Ｐ值＜０．００１），血红细胞谷胱甘肽还原酶提高４．３７ＩＵ／ｇＨｂ（Ｐ值＜０．００１）．说明锌对治疗早期老年性白内障有一定临床意义．     

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ＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳ￥／／ｔ．ａｘｉｓａ＂ａ）ｒｌｔｂｌｙｊｏｕｌ＇ｎａｌ，ｔｗｌｌｇｈｅｄ＇：：ｖ．ｔｈｅＣｈｉｎｅｄｓＣｆｌｏｎｉｃａｌＳｏｏｔｅｔｃ．ａｘａｉｌＩａｃａｌｉｓｈｐｅｅｌｉｍｉｎａｒｙａｃｃｃａｎｔ...     

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ＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳ￥／／ｔ．~ｉｓａｍｏｎｔｈｌｙＪｏｉｎ，ｎａｌ，ｃａｌｉｐｈｅｄｏｙｔｈｅＣｈｉｎｅｓｅＣｈｅｍｉｃａｌＳｏｏｔａｔｅ．ＣＣＬｙｉ１１ｃａｌｉｐｈｐｒｅｌｉｍｉｃａｒｙａｃｃｏｕｎｔｓｉｎｔｈｅａ?..     

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ＴＮＳＴＲＵＣＴＩＯＮＳＴＯＣＯＮＴＲＩＢＵＴＯＲＳ￥／／ｔ＇ａｔｉｓａｏｃｒｌｔｈｌｙＪｏｉｎ＊ｎａｌ，ｗｔｌｉｇｈｅｄｔｊｙｔｉｌｅＣｈｉｎｅｓｅＣｈｅｄｃａｌＳｏｏｔａｔｅ．ａｘａｉｌＩｐｏｂｌｉｓｈｐｅｅｌｉｍｉｎａｒｙａｅｅＬｉｍｔｓｉｎｔ...     

酸性接枝淀粉固定化糖化酶及稳定性研究

糖化酶(EC.3.2.1.3,GA)是工业上应用规模最大的3种酶制剂之一.目前,工业生产中大多采用游离GA,这给产物的分离和纯化带来诸多不便.因此,采用固定化糖化酶(IGA)是酶制剂工业发展的必然趋势.     

Novel Porous Polyethersulfone Beads as Matrix to Immobilize Comamonas testosteroni sp.bdq06 in Quinoline Biodegradation

Novel matrix beads for the immobilization of strain Comamonas testosteroni sp. bdq06 to degrade quinoline were fabricated from polyethersulfone(PES). The beads have an average size of 3 mm and a surface dense layer of 20 microns. To help adhesion and proliferation of bacterial cells, the surfaces of the PES beads were etched, and numerous holes about 1.5 micrometers in diameter were generated as tunnels for cell colonizing in the larger internal cavities of about 5 micrometers in diameter. The quinoline degradation was remarkably enhanced by the cells immobilized in PES beads compared with that by the free cells at pH 5.0 or 10.0 and a temperature of 40 ℃. The enhanced degradation of quinoline was contributed to the biofilm on the surface of PES beads, resulting in the significant reduction of retention time from 9 h to 2 h. Furthermore, the beads remain intact after the ultrasonic treatment of them for 30 min or recycling 50 times, indicating that they have excellent mechanical strength, flexibility and swelling capacity. Thus, PES beads have great potential to be matrix for the cell immobilization in bioaugmentation.     

The controlled release of a drug from biodegradable chitosan gel beads

Chitosan (CS) forms a gel in solutions with a pH above 12, and the gelation occurs at pH of about 9 in 10% amino acid solutions. In this paper, we investigated the enzymatic degradation and the drug release profile of this novel CS gel beads. The degradability of the CS gel beads was affected by the CS properties, e.g. the degree of deacetylation. The release of prednisolone (PS), as a model drug, from the CS gel beads was sustained significantly compared with the gel prepared with NaOH only. However, the release was not able to be sustained by the increment of NaOH concentration in the solution employed for the preparation of CS gel beads. We also investigated the control of drug release from CS gel beads by application of a complex formed between chondroitin sulfate (Cho) and CS. The release of PS from the CS gel beads treated with Cho was prolonged, and the release pattern was not affected by the treatment time. The time to 50% drug release was about 5 min with PS powder, about 200 min in CS gel beads with 10% glycine (Gly) (pH 9.0), and about 330 min in the CS gel beads with 10% Gly (pH 9.0) treated with Cho. Thus CS gel beads appear promising as a vehicle for sustained drug delivery, and the degradation of CS gel beads may be controlled by the degree of deacetylation of CS.     

Preparation and drug retention of biodegradable chitosan gel beads.

Chitosan (CS) gel beads containing drug could be prepared in amino acid solutions of pH about 9, despite the requirement for a pH above 12 for gelation in water. This phenomenon was observed not only in amino acid solutions but also in solutions of compounds having amino groups. A solute concentration of more than 10% was required for preparation of gel beads at pH 9. Gelation of the CS beads required about 25 to 40 min, depending on the species of amino acid. Lidocaine hydrochloride (LC) as a model drug was retained in the beads to about 20 to 35% of the theoretical total amount, despite being a water-soluble drug. The release of LC from the CS gel beads was prolonged. The release pattern was not affected by the species of amino acid or CS, or the preparation time.     

Release Property of Alginate Beads Coated with Poly(N-isopropylacrylamide-co-dimethylaminoethylmethacrylate)

Alginate beads were coated with copolymers of N-isopropylacrylamide and dimethylaminoethylmethacrylate (P(NIPAM-co-DMAEMA)s) by taking advantage of electrostatic interaction between alginate and the copolymer. The copolymers, of which DMAEMA contents in the feed for the copolymerization were 5.0% (PND 5) and 15.0% (PND 15), were employed for the coatings of alginate beads. The adsorption curve of PND 5 and PND 15 on the surface of alginate beads seemed to be Langmuir isotherm. The beads were coated in the PND solutions (2%, pH 5.0 or pH 7.0) and the amounts of adsorption (g PND/g alginate) were about 1.3 with PND 5 solution (pH 5.0), 1.2 with PND 15 solution (pH 5.0), and 0.75 with PND 15 (pH 7.0). The release from the beads coated with PNDs were observed at 30°C, 37°C, and 45°C using amaranth or FITC-dextran as a dye. The degrees of release were significantly suppressed due to the coating layers of PNDs. However, the coating layers could hardly act as a controller for the temperature-sensitive release.     

碳纳米管复合微球修饰L-色氨酸及其对LDL的吸附性能

本文以多孔碳纳米管/活性炭复合微球为载体, 以L-色氨酸为配基, 采用环氧氯丙烷偶联法, 制得修饰L-色氨酸的碳纳米管/活性炭复合微球(L-CNTs/AC)。采用扫描电镜、氮气吸附、傅立叶红外光谱、热分析、X射线光电子能谱等对复合微球进行表征;通过体外静态吸附法对其低密度脂蛋白(LDL)吸附能力进行初步研究。结果表明:环氧氯丙烷偶联法可接枝上L-色氨酸。复合微球中碳纳米管加入量越多, 对LDL的吸附能力越强;当碳纳米管加入量为45wt%时, 对LDL的吸附量达4.623 mg·g^-1, 是未添加碳纳米管的2.3倍多。这是因为碳纳米管不仅可促进复合微球中20~100 nm孔的形成, 而且还可促进复合微球配基修饰量的增多, 从而大大增强了复合微球对LDL的吸附能力。此复合微球可望开发成一种新型的血液灌流LDL吸附剂。     

Dual crosslinked pH‐ and temperature‐sensitive hydrogel beads for intestine‐targeted controlled release

Novel drug‐loaded hydrogel beads for intestine‐targeted controlled release were developed by using pH‐ and temperature‐sensitive carboxymethyl chitosan‐graft‐poly(N,N‐diethylacrylamide) (CMCTS‐g‐PDEA) hydrogel as carriers and vitamin B₂ (VB₂) as a model drug. The hydrogel beads were prepared based on Ca²⁺ ionic crosslinking in acidic solution and formed dual crosslinked network structure. The structure of hydrogel and morphology of drug‐loaded beads were characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). The study about swelling characteristics of hydrogel beads indicated that the beads had obvious pH‐ and temperature‐sensitivity. In vitro release studies of drug‐loaded beads were carried out in pH 1.2 HCl buffer solution and pH 7.4 phosphate buffer solution at 37°C, respectively. The results indicated that the dual crosslinked method could effectively control the drug release rate under gastrointestinal tract (GIT) conditions, which was superior to traditional single crosslinked beads. In addition, the effects of grafting percentage, pH value, and temperature on the release behavior of the VB₂ were investigated. The drug release mechanism of CMCTS‐g‐PDEA drug‐loaded beads was analyzed by Peppa's potential equation. According to this study, the dual crosslinked hydrogel beads based on CMCTS‐g‐PDEA could serve as suitable candidate for drug site‐specific carrier in intestine. Copyright © 2009 John Wiley & Sons, Ltd.     

Microparticle sampling by electrowetting-actuated droplet sweeping

This paper describes a new microparticle sampler where particles can be efficiently swept from a solid surface and sampled into a liquid medium using moving droplets actuated by the electrowetting principle. We successfully demonstrate that super hydrophilic (2 microm and 7.9 microm diameter glass beads of about 14 degrees contact angle), intermediate hydrophilic (7.5 microm diameter polystyrene beads of about 70 degrees contact angle), and super hydrophobic (7.9 microm diameter Teflon-coated glass beads and 3 microm size PTFE particles of over 110 degrees contact angles) particles on a solid surface are picked up by electrowetting-actuated moving droplets. For the glass beads as well as the polystyrene beads, the sampling efficiencies are over 93%, in particular over 98% for the 7.9 microm glass beads. For the PTFE particles, however, the sampling efficiency is measured at around 70%, relatively lower than that of the glass and polystyrene beads. This is due mainly to the non-uniformity in particle size and the particle hydrophobicity. In this case, the collected particles staying (adsorbing) on the air-to-water interface hinder the droplet from advancing. This particle sampler requires an extremely small amount of liquid volume (about 500 nanoliters) and will thus be highly compatible and easily integrated with lab-on-a-chip systems for follow-up biological/chemical analyses.     

Non-Destructive Analysis of European Cobalt Blue Glass Trade Beads

Chemical analyses were made of royal blue glass trade beads from two early 17th century, archaeological sites in southern Ontario, Canada and from a glass beadmaking house in Amsterdam, The Netherlands. The results confirm that these beads were all mixed alkali — lime — silica glasses, coloured with Co and with opaque varients opacified with Sn. The groupings by chemistry tend to segregate by bead shapes, so that oval beads group together and circular shaped beads group together. Although the 2 Canadian sites are about 190 km apart, they produced 2 different sets of oval beads of similar chemistry, possibly helping confirm the contemporaneity of the people at both sites. An As/Co atomic ratio of about two may fit with the possible source of Co as a cobalt-arsenide ore (of common name smaltite) from the Hartz Mountains of eastern Germany, a source not far from either Amsterdam or Venice, both well known glass beadmaking centres of the period.     

Dielectric properties of ion-exchange beads of sulfonic acid type dispersed in aqueous solutions — effect of fixed charge density

Ion-exchange beads with different densities of fixed charges were prepared by sulfoethylation of dextran gel beads. The relative permittivities and the electrical conductivities of the ion-exchange beads in a sodium form were evaluated by a dielectric technique consisting of the following two procedures proposed in a previous study (2): (a) Dielectric measurements for densely packed sediments of the ion-exchange beads; (b) Analysis of the observed dielectric relaxations by means of a theoretical equation of interfacial polarization for suspensions of spherical particles. The deduced permittivities of the ionexchange beads in equilibrium with water were about 60, which is lower than those of outer aqueous phases. The deduced conductirities were of the order of 1 to 10 mS cm^–1 and were increased reasonably with the fixed charge density. Closer consideration on these deduced values indicates that no specific interaction exists between the counter ions and the fixed charges irrespective of changes in fixed charge density and temperature.