共查询到19条相似文献,搜索用时 187 毫秒
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采用荧光光谱、紫外-可见光谱技术研究了芦丁与牛血清白蛋白(BSA)在金属离子Cu2+/CO2+共存时的相互作用.研究结果表明:有或无金属离子存在时,芦丁对BSA的荧光猝灭作用都是由于生成复合物而引起的静态猝灭;金属离子的存在使芦丁与BSA的表观结合常数KLB增大,且Co使KL)增大得更明显.由热力学参数确定芦丁和BSA... 相似文献
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Eu3+-芦丁配合物与DNA 相互作用的电化学和紫外可见吸收光谱研究 总被引:3,自引:0,他引:3
在0.1 mol·L-1乙酸缓冲溶液中(pH=5.69), 用电化学方法和吸收光谱法研究了Eu3+-芦丁配合物和小牛胸腺DNA的相互作用. 发现Eu3+-芦丁配合物与DNA通过静电吸引和沟槽键合模式键合. 在玻碳电极上通过循环伏安法和差示脉冲法得到了键合常数(K=1.42×104 mol·L-1)和键合位点数(n=2). 用紫外可见吸收光谱研究了Eu3+和芦丁的相互作用, 以及Eu3+-芦丁配合物与DNA 的相互作用. 相似文献
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运用二维相关红外光谱分析技术研究芦丁的热微扰过程 总被引:5,自引:0,他引:5
采用傅里叶变换红外光谱法(FTIR)和二维相关分析(2D correlation analysis)技术研究了固态芦丁20℃~160℃的升温过程。实验结果显示,芦丁红外光谱的特征峰在红外图上随着温度升高变化不明显,说明实验中芦丁分子没有发生氧化反应。借助于二维相关分析,提高了谱图的分辨率,将芦丁分子中不同苯环的骨架振动峰在二维同步图上显示并区分开来。同时还揭示了芦丁分子内各官能团之间的相互作用,反映了在热微扰过程中这些基因之间的协同关系和变化的先后顺序。分析结果表明;芦丁分子中两个苯环,杂环的C=O及=C-O-C基因发生了相互作用。其变化的顺序是和含氧杂环相连的苯环,C=O,=C-O-C和含氧杂环相并的苯环。此结论与芦丁的氧化反应机理相一致。总之,二维相关红外分析可以作为研究芦丁热微扰过程中结构动态变化的一种新方法,也为芦丁在氧化过程中的机理研究提供了一个重要的理论依据。 相似文献
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《化学研究与应用》2017,(6)
利用紫外-可见吸收光谱法研究了六、七、八元瓜环与芦丁的相互作用以及p H对其作用的影响,考察了包合物的体外缓释性能。结果表明,六、七元瓜环可以与芦丁形成1∶1的主客体包结物,八元瓜环与芦丁形成1∶2的包结物。包结平衡常数分别为3.6×10~5L·mol~(-1)、2.1×105L·mol~(-1)、1.9×10~(10)L~2·mol~(-2)。芦丁原药,Q[6]-芦丁、Q[7]-芦丁、Q[8]-芦丁包结物在人工胃液(p H=1.2)中60 min内的累计释放度分别为60.19%、46.71%、49.57、32.54%,在人工肠液(p H=6.8)中的累计释放度分别为73.76%、48.58%、51.32%、33.58%,且缓释曲线非常相似。实验结果表明,瓜环-芦丁主客体包结物的体外累积释放度明显低于芦丁原药,瓜环Q(n=6、7、8)对芦丁均有明显的体外缓释作用,且Q[8]对芦丁的缓释效果最为显著。 相似文献
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用支持向量回归方法(SVR)建立校正模型,并对维生素B1、B2和B6的模拟样品进行测定。结果表明,维生素B1、B2和B6预测结果的回收率在96%~104%之间,测定结果准确。 相似文献
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Electrochemical Studies of Pirarubicin and Its Interaction with DNA at a Co/GC Ion Implantation Modified Electrode 总被引:2,自引:0,他引:2
MA Xiao-mei HU Jing-bo ** LI Qi-long Department of Chemistry Beijing Normal University Beijing P. R. China 《高等学校化学研究》2004,20(6):751-756
IntroductionIon implantation is a new material surfacemodification technique.It has been also applied tostudying the electrochemical behaviors of organicdrugs and biological materials as well as their de-terminations. This method offers good stability,reproductivity and catalytic activity[1] .Pirarubicin( THP) is an active highly effective and new antitu-moral anthracycline antibiotic,which has gainedwidespread clinical use in the chemotherapeutictreatment of a variety of human cancers.The d… 相似文献
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镍离子注入修饰电极上米托蒽醌与DNA相互作用的电化学研究 总被引:6,自引:0,他引:6
研究了在镍离子注入修饰电极和0.05mol/LTris-0.5mol/LNaCl缓冲溶液(pH=7.1)中,米托蒽醌(MX)与DNA作用的电化学.在37℃恒温1.5h条件下,MX与DNA形成一种非电活性的结合物,使MX峰电流降低,其结合比n(MX):n(DNA)=2:1,结合常数为1.61×1012,电子转移系数为0.41,电极反应速率常数0.33s-1.加入DNA后,MX峰电流降低,据此,可以测定DNA. 相似文献
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In this paper, cyclic voltammetry, linear sweep voltammetry and chronocoulometry in connection with the hang mercury drop electrode were used to study NiTMpyP and its mixture with DNA. The reduction of NiTMpyP in our experimental conditions involves in 4e reduction of TMpyP. NiTMpyP interacting with DNA forms electrochemically non-active complex DNA-2NiTMpyP, which can not be reduced on the Hg electrode. The peak potential of NiTMpyP does not shift and its electrochemical kinetic parameters indicate no significant change in the presence of DNA. However, the reduction current of NiTMpyP decreases obviously due to the formation of DNA-2NiTMpyP, which implies its equilibrium concentration decreases when DNA was mixed. The decrease of peak current is proportional to DNA concentration, which can be applied to estimate DNA concentration. 相似文献
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Liansheng Duan Lijun Yang Huayu Xiong Xiuhua Zhang Shengfu Wang 《Mikrochimica acta》2013,180(5-6):355-361
We report on a glassy carbon electrode modified with carbon-coated nickel nanoparticles (C-Ni/GCE) that can be used to study the electrochemical properties of rutin and its interaction with bovine serum albumin (BSA) via cyclic voltammetry and differential pulse voltammetry. The effects of pH value, accumulation potential, accumulation time and reaction time were optimized. A pair of reversible peaks is found in the potential range of 0 to around 0.6 V at pH?5.0. Two linear response ranges (with different slopes) are found, one in the 2 to 210 nM concentration range, the other between 0.21 and 1.72 μM. The detection limit is as low as 0.6 nM. On addition of BSA to the rutin solution, a decrease of the current is observed that is proportional to the concentration of BSA. The binding constant and stoichiometric ratio were calculated. Figure
1. Preparation of carbon-coated nickel nanoparticles modified glassy carbon electrode (C-Ni/GCE). 2. C-Ni/GCE improves the electrochemical redox of rutin. 3. The prepared electrode determines rutin with high sensitivity and selectivity. 4. The developed method can determine rutin and its interaction with bovine serum albumin. 相似文献
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Electrochemical Detection of Rutin on Mg2Al‐Cl Layered Double Hydroxide Modified Carbon Ionic Liquid Electrode
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In this paper a Mg2Al‐Cl layered double hydroxide (Mg2Al‐LDH) modified carbon ionic liquid electrode (CILE) was prepared and further used for the electrochemical detection of rutin. Cyclic voltammograms of rutin on Mg2Al‐LDH/CILE were recorded with a pair of well‐defined redox peaks appeared in pH 2.5 phosphate buffer solution, which was ascribed to the electrochemical reaction of rutin. Due to the presence of Mg2Al‐LDH on the electrode surface, the redox peak currents increased greatly and the electrochemical parameters were calculated. Under the optimal conditions the oxidation peak current was proportional to rutin concentration in the range from 0.08 μmol L‐1 to 800.0 μmol L‐1 with the detection limit on 0.0255 μmol L‐1 (3σ). The fabricated electrode showed good reproducibility and stability, which was successfully applied to rutin tablet samples determination. 相似文献
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博莱霉素与DNA在镍离子注入修饰电极上的相互作用 及其应用 总被引:5,自引:0,他引:5
博莱霉素(BLM)在0.1mol/LHOAc-NaOAc缓冲溶液(pH4.62)中,在Ni/GCE离子注入修饰电极上有一灵敏的还原峰,峰电位为-1.16V(vs.SCE),峰电流与BLM浓度有关。用线性扫描和循环伏安法研究体系的行为表明,体系为具有加速作用的不可逆过程,是注入的Ni加速BLM的还原。引入DNA后,BLM的峰电位为-1.15V(vs.SCE),与未加入DNA前几乎完全一致;只使峰电流降低,形成一种非电活性的结合物,求得该结合物的结合比为BLM:DNA=3:1,结合常数为β=3.16×10^16,用线性扫描和循环伏安法,并辅以紫外可见光谱法等手段研究表明,电极过程仍为不可逆过程,与未加入DNA时一样。加入DNA后,BLM的峰电流降低,可用于DNA的测定,回收率在96.8~103.9%之间. 相似文献
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基于石墨烯分子印迹电化学传感器测定芦丁 总被引:2,自引:0,他引:2
将石墨烯(GR)滴涂至裸Au电极表面,并以邻氨基酚为功能单体,芦丁为模板分子,制备了芦丁分子印迹膜电化学传感器,利用循环伏安法(CV)和差分脉冲伏安法(DPV)对制得的传感器进行了电化学性能研究,并且对制备条件和测定条件进行了优化。结果表明,与裸Au电极相比,该GR修饰的Au电极在[Fe(CN)_6]~(3-/4-)溶液中峰电流明显增大,显著提高了芦丁分子印迹传感器的灵敏度。在最优实验条件下,基于GR分子印迹电化学传感器在4.40×10~(-6)~2.80×10~(-4) mol/L范围内呈良好的线性关系,检测限为1.46×10~(-6) mol/L。用该传感器测定了黑茶中芦丁的含量,获得较好结果。 相似文献
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《Analytical letters》2012,45(9):1843-1855
ABSTRACT The electrochemical behavior of the reduction of adriamycin at Ni/GC modified electrode has been studied by linear sweep and cyclic voltammetry. The reaction of DNA with ADM formed an electrochemically nonactive complex, which resulted in a decrease in the ADM equilibrium concentration and its reduction current. The decrease in peak current was proportional to DNA concentration and can be used to determination DNA concentration. The experiments of AES and XPS showed that Ni is surely implanted into the surface of GCE and the depth distribution of Ni was in good agreement with Gooses normal distribution; the implanted Ni at GCE improved the electrocatalytic activity. 相似文献