共查询到20条相似文献,搜索用时 185 毫秒
1.
分离蛋白质的高效离子交换色谱填料的研究(Ⅱ):新型弱阳… 总被引:3,自引:1,他引:3
将硅胶与环氧基有机硅烷反应的产物与聚乙烯亚胺交联,再与环状酸酐反应制得高效弱阳离子交换色谱填料WCX-5和WCX-6;交联产物与聚琥珀酰亚胺反应,然后碱性水解制得填料WCX-7,研究了它们的性能,WCX-5的分离性能优于WCX-5,WCX-7有一些不同于前两者的色谱特性,三者皆可用于蛋白质的分离,溶菌酶的活性回收率大于94%。 相似文献
2.
3.
4.
5.
6.
I_h对称性约化在SW-X_α方法中的实现与C_(60)的计算于微舟,张明瑜,李晓天(吉林大学理论化学研究所,理论化学计算国家重点实验室,长春,130023)关键词SW-X_α方法,对称性约化,电离能,UV光谱在合成出C(60)并发现它具有超导性质后,围... 相似文献
7.
在优良抗风湿药物萘普生的重要中间体6-甲氧基-2-乙酰素的合成中存在溶剂硝基苯的毒性及所带来的污染。而改用二氯乙烷作溶剂,只加少量硝基苯即可。为配合合成工艺的优化,用气相色谱(GC)、气相色谱-质谱(GC/MS)对反应产物、副产物及多个杂质进行了定性分析研究,并在5%OV-101ChromosorbWAWDMCS柱上完成了产物的内标定量分析。从而减少了副反应和高沸点杂质的生成,最终生产出了95%以上含量的6-甲氧基-2-乙酰萘产品,收率达到60%。 相似文献
8.
9.
用气相色谱、气相色谱-质谱对萘普生重要中间体6-甲氧基-2-乙酰萘合成反应的研究 总被引:3,自引:0,他引:3
在优良抗风湿药物萘普生的重要中间体6-甲氧基-2-乙酰素的合成中存在溶剂硝基苯的毒性及所带来的污染。而改用二氯乙烷作溶剂,只加少量硝基苯即可。为配合合成工艺的优化,用气相色谱(GC)、气相色谱-质谱(GC/MS)对反应产物、副产物及多个杂质进行了定性分析研究,并在5%OV-101ChromosorbWAWDMCS柱上完成了产物的内标定量分析。从而减少了副反应和高沸点杂质的生成,最终生产出了95%以上含量的6-甲氧基-2-乙酰萘产品,收率达到60%。 相似文献
10.
11.
12.
A weak cation-exchanger (XIDACE-WCX) has been synthesized by the indirect method. The chromatographic characteristics of the synthesized packing was studied in detail. The standard protein mixture and lysozyme from egg white were separated with the prepared chromatographic column. The chromatographic thermodynamics of proteins was studied in a wide temperature range. Thermodynamic parameters standard enthalpy change (deltaH0) and standard entropy change (deltaS0) and compensation temperature (beta) at protein denaturation were determined in the chromatographic system. By using obtained deltaS0, the conformational change of proteins was judged in the chromatographic process.The linear relationship between deltaH0 and deltaS0 can be used to identify the identity of the protein retention mechanism in the weak cation-exchange chromatography. The interaction between weak cation-exchanger and metal ions was investigated. Several metal chelate columns were prepared. The effects of introducing metal ion into the naked column on protein retention and the retention mechanism of proteins in the metal chalet affinity chromatography were discussed. 相似文献
13.
14.
钳蝎毒中抗癫痫肽、镇痛肽和抗肿瘤肽的快速同时分离和鉴定 总被引:7,自引:0,他引:7
采用Shim-Pack WCX-1型阳离子交换高压色谱柱对中国东亚钳蝎全蝎毒进行了分离,在鉴定了其中的抗癫痫肽、镇痛肽和抗肿瘤肽活性峰的基础上,应用Shim-Pack DIOL-300型凝胶排阻高压色谱柱对它们进行了进一步分离和鉴定,可以得到较纯的3种多肽。在高压色谱所提供的全蝎毒分离信息的基础上,应用与Shim-Pack WCX-1色谱柱具有相同交换基团的、具有较大吸附容量的CM Sepharose CL-6B软胶介质在低压色谱上对全蝎毒进行了分离,并分别对其中的抗癫痫肽、镇痛肽和抗肿瘤肽进行了鉴定。 相似文献
15.
利用国产大孔硅胶作基质合成了疏水填料。按照高效疏水作用色谱法,采用梯度洗脱方式分离了6种标准蛋白及唾液中α-淀粉酶和基因工程生产的γ-干扰素。柱子不可逆吸附小、被试验的α-淀粉酶和溶菌酶活性几乎定量被回收。应用合成的色谱填料研究了洗脱剂中盐浓度和温度对蛋白质保留行为的影响,论证了合成填料的色谱属性。 相似文献
16.
通过γ-巯丙基三甲氧基硅烷(KH-590)的作用, 将具有抗菌功能的中草药厚朴的主要药用成分厚朴酚键合在硅胶表面上, 制备了厚朴酚键合硅胶液相色谱固定相. 采用红外光谱、元素分析和热重分析对该固定相进行了表征. 以苯同系物、5种吡啶、6种苯胺和8种芳香羧酸类化合物为溶质探针, 初步考察了该新型固定相的基本色谱性能, 研究了其对这些化合物的保留机理. 结果表明, 该固定相的反相色谱性能类似于十八烷基键合硅胶固定相(ODS), 分离原理与疏水性作用有关; 另外, 该固定相包含有别于疏水性作用的氢键作用、π-π电荷转移作用和偶极-偶极等作用, 多种作用力使其在分离某些可电离的碱性和酸性化合物时表现出更好的选择性和分离效果. 厚朴酚配体的多种作用位点对快速分离极性芳香化合物有重要贡献. 相似文献
17.
《高分子科学杂志,A辑:纯化学与应用化学》2013,50(5):607-621
Uniform‐porous poly(dihydroxypropyl methacrylate‐co‐ethylene dimethacrylate), poly(DHPM‐co‐EDM) particles were synthesized as an alternative packing material for reversed phase chromatography. In the synthesis, poly(glycidyl methacrylate‐ethylene dimethacrylate), poly(GMA‐co‐EDM) particles were obtained by a multi‐stage swelling and polymerization protocol, the so called “modified seeded polymerization”. For this purpose, 2.4 µm polystyrene seed particles were first swollen by dibutyl phthalate (DBP) and then by a monomer mixture including glycidyl methacrylate and ethylene dimethacrylate. The repolymerization of monomer phase in the swollen seed particles provided porous uniform particles approximately 7 µm in size. Poly(DHPM‐co‐EDM) particles were obtained by the acid hydrolysis of the particles synthesized with different GMA feed concentrations. These particles were used as column‐packing material in the reversed phase separation of alkylbenzenes. The retention factor‐acetonitrile concentration diagrams clearly showed that the polarity of packing material could be controlled by changing the GMA feed concentration in the “modified seeded polymerization”. The packing materials with more hydrophobic character (i.e., poly(EDM) and poly(DHPM‐co‐EDM) particles produced with the GMA feed concentrations up to 20%) exhibited better chromatographic performance in the reversed phase mode. 相似文献
18.
A new hydrophilic strong anion-exchange (SAX) stationary phase for HPLC has been synthesized by chemical modification of macroporous 8.0-m monodisperse poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads (PGMA/EDMA). The stationary phase was evaluated in detail to determine its ion-exchange properties, separability, reproducibility, hydrophilicity, and the effect of column loading and pH on the separation and retention of proteins. It was found to have an ion-exchange chromatographic (IEC) retention mechanism. The highest dynamic protein loading capacity of the synthesized SAX packing for BSA was 22.6 mg g–1. Five proteins were separated within 6.0 min using the synthesized SAX resin. The SAX resin was also used for rapid separation and purification of recombinant human stem cell factor (rhSCF) from a crude extract solution in only one step. The purity of the purified of rhSCF was >92.4%. 相似文献
19.
Summary In an attempt to provide a more convenient approach towards better resolution of amino-acids (AAs) by RP-HPLC, a novel chromatographic
packing was prepared by dynamically coating 2-amino tetraphenyl porphyrin (atpp) onto a C18 reversed-phase packing. Its performance
was examined in order to arrive at the optimum operating conditions. Towards this end, the retention characteristics of 20
AAs that form the building blocks of proteins were investigated on both the C18 and the atpp coated C18 columns at different
pHs in presence and absence of Zn(II). The effects of eluent composition, pH and Zn(II) concentration were examined in each
case. The results obtained show that the atpp coated C18 column in the presence of Zn(II) ion shows retention times andk values that are effectively different from those of the C18 column and displays remarkable resolution for all of AAs, particularly
the polar and charged polar ones 12 out of 20 common AAs can be readily resolved under isocratic conditions. 相似文献